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http://dx.doi.org/10.7732/kjpr.2019.32.1.019

Optimization of Shoot Induction, Histological Study and Genetic Stability of in vitro Cultured Pisum sativum cv. 'Sparkle'  

Kantayos, Vipada (School of Plant Production Science, Sunchon National University)
Bae, Chang-Hyu (Department of Well-being Resources, Sunchon National University)
Publication Information
Korean Journal of Plant Resources / v.32, no.1, 2019 , pp. 19-28 More about this Journal
Abstract
An efficient shoot regeneration condition for pea cv. 'Sparkle' was developed by using optimum explant, plant growth regulator concentrations, and pretreatment of BA onto explant. The average shoot number per explant showed the highest on two kinds of shoot induction media (MSB5 media containing 2 mg/L BA and a combination of 2 mg/L BA and 1 mg/L TDZ) when cotyledonary node explants were cultured. Moreover, the pretreatment of explant in 200 mg/L BA solution was found to be more effective in shoot induction than that of non-pretreatment. By histological study, cell division and proto-meristem were formed near the surface of the sub-epidermal and epidermal cell layers of cotyledonary node in earlier than 3 days after culture. The analysis of genetic stability of regenerants by using thirteen ISSR markers showed that in vitro regenerated plants showed polymorphism with 8.3% compared with their mother plants.
Keywords
Cotyledonary node; Genetic Stability; Histological study; Pea; Shoot Regeneration;
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1 Aftabi, M., A.T. Nagawo and F. Hassan. 2018. Improved protocol for Agrobacterium-mediated transformation of pea (Pisum sativum). Mol. Biol. 7(1), doi:10.4172/2168-9547.1000202   DOI
2 Ai-Qurainy, F., M. Nadeem, S. Khan, S. Alansi, M. Tarroum, A.A. Al-Ameri, A.-R. Z. Gaafar and A. Alshameri. 2018. Rapid plant regeneration, validation of genetic integrity by ISSR markers and conservation of Reseda pentagyna an endemic plant growing in Saudi Arabia. Saudi J. Biol. Sci. 25:111-116.   DOI
3 Azade, L.K., A.S. Indieka, M.O. Adero, S. Kiboi and N.O. Amugune. 2016. In vitro regeneration of pigeon pea using leaf explants. Afr. Crop Sci. J. 24(2):191-201.   DOI
4 Benson, E.E. 2000. Special symposium: In vitro plant recalcitrance: An introduction. In Vitro Cell Dev. Biol. Plant 36:141-148.   DOI
5 Bohmer, P., B. Meyer and H.J. Jacobson. 1995. Thidiazuron induced high-frequency of shoot induction and plant regeneration in protoplast derived pea callus. Plant Cell Rep. 15(1-2):26-29.   DOI
6 Brown, D.C.W. and T.A. Thorpe. 1986. Plant regeneration by organogenesis: In Cell Culture and Somatic Cell Genetics of Plants, Vasil, I.K. (ed.), Academic Press, New York, USA. 3:49-65.
7 Cao, X. and F.A. Hammerschlag. 2002. A two-step pretreatment significantly enchance shoot organogenesis from leaf explant from highbush blueberry cultivar bluecrop. Hort. Sci. 37(5): 819-821.   DOI
8 Dibax, R., G.B. Alcantara, M.P. Machado, J.C.B. Filho and R.A. Oliveira. 2013. Protocol optimization and histological analysis of in vitro plant regeneration of RB92579 and RB93509 sugarcane cultivars. Ciencia Rural, Santa Maria 43(1):49-54.
9 Fatima, N. and M. Anis. 2012. Role of growth regulators on in vitro regeneration and histological analysis in Indian ginseng (Withania somnifera L.) Dunal. Physiol. Mol. Biol. Plants 18(1):59-67.   DOI
10 Goto, S., R.C. Thakur and K. Ishii. 1998. Determination of genetic stability in long-term micropropagated shoots of Pinus thunbergii Parl. using RAPD markers. Plant Cell Rep. 18:193-197.   DOI
11 Huang, W.J., G.G. Ning, G.F. Liu and M.Z. Bao. 2009. Determination of genetic stability of long-term micropropagated plantlets of Platanus acerifolia using ISSR markers. Biol. Plant. 53(1):159-163.   DOI
12 Jackson, J.A. and S.L.A. Hobbs. 1990. Rapid multiple shoot production from cotyledonary node explants of pea (Pisum sativum L.). In Vitro Cell Dev. Biol. Plant 26:825-835.
13 Kantayos, V. 2019. Characterization of plantlet regeneration and genetic transformation of legume plants. School of Plant Production Science, Ph.D. Thesis, Sunchon National Univ., Korea (in Korea).
14 Kim, D-g., V. Kantayos, D.-K. Kim, H.-G. Park, H.-H. Kim, E.-S. Rha, S.-C. Lee and C.-H. Bae. 2016. Plant regeneration by in vitro tissue culture in Korean soybean (Glycine max L.). Korean J. Plant Res. 29:143-153.   DOI
15 Klu, G.Y.P. 1996. Efforts to accelerate domestication of winged bean (Psophocarpus tetragonolobus (L.) DC.) by means of induced mutations and tissue culture. Thesis Dissertation of Department Laboratory of Plant Breeding, Wageningen University and Research. Netherlands.
16 Mallo'n, R., R.O. Juan and M.L. Gonza'lez. 2010. In vitro propagation of the endangered plant Centaure aultreiae: assessment of genetic stability by cytological studies, flow cytometry and RAPD analysis. Plant Cell Tiss. Org. Cult. 101:31-39.   DOI
17 Mariappan, M., S.K. Thiruppathi and V.R. Mandali. 2016. Organogenesis and evaluation of genetic homogeneity through SCoT and ISSR markers in Heliteres idora L., a medicinally important tree. S. Afr. J. Bot. 106:204-210.   DOI
18 Nayak, S.A., S. Kumar, K. Satapathy, A. Moharana, B. Behera, D.P. Barik, L. Acharya, P.K. Mohapatra, P.K. Jenaand and S.K. Naik. 2012. In vitro plant regeneration from cotyledonary nodes of Withania somnifera (L.) Dunal and assessment of clonal fidelity using RAPD and ISSR markers. Acta Physiol. Plant. 35(1):195-203.   DOI
19 Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497.   DOI
20 Nayak, S., T. Kaur, S. Mohanty, G. Ghosh, R. Choudhury, L. Acharya and E. Subudhi. 2011. In vitro and ex vitro evaluation of long-term microparpagated turmeric as analyzed through cytophotometry, phytoconsituents, biochemical and molecular markers. Plant Growth Regul. 64:91-98.   DOI
21 Raveendar, S., A. Premkumar, S. Sasikumar, S. Ignacimuthu and P. Agastian. 2009. Development of a rapid, highly efficient system of organogenesis in cowpea Vigna unguiculata (L.) Walp. S. Afri. J. Bot. 75:17-21.   DOI
22 Reinhardt, D., E.-R. Pesce, P. Stieger, T. Mandel, K. Baltensperger, M. Bennett, J. Traas, J. Friml and C. Kuhlemeier. 2003. Regulation of phyllotaxis by polar auxin transport. Nature 426:255-260.   DOI
23 Sainger, M., D. Chaudhary, S. Dahiya, R. Jaiwal and P.K. Jaiwal. 2015. Development of an efficient in vitro plant regeneration system amenable to Agrobacterium- mediated transformation of a recalcitrant grain legume blackgram (Vigna mungo L. Hepper). Physiol. Mol. Biol. Plants 21(4): 505-517.   DOI
24 Schroeder, H.E., A.H. Schotz, T. Wardley-Richardson, D. Spencer and T.J.V. Higgins. 1993. Transformation and regeneration of two cultivar of pea (Pisum sativum). Mol. Biol. Gene Reg. 101:751-757.
25 Tie, M., Q. Luo, Y. Zhu and H. Li. 2013. Effect of 6-BA on the plant regeneration via organogenesis from cotyledonary node of cowpea (Vigna unguiculata L. Walp). J. Agr. Sci. 5(5), doi:10.5539/jas.v5n5p1   DOI
26 Shah, S.H., S. Ali, S.A. Jan, J. Din and G.M. Ali. 2015. Callus induction, in vitro shoot regeneration and hairy root formation by the the assessment of various plant growth regulators in tomato (Solanum lycopersicum Mill.). J. Ani. Plant Sci. 25(2):528-538.
27 Swartz, H.J., R. Bors, F. Mohamed and S.K. Naess. 1990. The effect of in vitro pretreatments on subsequent shoot organogenesis from exicised Rubus and Malus leaves. Plant Cell Tiss. Org. Cult. 21:179-184.   DOI
28 Thomas, T.D. 2007. Pretreatment in thidiazuron improves the in vitro shoot induction from leaves in Curculigo orchioides Gaertn., an endangered medicinal plant. Acta Physiol. Plant. 29:455-461.   DOI
29 Tzitzikas, E.N., M. Bergervoet, K. Raemakers, J-P. Vincken, A. Lammeren and R.G.F. Visser. 2004. Regeneration of pea (Pisum sativum L.) by a cyclic organogenic system. Plant Cell Rep. 23:453-460.   DOI
30 Tran, T.H., T.V. Bui and T.Y. Feng. 2016. Role of plant growth regulators on shoot development of shoot apical meristems of banana genotypes. Acta Hort. 1114:211-218.   DOI
31 Veltcheva, M., D. Svetleva and S. Petkova. 2003. In vitro cultivation and regeneration of bean (Phaseolus vulgaris L.). Biotechnol. Biotechnol. Equip. 17(1):50-58.   DOI
32 Wang, H., M. Li, Y. Yang, J. Dong and W. Jin. 2015. Histological and endogenous plant growth regulators changes associated with adventitious shoot regeneration from in vitro leaf explants of strawberry (Fragaria ${\times}$ ananassa cv. 'Honeoye'). Plant Cell Tiss. Organ Cult. 23(3):479-488.
33 Yi, J-Y., K. Balaraju, H.-J. Baek, M.-S. Yoon, H.-H. Kim and Y.-Y Lee. 2018. Cryopreservation of Citrus limon (L.) Burm. F shoot tips using a droplet-vitrification method. Korean J. Plant Res. 31(6):684-694.   DOI
34 Werner, E.T., T.C.B. Soares, A.B.P.L. Gontijo, J.D. Souza Neto and J.A.T. Amaral. 2015. Genetic stability of micropropagated plants of Crambe abyssinica Hochst using ISSR markers. Gene Mol. Res. 14 (4):16450-16460.   DOI
35 Woo, S.M. and H.Y. Wetzstein. 2008. Morphological and histological evaluations of in vitro regeneration in Elliottia racemosa leaf explants induced on media with thidiazuron. J. Am. Soc. Hort. Sci. 133(2):167-172.   DOI
36 Yancheva, S., D. Svetleva, Sp. Petkova and A. Atanassov. 1999. Regeneration possibilities of Bulgarian bean cultivars Plovdiv 11M and Dobrudjanski 7. Biotechnol. Biotechnol. Equip. 13(1):40-43.   DOI
37 Zhihui, S., M. Tzitzikas, K. Raemakers, M. Zhengqiang and R. Visser. 2009. Effect of TDZ on plant regeneration from mature seeds in pea (Pisum sativum). In Vitro Cell Dev. Biol. Plant 45(6):776-782.   DOI