• Natural Product Sciences
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• 제15권3호
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• pp.139-143
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• 2009

The anaphylactic allergic reaction is involved in many allergic diseases such as asthma and allergic rhinitis. In this report, I investigated the effect of ripe fruits of Rubus coreanus Miq. (Rosaceae) (RFRC) on the anaphylactic allergic reaction and studied its possible mechanisms of action. RFRC inhibited compound 48/80-induced systemic anaphylactic reaction in mice. RFRC dose-dependently decreased the IgE-mediated passive cutaneous anaphylaxis. In addition, RFRC decreased the gene expression and production of tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and interleukin (IL)-6 in phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187 (A23187)-stimulated human mast cells. These findings provide evidence that RFRC could be a candidate as an anti-allergic agent.

• Journal of Microbiology and Biotechnology
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• 제13권6호
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• pp.833-840
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• 2003

Sperm motility of salmonid fishes is suppressed by external $K^{+}$ and initiated by decrease of $K^{+}$ concentration surrounding the sperm. It was shown that the decrease in external $K^{+}$ concentration induced not only the initiation of sperm motility, but also hyperpolarization of the plasma membrane and synthesis of cAMP in the sperm of rainbow trout, steelhead trout, and masu salmon. Inhibitors of $K^{+}$ channels, especially voltage-dependent $K^{+}$ channels, inhibited these three reactions, and the inhibitions were abolished by subsequent addition of a $K^{+}$ ionophore, valinomycin, suggesting that $K^{+}$ efflux through the $K^{+}$ channel contributes to rapid changes in the membrane potential of sperm and cAMP synthesis, thereby resulting in the initiation of sperm motility of salmonid fishes.

• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2002년도 춘계학술발표대회 발표논문초록집
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• pp.6-6
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• 2002

This study was conducted to investigate the effect of recipient activation time on the nuclear remodeling, chromatin structure, pronuclear formation and in vitro development of bovine nuclear transfer embryos derived from adult ear skin cells. Somatic cells were transferred to enucleated oocytes after quiescent treatments by serum starvation or culture to confluency. Nuclear transfer embryos were activated with a combination of Ca/sup 2+/-ionophore and cycloheximide at 1, 1.5, 2, 2.5, 3, and 5 h after electrofusion. (omitted)

• Clinical and Experimental Reproductive Medicine
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• 제41권3호
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• pp.137-139
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• 2014

The effect of artificial oocyte activation (AOA) with a calcium ionophore on intracytoplasmic morphologically selected sperm injection (IMSI) was examined in patients with histories of repeated failed implantation attempts. Four singleton pregnancies and one twin pregnancy were obtained after embryos transfer (5/14, 35.7%). Therefore, AOA combined with IMSI can be considered an option for cycles with a fertilization defect and recurrent implantation failures.

• 대한한방소아과학회지
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• 제26권2호
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• pp.13-24
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• 2012

Objectives NJ is being used to treat inflammatory diseases in Korea. In this study, we attempted to evaluate the effects of NJ on atopic dermatitis (AD)-like lesions and mast cell-mediated allergy inflammation in vivo and in vitro. Methods and Results We investigated to ascertain the pharmacological effects of NJ on 2,4-dinitrofluorobenzene (DNFB)-induced allergic reactions under in vivo conditions. Additionally, to find possible explanations for the anti-inflammatory mechanisms of NJ, we evaluated the regulatory effects of NJ on the level of inflammatory mediators in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cells (HMC-1). Conclusions NJ inhibited the production of the inflammatory cytokines (IgE, IL-6, IL-8 and TNF-${\alpha}$) significantly in vivo and in vitro.

• Natural Product Sciences
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• 제10권1호
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• pp.24-28
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• 2004

The effects of aqueous extract of Artemisia iwayomogi (Compositae) (AIAE) on the mast cell-dependent allergic and inflammatory reactions were investigated. AIAE (0.05 to 1 g/kg) dose-dependently inhibited systemic allergic reaction induced by compound 48/80 in mice. AIAE (0.1 and 1 g/kg) also significantly inhibited local allergic reaction activated by anti-DNP IgE. AIAE (0.001 to 1 mg/ml) dose-dependently inhibited the histamine release from rat peritoneal mast cells (RPMC) activated by compound 48/80. Moreover, AIAE inhibited the secretion of interleukin (IL)-6 in phorbol 12-myristate 13-acetate (PMA) plus calcium ionophore A23187-stimulated human mast cell line (HMC-1) cells. These results provide evidence that AIAE may be beneficial in the treatment of allergic diseases.

• Bulletin of the Korean Chemical Society
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• 제27권8호
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• pp.1127-1132
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• 2006

A new salicylate-selective electrode based on the complex N,N'-1,4-butylene bis(3-methyl salicylidene iminato) copper(II) as the membrane carrier was developed. The electrode exhibits a good Nernstian slope of 59.1 ${\pm}$ 1.0 mV/decade and a linear range of $1.0\;{\times}\;10^{-6}$-1.0 M for salicylate. The limit of detection was $5.0\;{\times}\;10^{-7}$ M. It has a fast response time 10 s and can be used for more than three months. The selective coefficients were determined by the fixed interference method (FIM) and could be used in the pH range 4.5-10.5. It was employed as an indicator electrode for direct determination of salicylate in pharmaceutical samples.

• Asian-Australasian Journal of Animal Sciences
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• 제3권3호
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• pp.183-186
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• 1990

Monensin administered as a slow release capsule to Droughtmaster steers grazing mixed pastures containing Stylosanthes hamata or grass pastures fertilized with N, had no effect on growth rate over 111 day period. Monensin significantly increased the level of propionic acid (p<0.001) and decreased the level of butyric acid (p<0.01) in the rumen. The lack of response to monesin was partly attributed to the poor pasture conditions and growth rate of the steers during part of the experimental period. An implant of oestradiol improved growth rates during the period of poor forage quality and in the subsequent 56 days when pastures were of high quality following rain. Mean growth rates over the entire 157 days for control, monensin and monensin/oestradiol treatments were 0.37, 0.37 and 0.50 kg/d respectively. It was concluded that when pasture conditions are sufficient only for the maintenance of liveweight, production can be improved by an oestradiol implant but not by feeding an ionophore such as monensin.

• Asian-Australasian Journal of Animal Sciences
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• 제15권8호
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• pp.1091-1097
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• 2002

Capacitated goat spermatozoa generated diacyl glycerol (DAG) when suspended in Krebs-Ringer bicarbonate medium and induced by progesterone or $Ca^{2+}$ ionophore A23187. We have added Sn-1-oleoyl-2-acetyl glycerol externally, to study the effect of DAG in goat sperm acrosomal exocytosis. Addition of neomycin abolished the DAG generating capacity of progesterone in a dose dependent manner, suggesting the involvement of a phosphoinositidase C activated phospholipase C system in the process. The level of increase in phosphatidic acid was considerably low and was produced well after the DAG generation thereby suggesting the involvement of a DAG kinase which phosphorylates DAG to produce PA. The inhibition of progesterone mediated effect by inhibitors of $GABA_A/Cl^{-}$ channel and $Ca^{2+}$ channels further supports the evidence that the events of binding of agonist to the receptor(s), opening of $Ca^{2+}$ channels and the activation of phospholipase C are reconciled to perform the function of acrosome reaction in capacitated goat spermatozoa.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 1998년도 제4차 학술발표대회 및 정기총회
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• pp.45-47
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• 1998

STZ를 주사한 후 혈액내 인슐린 및 포도당 농도는 당뇨병 유발에 의해 반비레적인 관계를 보였다. 대조군의 부정소두와 체내 정자의 농도 그리고 부정소미내 정자의 수는 당뇨병 유발에 의한 혈당량의 변화에 민감하게 감소하였다. 자발적 첨체반응율은 대조군의 부정소미와 수정관내 두부위간 유의한 차이로 증가하였다. 3일군과 14일군의 부정소미내 정자의 자발적 첨체반응율은 대조군에 비해 유의하게 증가하였다. ARIC test 결과 대조군, 3일군, 그리고 14일군에서 약 12%의 차이를 보였다. 결과저긍로 당뇨병 유발에 의해 부정소미내 정자수의 감소와 자발적 첨체반응율이 상대적으로 높아지나 ARIC test의 결과에서 부정소미내 정자는 수정능력이 있는 것으로 판단된다. 따라서 당뇨병은 정자의 수적인 감소를 유발하며 부정소내 기능의 이상을 초래하여 정자성숙을 방해하여 자발적 첨체반응을 증가시킴으로서 개체의 수정율 감소를 초래할 수 있는 것으로 사료된다.