• The Korean Journal of Zoology
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• v.34 no.1
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• pp.31-43
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• 1991

In the present study, the localization of myosin and the pathway of myofibrilogenesis of skeletal muscle cells in culture were investigated by immunocytochemical methods including immunoelectron microscopy and hybridoma formation. There were manly free ribosomes in the cytosol of the myoblast. At 48 hr of the culture, the myofilaments started to form and at 72 hr began to assemble to form myoabrils. At this time the Z band appeared, but the sarcomeres did not link together. Aker 96 hr of culture, adjacent sarcomeres linked together and shotved the typical striation of the skeletal muscle fiber. Myosin was synthesized in free ribosomes at 24 hr of culture and localized at many myofilaments at 48 hr and assembled mvoabrils at 72 hr and at 120 hr of the culture, myosin was localized at thick filaments of the A band.

• Journal of the Korean Society for Precision Engineering
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• v.30 no.2
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• pp.236-240
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• 2013

In this presentation, we propose a fabrication method of PDMS stencil to apply into a localized culture of NIH/3T3 cells. PDMS stencil was fabricated by nitrogen gas blowing and soft lithography from preparing SU-8 master mold by photolithography. PDMS stencil pattern was production of the circle size 20 to $500{\mu}m$. In the culture test of PDMS stencil, a stencil was placed on a glass disk. The NIH/3T3 cells were successfully cultured into micropatterns by using the PDMS stencil. The results showed that cells could be cultured into micropatterns with precisely controlled manner at any shapes and specific size for bioscience study and bioengineering applications.

• Korean Institute of Interior Design Journal
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• v.21 no.5
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• pp.355-362
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• 2012

Water Culture Pavilion was constructed as a part of dam construction and Four-major rivers restoration projects, which have the purpose to prevent damage of natural disaster, localized heavy rain and drought, and has several functions; promotion, education and region culture community. Exhibition space in this culture pavilion should have the excellent connection of various media, contents, and exhibition space because of limited space. The purpose of this study is to analyze flows, continuation and connection of exhibit space with the perspectives of the attribute of water and to suggest various content things, technical, spatial types. This study targets Four-major rivers Water Culture Pavilion in Korea and suggests exhibition presentation methods as analyzing contents, media and constituent of exhibition space for each pavilion exhibition. The result of this study is as follows : First, the circulation is common expressed attribute of water in these four water culture pavilion. The reason is that there is a connection between Four-major rivers restoration projects and the physical attribute of water circulating the steps of evaporation, condensation and precipitation. Second, each pavilion presents circulative solid exhibit, circulative background exhibit, circulative reflective exhibit based on circulation. These three types of exhibition is related the floor separation. Third, each pavilion exhibit zone shows the most circulation, solid, background, reflexibility through educational contents and promoting contents by using graphic, video, sound media.

• Knowledge Management Research
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• v.13 no.2
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• pp.19-35
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• 2012

This study empirically investigates the differences of dominant management problems (hereafter, DMPs) among transplants in different degree of localization, based on the exploratory case study on the nine transplants of Hyundai/Kia suppliers in US using AHP(Analytic Hierarchy Process) technique. On the results of the literature review, this study divides the DMPs of transplants into three main categories such as building human infrastructure, stabilizing manufacturing processes, and building learning network. Each categories is also divided into four subcategories. The degree of localization includes two variables such as the localization stage and the employee size of transplant. Main findings are as follows. First, 'Communication among Korean and local employees' is important DMP in all transplants examined. Second, 'Local adaptation of Korean manufacturing technology' and 'Education on the Korean culture and management practices' are more important DMPs for less-localized transplants than more-localized ones. On the contrary, 'Motivating local employees' is more important DMP for more-localized transplants than less-localized ones. Third, 'Education on the technology and quality' is more important DMP for transplants in post-production stage than in stabilization stage. On the contrary, 'Staffing local employees' is more important DMP for transplants in stabilization stage than in post-production stage. Fourth, 'Acquiring test and measurement equipments' is more important DMP for small-sized transplants than large-sized ones. On the contrary, 'adopting new technology' and 'Building local suppliers network' are more important DMPs for large-sized transplants than small-sized ones.

• Development and Reproduction
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• v.18 no.3
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• pp.153-160
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• 2014

Adaptive development of early stage embryo is well established and recently it is explored that the mammalian embryos also have adaptive ability to the stressful environment. However, the mechanisms are largely unknown. In this study, to evaluate the possible role of aquaporin in early embryo developmental adaptation, the expression of aquaporin (AQP) 5 gene which is detected during early development were examined by the environmental condition. To compare expression patterns between in vivo and in vitro, we conducted quantitative RT-PCR and analyzed localization of the AQP5 by whole mount immunofluorescence. At in vivo condition, Aqp5 expressed in oocyte and in all the stages of preimplantation embryo. It showed peak at 2-cell stage and decreased continuously until morula stage. At in vitro condition, Aqp5 expression pattern was similar with in vivo embryos. It expressed both at embryonic genome activation phase and second mid-preimplantation gene activation phase, but the fold changes were modified between in vivo embryos and in vitro embryos. During in vivo development, AQP5 was mainly localized in apical membrane of blastomeres of 4-cell and 8-cell stage embryos, and then it was localized in cytoplasm. However, the main localization area of AQP5 was dramatically shifted after 8-cell stage from cytoplasm to nucleus by in vitro development. Those results explore the modification of Aqp5 expression levels and location of its final products by in vitro culture. It suggests that expression of Aqp5 and the roles of AQP5 in homeostasis can be modulated by in vitro culture, and that early stage embryos can develop successfully by themselves adapting to their condition through modulation of the specific gene expression and localization.

• Tuberculosis and Respiratory Diseases
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• v.67 no.5
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• pp.395-401
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• 2009

As the prevalence of tuberculosis declines, the proportion of nontuberculous mycobacterial (NTM) lung disease is increasing in Korea. The combined use of liquid and solid media increases the sensitivity of mycobacterial culture and shortens culture time. Because NTMs are ubiquitous in the environment, NTM lung disease requires strict diagnostic criteria to prevent over-diagnosis of NTM lung disease. Mycobacterium avium complex is the most common pathogen of NTM lung disease in Korea and present in two forms: upper lobe cavitary and nodular bronchiectatic form. Decision of treatment of NTM lung disease depends on the infecting species and overall condition of the patient. Because medical therapy requires the use of multiple drugs over 18 to 24 months, surgery for localized disease may be useful for those species refractory to medical therapy.

• Journal of the Korean Society of Food Culture
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• v.34 no.2
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• pp.184-200
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• 2019

This study aims to establish a strategy for selling kimchi to satisfy the needs and wants of European consumers through survey, FGI, and IDI. All survey participants were Europeans and were selected on the basis of their kimchi awareness. For the quantitative survey, 246 participants 15 years or older were selected; for FGI, 33 participants aged between 20 and 49 were selected; and for IDI, eight chefs were selected. The survey included four steps of 'Awareness-First try-Needs-Future consumption'. The participants got to know kimchi through the Internet; for example, by visiting Korea and Hallyu, and suggested positive comments, including 'fancy' and 'exotic.' While satisfaction with kimchi sharply decreased due to taste and appearance in the first try of kimchi, it is important to provide the best first-try experience. It also showed that important needs for kimchi are 'hot sensation', 'crunchiness' and 'presentation'. They gave a negative answer to combination with traditional cuisines of their own country, but provided positive comments for benchmarking the use of traditional pickled foods. This study suggests that it may be necessary to implement a two-track strategy by the traditional kimchi as a 'strategic product' and localized kimchi as a 'key product'.

• Journal of the Korean Society of Costume
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• v.58 no.4
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• pp.155-170
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• 2008

Club culture is the global trend for youth in 21st century. Club is the space that is created with close relationship among music, dance and fashion. It is also experimental cultural art space with endlessly transforming style. Furthermore it is a space for independent minor culture which represents speciality than generality of cultural appetite and style of club. Cultural communities formed around club and their parties have placed as a strong subculture trend based on youth age group. What they are creating as a subculture could be our tomorrow's main trend and clubbers also could be our major power sources for future. Therefore it is necessary to pay attention to club culture. The purposes of this research are to identify the concept of clubber, to analyzes their basic club culture characteristics and elements, and to find out unique fashion styles of Korean clubber in comparison with the origin. To study club fashion style's origin and background, this study searched a theoretical flow from 1930's to 1990's. Then, Korean clubber's style is derived by comparing background and origin of Korean club culture with those of abroad. To analyze in various point of view, theoretical backgrounds about social, cultural, dresses, and design were considered. Since research target is a visual image, street fashion is analyzed on through, music channels and magazines from 1930's to present as well as designer's art photographies. Internet sites', cub culture association's and sound association's photos were also extracted. as a visual evidences to offer actual evidences. Geological targets are selected among Korean club culture's origin such as Hong-Ik University area, Shin-chon, Chungdam-dong and Apgujung-dong areas. The results of this study are as follows. Firstly, clubber's fashion style influenced magnificently on major fashion design instead of being just youngster's resistance toward control group and it is contributing to our fashion culture to enrich it. Secondly, fashion styles of korean clubbers are based on those of western sub-culture, but with a unique localized history.

• Journal of the Korean Society of Food Culture
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• v.26 no.4
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• pp.383-393
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• 2011

This study aimed to identify efficient methods for the globalization of Korean food in Vietnam. For this, we interviewed owners of 11 Korean restaurants in Hanoi and 9 Korean restaurants in Ho Chi Minh. Due to the rapid economic development of Vietnam, the restaurant industry for the middle class has drastically increased. For the globalization of Korean food, new positioning of Korean restaurants is needed. Research has shown that Korean restaurants need to change their style of management. Although the first generation of Korean restaurant owners managed restaurants as a family business, a new professional management system is now required. Above all, it is necessary to develop and support a comprehensive food culture system in which the menu is suited to the taste of the natives and its recipe is distributed with food material quality control, hygienic control, and operation of a localized management system.

• Fisheries and Aquatic Sciences
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• v.8 no.3
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• pp.118-121
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• 2005

Arylsulfatase cloned from a marine bacterium, Pseudoalteromonas carrageenovora, was over-expressed in Escherichia coli. Most of the recombinant arylsulfatase was found in the cell lysate with induction up to $10{\mu}M$ IPTG. However, enzyme activity was observed both in the culture supernatant and cell lysate by induction with IPTG concentration of $50-5,000{\mu}M$. Most of the recombinant enzyme was localized in the periplasmic space with $10{\mu}M$ IPTG induction, while half of the enzyme was distributed in the periplasmic space with $50{\mu}M$ IPTG induction. Cell growth and arylsulfatase activity did not change with the induction time, and the level of recombinant arylsulfatase expression was maintained at 4-5 U/mL after 6 to 14 hr of culture.