Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Free Radical Scavenging Activity and Protective Effect against H2O2-Induced Stress in Neuronal Cells of Enzymatic Extracts from Sarcodon aspratus

능이버섯 효소 추출물의 항산화 활성 및 H2O2로 유도된 스트레스에 대한 신경보호 효과

  • 이승재 (건국대학교 의료생명대학 생명공학) ;
  • 김은경 (건국대학교 의료생명대학 생명공학) ;
  • 오현정 (건국대학교 의료생명대학 생명공학) ;
  • 권혁주 (건국대학교 의료생명대학 생명공학) ;
  • 황진우 (건국대학교 의료생명대학 생명공학) ;
  • 문상호 (건국대학교 녹용연구센터) ;
  • 전병태 (건국대학교 녹용연구센터) ;
  • 박표잠 (건국대학교 의료생명대학 생명공학) ;
  • 임병우 (건국대학교 의료생명대학 응용생화학)
  • Received : 2020.12.07
  • Accepted : 2011.03.04
  • Published : 2011.04.30
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Abstract

The antioxidative activity of various enzymatic extracts from Sarcodon aspratus (S. aspratus) was evaluated by measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH), and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. For this study, the S. aspratus were enzymatically hydrolyzed by seven carbohydrases (Viscozyme, Celluclast, Dextrozyme, AMG, Promozyme, Maltogenase, and Termamyl) and eight proteases (${\alpha}$-chymotrypsin, Alcalase, Flavourzyme, Neutrase, papain, pepsin, Protamax, and trypsin). The DPPH radical scavenging activities of Viscozyme and pepsin extracts were the highest, and the half maximal inhibitory concentration ($IC_{50}$) values were 0.896 and 0.734mg/mL, respectively. The Celluclast and trypsin extracts showed the highest scavenging activities on alkyl radical, and their $IC_{50}$ values were 0.278 and 0.575mg/mL, respectively. The Celluclast extracts was decreased cell apoptosis in PC-12 cells against $H_2O_2$-induced oxidative damage. The findings of the present study suggest that enzymatic extracts of S. aspratus exhibit antioxidative activity against oxidative stress on PC-12 cells.

Keywords

References

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