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http://dx.doi.org/10.7736/KSPE.2013.30.2.236

Fabrication of PDMS Stencil using Gas Blowing for Micropatterned 3T3 Cell Culture  

Choi, Jin Ho (School of Mechanical Engineering, Kyungpook National University)
Kim, Gyu Man (School of Mechanical Engineering, Kyungpook National University)
Publication Information
Journal of the Korean Society for Precision Engineering / v.30, no.2, 2013 , pp. 236-240 More about this Journal
Abstract
In this presentation, we propose a fabrication method of PDMS stencil to apply into a localized culture of NIH/3T3 cells. PDMS stencil was fabricated by nitrogen gas blowing and soft lithography from preparing SU-8 master mold by photolithography. PDMS stencil pattern was production of the circle size 20 to $500{\mu}m$. In the culture test of PDMS stencil, a stencil was placed on a glass disk. The NIH/3T3 cells were successfully cultured into micropatterns by using the PDMS stencil. The results showed that cells could be cultured into micropatterns with precisely controlled manner at any shapes and specific size for bioscience study and bioengineering applications.
Keywords
Gas Blowing; PDMS Microstencil; Cell Culture; Photolithography; Micro Patterning;
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Times Cited By KSCI : 3  (Citation Analysis)
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