• Journal of Microbiology and Biotechnology
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• v.31 no.10
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• pp.1455-1464
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• 2021

Trehalose is a non-reducing disaccharide in increasing demand for applications in food, nutraceutical, and pharmaceutical industries. Single-step trehalose production by trehalose synthase (TreS) using maltose as a starting material is a promising alternative process for industrial application due to its simplicity and cost advantage. Pseudomonas monteilii TBRC 1196 was identified using the developed screening method as a potent strain for TreS production. The TreS gene from P. monteilii TBRC 1196 was first cloned and expressed in Escherichia coli. Purified recombinant trehalose synthase (PmTreS) had a molecular weight of 76 kDa and showed optimal pH and temperature at 9.0 and 40℃, respectively. The enzyme exhibited >90% residual activity under mesophilic condition under a broad pH range of 7-10 for 6 h. Maximum trehalose yield by PmTreS was 68.1% with low yield of glucose (4%) as a byproduct under optimal conditions, equivalent to productivity of 4.5 g/l/h using enzyme loading of 2 mg/g substrate and high concentration maltose solution (100 g/l) in a lab-scale bioreactor. The enzyme represents a potent biocatalyst for energy-saving trehalose production with potential for inhibiting microbial contamination by alkaline condition.

• Journal of Engineering Education Research
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• v.21 no.3
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• pp.56-65
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• 2018

With rapid advance of technologies including information and communication technologies, jobs are evolving faster than ever. Architectural engineering is no exception in this regard, and the green architectural engineering is emerging fast as a promising new field. In this study, a Delphi study of expert architectural engineers are conducted to find out (1) near future prospects of the field, (2) near future emerging jobs, (3) competencies needed for these jobs, and (4) educational content necessary to build these competencies with regards to the green architectural engineering. Initial Delphi survey consisting of open-ended questions in the above four areas were conducted and came out with 65 items after duplicate removal and semantic refinements. Further refinements via second and third wave of Delphi results into 40 items that the 13 architectural engineering experts may largely agree upon as future prospects with regards to the green architectural engineering. Findings indicate that it is expected that the demand for green architectural engineering and needs for automatic energy control system increase. Also, collaborations with other fields is becoming more and more important in green architectural engineering. The professional work management skills such as knowledge convergence, problem solving, collaboration skills, and creativity linking components from various related areas seem to also be on the increasing need. Near future ready critical skills are found to be the building environment control techniques (thermal, light, sound, and air), the data processing techniques like data mining, energy monitoring, and the control and utilization of environmental analysis software. Experts also agree on new curriculum for green building architecture to be developed with more of converging subjects across disciplines for future ready professional skills and experiences. Major topics to be covered in the near future includes building environment studies, building energy management, energy reduction systems, indoor air quality, global environment and natural phenomena, and machinery and electrical facility. Architectural engineering community should be concerned with building up the competencies identified in this Delphi preparing for fast advancing future.

• Food Science and Industry
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• v.51 no.4
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• pp.270-277
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• 2018

The rising global demand for food and beverages with higher protein content provides manufacturers with great opportunities for innovation and premium positioning of their products as healthy choices. However, the market price volatility and supply risks associated with animal-based proteins can quickly erode margins and profitability. A diversified protein strategy that includes plant-based soy protein greatly improves your ability to predict profitability over time, while maintaining or even improving product quality.

• Journal of Engineering Education Research
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• v.24 no.1
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• pp.15-23
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• 2021

The first job of university graduates is the beginning of career development, and it has a great influence on a personal life in the transition process of a labor market later. The study has compared and analyzed the effects of major variables (whether or not one participates in the career and employment programs, the satisfaction in the education infrastructure and curriculum) related to university education that determine the satisfaction in the first job of graduates from the entire university of 4-year general courses and the engineering college with experiences of having the first job. Through this, it is meaningful to make it possible for the design of university education related to career and employment tailored to the engineering college. The results of 2017 Graduate Occupational Mobility Survey were used as the data for analysis, which was analyzed by the decision tree analysis. As it was found that the most important factor determining the satisfaction in the first job was student welfare facilities for the entire graduates among education infrastructure and was major curriculum and its content among education curriculum for the graduates from the engineering college, it was analyzed that factors related to majors were more important compared to other majors in the engineering college. The customized major curriculum and content should be considered as a priority, taking into account of the demand of the industry for the successful settlement of graduates from the engineering college in a labor market.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.12
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• pp.612-618
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• 2020

In recent years, the demand for external window cleaning has increased, but the process is a very dangerous manpower-dependent operation. In addition, it is difficult to clean at the desired frequency in a business that values cleanliness. Therefore, there is a need to automate this work. This paper presents the concept of a device that can be attached to a specific window and clean the window continuously. The economic feasibility of this device was analyzed. The estimated manufacturing cost of the equipment was approximately 10 million won, but the possible investment cost was at least 9.8 million won for five years of endurance and 103 million won for 10 years of endurance. Therefore, the expected savings well exceed the equipment cost, and it was evaluated as having economic feasibility. Since this study analyzed only quantitative indicators, the expected cost reduction due to a reduction in safety accidents, productivity improvement, construction time reduction, and quality improvement was not considered. Therefore, it is expected that the calculated economic feasibility will be more accurate if the cost reduction effect by the automation equipment is calculated by adding the expected values not considered in this study.

• Journal of Korea Water Resources Association
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• v.55 no.1
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• pp.11-21
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• 2022

The COVID-19 started to spread at early 2020 in South Korea and has been threatening our life in many aspects. Countermeasures such as social distancing to prevent COVID-19 spread have brought many changes in our society an human life. In this study, as a part of the COVID-19 pandemic management, drinking water usage change is analyzed to evaluate potential risks on water supply service. We collected hourly water use data of residential area in S city, which is a mid-size city in South Korea, before and after the COVID-19 pandemic. The collected data were analyzed to reveal the changes in total water consumption, water usage weight, and hourly water-demand pattern caused by the COVID-19 pandemic. The case study revealed the noticeable changes in water consumption caused by the COVID-19 pandemic and required more secured and adaptive operation of drinking water system under the pandemic situation caused by infectious disease.

• Journal of Information Technology Services
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• v.20 no.5
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• pp.89-104
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• 2021

Food delivery service combined with IT technology and HMR (Home Meal Replacement) are rapidly growing due to the COVID-19. Recently, the demand for salads along with HMR has increased among office workers in their 20s and 30s who are interested in health and beauty. Freshcode is a food startup with 6 years of experience that started selling salad products through O2O service. Freshcode applied for a patent for a service that collects orders from nearby areas and delivers them on the same day to a designated delivery address 'FCOSPOT' to save shipping costs. In March 2021, in recognition of the growth potential of the regular delivery service, Freshcode received an investment of 6 billion won in Series A. This study may have practical implications to early-stage startups and scale-up stage startups through a longitudinal case study on the growth of a single company. As for the research method, the lean startup methodology and lean canvas were used in the early stage of startup. In particular, the process of the build-measure and learn feedback-loop, which is the core of lean startup methodology, was applied to each major decision-making step. In the scale-up stage after 5 years, the business model canvas was used to schematize the growth as a food online O2O platform to verify continuous innovation. This case study has three main findings. First, the idea of 'FCOSPOT' was successfully implemented through the Lean Startup methodology. Second, Freshcode demonstrated the scalability of the differentiated business model of shared base delivery O2O. Third, a key factor of success was the digital integrated communication operation strategy that maximizes the experience for the created customers.

• Korean small business review
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• v.43 no.4
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• pp.155-172
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• 2021

This research means that it analyzed the conflict structure of key stakeholders in the operation and management of the Regulation Free Zone. The analysis results are as follows. First, for the safety review related to the amendment of the law, the process of presenting a preliminary review opinion is additionally required. Second, institutional improvement is needed for the complicated preliminary preparation stage in the management system issue. Third, there is the issue of whether to provide an incentive structure to the local innovation ecosystem or to leave it to the autonomy of the participating companies and guarantee the choice in the post-management issue. In the future, this research suggests a method to systematically prepare a micro-policy demand identification process through continuous monitoring using the Q method.

• Journal of Powder Materials
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• v.29 no.2
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• pp.159-165
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• 2022

Rare earth elements, which are important components of motors, are in high demand and thus constantly get more expensive. This tendency is driven by the growth of the electric vehicle market, as well as environmental issues associated with rare-earth metal manufacturing. TC 298 of the ISO manages standardization in the areas of rare-earth recycling, measurement, and sustainability. Korea, a resource-poor country, is working on international standardization projects that focus on recycling and encouraging the domestic adoption of international standards. ITU-T has previously issued recommendations regarding the recycling of rare-earth metals from e-waste. ISO TC 298 expands on the previous recommendations and standards for promoting the recycling industry. Recycling-related rare earth standards and drafts covered by ISO TC 298, as well as Korea's strategies, are reviewed and discussed in this article.

• Design Convergence Study
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• v.16 no.5
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• pp.125-140
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• 2017

This study explores potential capability of a machine learning approach for monitoring urban morphology based on an evident case study. The case study conveys year 2006 investigations on interpreting urban morphology of Boston Main Streets by applying a machine learning approach. From the lesson of the precedent study, in 2016, another field research and interview was conducted to compare changes in urban situation, data commons culture, and technology innovation during the decade. This paper describes open possibilities to advance urban monitoring for morphological changes. Most of all, a multi-participatory data platform enables managing urban data system in real time. Second, collaboration with machines with artificial intelligence can intervene the framework of the urban management system as well as transform it through new demands of innovative industries. Recently, urban regeneration became a dominant urban planning strategy in Korean, therefore, urban monitoring is on demand. It is timely important to correspond to in-situ problems based on empirical research.