• Title/Summary/Keyword: leaves extracts

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Antioxidant Activity of Extracts from Akebia quinata Decne

  • Rim, A-Ram;Kim, Sun-Jung;Jeon, Kyung-Im;Park, Eun-Ju;Park, Hae-Ryong;Lee, Seung-Cheol
    • Preventive Nutrition and Food Science
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    • v.11 no.1
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    • pp.84-87
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    • 2006
  • Antioxidant activity of Akebia quinata Decne was evaluated. Water extract (0.5 g/50 mL) of flowers and leaves of A. quinata were prepared and total phenol contents and radical scavenging activity of the extracts was determined for antioxidant activity. The total phenol contents of extracts from A. quinata flowers (FAQ) and leaves (LAQ) were $30.05{\mu}M\;and\;20.23{\mu}M$, while the radical scavenging activity of FAQ and LAQ were 60.51 % and 52.97%, respectively. In addition, the effect of FAQ and LAQ extract on DNA damage induced by $H_2O_2$ in human lymphocytes was evaluated by comet assay. The FAQ and LAQ showed strong inhibitory effect against DNA damage induced by $200{\mu}M$ of $H_2O_2$. These results suggest that water extracts of A. quinata Decne flowers and leaves showed significant (p<0.05) antioxidant activity and protective effect against oxidative DNA damage.

Free radical scavenging activity of some Mangroves available in Bangladesh

  • Ahmed, Firoj;Shahid, IZ;Razzak, MA;Rahman, M Mostafizur;Hoque, Tahmina;Rahman, MT;Sadhu, SK
    • Advances in Traditional Medicine
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    • v.6 no.1
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    • pp.58-64
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    • 2006
  • The crude alcoholic extracts of various parts of five different Bangladeshi mangrove plants (Amoora cucullata, Caesalpinia bonducella, Cerbera odollam, Derris uliginosa and Sonneratia caseolaris) were screened in-vitro for anti-oxidant activity using the 1,1-diphenyl-2-picryldydrazyl-hydrate (DPPH) free radical scavenging assay. Of all of them, the ethanolic extracts of the leaves of Derris uliginosa and Sonneratia caseolaris showed potential antioxidant activity ($IC_{50}$: 94.406 and 26.30 mg/ml respectively) whereas the ethanolic extracts of aerial parts of Derris uliginosa, barks of Cerbera odollam and leaves and stems of Caesalpinia bonducella showed moderate activity ($IC_{50}$: 125.89, 211.35 and 301.99 mg/ml respectively). Mild anti-oxidant activity was observed with the methanolic extract of leaves of Amoora cucullata and ethanolic extracts of fruits of Cerbera odollam.

Insecticidal activity of native plant extracts against Culix pipiens pallens and Musca domestica (자생식물 추출물의 모기 및 집파리에 대한 살충활성)

  • Kyung, Suk-Hun;Yoon, Young-Hee
    • The Korean Journal of Pesticide Science
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    • v.3 no.1
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    • pp.46-50
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    • 1999
  • Methanol extracts of 9 kinds of native medicinal plants(Taraxacum platycarpum, leaf; Pinus densiflora, leaf; Artemisia prinseps, leaf; Allium tuberosum, leaf; Cassia obtussifolia, whole; Sophora angestifolia, root; Stemonae sessilifolia, root; Lonicera japonica stem, leaf, flower; and Clivia miniata) were investigated for insecticidal effect. Methanol extracts of Pinus densiflora leaves and Sophora angestifolia radix showed relatively good insecticidal activity against Culex pipiens pallens larvae. Strong larvicidal activity against the Musca domestica larvae was produced from methanol extracts of Taraxacum platycarpum leaves and Allium tuberosom leaves. while Stemonae radix showed moderate activity. All materials tested revealed little or weak insecticidal activity against M. domestica adults.

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Phenolic contents and antioxidant activities of extracts from young leaves of Camellia japonica L. in Jeju (제주 동백나무 어린 잎의 페놀류 함량과 항산화 효능)

  • Jeong, Dong Won;Kim, Min Young
    • Journal of Applied Biological Chemistry
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    • v.60 no.4
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    • pp.307-311
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    • 2017
  • This study has been performed to identify the phenolic contents and antioxidant properties of methanol and ethanol extracts of young Camellia leaves in Jeju. Total polyphenol and flavonoid contents were 74.9 and 46.1 mg gallic acid equivalent/100 g, and 30.6 and 21.4 mg RE/100 g in the methanol and ethanol extracts, respectively. Based on $IC_{50}$ values, both extracts were also found to possess potent scavenging capacities of 1,1-diphenyl-2-picrylhydrazyl, nitric oxide and superoxide anion radicals, and ferrous ion chelating and reducing power activities. These results showed that young Camellia leaves is a good antioxidant source and holds promise as a natural ingredient in functional food and pharmaceutical supplement.

Antioxidant and Anticancer Effects of Agrimony (Agrimonia pilosa L.) and Chinese Lizardtail (Saururus chinensis Baill) (짚신나물, 삼백초의 항산화와 항암활성 효과)

  • Seo, Hun-Seok;Chung, Bong-Hwan;Cho, Yong-Gu
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.3
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    • pp.139-143
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    • 2008
  • The antioxidant activities of Agrimony (Agrimonia pilosa L.) and Chinese lizardtail (Saururus chinensis Baill) according to extraction methods were measured. SOD-like activity showed greater antioxidant effects with ethanol solvent than those with water. Ethanol extracts of Agrimony leaves showed the highest SOD-like activity of 94.4%. SOD-like activity differed according to the extraction solvents. The contents of polyphenolic compounds were higher in water extracts than those in ethanol extracts. The contents were 161.4 mg for Agrimony roots, 100.2 mg for Agrimony leaves, and 79.1 mg for Agrimony stalks in order. EDA in Agrimony leaves that were highest among medicinal plants were 83.4% in the water extract and 81.7% in the ethanol extract. The anticancer effects of the extracts by water and ethanol from Agrimony and Chinese lizardtail were experimented. The growth of stomach cancer cells, SNU-719 was inhibited 94.5% by the hexane fractions of Agrimony and also the growth of liver cancer cells, Hep3B was inhibited 83.2% by the hexane fractions of Agrimony, while the growth of normal cell, DC2.4 was not affected.

Nutritional Properties and Biological Activities of Artemisia annua L. (개똥쑥의 영양적 특성 및 생리활성)

  • Ryu, Ji-Hyun;Kim, Ra-Jeong;Lee, Soo-Jung;Kim, In-Soo;Lee, Hyun-Ju;Sung, Nak-Ju
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.2
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    • pp.163-170
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    • 2011
  • The nutritional properties and biological activities of leaves and stems of Gaeddongssuk (Artemisia annua L.) were investigated. Contents of moisture, crude lipid and crude protein were significantly higher in the leaves, and then ash, crude fiber and mineral were significantly higher in the stems. Contents of total phenols and flavonoids of leaves were about 2 fold higher than those of stems. Antioxidant activity was significantly increased in a dose-dependent manner; also, water and ethanol extracts of leaves were stronger than those of stems. Especially, DPPH radical scavenging activity, reducing power and tyrosinase inhibition activity were significantly higher in leaves extracts than stems extracts of Gaeddongssuk. But, ${\alpha}$-glucosidase inhibition activity was higher in stem than its leaves extract. In MTT assay by human breast adenocarcinoma cell line MCF-7 and MDA-MB-231, ethanol extracts of leaves showed the highest anticancer activity; the rates of growth inhibition were 76.26% and 52.59% on MCF-7 and MDA-MB-231 cells, at the concentration of $250\;{\mu}g$/mL, respectively. In conclusion, biological activities of extracts from Gaeddongssuk were dependent on the fiber, phenolic and flavonoid content.

Effect of Allium victorialis var.platyphyllum Leaves on Triton WR-1339-induced and Poloxamer-407-induced hyperlipidemic Rats and on Diet-Induced Obesity Rats (산마늘 기능성 활성물질 탐색 및 자원화)

  • Park, Hui-Jun;Choi, Jong-Won;Lee, Kyung-Tae;Kim, Won-Bae;Park, Kwang-Kyun;Lee, Jin-Ha;Lim, Sang-Cheol;Jung, Hyun-Ju;Park, Hee-Juhn
    • Proceedings of the Plant Resources Society of Korea Conference
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    • v.18 no.1
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    • pp.5-16
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    • 2005
  • The herb of Allium victorialis var. platyphyllum (Liliaceae) has been used as an edible wild herb and to treat heart failure and gastritis. We have already reported antihyperlipidemic anti-tumor effects of this plant. To enlarge the commercial availability of this food, it was investigated whether the extracts of A. victorialis var.platyphyllum reduce hyperlipidemia and obesity or not. The plants tested in this experiment were collected from two eco-types of IS. Ullung and Mt. Odae cultivated at Pyongchang. Extracts were prepared by extracting the fresh leaves and those dried at $36^{\circ}C$ and $90^{\circ}C$, respectively. Pretreatment with the ethanolic extracts for two weeks (p.o.) reduced serum triglyceride-, total cholesterol- and LDL-cholesterol contents in rats induced by Triton WR-1339, respectively. Furthermore, oral administration of the extracts also inhibited the hyperlipidemia induced with oral diet of 30% corn oil. In the other attempt to find to alleviate the obesity, the model rats with obesity were induced by the high fat-diet for six weeks. Post-treatment with the extracts for two weeks significantly reduced the hyperlipidemia. Retroperitoneal-, epidymal- and total abdominal fat pad weights were considerably reduced at 100 mg/kg oral administration of the extracts. Increased feces lipid contents were also found in the rat treated with the extracts. The extract of Mt. Odae eco-type showed more potent activity than that of Is. Ullung one. These results suggest that use of the fresh leaves may lead to the higher activity in treatment of hyperlipidemia and obesity than of the dried one.

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Antibacterial Activities of Ginkgo Biloba Leaves Extracts Against Isolated Bacteria from Museums (박물관에서 분리된 세균에 대한 은행잎 추출물의 항균활성)

  • Kwon, Young-Suk;Cho, Hyun-Hok;Jeong, Seong-Yun;Lee, Sang-Youb;Kim, Min-Ju;Cho, Sun-Ja;Lee, Sang-Joon
    • Journal of Environmental Science International
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    • v.15 no.10
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    • pp.983-988
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    • 2006
  • The textile remains have been affected largely by environmental factors including microorganisms because they were composed of organic compounds to be easy to damage. So, we selected 8 strains of the 131 isolated strains from museum environments and textile remains by high pretense activity, and identified them for measuring the antibacterial activity of Gingko biloba extracts. They were identified Genus Arthrobacter spp. 3 strains (Arthrobacter nicotiannae A12, Arthrobacter sp B12, Arthrobacter oxidans B13), Cenus Bacillus spp. 2 strains (Bacillus licheniformis D9, Bacillus cereus D33), Genus Pseudomonas spp. 2 strains (Pseudomonas putida A24, Pseufomonas fluorescene C21) and a Genus Staphylococcus sp. 1 strain (Staphylococcus pasteuri D3) as closest strains through the blast search of NCBI. Though antibacterial activity of the extracts of Gingko biloba leaves as MIC was lower than that of other pharmaceutical antibiotics. However the extracts was crude extracts, the extracts might have good antibacterial against most of the isolates from museum. Especially, the antifungal activity of Gingko biloba is known previously, the extracts of Gingko biloba leaves has possibility of usage as a good natural material for conservation of remains.

The Antioxidant and Anticancer Effects of Butterbur (Petasites japonicus) Extracts (머위(Petasites japonicus) 추출물의 항산화와 항암활성 효과)

  • Seo, Hun-Seok;Jeong, Bong-Hwan;Cho, Yong-Gu
    • Korean Journal of Plant Resources
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    • v.21 no.4
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    • pp.265-269
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    • 2008
  • The antioxidant activities of the extracts of butterbur (Petasites japonicus) derived from different extraction methods were investigated. SOD (superoxide dismutase)-like activity differed according to the extraction solvents, showing a greater antioxidant effect with ethanol solvent than that of water. Ethanol extracts of butterbur leaves showed higher SOD-like activity of 96.7% than those of water extracts. The contents of polyphenolic compounds were higher in water extracts than those in ethanol extracts. The highest content of polyphenolic compounds was 223mg/g dry weight for butterbur leaves. EDA of butterbur roots was 61.5% in the water extract and EDA in butterbur leaves was 34.9% in the ethanol extract. The anticancer effects with the extracts of butterbur were experimented by fractionations with different solvents. 41.9% of the growth of stomach cancer cells, SNU-719, were inhibited and also 72.7% of the growth of liver cancer cells, Hep3B, were inhibited by the butanol fractions of butterbur, while not affecting the growth of normal cell, DC 2.4.

New Antioxidant Sources; Tinged Autumnal Leaves of Maple and Cherry Trees

  • Lee, Jeong-Jae;Lee, Chung-Woo;Cho, Young-Ho;Park, Sung-Min;Lee, Bum-Chun;Pyo, Hyeong-Bae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.27 no.1
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    • pp.43-52
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    • 2001
  • Living systems constantly suffer from atmospheric reactive oxygen species and also produce inevitably them by the course of metabolism. Therefore, antioxidants play important roles in protecting the systems from various diseased caused by them. In this study, we investigated various tinged autumnal leaves as antioxidant sources. Among the, the red leaf extracts of Acer Palmatum THUNBERG.(Aceraceae: maple tree) and Prunus Donarioum Sieb. Var. spontanea Makino(Rosaceae : cherry trees) showed the highest anti-oxidativities. The major antioxidants in their red leaves were isolated and identified as vitexin from maple leaves and isoscutellarein-4'-O-$\beta$-glucopyranoside from cherry leaves. Finally, we evaluated the efficacy of skin care products containing the extracts by human use study. As a result, the tinged leaves of maple and cherry trees were evaluated as good antioxidant sources on the bases of antioxidativities, cytotoxicities, cell proliferation effects and human use study.

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