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http://dx.doi.org/10.5352/JLS.2007.17.10.1336

Efficient Bulblet Regeneration and Growth from Bulb Scale of Hyacinthus orientalis L. cv. Pink Pearl Cultured in vitro  

Lee, Kyung-Soon (Department of Plant Biotechnology, Dong-A University)
Chung, Chung-Han (Department of Biotechnology, Dong-A University)
Chung, Yong-Mo (Floricultural Experiment Station, Gyeongnam ARES)
Kim, Doh-Hoon (Department of Genetic Engineering, Dong-A University)
Jeong, Soon-Jae (Department of Plant Biotechnology, Dong-A University)
Nam, Jae-Sung (Department of Plant Biotechnology, Dong-A University)
Kim, Gyung-Tae (Department of Plant Biotechnology, Dong-A University)
Yi, Young-Byung (Department of Plant Biotechnology, Dong-A University)
Publication Information
Journal of Life Science / v.17, no.10, 2007 , pp. 1336-1340 More about this Journal
Abstract
The regeneration and growth of bulblets from the bulb scale segments of Hyacinthus orientalis L. cv. Pink Pearl were more efficient in IBA than IAA at the same concentrations (1.0 and 3.0 mg/l). The normal (base-down) orientation of explants was more effective for bulblet regeneration and root growth than the inverted (base-up) orientation. The growth of bulblets and roots was increased higher in the perlite than the agar medium. These results suggested that the alternate culture system, first cultured in the agar medium for bulblet regeneration, and then in the perlite medium for bulblet growth, may be more useful for efficient in vitro culture of hyacinth (H. orientalils) cv. Pink Pearl.
Keywords
Bulblet regeneration and growth; explant orientation; Hyacinthus orientalis; IBA; perlite;
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