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Possible existence of tetrodotoxin-like toxins in cultured river puffer fish, Takifugu obscurus  

Kim, Do-Young (Bio-Evaluation Center, KIRBB)
Kim, Ju-Wan (Wando Maritime Office, Mokpo Regional Maritime Affairs and Port Office)
Park, Ki-Seok (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Kang, Hee-Woong (West Sea Fisheries Research and Development Institute, NFRDI)
Jeon, Joong-Kyun (Division of Marine Bioscience and Technology, College of Life Sciences, Kangnung National University)
Chung, Joon-Ki (Department of Aquatic Life Medicine, College of Fisheries Sciences, Pukyung National University)
Choi, Sang-Hoon (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Choi, Min-Soon (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Park, Kwan-Ha (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
Publication Information
Journal of fish pathology / v.22, no.1, 2009 , pp. 67-73 More about this Journal
Abstract
It was examined whether the common belief that "cultured puffer fishes do not contain tetrodotoxin (TTX)", the major lethal substance that accidently causes death in consumers of those fishes, is true in river puffer fish Takifugu obscurus. In mouse bioassay, lethal levels of toxins were detected in the ranks: gonad>liver>intestine>muscle>skin in wild puffer fish. In contrast, no mortality occurred in the mouse bioassay on cultured fish. However, there were sleepiness, sluggish behavior, and hind limb paralysis with the tissue extracts of cultured fish suggesting the presence of TTX or other similarly acting toxins. An attempt to confirm the presence of TTX in cultured fish with high performance liquid chromatography (HPLC) was not very successful. The results suggest possible existence of TTX toxins or similarly acting toxins.
Keywords
Tetrodotoxin (TTX); Bioassay; Wild puffer fish; Cultured puffer fish;
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