Browse > Article

Medium Composition Affecting In Vitro Plant Regeneration and Acclimation of Pteris cretica 'Wilsonii'  

Shin, So-Lim (Dept. of Horticultural Science, Chungbuk National University)
Lee, Cheol-Hee (Dept. of Horticultural Science, Chungbuk National University)
Publication Information
Korean Journal of Plant Resources / v.22, no.5, 2009 , pp. 394-402 More about this Journal
Abstract
Adventitious shoots were induced from pinnae, petiole and rhizome in Pteris cretica 'Wilsonii' in order to develop the efficient mass propagation method, using in vitro culture. Only homogenized rhizome segments could regenerate young sporophytes. Efficient regeneration of multiple shoots was obtained on the one-eighth strength MS medium containing 1% sucrose, and $50mg{\cdot}L^{-1}$ $NaH_2PO_4$. To achieve higher rate of regeneration from rhizome segments, rhizome segments were exposed to growth regulators for 2 months and then subcultured on hormone-free medium. The greatest shoot regeneration was obtained by $1{\mu}M$ kinetin with $5{\mu}M$ NAA. BA was effective in formation of GGB (kind of meristems), but they showed low shoot regeneration rate. Plants obtained from present experiments were transplanted to examine good environmental conditions for acclimation. Juvenile plants obtained by the one-eighth strength MS medium showed highest survival rate and vigorous growth at the seedling stage.
Keywords
fern$NaH_2PO_4$; plant growth regulator; sporophyte; tissue culture;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Bertrand, A.M., M.A, Albuerne, H. Fernandez, A. Gonzalez, and R. Sanchez-Tames. 1999. In vitro organogenesis of Polypodium cambricum. Plant Cell Tiss. Org. Cult. 57:65-69   DOI   ScienceOn
2 Fernandez, H., A.M. Bertrand, and R. Sanchez-Tames. 1996a. Influence of tissue culture conditions on apogamy in Dryopteris affinis sp. affinis. Plant Cell Tiss. Org. Cult. 45:93-97   DOI   ScienceOn
3 Fernandez, H., A.M. Bertrand, and R. Sachez-Tames. 1996b. Micropropagation and phase change in Blechnum spicant and Pteris ensiformis. Plant Cell Tiss. Org. Cult. 44:261-265   DOI   ScienceOn
4 Garcia, E. and L. Furelli. 1987. Clonal mass propagation of the fern Cyrtomium falcatum. Acta Hort. (ISHS) 212:655-660
5 Higuchi, H. and W. Amaki. 1989. Effect of 6-benzylaminopurine on the organogenesis of Asplenium nidus L. through in vitro propagation. Sci. Hort. 37:351-359   DOI   ScienceOn
6 Lee, Y.H. 2007. Masspropagation of Korea green native ferns in Vitro for developing materials of interior landscape architecture. J. Kor. Soc. People plants Environ. 10:65-73. (in Korean)
7 Shin, S.L., J.K. Hwang, and C.H. Lee. 2009. Medium composition affecting In Vitro masspropagation and morphogenesis in prothalli of Pteris cretica 'Wilsonii'. Flower Res. J. 17:114-120. (in Korean)
8 Teng, W.L. 1997. Activated charcoal affects morphogenesis and enhances sporophyte regeneration during leaf cell suspension culture of Platycerium bifurcatum. Plant Cell Rep. 17:77-83   DOI   ScienceOn
9 Thakur, R.C., Y. Hosoi, and K. Ishii. 1998. Rapid in vitro propagation of Matteuccia struthiopteris (L.) Todaro - an edible fern. Plant Cell Rep. 18:203-208   DOI   ScienceOn
10 Sheffield, E. and S. Laird. 1986. Antheridia and archegonia of the apogamous fern Pteris cretica. Ann. Bot. 57:139-143   DOI
11 Teng. W.L. and M.C. Teng. 1997. In vitro regeneration patterns of Platycerium bifurcatum leaf suspension culture. Plant Cell Rep. 16:820-824   DOI   ScienceOn
12 Raghavan, V. 1989. Developmental biology of fern gametophytes. Cambridge Univ. Press. New York
13 Ambrozic-Dolinsek J., M. Camloh, B. Bohanec, and J. Zel. 2002. Apospory in leaf culture of staghorn fern (Platycerium bifurcatum). Plant Cell Rep. 20:791-796   DOI   ScienceOn
14 Kwa, S.H., Y.C. Wee, and C.S. Loh. 1991. Production of aposporous gametophytes and calli from Pteris vittata L. pinnae strips cultured in vitro. Plant Cell Rep. 10:392-393   DOI   ScienceOn
15 Higuchi, H., W. Amaki, and S. Suzuki. 1987. In vitro propagation of Nephrolepis cordifolia Prsel. Sci. Hort. 32:105-113   DOI   ScienceOn
16 Fernandez, H., A.M. Bertrand, and R. Sanchez-Tames. 1993. In vitro regeneration of Asplenium nidus L. from gametophytic and sporophytic tissue. Sci. Hort. 56:71-77   DOI   ScienceOn
17 Salome, M., S. Pais, and M. Casal. 1987. Propagation of the fern Adiantum capillus-veneris through tissue culture of the circinate part of young leaves. Acta Hort. (ISHS) 212:651-654
18 Jeong, J.A. and C.H. Lee. 2006. Effects of medium composition on plant regeneration in Asplenium incisum Thunb. J. Kor. Flower Res. Soc. 14:176-185. (in Korean)
19 Jones, D.L. 1987. Encyclopaedia of ferns. Timber press, North America
20 Lee, S.H. and C.T. Yu. 2002. Effect of genotype and explant on somatic embryogenesis and acclimatization of Acanthopanax senticosus. Kor. J. Med. Crop Sci. 10:217-221. (in Korean)   과학기술학회마을
21 Fernandez, H. and M.A. Revilla. 2003. In vitro culture of ornamental ferns. Plant Cell Tiss. Org. Cult. 73:1-13   DOI   ScienceOn
22 Paek, K.Y., H.C. Lee, J.K. Choi, and B.H. Kwack. 1984. Masspropagation Nephrolepis exaltata by runner tips in vitro. J. Kor. Soc. Hort. Sci. 25:313-321. (in Korean)
23 Camloh, M. and N. Gogala. 1992. In vitro culture of Platycerium bifurcatum gametophytes. Sci. Hort. 51:343-346   DOI   ScienceOn
24 Foster, F. G. 1984. Ferns to Know and Grow. Timber Press, Inc., New York. pp. 172-174
25 Wetmore, R.H. 1953. Carbohydrate supply and leaf development in sporeling ferns. Science 118:578
26 Joung, J.A. 2005. In vitro masspropagation and mutation breeding of Pteridophyta native to Korea. Ph.D. Diss., Chungbuk Natl. Univ., Cheongju. (in Korean)