• Korean Journal of Breeding Science
• /
• v.40 no.1
• /
• pp.31-38
• /
• 2008

This experiment was carried out to improve the anther culture efficiency of barley (Hordeum vulgare L.). Callus induction rates from anther cultures of the five domestic naked barley and four unhulled varieties ranged from 0 to 5.6%, and plant regeneration rate to callus was 30.4% in the donor plants grown in a greenhouse during winter, among which the green plant regeneration rates ranged from 0 to 4.4%. Plant regeneration rate was 30.4% in the donor plants grown in a greenhouse during winter, whereas 21.3% in the normal field condition in spring. In addition, callus induction rates were 19.2% in plants grown in a normal field and 7.2% in drought-stressed condition, respectively. Being Considered the anther culture efficiency affected by the sampling time, the optimum sampling stage of anthers was 3~4 days before heading when the length between the 1st and 2nd auricles reaches 5 to 10 cm and at the uninucleate of pollen which the tip of the 2nd auricle aligns with the middle of panicle in the leaf sheath. Best callus induction rates came from the anthers stored at $4^{\circ}C$ for 3 weeks in a 10 to 15 cm diameter polyethylene bag with 5 to 10 panicles and Duwonchapssalbori and Saessalbori showed the higher induction rate of 4.8% and 1.7%, respectively.

• Korean Journal of Breeding Science
• /
• v.40 no.4
• /
• pp.512-516
• /
• 2008

A new naked oat cultivar 'Choyang' (Avena sativa L.) was developed by the breeding team at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2007. It was derived from a cross between 'Sikyonggwiri' and 'Gwiri23'. The cultivar, Sikyonggwiri is early heading while the breeding line Gwiri23 has a high yielding potential with large-size grain. Bulk method combined with pedigree selection program was employed in subsequent generations, and the promising line SO96025-B-303-44-2-5 was selected for agronomic performance in 2002. The line showed both high yield and good husking rate of seed in the Yield Trial tested at Suwon in 2003 to 2004, and was subsequently designated as 'Gwiri57'. Gwiri57 was evaluated for winter hardiness, earliness, and yield in four locations, Gimje, Iksan, Jeongeup and Jinju from 2005 to 2007 and was released as Choyang. Choyang headed 11 days earlier and matured 5 days earlier than the check cultivar 'Sunyang' in the paddy field condition. The new cultivar Choyang had 97 cm of culm length and 23.4 cm of spike length, 658 spikes per $m^2$, 75 grains per spike, 28.8 g of 1,000-grain weight, and 636 g of test weight. Choyang showed better winter hardiness than that of the check cultivar Sunyang, and similar seed quality to the check cultivar in respect to crude protein (12.9%) and ${\beta}$-glucan content (4.7%). However, it showed higher husking rate than the check cultivar. The grain yield of Choyang in the regional yield trial for 3 consecutive years was averaged $4.67MT\;ha^{-1}$, which was 38% higher than that of the check cultivar Sunyang. Choyang is recommended for the fall sowing cropping only in the southern area where daily minimum mean temperatures are averaged higher than $-4^{\circ}C$ in January, and is not recommended the in mountain area where frost damage is presumable.

• Korean Journal of Breeding Science
• /
• v.43 no.5
• /
• pp.413-421
• /
• 2011

The main objective of this study was to develop the multi-resistance lines to insects(brown planthopper; BPH, rice green leafhopper; GRH) and disease(blast; BL, bacterial blight; BB and rice stripe virus disease;RSV) with good grain quality and plant type by combining conventional breeding and marker assisted selection(MAS) and to eliminate the linkage drag effects between Bph1 gene and culm length, we conducted MAS of Bph1 gene in advanced backcross and double cross progenies. 'Nampyeong', 'Junam' and 'Milyang220' were used as the parent in this study. 'Milyang220' was used as the donor of brown planthopper resistance gene Bph1 with tall culm length. Two backcross progenies were developed using two recipients 'Nampyeong' carrying GRH resistance gene Grh3(t) with good grain appearance and 'Junam' harboring bacterial blight resistance gene Xa3 with short culm length. Two $BC_1$ generations were resulted from the backcrossing of the $F_1$ plants with recurrent parents 'Nampyeong' and 'Junam'. The second rounds of backcrossing($BC_2$) were derived from the cross of selected resistant $BC_1F_1$ plants based on heterozygous genotype of RM28493 linked to Bph1 gene. The double crossed population was constructed from the cross of between each heterozygous $BC_2F_1$ plants at RM28493 locus of '$Nampyeong^*3$ / Milyang220' and '$Junam^*3$ / Milyang220'., The homozygous alleles in Bph1 gene were selected using co-dominant DNA marker RM28493 in double crossed population. Eighty-five lines with multi-resistance to BL, BB, RSV, GRH and BPH were selected by bio-assay and MAS in generation of double crossing. The culm length, head rice ratio and yield of the selected multi resistance lines was ranged from 71 to 88 cm, from 51 to 93%, from 449 to 629 kg/10a. respectively. We can select a promising multi resistance line similar with 'Nampyeong' of major agronomic traits such as culm legnth, head rice ratio and yield. It was designated as Milyang265. Finally this study was developed the multi resistant varieties against to insects and diseases with the good grain quality 'Milyang265' by the advanced backcross and double cross combining MAS and it can be used as genetic resources of multi-resistance to insect and diseases in rice breeding programs.

• Korean Journal of Environmental Agriculture
• /
• v.37 no.4
• /
• pp.302-311
• /
• 2018

BACKGROUND: Dithianon (75%) formulation were mixed and sprayed as closely as possible by normal practice on the ten farms located in the Mungeong of South Korea. Patches, cotton gloves, socks, masks, and XAD-2 resin were used for measurement of the potential exposure of dithianon on the applicators wearing standardized whole-body outer and inner dosimeter (WBD). This study has been carried out to determine the dermal and inhalation exposure to dithianon during preparation of spray suspension and application with a power sprayer on a apple orchard. METHODS AND RESULTS: A personal air monitor equipped with an air pump, IOM sampler and cassette, and glass fiber filter was used for inhalation exposure. The field studies were carried out in a apple orchard. The temperature and relative humidity were monitored with a thermometer and a hygrometer. Wind speed was measured using a pocket weather meter. All mean field fortification recoveries were between 85.1% and 99.1% in the level of 100 LOQ (limit of quantification), while the LOQ for dithianon was $0.05{\mu}g/mL$ using HPLC-DAD. The exposure to dithianon on arms of the mixer/loader (0.0794 mg) was higher than other body parts (head, hands, upper body, or legs). The exposure to dithianon on the applicator's legs (3.78 mg) was highest in the body parts. The dermal exposures for mixer/loader and applicator were 10 and 8.10 mg, respectively, from a grape orchard. The inhalation exposure during application was estimated as 0.151 mg, and the ratio of inhalation exposure was 11.2% of the dermal exposure (inner clothes). CONCLUSION: The dermal and inhalation exposure on the applicator appeared to be 4.203 mg - 25.064 mg and $0.529{\mu}g-116.241{\mu}g$, respectively. The total exposures on the agricultural applicators were at the level of 2.596 mg - 25.069 mg to dithianon during treatment for apple orchard. The TER showed 3.421 (>1) when AOEL of dithianon was used as a reference dose for the purpose of risk assessment of the mixing/loading and application.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
• /
• v.14 no.1
• /
• pp.187-198
• /
• 2019

The purpose of this study is to seek ways of utilizing TLBMC by understanding business model of social ventures that are accompanied by social and environmental as well as economic missions. In order to achieve this, business models from economic, environmental and social perspectives will be analyzed, and we seek to enhance sustainability of social venture entrepreneurs. As the analysis tool, TLBMC(Triple Layered Business Model Canvas) expands upon the business model canvas that is widely utilized and recognizes economical terms. The TLBMC is proposed by Joyce, A., & Paquin, R. L.(2016) to help achieve a holistic view with horizontal and vertical associations. The study tries to overcome limitations of previous studies and observe a variety of economic, environmental, and social perspectives that social ventures should have with the TLBMC. As a result, it has the following implications; Firstly, creating separate social, environmental and economic business model canvas helps a business to have a holistic approach. Secondly, it was found that social venture characteristics of environmental and social perspectives were applied in practice. Lastly, we have established experience data on social venture business model. This study focuses on the opinions, the meanings and the subjective views of the participants. As a result, conclusions are drawn by the researchers ' assertions and has limitations as a research on case studies. However, this study will help people who are preparing or studying social ventures to have economic, environmental, and social perspectives. Also, redefinition of the direction and value of entrepreneurs operating social ventures, such as vision and mission, will help clarify the roles and responsibility of organizations. As a fundamental step for future empirical studies, this study could be the base for social venture business model studies.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.27 no.1
• /
• pp.39-48
• /
• 2019

Objective of this study was to evaluate an optimum biochar application rate and estimate the carbon sequestration based on the soil chemical properties and growth responses for biochar application during tomatoes cultivation. The treatments consisted of control as recommended application rates of fertilizers, 0.01%, 0.03%, 0.05%, and 0.07% of biochar application(w/w, biochar:soil). For effects of soil chemical properties, the $NO_3-N$contents in the soil were peaked at 9 days after transplanting. But there was not significant difference(p>0.05) among the treatments during cultivation periods. However, $NH_4-N$ contents in the biochar treatment were lower than the control until 14 days of transplanting. $P_2O_5$ contents in the biochar treatments were lower than that of the control until 19 days after transplanting except 0.01% of biochar application plot. $K_2O$ contents in soils treated with 0.01% and 0.03% of biochar were higher until 6 days after transplanting than that in the control. For N use efficiency of biochar application, it was observed that the 0.05% biochar application plot was highest among the treatments. The highest carbon sequestration was estimated at $2.83mg\;kg^{-1}$ for 0.03% of biochar application. However, it is considered that the optimum biochar application rate was 0.05% for tomato cultivation, considering the growth characteristics and yield components.

• Korean Journal of Environment and Ecology
• /
• v.33 no.1
• /
• pp.28-51
• /
• 2019

The purpose of this study was to analyze forest vegetation structure in Marugeum (the ridge line) area between Dakmokryeong and Daetjae in Baekdudaegan through vegetation classification, importance value, species diversity, and CCA using the data that were collected from 245 quadrates through Braun-Blanquet vegetation survey method from May to September in 2017. The results of the forest vegetation classification identified 8 vegetation units with Quercus mongolica community group at the highest level. Q. mongolica community group was classified into the Cornus controversa community, Buxus koreana community, Sasa borealis community, Abies nephrolepis community, and Q. mongolica typical community. C. controversa community was further classified into Quercus dentata group, Filipendula glaberrima group, Larix kaempferi group, and C. controversa typical group. The result of the important value analysis showed that Q. dentata had the highest importance value at 19.1% in vegetation unit 1 while Q. mongolica had the highest importance value at 22.7%, 38.3%, 25.6%, 41.3%, 27.9%, and 41.6% in vegetation unit 2, 4, 5, 6, 7, and 8, respectively, and L. kaempferi had the highest importance value at 27.6% in vegetation unit 3. As such, Q. mongolica species generally represented the communities of Marugeum (the ridge line) area of Dakmokryeong to Daetjae in Baekdudaegan. The results of species diversity showed that vegetation unit 1 and 2 were 3.305 and 3.236, respectively, which were relatively higher than other vegetation units. It was considered that this result was due to the influence of high emergence of present species. The results of the CCA analysis of the correlations between biotic environmental factors and vegetation types showed that vegetation unit 1 was mainly correlated with the megaphanerophyte ratio and vine plant ratio. In the correlations between abiotic environmental factors and vegetation types, vegetation unit 7 was significantly correlated with altitude. From the perspective of ecological management, vegetation unit 5 represented by B. koreana community was inhabited by a variety of plants due to the species composition and location environment due to the geological characteristics that are typical of limestone area. Vegetation unit 7 represented by A. nephrolepis community was typified as subalpine vegetation widely distributed by relict species and endemic species. We concluded that it is necessary to manage these vegetation units with an ecologically differentiated approach.

• Korean Journal of Poultry Science
• /
• v.45 no.4
• /
• pp.261-272
• /
• 2018

This study was conducted to evaluate the difference in the quality of chicken thigh meat from conventional and animal welfare farms during refrigeration storage over 9 days. Chicken thigh meat from conventional (CTC, n = 30) and animal welfare farms (CTW, n = 30) was tested. The pH value was significantly lower in CTW (6.28) than in CTC (6.37) on day 1; however, no significant differences were found on subsequent days. The yellowness of CTW was higher than that of CTC on day 1, but CTW showed lower yellowness than did CTC on day 7 and 9. The cooking loss, water holding capacity, lightness, redness, and coliform levels of CTC did not show any significant difference when compared with CTW during storage. The shear force of CTW was significantly higher than that of CTC on day 1, 3, 7, and 9. Total microorganism and coliform in CTC and CTW increased with increasing storage days. On day 7 and 9, the total microorganism level of CTW was lower than that of CTC. The thiobarbituric acid value of CTW was lower than that of CTC on day 9. The volatile basic nitrogen (VBN) of CTW was lower than that of CTC during storage. Anserine content and 1,1-diphenyl-2-picryl-hydrazyl(DPPH) scavenging activity of CTW was significantly higher than CTC on day 1. These results suggest that CTW stayed fresher for longer than did CTC because of low total microorganism level on day 7 and 9, and VBN during refrigerated storage.

• Journal of Wetlands Research
• /
• v.20 no.4
• /
• pp.295-303
• /
• 2018

Inland wetlands in the Republic of Korea provide key breeding and wintering habitats, while coastal wetlands provide nutrient-rich habitats for stopover sites for East Asia/Australasia Flyway(EAAF) migrants. However, since the 1960's, Korea has reclaimed these coastal wetlands gradually for agriculture and urban expansion. The habitat loss has rippled across global populations of migrant shorebirds in EAAF. To protect a similar loss, the United States, specifically Missouri, developed the moist-soil management technique. Wetland impoundments are constructed from levees with water-flow control gates with specific soils, topography, available water sources, and target goals. The impoundments are subjected to a combination of carefully timed and regulated flooding and drawdown regimes with occasional soil disturbance. This serves a dual purpose of removing undesirable vegetation, while maximizing habitat and forage for wildlife. Flooding and drawdown schedules must be dynamic with constantly shifting climate conditions. Korea's latitude ($N33^{\circ}25^{\prime}{\sim}N38^{\circ}37^{\prime}$) is comparable to Missouri ($N36^{\circ}69^{\prime}{\sim}N40^{\circ}41^{\prime}$); as such, moist-soil management could prove to be an effective wetland restoration technique for Korea. In order to meet specific conservation goals (i.e. shorebird staging site restoration), it is necessary to test the proposed methodology on a site that can meet the required specifications for moist-soil management. Moist-soil management has the potential to not only create key habitat for endangered wildlife, but also provide valuable ecosystem services, including water filtration.

• Korean journal of applied entomology
• /
• v.58 no.3
• /
• pp.183-187
• /
• 2019

To establish the cooperative monitoring network which can investigate introductions or outbreaks of high risk insect pests into Korea, seven universities, Gyeongsang National University, Kunsan National University, Seoul National University, Sunchon National University, Andong National University, Jeju National University, and Chungbuk National University, carried out seven regions' monitoring about nine high risk insect pests, Aceria diospyri, Bactrocera dorsalis, Bactrocera minax, Bactrocera tsuneonis, Cydia pomonella, Lobesia botrana, Proeulia sp., Solenopsis invicta, Stephanitis takeyai, from June to October in 2018. A total of 7,560 traps/visual scouting were investigated in 315 points of 105 local sites of seven regions, resulting the nine species, A. diospyri, B. dorsalis, B. minax, B. tsuneonis, C. pomonella, L. botrana, Proeulia sp., S. invicta, and S. takeyai, were not detected. From this study, we established the nationwide monitoring system which can early detect high risk insect pests and secured a bridgehead for monitoring invasive insect pests passing the border.