Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Overexpression of Recombinant Arylsulfatase Cloned from Pseudoalteromonas carrageenovora

  • Kim Jong-Oh (Division of Food Science and Biotechnology, Pukyong National University) ;
  • Kim Seok-Ryel (Division of Food Science and Biotechnology, Pukyong National University) ;
  • Lim Jae-Myung (Department of Biotechnology and Bioengineering, Dong-Eui University) ;
  • Nam Soo-Wan (Department of Biotechnology and Bioengineering, Dong-Eui University) ;
  • Kim Hyeung-Rak (Division of Food Science and Biotechnology, Pukyong National University)
  • Published : 2005.09.01
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Abstract

Arylsulfatase cloned from a marine bacterium, Pseudoalteromonas carrageenovora, was over-expressed in Escherichia coli. Most of the recombinant arylsulfatase was found in the cell lysate with induction up to $10{\mu}M$ IPTG. However, enzyme activity was observed both in the culture supernatant and cell lysate by induction with IPTG concentration of $50-5,000{\mu}M$. Most of the recombinant enzyme was localized in the periplasmic space with $10{\mu}M$ IPTG induction, while half of the enzyme was distributed in the periplasmic space with $50{\mu}M$ IPTG induction. Cell growth and arylsulfatase activity did not change with the induction time, and the level of recombinant arylsulfatase expression was maintained at 4-5 U/mL after 6 to 14 hr of culture.

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References

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