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Anther Culture Efficiency affected by Growth Condition and Pre-treatment Methods in Barley  

Park, Tae Il (Honam Agricultural Research Institute, NICS of RDA)
Kim, Young Jin (Honam Agricultural Research Institute, NICS of RDA)
Jeoung, Sun Ok (Honam Agricultural Research Institute, NICS of RDA)
Kim, Hyun Soon (International Technology Cooperation Center RDA)
Seo, Jae Hwan (Honam Agricultural Research Institute, NICS of RDA)
Yun, Song Joong (Division of Biological Resources Science, and Institute of Agricultural Science and Technology, Chonbuk National University)
Publication Information
Korean Journal of Breeding Science / v.40, no.1, 2008 , pp. 31-38 More about this Journal
Abstract
This experiment was carried out to improve the anther culture efficiency of barley (Hordeum vulgare L.). Callus induction rates from anther cultures of the five domestic naked barley and four unhulled varieties ranged from 0 to 5.6%, and plant regeneration rate to callus was 30.4% in the donor plants grown in a greenhouse during winter, among which the green plant regeneration rates ranged from 0 to 4.4%. Plant regeneration rate was 30.4% in the donor plants grown in a greenhouse during winter, whereas 21.3% in the normal field condition in spring. In addition, callus induction rates were 19.2% in plants grown in a normal field and 7.2% in drought-stressed condition, respectively. Being Considered the anther culture efficiency affected by the sampling time, the optimum sampling stage of anthers was 3~4 days before heading when the length between the 1st and 2nd auricles reaches 5 to 10 cm and at the uninucleate of pollen which the tip of the 2nd auricle aligns with the middle of panicle in the leaf sheath. Best callus induction rates came from the anthers stored at $4^{\circ}C$ for 3 weeks in a 10 to 15 cm diameter polyethylene bag with 5 to 10 panicles and Duwonchapssalbori and Saessalbori showed the higher induction rate of 4.8% and 1.7%, respectively.
Keywords
barley; anther culture; donor plant; pre-treatment; plant regeneration;
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