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http://dx.doi.org/10.5713/ajas.2002.1091

Role of Diacyl Glycerol (DAG) in Caprine Sperm Acrosomal Exocytosis Induced by Progesterone  

Somanath, P.R. (Animal Biochemistry Division, National Dairy Research Institute)
Gandhi, K.K. (Animal Biochemistry Division, National Dairy Research Institute)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.15, no.8, 2002 , pp. 1091-1097 More about this Journal
Abstract
Capacitated goat spermatozoa generated diacyl glycerol (DAG) when suspended in Krebs-Ringer bicarbonate medium and induced by progesterone or $Ca^{2+}$ ionophore A23187. We have added Sn-1-oleoyl-2-acetyl glycerol externally, to study the effect of DAG in goat sperm acrosomal exocytosis. Addition of neomycin abolished the DAG generating capacity of progesterone in a dose dependent manner, suggesting the involvement of a phosphoinositidase C activated phospholipase C system in the process. The level of increase in phosphatidic acid was considerably low and was produced well after the DAG generation thereby suggesting the involvement of a DAG kinase which phosphorylates DAG to produce PA. The inhibition of progesterone mediated effect by inhibitors of $GABA_A/Cl^{-}$ channel and $Ca^{2+}$ channels further supports the evidence that the events of binding of agonist to the receptor(s), opening of $Ca^{2+}$ channels and the activation of phospholipase C are reconciled to perform the function of acrosome reaction in capacitated goat spermatozoa.
Keywords
Goat; Spermatozoa; Acrosome Reaction; Diacyl Glycerol; Phospholipase C;
Citations & Related Records
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