DOI QR코드

DOI QR Code

Fabrication of PDMS Stencil using Gas Blowing for Micropatterned 3T3 Cell Culture

가스 블로잉을 이용한 PDMS 스텐실 제작 및 3T3 세포의 마이크로 패터닝

  • Choi, Jin Ho (School of Mechanical Engineering, Kyungpook National University) ;
  • Kim, Gyu Man (School of Mechanical Engineering, Kyungpook National University)
  • Received : 2012.03.16
  • Accepted : 2012.07.19
  • Published : 2013.02.01

Abstract

In this presentation, we propose a fabrication method of PDMS stencil to apply into a localized culture of NIH/3T3 cells. PDMS stencil was fabricated by nitrogen gas blowing and soft lithography from preparing SU-8 master mold by photolithography. PDMS stencil pattern was production of the circle size 20 to $500{\mu}m$. In the culture test of PDMS stencil, a stencil was placed on a glass disk. The NIH/3T3 cells were successfully cultured into micropatterns by using the PDMS stencil. The results showed that cells could be cultured into micropatterns with precisely controlled manner at any shapes and specific size for bioscience study and bioengineering applications.

Keywords

References

  1. Tamura, T., Sakai, Y., and Nakazawa, K., "Twodimensional microarray of HepG2 spheroids using collagen/polyethylene glycol micropatterned chip," J. Mater. Sci.: Mater. Med., Vol. 19, pp. 2071-2077, 2008. https://doi.org/10.1007/s10856-007-3305-1
  2. Ilkhanizadeh, S., Teixeira, A. I., and Hermanson, O., "Inkjet printing of macromolecules on hydrogels to steer neural stem cell differentiation," Biomaterials, Vol. 28, pp. 3936-3943, 2007. https://doi.org/10.1016/j.biomaterials.2007.05.018
  3. Kim, G. M., van den Boogaart, M. A. F., and Brugger, J., "Fabrication and application of a full wafer size micro/nanostencil for multiple length-scale surface patterning," Microelectronic Engineering, Vol. 67-68, pp. 609-614, 2003. https://doi.org/10.1016/S0167-9317(03)00121-7
  4. Kim, G. M. and Brugger, J., "Fabrication of Miniaturized Shadow-mask for Local Deposition," J. Korean Soc. Precis. Eng., Vol. 21, No. 8, pp. 152-156, 2004.
  5. Kim, G. M., Chung, S. I., and Oh, H.-S., "Fabrication of nanostencil using FIB milling for nanopatterning," J. Korean Soc. Precis. Eng., Vol. 23, No. 3, pp. 56-60, 2006.
  6. Khetani, S. R. and Bhatia, S. N., "Microscale culture of human liver cells for drug development," Nat. Biotechnol., Vol. 26, No. 1, pp. 120-126, 2008. https://doi.org/10.1038/nbt1361
  7. Cho, C. H., Park, J., Tilles, A. W., Berthiaume, F., Toner, M., and Yarmush, M. L., "Layered patterning of hepatocytes in co-culture systems using microfabricated stencils," BioTechniques, Vol. 48, No. 1, pp. 47-52, 2010. https://doi.org/10.2144/000113317
  8. Fukuda, J., Khademhosseini, A., Yeo, Y., Yang, X., Yeh, J., Eng, G., Blumling, J., Wang, C.-F., Kohane, D. S., and Langer, R., "Micromolding of photocrosslinkable chitosan hydrogel for spheroid microarray and cocultures," Biomaterials, Vol. 27, pp. 5259-5267, 2006. https://doi.org/10.1016/j.biomaterials.2006.05.044
  9. Choi, J. H. and Kim, G. M., "Micro-Patterning on Non-Planar Surface using Flexible Microstencil," Int. J. Precis. Eng. Manuf., Vol. 12, No. 1, pp. 165-168, 2011. https://doi.org/10.1007/s12541-011-0023-x
  10. Folch, A., Jo, B.-H., Hurtado, O., Beebe, D. J., and Toner, M., "Microfabricated elastomeric stencils for micropatterning cell cultures," Journal of Biomedical Materials Research, Vol. 52, pp. 346-353, 2000. https://doi.org/10.1002/1097-4636(200011)52:2<346::AID-JBM14>3.0.CO;2-H
  11. Kang, J. H., Um, E., and Park, J.-K., "Fabrlcation of a poly(dimethylsiloxane) membrane with well-defined through-holes for three-dimensional microfluidic networks," J. Micromech. Microeng., Vol. 19, No. 4, Paper No. 045027, 2009.
  12. Choi, J. H., Lee, H., Jin, H. K., Bae, J. S., and Kim, G. M., "Micro patterning of MEF cell with microstencil," Proc. of KSPE Spring Conference, pp. 149-150, 2010.

Cited by

  1. Efficient and reliable screening of anti-obesity agents on a micro-cell pattern chip vol.91, pp.10, 2016, https://doi.org/10.1002/jctb.4876