• Journal of Microbiology and Biotechnology
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• v.8 no.4
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• pp.369-377
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• 1998

Rotavirus is a major cause of severe gastroenteritis in young children and animals throughout the world. The VP7 of rotavirus is thought to induce the synthesis of neutralizing antibodies and to be responsible for determining viral serotypes. The cDNA coding for the VP7 capsid protein of human rotavirus, obtained from Korean patients (HRV-Y14), was cloned and its nucleotide sequence was determined. Comparative analysis of the nucleotide sequences between VP7 of Y14 and that of other foreign isolates showed $92.7~95.2\%$ homology to G1 serotypes (RV-4, KU, K8, WA), $74.2\%$ homolgy to G2 serotype HU-5, $76.4\%$ homology to G3 serotype SA-11, and $77.6\%$ homology to G4 serotype A01321. These data suggest that HRV-Y14 can be classified as a G1 serotype. cDNA coding for VP7 of HRV-YI4 was subcloned into the baculovirus vector and the VP7 glycoprotein was expressed in insect cells. The expressed proteins in Sf9 cell extract and tissue culture fluid were separated on SDS-PAGE, and Western blot analysis with monoclonal antibody raised against the synthetic peptide containing 21 amino acids within the VP7 conserved region was performed. The molecular weight of recombinant VP7 was estimated to be 36 kDa which is about the same size as the native VP7. Addition of tunicamycin in the culture media caused a reduction of the molecular weight of the recombinant VP7 indicating that the expressed protein was glycosylated.

• Journal of Life Science
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• v.28 no.4
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• pp.478-482
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• 2018

Human rotavirus is a causative agent of acute diarrhea among children. The artificial gene encoding the truncated $VP8^*$ protein of human rotavirus A (serotype 1 strain WA) was synthesized according to the Escherichia coli codon preference. The synthetic $VP8^*$ gene also possessed the NdeI and HindIII restriction sites for the convenient in-frame cloning for translation and a 6-histidine tag at C-terminus for Ni+ affinity purification. Molecular weight of the truncated $VP8^*$ protein deduced from the nucleotide sequences of the artificial gene was a 19.7-kDa. This synthetic $VP8^*$ DNA fragment was inserted into the pT7-7 expression vector and transformed into E. coli BL21 (DE3). Transformants harboring the synthetic gene encoding the $VP8^*$ protein was induced by supplement of a final concentration of 0.05 mM ITPG at $20^{\circ}C$. Protein crude extract from the E. coli transformants was subjected to Western blotting with the mouse anti-rotavirus capsid antibody, showing ~20-kDa $VP8^*$ protein band. The truncated $VP8^*$ protein band was also observed by Western blotting using the rabbit polyclonal antibody serum made against the truncated $VP8^*$ protein. This study suggested that the synthetic gene could be used as an easy way to produce the antigenic vaccine candidate for control of virus-associated diseases or to develop antibodies for diagnostic purpose.

• Journal of Radiation Protection and Research
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• v.40 no.2
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• pp.110-117
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• 2015

Recently, Due to the increased industry using radiation inspection equipment in the semiconductor, this demand of technology research is increasing. Although semiconductor inspection equipment is using low energy X-ray from 40 keV to 120 keV, Studies of radiation damage about the low energy X-ray are lacking circumstance in our country. Therefore, It is study that BJT (bipolar junction transistor) of one type of semiconductor elements are received radiation damage by low energy X-ray. BJT were used to the NXP semiconductor company's BC817-25 (NPN type), and Used the X-ray generator for the irradiation. Radiation damage of BJT was evaluated that confirm to analyse change of collector-emitter voltage of before and after X-ray irradiation when current gain fixed to 10. X-ray generator of tube voltage was setting 40 kVp, 60 kVp, 80 kVp, 100 kVp, 120 kVp and irradiation time was setting 180s, 360s, 540s into 180s intervals. As the result, We confirmed radiation damage in BJT by low energy X-ray under 120 keV energy, and Especially the biggest radiation damage was appeared at the 80 kVp. It is expected that ELDRS (enhanced low dose rate sensitivity) phenomenon occurs on the basis of 80 kVp. This studies expect to contribute effective dose administration of semiconductor inspection equipment using low energy X-ray, Also Research and Development of X-ray filter.

• Journal of radiological science and technology
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• v.37 no.2
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• pp.85-91
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• 2014

The purpose of this study is evaluated scatter radiation in digital radiological condition by using photo-stimulated luminescence (BaFBr:$Eu^{2+}$). Experiment condition changed kVp (from 50 kVp to 120 kVp), filed size (from $4{\times}4cm^2$ to $26{\times}26cm^2$) and phantom thickness (from 1 cm to 15 cm). This method was analysed ImageJ and characteristic curve of CR. This results was scatter radiation to primary radiation ratio increased from 50 kVp to 70 kVp, and it was fixed at over 80 kVp. The scatter radiation to primary radiation ratio are increased according to increasing the ratio of field size. Scatter radiation is also increased by increasing the phantom thickness.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2013.06a
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• pp.176-179
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• 2013

본 논문에서는 JCT-VC 에서 2013 년 1 월에 표준화가 완료된 High Efficiency Video Coding (HEVC)과 구글에서 2013 년 6 월에 개발 완료 예정인 VP9 의 압축 효율 비교를 수행한다. HEVC 는 UHD 등 고화질 방송 등에 대응하도록 디자인 되었으며, VP9 은 유튜브 (YouTube) 등과 같은 인터넷 비디오 스트리밍에 적합하도록 디자인되었다. VP9 의 경우 HEVC 와는 달리 로열티 프리 (royalty-free)를 지향하며 오픈소스 (open source) 방식으로 개발이 진행되고 있다. 본 논문에서는 HEVC 와 VP9 의 디자인 차별점을 소개하고, 랜덤 액세스 환경(Random Access, RA)과 저지연 환경 (Low Delay, LD)에서 HEVC 와 VP9 의 압축 효율을 비교한다. 실험 결과에 따르면, 방송 및 패키지 미디어 등에서 많이 사용될 랜덤 액세스 환경에서는 VP9 이 HEVC 대비 32.7% 열세를 보인다. 비디오 컨퍼런스등과 같은 저지연 환경에서는 VP9 이 HEVC 대비 26.7% 열세를 보인다. VP9 의 경우 개발이 완료된 것이 아니므로, 향후 압축 효율의 향상이 있을 것으로 기대된다.

• Journal of radiological science and technology
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• v.15 no.1
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• pp.89-97
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• 1992

X-ray quality is identified numerically by half value layer(H.V.L) and the HVL is affected by the kVp and the amount of filtration in the useful beam. X-ray quality evaluated by H.L.D is influenced by kVp and filtration. Author had several experiments with phantom in diameter of 8 cm normal adult chest, for reduction of radiation dose of the patients in diagnostic radiology and got some results. 1. H.V.L is increased the thicker the filter and the higher the kVp. 2. If the kVp is increased from 60 to 120, the skin dose can be reduced as 34%(Skin dose of 60 kVp with 4 mmAl filter : 100%). 3. If the 4 mmAl filter with 60 kVp is added to x-ray tube, skin dose can be reduced as 23% than no filter. 4. Therefore high kVp and filtration can increase output to input dose ratio and 120 kVp and 4 mmAl filter were most effective for reduction of patient dose in chest radiography.

• The Journal of the Korea Contents Association
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• v.10 no.6
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• pp.307-311
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• 2010

The aim of this study is to investigate PAE, as the result of the test of kVp accuracy, according to detector measurement method. Based on the indicated value of 70kVp, each distance between a focus and a kVp meter was 100cm, 80cm and 60cm and the angle of X-ray tube was set on $5^{\circ},\;10^{\circ},\;15^{\circ},\;20^{\circ},\;25^{\circ},\;30^{\circ}$. Each indicated value, 60kVp, 70kVp, 80kVp, 90kVp and 100 kVp, was used compare Small focus with Large focus. As a result, PAE on the side of cathode was higher than it on the side of anode in the case of 100cm and PAE on the side of anode was higher in the case of 80cm and 60cm. The coefficient rate was stable both the side of cathode and anode in the case of 100cm and it was fluctuated in the case of 80cm and 60cm. PAE in the case of Small focus was higher than Large focus and it was disproportionate to an indicated value. Error rate was in inverse proportion to the indicated value.

• Language and Information
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• v.20 no.1
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• pp.93-110
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• 2016

VP-ellipsis constructions in English can be schematized as S + [NP finite-AUX __ ] where the underlined part is understood to be a VP. Similarly, the pattern S + NP[-to] can be observed in Korean colloquial contexts. Though the English VP-ellipsis sentence pattern and the Korean pattern superficially seem to be similar, the Korean pattern exhibits peculiar properties: Syntactically, the NP of the pattern should have the delimiter -to. Semantically, it may convey ambiguous readings: VP-ellipsis-like and/or Stripping-like interpretation. To account for the pattern at issue, we propose a base-generated analysis driven by the delimiter -to within a construction grammar. We claim that the mother of the NP[-to] in this pattern is an S whose meaning is ambiguous between a VP-ellipsis-like and a Stripping-like reading. Consequently, the code of the VP-ellipsis in English is finite auxiliary verbs while that of the pattern S + NP[-to] in Korean is the delimiter -to.

• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.964-967
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• 2008

Two structural protein genes, VP19 and VP466, of white spot syndrome virus (WSSV) were cloned and expressed in Sf21 insect cells using a baculovirus expression system for the development of injection and oral feeding vaccines against WSSV for shrimps. The cumulative mortalities of the shrimps vaccinated by the injection of rVP19 and rVP466 at 15 days after the challenge with WSSV were 50.2% and 51.8%, respectively. For the vaccination by oral feeding of rVP19 and rVP466, the cumulative mortalities were 49.2% and 89.2%, respectively. These results show that protection against WSSV can be generated in the shrimp, using the viral structural protein as a protein vaccine.

• Journal of the Korean Society of Radiology
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• v.11 no.1
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• pp.63-68
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• 2017

To reduce the exposure dose in head CT, the use of low tube voltage is required. However, increasing noise may cause errors in the second data processing. In this study, we proposed a method to reduce noise by using low tube voltage. Experimental results show that the noise level is high at 100kVp and lowest at 140 kVp. The dose was lower at 100 kVp and higher at 140 kVp. As a result of applying the wavelet according to the threshold value, the noise value in the wavelet Th30 decreased to 4.51. Using the parameter condition(100 kVp, rotation time 0.5 sec, dose: 40.64 mGy) and the wavelet Th 30, the dose reduction of 65.3% was possible. We believe that applying the proposed method to head CT images will help to patient safety and interpret accurate information.