• The Korean Journal of Physiology and Pharmacology
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• 제1권1호
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• pp.45-53
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• 1997

In humans and many animal models with chronic progressive renal diseases, angiotensin-converting enzyme (ACE) inhibitor markedly attenuates the progression of nephropathy. Several studies have reported augmented gene expression and redistribution of renal renin in partial nephrectomized rats. Although precise mechanism(s) is not known, the renin-angiotensin system (RAS) may play an important role in the progression of renal diseases. Thus, this study was undertaken to examine the gene expression of renal renin, angiotensinogen, and $AT_1$ subtypes ($AT_{1A}$ and $AT_{1B}$) in rats with diabetic nephropathy, and the influences of lipopolysaccharide (LPS)-induced septicemia on the gene expression. Four weeks after streptozotocin (STZ) treatment (55 mg/kg, i.p.), rats were randomly divided into LPS-treated (1.6 mg/kg, i.p.) and control rats. At 6 hours after LPS treatment, the rats were killed and the kidney was removed from each rat. Northern blot and reverse transcription-polymerase chain reaction (RT-PCR)techniques were used to detect mRNA expression. STZ treatment markedly attenuated body weight gain and significantly increased blood glucose level. Renal renin content (RRC) was significantly decreased in the STZ-treated rats compared to that in control rats. The renal ACE activity between STZ-treated and control rats was not significantly different. Renal renin mRNA level was prominently increased, while angiotensinogen and $AT_{1A}$ mRNA levels were slightly decreased in STZ-treated rats compared to those in controls. $AT_1$B mRNA level did not differ in both groups. Acute LPS treatment did not show any significant changes of mRNA levels of intrarenal RAS components in both groups. These results suggest that intrarenal RAS components were differentially regulated in STZ-treated diabetic rats. Further studies are required to evaluate the relationship between intrarenal RAS and other vasomodulatory systems.

• Journal of Korean Medical classics
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• 제20권2호
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• pp.61-123
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• 2007

We translated 'poems of prograde' and 'poems of acupoints' which in the poems of acupoints and based on that, We considered the circulating direction and region of twelve meridian comparing the notion in common today with that in the 'poems of acupoints'. Finally, we got the conclusion like below 1. About circulating line of lung meridian, it'll be proper to correct that lung meridian passes through inner edge of biceps brachii muscle like heart and pericardium meridian. 2. On the part of kidney meridian in 'poems of prograde', it's right to be corrected as the circulating line that thru the inner line of vertebrae, enters heart and lung, and scattered tho breast. 3. 'Poems of prograde' can be separated two kinds go by starting-acupoints, ending- acupoints. First is 'poems of prograde' is begun from the end of members and ended in the body. Second is from Jungbu(中府) to Kimun(期門), circulating the members and the body with no ending. 4. On the above, we can find first 'poems of prograde' in "Chimkudaejeon(鍼灸大全)", "YangKyungjechimkujeonso(楊敬齋鍼灸全書)", "Chimkudaeseong(鍼灸大成)", also second can be found in "Neungmunjeonsudonginjihyul(凌門傳授銅人指穴)", "Chimkushinso(鍼灸神書)", "Kumchimbijeon(金針秘傳)", "Kyungrakhoepyun(經絡匯編), "Kyungrakko(經絡考)", "Jungsohoechamdongindosol(重西匯參銅人圖說)", "Kyungrakdoko(經脈圖考)", "ChimkuChuiyoung(針灸聚英)", "Shipsakyoungbalhwee(十四經發揮)", "Jakushimbopyokyul(刺灸心法要訣)", "Yukyoungdoik(類經圖翼)", "Yihakimmun(醫學入門)", etc. 5. Drawing an inference from above, the forms of "Jokbishibilmaek(足臂十一脈)" and "YumYangshibilmaek(陰陽十一脈)" are in "Mawangtoebaekso(馬王堆帛書)", are rather foreforms of circulation line that from the end of members and ended in the body than meridian prograde.

• The Journal of Pediatrics of Korean Medicine
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• 제16권1호
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• pp.149-169
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• 2002

This study was designed to observe the efficacy of Boyangsengjang-Tang(BST) on the growth of rats. The effects on growth, and the secretion of hormones in the serum was measure. Male BALA/C mice(weight 20-25g), and male ICR rats(weigh 120-150g), were each divided into 3 groups : Sample A, Sample B and a Control group. Each group consisted of 4 mice and 5 rats. Mice received $5{\mu}{\ell}$ BST, and rats received $50{\mu}{\ell}$ BST, daily. Sample A was administered with normal BST for 3 weeks. Sample B was was administered with 10 times the normal amount of BST for 3 weeks. Control group was administered with normal saline for 3 weeks. The body weight, body length, subcutaneous fat, length of femur, serum GH, serum IGFBP-3 and serum in TSH were measured at 1, 2, and 3 weeks. The results were as follows ; 1. The body weigh of the rats increased significantly in Sample A after 3 weeks when compared with control group. 2. The body length in rats increased significantly in Sample A when compared with control group. 3. The amount of subcutaneous fat in the mice increased significantly in Sample B after 1 week when compared with control group. The amount of subcutaneous fat in rats increased significantly in Sample A and Sample B after 3 weeks when compared with the control group. 4. The length of the femur in rats increased significantly in Sample in A and Sample B in 1, 2, and 3 weeks when compared with the control group. 5. The level of GH, IGFBP-3 and TSH in the serum was not statically different when compared with the control group. According to the above results, BST (which reinforces the Kidney's Yang and strengthens muscle and bone) increases body weight, body length, subcutaneous fat and the length of the femur when compaired with the control group. BST therefore seems to improve growth.

• Journal of Physiology & Pathology in Korean Medicine
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• 제31권3호
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• pp.173-181
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• 2017

The aim of this study was to identify the reliability and the validity of Five Organs Questionnaire (FOQ). In this study, 335 data was collected from early adulthood of A-city, D-city and C-city in South Korea from Dec. 2016 to Jan. 2017, and the collected data was analyzed with SPSS 23.0 software. The result showed that the Cronbach's ${\alpha}$ of five organs was 0.775-0.853. The reliabilities of test and retest Intra Correlation Coefficient (ICC) for the five organs were .891-.929 in pattern identification and .874-.930 in functional evaluation, respectively. Each organ was divided by two factors, and the factor loadings of Liver, Heart, Spleen, Lung and Kidney were 52.973%, 54.534%, 57.060%, 53.803%, and 46.337%, respectively. The functional evaluation of five organs was associated with self-rated health status as r was -.443 to -.583(p<.001), and quality of life as r was -.5.17 to -.716(p<.001), respectively. The test-retest diagnostic agreement was 67.2% and Kappa was 0.562. This study revealed that FOQ is a reliable and valid questionnaire.

• Asian-Australasian Journal of Animal Sciences
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• 제21권11호
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• pp.1673-1679
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• 2008

In order to study the biological function of gapdh gene in yak, and prove whether the gapdh gene was a useful intra-reference gene that can be given an important role in molecular biology research of yak, the cDNA sequence encoding glyceraldehyde-3-phosphate dehydrogenase from yak was cloned by the RT-PCR method using gene specific PCR primers. The sequence results indicated that the cloned cDNA fragment (1,008 bp) contained a 1,002 bp open reading frame, encoding 333 amino acids (AAs) with a molecular mass of 35.753 kDa. The deduced amino acids sequence showed a high level of sequence identity to Bos Taurus (99.70%), Xenopus laevis (94.29%), Homo sapiens (97.01%), Mus musculus (97.90%) and Sus scrofa (98.20%). The expression of yak's gapdh gene in heart, spleen, kidney and brain tissues was also detected; the results showed that the gapdh gene was expressed in all these tissues. Further analysis of yak GAPDH amino acid sequence implied that it contained a complete glyceraldehyde-3-phosphate dehydrogenase active site (ASCTTNCL) which ranged from 148 to 155 amino acid residues. It also contained two conserved domains, a NAD binding domain in its N-terminal and a complete catalytic domain of sugar transport in its C-terminal. The phylogenetic analysis showed that yak and Bos taurus were the closest species. The prediction of secondary structures indicated that GAPDH of yak had a similar secondary structure to other isolated GAPDH. The results of this study suggested that the gapdh gene of yak was similar to other species and could be used as the intra-reference to analyze the expression of other genes in yak.

• The Journal of Pediatrics of Korean Medicine
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• 제30권1호
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• pp.9-21
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• 2016

Objectives The purpose of this study is to analyze sinusitis patients who visited the department of pediatrics, OO Korean medicine hospital by using their age and to classify the clinical type by Korean medical theory. Methods The study was conducted based on 178 cases that consisted of sinusitis patients (from 1 to 15 years old) who visited OO Korean medicine hospital from March 2014 to March 2015. We analyzed the age of patients and classified them by the clinical type by reviewing patient's charts. After that, we compared the results with the results of previous studies. Results and Conclusions 178 Patients were studied. 2-years-age group was 19.7% of the study group which was higher than that of previous studies. The Lung-Kidney Yin Deficiency (肺腎陰虛) group was 33.1%, Wind-Heat (風熱) group was 29.8% and the Wind-Cold (風寒) group 18.5%, Spleen-Lung Qi Deficiency (脾肺氣虛) group was 15.7% and the Heart-Spleen Qi Deficiency (心脾氣虛) group was 2.8% of the study group. Children are full of Yang but lack of Yin (陽常有餘陰常不足) so they easily transform into heat and fire (化熱化火). The patients who had sinusitis were most likely to suffer from the common cold for more than a week, once a month. 45.1% of the people from the study group was suffered from common cold more than a week, and 43.8% of the people got common cold once a months. About 21.6 % and 18.3% of the people got common cold twice a month and once every 2 months, respectively. The remaining 15.7% got cold during the season changes.

• Korean Journal of Acupuncture
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• 제19권2호
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• pp.87-96
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• 2002

Objective : To study of Literature on the Ying Qi and Wei Qi One of thing that is among basis theory of Oriental medicine is Vital Essence, Vitality, Qi and Blood(精神氣血) justly. Results : Physiological special quality of Ying Qi(營氣) first, flows within Blood Circulation and the temper is mild and second, transform blood and grow up whole body, and follow through Pectoral Qi(宗氣). Wei Qi(衛氣) means running regeneration of material that do guard action of human body as refined nutritious substances transformed in food. Physiological special quality of Wei Qi(衛氣) first, nature is fast, smooth, and sharp. And it flows outside Blood Circulation. Second, go on muscslar gap without following through Pectoral Qi(宗氣). Ying blood(營血) flows within Blood Circulation means living body ingredient which thing reveals by circulation of the blood change phenomenon. Ying Qi(營氣) is Ying(營) in meaning which go on meridians, therefore meridians as wandering living body reaction route mean. This Ying Qi(營氣)'s physiology transform blood and grows up whole body and flows within Blood Circulation. It becomes most important ingredient of composition of blood, Wei Qi(衛氣) transforms and becomes nutrition constantly by running regeneration of material which transform in Jungcho(中焦) and spread by spreading action of upper chest exhaust in lower belly above the blader of apriority, Kidney-yang.

• The Journal of Korean Obstetrics and Gynecology
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• 제31권3호
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• pp.33-49
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• 2018

Objectives: The purpose of this study was to investigate the correlation between Pattern identification instrument and biomarkers in polycystic ovary syndrome patients. Methods: Pattern identification instrument questionnaire, Sasang constitutional test, body composition test, pulse analyzing test, laboratory test were performed and the results were analyzed. Correlation analysis was performed between pattern identification and laboratory test results. Results: Testosterone, SHBG, $17{\alpha}$-OH-progesterone, AMH were highest in the Kidney-Yang Deficiency (腎陽虛) group. LH, FSH, LH/FSH ratio were the highest in the Endogenous Heat due to Yin Deficiency (陰虛內熱) group. DHEA-S was the highest in the Dampness-Heat in Liver Meridian (肝經濕熱) group. E2, Prolactin, Cholesterol, Triglyceride, HDL-Cholesterol, LDL-Cholesterol, Glucose (FBS), Insulin, HOMA-IR ratio, HbA1c were the highest in the Phlegm-Dampness (痰濕) group. Conclusions: In this study, we obtained basic data analyzing the correlation between pattern identification instrument and biomarkers in polycystic ovary syndrome patients. If further studies are performed, we expect to be able to obtain clues to study the mechanism of polycystic ovary syndrome.

• Journal of Sasang Constitutional Medicine
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• 제21권2호
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• pp.27-41
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• 2009

1. Objectives : This research was proposed to find our the pathology of Soeumin in Sasang Constitutional Medicine(SCM). 2. Methods : The related contents of the pathology of Soeumin were selected in Je-Ma Lee's literatures such as "Dongmu-YuGo(東武遺稿)"(DYG), "Donguisusebowon-SaSangchobongyun(東醫壽世保元四象草本卷)"(DSS), "Donguisusebowon-GabObon(東醫壽世保元甲午本)"(DGO), "Donguisusebowon-ShinChukbon(東醫壽世保元辛丑本)"(DSC), and the research was written in order to find out the physiology and pathology of Soeumin in SCM. 3. Results and Conclusions : The chronical change of pathologic concept in Soeumin diseases as follows: Pathology in Soeumin diseases was much Cold Qi(寒氣), and more descending Qi, less ascending Qi in DYG, DSS. In "Discourse on the viscera and bowels" of DGO and DSC, Soeumin has a circulation of Water-Food Hot Qi of Spleen Group(脾黨) and Water-Food Cold Qi of Kidney Group(腎黨). Exterior Disease(表病) was the injury of Exterior-Qi such as eye-shoulder Qi(目膂氣) by Pleasure-Nature-Qi(樂性氣), and Interior Disease(裏病) was the injury of Interior-Qi such as spleen-stomach Qi(脾胃氣) by Joy-Emotion-Qi(喜情氣). All diseases of Soeumin are caused by insufficient Warm Yang Qi(陽煖之氣) in Spleen Group(脾黨), so the pathology of Soeumin was focused on Requisite energy(保命之主) and each small viscera and bowels(偏小之臟). In this viewpoint, the schema of Soeumin diseases such as Ulkwang-syndrome(鬱狂證), Mangyang-syndrome(亡陽證), Taeum-syndrome(太陰證) and Soeum-syndrome(少陰證) were designed to explain the mechanism of each syndrome.

• Journal of Acupuncture Research
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• 제31권1호
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• pp.51-60
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• 2014

Objectives : This study aimed to evaluate the effects of Alismatis Rhizoma Herbal-acupuncture( AR-HA) at $KI_{10}$(Umgok) on nephritis induced by lipopolysaccharide(LPS) in rat. Methods : Rats were injected with LPS to induce nephritis. The rats in $KI_{10}$-AR-HA group were treated with AR-HA, $KI_{10}$-NP group with needle prick, $KI_{10}$-saline group with normal saline injection respectively at $KI_{10}$ three times for a week. Several experimental items were performed including measurements of the numbers of RBC, WBC, neutrophil in blood, the levels of creatinine, $TNF-{\alpha}$, CINC-1 in serum, creatinine, total protein in urine, MPO in kidney. Results : Needle prick stimulation at $KI_{10}$ significantly reduced WBC in blood of LPS-stimulated rats. Saline injection at $KI_{10}$ significantly reduced WBC in blood, $TNF-{\alpha}$ in serum, total protein in urine of LPS-stimulated rats. AR-HA at $KI_{10}$ significantly reduced WBC, neutrophil in blood, $TNF-{\alpha}$, CINC-1 in serum, creatinine, total protein in urine of LPS-stimulated rats. Conclusion : AR-HA at $KI_{10}$ has a therapeutic effect on nephritis in LPS-stimulated rat. There may be a synergism between $KI_{10}$(Umgok) stimulation and AR-HA solution.