• 생명과학회지
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• 제17권10호
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• pp.1336-1340
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• 2007

히아신스 Pink Pearl 품종의 인편배양에서 자구의 재생과 생장은 1.0 mg/l와 3.0 mg/l의 농도에서 IAA보다 IBA가 더 효과적이었으며, 절편체의 기부가 배지에 삽식되는 정상적인 치상 방향이 역방향의 치상 방법에 비해 자구의 재생과 뿌리의 생장에 더 효율적인 것으로 나타났다. 계대배양에서 재생된 자구와 뿌리의 생장은 한천배지보다 펄라이트가 첨가된 액체배지에서 증가되었다. 이와 같은 히아신스 Pink Pearl 품종의 기내배양의 경우 자구 재생을 위한 초대배양은 한천 고체배지를, 재생 된 자구의 생장은 펄라이트의 액체배지를 사용하는 2 단계의 교대배양 시스템이 더 유용함을 시사한다.

• 한국자원식물학회지
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• 제22권5호
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• pp.394-402
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• 2009

기내배양을 이용하여 Pteris cretica 'Wilsonii'의 효율적인 대량번식법을 개발하기 위한 목적으로 근경, 엽신 및 엽병조직으로부터 식물체 재분화를 유도하였다. 그 결과, 균질화된 근경의 절편에서만 신초가 재생되었다. 식물체 재분화를 유도하는데 적합한 배지는 1%의 sucrose와 $50\;mg{\cdot}mL^{-1}$의 $NaH_2PO_4$를 첨가한 1/8MS배지였다. 근경 절편으로부터 식물체의 재생을 촉진하기 위하여 생장조절물질이 첨가된 배지에서 2달 동안 절편을 배양한 후, 생장조절물질을 첨가하지 않은 배지로 이식한 결과, $1{\mu}M$의 kinetin과 $5{\mu}M$의 NAA를 혼용첨가한 처리구에서 신초의 재생이 가장 왕성하였다. BA 첨가구에서는 분열조직의 일종인 GGB가 다량 유도되었으나, 형성된 GGB에서 신초 재생율은 낮은 것으로 나타났다. MS배지의 적정 무기물 및 비타민의 농도를 구명하는 실험을 마친 뒤, 형성된 유묘를 이용하여 기외순화 시킨 결과, 1/8MS배지에서 형성된 유묘가 생존율이 가장 높았으며, 생육 또한 왕성하였다.

• Journal of Forest and Environmental Science
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• 제27권2호
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• pp.61-72
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• 2011

Micropropagation is an alternative mean of propagation that can be employed in mass multiplication of plants in relatively shorter time. Recent modern techniques of propagation have been developed which could facilitate large scale production of true-to-type plants and for the improvement of the species using genetic engineering techniques in the next century. An overview on the in vitro propagation via meristem culture, regeneration via organogenesis and somatic embryogenesis is presented. The usefulness of the plants in commercial industry as well as propagation techniques, screening for various useful characteristics and the influence of different cultural conditions in the multiplication, rooting and acclimatization phases on the growth of tissue cultured plant discussed.

• 한국작물학회지
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• 제37권5호
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• pp.419-424
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• 1992

콩에서의 조직배양 가능성을 알아보고자 콩 유묘를 기내에서 배양시킨 뒤, 여러 부위를 채취하여 각기 다른 배지 치상한 결과는 다음과 같다. 1. 줄기정단조직 배양에서는 대부분 shoot가 분화되어 완전한 식물체를 얻을 수 있었다. 2. 자엽절 조직배양에서는 multiple shoots가 나타나 대량증식에 유용했으나 배지에 따른 영향이 컸다. 3. 배축 및 상배축 조직배양에서는 callus만 분화되엇다. 4. 엽조직배양에서는 callus 분화 후 계대배양했을 때 shoot가 분화되었으나 완전한 식물체를 얻기는 힘들었다.

• 한국자원식물학회지
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• 제32권1호
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• pp.19-28
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• 2019

An efficient shoot regeneration condition for pea cv. 'Sparkle' was developed by using optimum explant, plant growth regulator concentrations, and pretreatment of BA onto explant. The average shoot number per explant showed the highest on two kinds of shoot induction media (MSB5 media containing 2 mg/L BA and a combination of 2 mg/L BA and 1 mg/L TDZ) when cotyledonary node explants were cultured. Moreover, the pretreatment of explant in 200 mg/L BA solution was found to be more effective in shoot induction than that of non-pretreatment. By histological study, cell division and proto-meristem were formed near the surface of the sub-epidermal and epidermal cell layers of cotyledonary node in earlier than 3 days after culture. The analysis of genetic stability of regenerants by using thirteen ISSR markers showed that in vitro regenerated plants showed polymorphism with 8.3% compared with their mother plants.

• Journal of Plant Biotechnology
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• 제36권3호
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• pp.236-243
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• 2009

The halophyte Leymus chinensis (Trin.) is a perennial rhizome grass (tribe Gramineae) that is widely distributed throughout China, Mongolia and Siberia. This study was conducted to investigate an optimal condition for plant regeneration from mature seeds, leaf base segments, and root segments in L. chinensis. Plant growth regulators affecting embryogenic callus induction and plant regeneration were investigated by four-factor-three-level [L9 (34)] orthogonal test in this study. The effects of explants types (mature seeds, leaf base segments and root segments), callus types, medium types were examined in this study. Wild type (WT) and Jisheng No. 1 plants (JS) were used for primary callus induction. A clear explants difference was seen during callus induction; mature seeds were considered as the preferred explants; and the highest frequency of callus induction was obtained in Medium 6 using mature seeds as explants in WT. Plant regeneration ability was evaluated by frequencies of green callus forming, shooting, rooting, and shooting with roots. Effect of α-naphthalene acetic acid (NAA) on shoot regeneration was remarkable with the highest frequency of 70.8% in WT after 2-month culture. The medium with 0.2- 0.5 mg/L NAA was found to have the highest shoot induction. All regenerated shoots were successfully rooted when transferred on half-strength Murashige and Skoog (MS) basal medium. The acclimatized plantlets were grown to mature with flowering and seeds setting in green house conditions.

• Journal of Plant Biotechnology
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• 제43권4호
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• pp.438-443
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• 2016

The in vitro propagation of the commercially important Phalaeonopsis hybrid 'Little gem' was achieved by culturing the apical part and axillary buds excised from flower stalks. The explants were cultured on 5 different basal media: $3.0{\cdot}L^{-1}$ Hyponex and $4.0{\cdot}L^{-1}$ peptone ($H_3P_4$) and Murashige & Skoog (MS) media were shown to be suitable for shoot regeneration. The MS medium supplemented with $5.0mg{\cdot}L^{-1}$ 6-benzylaminopurine (BA) was found to be more efficient for shoot regeneration. However, the number of shoots induced by axillary buds was higher than that induced by the apical part. Incubation of the apical part under darkness for one week, as well as of the explants in the same medium with activated charcoal (AC) $0.5g{\cdot}L^{-1}$ promoted shoot regeneration and shoot growth; similar growth was not observed with axillary buds.

• 한국자원식물학회지
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• 제17권1호
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• pp.43-47
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• 2004

기내에서 삽목 배양중인 한라구절초[( Chrysanthemum zawadskii ssp. coreanum(Nakai) Y.N. Lee)]의 잎으로부터 효과적인 식물체 재생을 위한 NAA와 BA농도 및 배지고형물의 효과를 조사하였다. 0.2% Gelite를 고형물로 하였을 때 한라구절초의 잎 재분화에는 BA농도보다는 NAA 농도가 더 영향을 미쳤으며, NAA 농도가 1 mg/L 이상이면 모든 조직 에서 재분화를 보였다. 신초수는 NAA 단용 처리에서 많은 경향이었으며 NAA 1 mg/L 처리시가 가장 양호하였다. 배지고형물로 agar과 gelite를 가지고 재분화 효과를 조사한 결과, gelite 첨가시 잎절편 당 신초수가 많았으며 agar 첨가시에는 잎절편당 신초수가 적은 반면에 신초의 길이가 길게 나타났다. 한라구절초의 잎절편으로부터 재분화에는 MS에 3% sucrose, 0.8% agar와 NAA 1 mg/L를 첨가하여 25 $^{\circ}C$, 2,000 lux 정도에서 배양하는 것이 효과적이었다.

• Plant Resources
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• 제4권3호
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• pp.111-120
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• 2001

The efficiency of in vitro regeneration of four clones of Populus euramericana, Canada blanc, Eugenii, I-45/51, and Wisconsin #5, was examined. Cytokinins and the combinations with auxins affected the rate of regeneration from the explants of root segments, stem internodes, and leaf discs. Overall, BA and the combination with auxins were effective in root segments and leaf discs of the Canada blanc clone, whereas zeatin and the combination with auxins were important in stem internodes of the Wisconsin #5 clone. The highest number of shoots averaging 17.6 $\pm$ 0.47 from root segments in the Canada blanc clone,18.2 $\pm$ 3.0 from stem internodes in the Wisconsin #5 clone, and 17.8 $\pm$ 1.92 from leaf discs in the Canada blanc clone were obtained with 2.0 mg/1 BA, 2.0 mg/l zeatin combined with 0.2 mg/l IAA, and 0.5 mg/l BA combined with 0.05 mg/l 2,4-D, respectively. In particular, the addition of 2,4-D into cytokinin medium promoted shoot proliferation.

• 식물조직배양학회지
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• 제24권3호
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• pp.125-128
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• 1997

'Gala' 잎조직의 신초 재분화에 효과적인 cytokinin 종류와 농도는 BA 4.0 ㎎/L였고, auxin 종류와 농도는 IAA, IBA, 및 NAA 각각 0.1 ㎎/L였다. BA와 NAA를 농도별로 조합 처리하여 'Gala'의 잎, 절간 및 엽병의 신초 재분화 정도를 비교한 결과, 잎조직은 0.5 ㎎/L NAA + 6.0 ㎎/L BA와 0.5㎎/L NAA + 0.8 ㎎/L BA 처리구에서, 절간조직은 0.1 ㎎/L NAA + 0.8 ㎎/L BA와 1.0 ㎎/L NAA + 0.8 ㎎/L BA 처리구에서 신초 재분화율이 가장 높게 나타났으며, 엽병조직은 0.1 ㎎/L NAA + 4.0 ㎎/L BA 처리구에서 가장 높았다.