• International Journal of Industrial Entomology and Biomaterials
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• 제6권1호
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• pp.49-55
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• 2003

We describe the generation of transgenic silkworm that carrying the chimeric fibroin light chain (L-chain) gene. Previously, we have cloned the complete fibroin L-chain gene from the silkworm Baekok-Jam, Bombyx mori, and the complete fibroin gene from the oak silkworm, Antheraea yamamai. The 444 bp repetitive sequence of A. yamamai fibroin gene was inserted into the exon 6 of B. mori fibroin L-chain gene to produce chimeric fibroin L-chain gene. The chimeric fibroin L-chain gene was cloned into the polyhedrin gene site of Autographa californica nuclear polyhedrosis virus (AcNPV) to yield a recombinant baculovirus as a fibroin gene targeting vector, One-day-old fifth instar female silkworm larvae were injected with the recombinant baculovirus and then mated with normal male moths. Genomic DNA from their progenies was extracted and screened for the desired targeting event by using PCR and Southern blot analysis. The analysis showed that the chimeric fibroin gene had intergrated into the L-chain gene on the genome by homologous recombination and was transmitted through generations. The transgenic silkworm carrying the chimeric fibroin gene were approximately 43.2% in $F_2$ generation, and the silkworms synthesized the fusion protein in cocoons layer.

• International Journal of Industrial Entomology and Biomaterials
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• 제24권1호
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• pp.17-21
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• 2012

The effect of cultivation season on the cocoon filament quality of authorized silkworm variety, GoldenSilk was examined with cocoon filament character, reeling character, and fiber character during 3 years. In cocoon filament character, filament length of spring and autumn cocoon was 979 and 879 m, respectively. Cocoon filament weight of spring and autumn cocoon was 33.1 and 26.5 cg, which is lower than that of general bivoltine strain, BaeGokJam. Raw silk percentage of spring and autumn cocoon was 15.65 and 13.77%, respectively. In reeling character, reelability showed similar trend regardless of cultivation season and strain. However, the length and weight of non-broken filament of spring season were slightly higher than those of autumn cocoon. Cultivation season did not affect on the fiber characteristics such as clearness and neatness, lousiness, and degumming loss. In cocoon character, filament length, size, and weight, spring cocoon filament showed higher value than autumn cocoon, but lower than authorized general silkworm varieties. In reeling characteristics, non-broken length and weight showed the similar trends with the cocoon filament character. However, the reelability of GoldenSilk cocoon was not affected by cultivation season. The reelability of GoldenSilk cocoon was also similar with that of authorized general one. Fiber character including clearness and neatness, lousiness, and degumming loss was also similar regardless of the rearing season and different strain.

• 한국약용작물학회지
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• 제19권2호
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• pp.77-82
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• 2011

The antioxidative activity of various enzymatic extracts from Sarcodon aspratus (S. aspratus) was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. For this study, the S. aspratus were enzymatically hydrolyzed by seven carbohydrases (Viscozyme, Celluclast, Dextrozyme, AMG, Promozyme, Maltogenase, and Termamyl) and eight proteases (${\alpha}$-chymotrypsin, Alcalase, Flavourzyme, Neutrase, papain, pepsin, Protamax, and trypsin). The DPPH radical scavenging activities of Viscozyme and pepsin extracts were the highest, and the half maximal inhibitory concentration ($IC_{50}$) values were 0.896 and 0.734mg/mL, respectively. The Celluclast and trypsin extracts showed the highest scavenging activities on alkyl radical, and their $IC_{50}$ values were 0.278 and 0.575mg/mL, respectively. The Celluclast extracts was decreased cell apoptosis in PC-12 cells against $H_2O_2$-induced oxidative damage. The findings of the present study suggest that enzymatic extracts of S. aspratus exhibit antioxidative activity against oxidative stress on PC-12 cells.

• Asian-Australasian Journal of Animal Sciences
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• 제24권9호
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• pp.1303-1313
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• 2011

This study was conducted to provide the basic information to allow improved scientific assessment of velvet antler's quality by investigating the change of chemical composition during different antler growth stages in elk (Cervus elaphus canadensis). Twenty four antlers were harvested from elk stags (aged 4-5 years) on 65 days (VA65), 80 days (VA80) and 95 days (VA95) after button casting, and the chemical composition of each antler was determined in five sections (top, upper, middle, base, and bottom). Crude protein and ether extract content was the highest in the top section, whereas ash content was the highest in the bottom section in all groups (p<0.05). Glycosaminoglycan (GAG) content was higher in the VA65 group than in the VA95 group in the upper section of antler (p<0.05). The collagen content was higher in the VA65 group compared to the VA95 group in the middle and bottom sections (p<0.05), and increased downward from the top to the bottom section. The proportions of certain amino acids, including aspartic acid, glutamic acid and isoleucine were higher (p<0.05), whereas proline and glycine were lower in the top section of antler compared to all other sections (p<0.05). The proportion of linoleic acid, 11,14,17-eicosatrienoic acid, total ${\omega}$-3 and ${\omega}$-6 fatty acids and polyunsaturated fatty acids (PUFAs) for all sections in the VA65 group was higher than in the VA95 group (p<0.05). These results suggested that the quality of velvet antler is strongly influenced by antler development stage.

• Journal of Microbiology and Biotechnology
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• 제20권4호
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• pp.700-707
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• 2010

A novel antioxidative peptide (APVPH I, antioxidative peptides from venison protein hydrolysates I) was purified from venison by enzymatic hydrolysis, column chromatography of DEAE-Sephacel, and high-performance liquid chromatography. The molecular mass of the purified peptide was found to be 9,853 Da and the amino acid sequences of the purified peptide was Met-Gln-Ile-Phe-Val-Lys-Thr-Leu-Thr-Gly. The purpose of this study was to evaluate the effects of APVPH I against $H_2O_2$-induced neuronal cells damage in PC-12 cells. Antioxidative enzyme levels in cultured neuronal cells were increased in the presence of the peptide. In addition, APVPH I inhibited productions of nitric oxide (NO), reactive oxygen species (ROS), malondialdehyde (MDA), and cell death against $H_2O_2$-induced neuronal cell damage in PC-12 cells. It was presumed to be APVPH I involved in regulating the apoptosis-related gene expression in the cell environment. The present results indicate that APVPH I substantially contributes to antioxidative properties in neuronal cells.

• 한국건설관리학회논문집
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• 제7권6호
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• pp.79-89
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• 2006

종래의 깎기식 제거기에 의한 노면표시 제거작업은 인력에 의존하는 단순반복 작업으로 진행된다. 경제사회적인 측면에서 이러한 수작업은 매우 불리한데, 장시간 교통통제로 인하여 교통체증을 야기 시키기 때문이다. 이러한 교통체증은 도로 이용자의 불편과 혼잡비용이 증가시킬 뿐만 아니라 교통사고의 가능성도 가중시키게 된다. 본 연구의 목적은 워터젯 기술을 이용한 노면표시 제거 장비를 개발하고, 개발장비의 성능을 평가하는 것이다. 그 결과는 다음과 같다. 첫째, 워터젯을 이용한 노면표시 제거장비를 개발하여 현장실험한 결과, 기존방식과 비교하여 280.0%의 생산성 달성 비율을 얻을 수 있었다 또한 편익/비용 비율을 분석한 결과, 편익/비용 비율이 1보다 큰 3.28이 산출되었다. 따라서 노면표시제거 자동화 장비를 도입하는 경우 추가 소요되는 비용보다 발생되는 편익이 더 큼을 알 수 있다. 둘째, 개발장비로 기존 방식의 190일 작업물량을 작업할 경우, 장비 한대마다 연간 약 25.5억원의 교통혼잡비용을 절감할 수 있음을 알 수 있었다 따라서 노면표시제거 업체들이 본 연구의 개발장비를 도입함으로써 얻을 수 있는 사회적 편익은 막대할 것으로 기대된다.

• Spatial Information Research
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• 제18권3호
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• pp.85-94
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• 2010

본 연구는 승용차들의 요일제 준수여부 확인을 위한 RFID리더기들의 적정지점을 선정하는 방법을 제시하였다. 현재 전국의 3개의 주요 대도시에서 교통혼잡, 대기오염, 유류비 상승 등으로 인해 발생하는 문제를 해소하기 위하여 승용차요일제를 시행하고 있고, 앞으로 전국적으로 확대될 전망이다. 이를 위해서는 요일제 참여차량들의 요일제 준수여부확인이 면밀히 이루어져야 하고, 이를 통해 준수차량에 대해 보다 많은 혜택을 부여함으로써, 요일제를 확대 실시와 함께 실효성을 거둘 수 있을 것이다. 따라서, 본 연구는 수치지도와 지상사진 및 교통량자료를 통합 활용하여 현재 대구광역시에서 운영되고 있는 요일제용 RFID리더기들의 배치에 따른 확인구간과 우회시 확인이 불가능한 구간을 구체적화 하였고, 이를 토대로 확인이 불가능한 구간에 추가설치지점을 찾기 위해 도로상 설치가능한 구조물현황을 조사하고 도로망자료와 연계함으로써 추가설치시 적정지점을 선정하였다.

• 한국수산과학회지
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• 제34권5호
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• pp.556-562
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• 2001

본 연구에서는 비교적 성장이 빠르고 배양이 용이한 황갈편조 식물문 중 규조강에 속하는 P. tricornutum으로부터 선정하여 항 산화성 물질을 분리$\cdot$정제하여 물질의 구조를 밝혔다. P. tricornutum의 각 유기용매 추출물의 항산화성은 chloroform획분이 가장 좋았으며, 이 획분을 시료로 하여 silica gel column chromatography, PTLC 및 HPLC를 사용하여 항산화성 물질을 분리$\cdot$정제하였다. Silica gel column을 사용하여 분리된 획분의 항산화성은 dichloromethane: methanol (5: 1)로 용출시킨 획분에서 가장 높았으며, 이 획분의 PTLC 획분은 항산화성이 $20\%$ 증가하였다. 최종 정제된 물질의 $IC_50$값은 $8.3{\mu}g/mL$으로 화학적으로 합성된 항산화제인 BHT 및 BHA보다는 낮았지만 천연 항산화제인 $\alpha-tocopherol$보다는 다소 높게 나타났으며, 이 물질의 화학적 구조는 carotenoid류인 zeaxanthin으로 동정되었다.

• BMB Reports
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• 제34권3호
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• pp.219-224
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• 2001

To identify the antioxidative peptides in the gelatin hydrolysate of bovine skin, the gelatin was hydrolyzed with serial digestions in the order of Alcalase, pronase E, and collagenase using a three-step recycling membrane reactor. The second enzymatic hydrolysate (hydrolyzed with pronase E) was composed of peptides ranging from 1.5 to 4.5 kDa, and showed the highest antioxidative activity, as determined by the thiobarbituric acid method. Three different peptides were purified from the second hydrolysate using consecutive chromatographic methods. This included gel filtration on a Sephadex G-25 column, ion-exchange chromatography on a SP-Sephadex C-25 column, and high-performance liquid chromatography on an octadecylsilane chloride column. The isolated peptides were composed of 9 or 10 amino acid residues. They are: Gly-Glu-Hyp-Gly-Pro-Hyp-Gly-Ala-Hyp (PI), Gly-ProHyp-Gly-Pro-Hyp-Gly-Pro-Hyp-Gly (PII), and Gly-ProHyp-Gly-Pro-Hyp-Gly-Pro-Hyp (PIII), as characterized by Edman degradation and fast-atom bombardment mass spectrometry. The antioxidative activities of the purified peptides were measured using the thiobarbituric acid method, and the cell viability with a methylthiazol tetrazolium assay The results showed that PII had potent antioxidative activity on peroxidation of linoleic acid. Moreover, the cell viability of cultured liver cells was significantly enhanced by the addition of the peptide. These results suggest that the purified peptide, PII, from the gelatin hydrolysate of bovine skin is a natural antioxidant, which has potent antioxidative activity.

• 벤처창업연구
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• 제1권2호
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• pp.225-253
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• 2006

본 연구는 고속철도(Korea Train Express: KTX)의 지역간 교통수단으로의 역할과 기능을 충실히 하기 위해서 개선이 필요한 사항을 철도공사에 재직 중인 직원들을 대상으로 설문조사하여 분석하였다. 그 결과는 다음과 같다. 첫째, 건설교통부의 합리적인 지역간 교통배분 정책이 필요하다. 우리나라 중장거리권역 교통수단분담률에서 승용차와 고속철도의 비교우위 분기점은 대전과 대구 사이로 나타나고 있다. 고속철도의 경우 자동차 및 항공기와 경쟁구간인 서울${\sim}$대구 구간의 영업에 집중하고 단거리 구간에서의 경쟁은 가능한 피하는 것이 바람직 할 것으로 여겨진다. 둘째, KTX 경영개선을 통한 수요증대가 요구된다. 철도공사 직원들은 전문인력 양성과 전문인력 관리가 필요하다고 인식하고 있다. 그리고 고속철도의 운영정책은 운임결정에 대한 자율권 확보가 필요하다고 인식하고 있었다.