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http://dx.doi.org/10.4014/jmb.0909.09033

Neuroprotective Effects of a Novel Peptide Purified from Venison Protein  

Kim, Eun-Kyung (Department of Biotechnology, College of Medicine Konkuk University)
Lee, Seung-Jae (Department of Biotechnology College of Medicine Konkuk University)
Moon, Sang-Ho (Korean Nokyong Research Center, College of Medicine Konkuk University)
Jeon, Byong-Tae (Korean Nokyong Research Center, College of Medicine Konkuk University)
Kim, Bo-Kyung (Department of Physiology, College of Medicine Konkuk University)
Park, Tae-Kyu (Department of Biotechnology, College of Medicine Konkuk University)
Han, Ji-Sook (Department of Food Science and Nutrition, Pusan National University)
Park, Pyo-Jam (Department of Biotechnology, College of Medicine Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.4, 2010 , pp. 700-707 More about this Journal
Abstract
A novel antioxidative peptide (APVPH I, antioxidative peptides from venison protein hydrolysates I) was purified from venison by enzymatic hydrolysis, column chromatography of DEAE-Sephacel, and high-performance liquid chromatography. The molecular mass of the purified peptide was found to be 9,853 Da and the amino acid sequences of the purified peptide was Met-Gln-Ile-Phe-Val-Lys-Thr-Leu-Thr-Gly. The purpose of this study was to evaluate the effects of APVPH I against $H_2O_2$-induced neuronal cells damage in PC-12 cells. Antioxidative enzyme levels in cultured neuronal cells were increased in the presence of the peptide. In addition, APVPH I inhibited productions of nitric oxide (NO), reactive oxygen species (ROS), malondialdehyde (MDA), and cell death against $H_2O_2$-induced neuronal cell damage in PC-12 cells. It was presumed to be APVPH I involved in regulating the apoptosis-related gene expression in the cell environment. The present results indicate that APVPH I substantially contributes to antioxidative properties in neuronal cells.
Keywords
Venison; enzymatic hydrolysis; antioxidative peptide; neuronal cell; neuroprotective effects;
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