• 한국버섯학회지
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• 제13권3호
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• pp.170-174
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• 2015

식용버섯인 팽이버섯, 꽃송이버섯, 참송이버섯, 느타리 버섯, 녹각영지, 상황버섯, 눈꽃 동충하초, 아가리쿠스, 잎새버섯, 석이버섯, 노랑느타리버섯, 분홍느타리버섯, 편각영지버섯, 현미 동충하초, 번데기 동충하초, 표고버섯의 물 및 에탄올 추출물에 대한 피부염증완화 효능을 확인한 결과 열수추출물에서는 잎새버섯과 번데기 동충하초가 피부염증완화 효능이 탁월하였으며, 에탄올 추출물에서는 영지버섯, 표고버섯, 번데기 동충하초와 팽이버섯에서 피부염증완화 효능이 탁월하였다. 특히, 각각의 추출물에서 피부염증완화 효능이 높은 것은 번데기 동충하초 임을 확인하였다.

• Biomolecules & Therapeutics
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• 제25권4호
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• pp.427-433
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• 2017

Previously, we demonstrated that galangin (3,5,7-trihydroxyflavone) protects human keratinocytes against ultraviolet B (UVB)-induced oxidative damage. In this study, we investigated the effect of galangin on induction of antioxidant enzymes involved in synthesis of reduced glutathione (GSH), and investigated the associated upstream signaling cascades. By activating nuclear factor-erythroid 2-related factor (Nrf2), galangin treatment significantly increased expression of glutamate-cysteine ligase catalytic subunit (GCLC) and glutathione synthetase (GSS). This activation of Nrf2 depended on extracellular signal-regulated kinases (ERKs) and protein kinase B (AKT) signaling. Inhibition of GSH in galangin-treated cells attenuated the protective effect of galangin against the deleterious effects of UVB. Our results reveal that galangin protects human keratinocytes by activating ERK/AKT-Nrf2, leading to elevated expression of GSH-synthesizing enzymes.

• 한방안이비인후피부과학회지
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• 제26권1호
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• pp.50-62
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• 2013

Objective : Propionibacterium acnes (P. acnes) is a major pathogenic bacteria for acne vulgaris. This study was performed to evaluate the effects of Lithospermum erythrorhizon extracts on the inflammatory cytokines gene expression by P. acnes in human keratinocytes, HaCaT cell line. Methods : Anti-bacterial activity and cytotoxicity of LE extracts was analyzed by agar plate culture and XTT assay. The cytokines gene expressions were assessed by real time RT-PCR for IL-8, MCP-1 and TNF-${\alpha}$. During the cell culture and treatments, amounts of secreted TNF-${\alpha}$ were measured by ELISA. Translocation of transcription factor NF-${\kappa}B$ from cytoplasm into nucleus was observed by immunocytochemistry and confocal microscopy. Results : There were no anti-bacterial effects and cytotoxicity as high as $1,000{\mu}g/ml$ of LE extracts in XTT assay. Transcription levels of inflammatory cytokines, IL-8, MCP-1 and TNF-${\alpha}$ were increased by P. acnes in HaCaT. LE extracts decreased the upregulated gene transcription levels. However, amounts of secreted TNF-${\alpha}$ were similar in HaCaT cells with P. acnes and LE extracts. Translocation of NF-${\kappa}B$ into nucleus by P. acnes was significantly inhibited by LE extracts. Conclusions : From the results of this study, LE extracts have anti-inflammatory effects on HaCaT cells by P. acnes that decreased the mRNA expressions of IL-8, MCP-1 and TNF-${\alpha}$. This anti-inflammatory effects of LE extracts could provide the potential of therapeutic substance for acne vulgaris.

• 생명과학회지
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• 제33권5호
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• pp.414-421
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• 2023

갯사상자는 염생식물의 일종으로 염분이 높은 토양에서 자생하는 식물로 선행연구에 따르면 갯사상자는 항암효과를 가진다고 알려져 있다. 그러나 UVB로 유도된 광노화에 대한 효과는 아직 알려져 있지 않은 실정이다. 본 연구에서는 갯사상자 조추출물이 산화스트레스에 미치는 영향과 UVB 유래 광손상에 미치는 효과와 그 작용기전에 대하여 밝히고자 하였다. 갯사상자 추출물의 항산화 활성은 ROS 생성 억제능을 통해 확인하였으며, 광노화에 대한 효능은 피부노화 관련 인자인 MMP-1, MMP-3 및 MMP-9 발현 저해 효과와 MAPKs 발현기전을 통해 확인하였다. 세포생존율 실험결과를 바탕으로 UVB 조사 기준은 15 mJ/cm² 로 설정하였으며, 갯사상자 추출물의 농도는 10, 50, 그리고 100 ㎍/ml로 설정하였다. 결과적으로 갯사상자 추출물은 농도의존적으로 활성산소종을 억제하는 효과를 가짐을 확인하였고, 피부노화 관련인자인 MMP-1, MMP-3 및 MMP-9 발현을 감소시키는 것을 확인하였다. 노화관련인자인 MMP 발현 억제는 MAPK 전사인자의 발현 억제효과에 의한 것임을 확인할 수 있었다. 이들 결과로부터 갯사상자 추출물이 광노화에 효능을 가진 천연 화장품 소재로서 개발이 가능할 것으로 기대된다.

• Biomolecules & Therapeutics
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• 제24권3호
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• pp.312-319
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• 2016

Human skin cells undergo pathophysiological processes via generation of reactive oxygen species (ROS) upon excessive exposure to ultraviolet B (UVB) radiation. This study investigated the ability of hesperidin ($C_{28}H_{34}O_{15}$) to prevent apoptosis due to oxidative stress generated through UVB-induced ROS. Hesperidin significantly scavenged ROS generated by UVB radiation, attenuated the oxidation of cellular macromolecules, established mitochondrial membrane polarization, and prevented the release of cytochrome c into the cytosol. Hesperidin downregulated expression of caspase-9, caspase-3, and Bcl-2-associated X protein, and upregulated expression of B-cell lymphoma 2. Hesperidin absorbed wavelengths of light within the UVB range. In summary, hesperidin shielded human keratinocytes from UVB radiation-induced damage and apoptosis via its antioxidant and UVB absorption properties.

• 한국미생물·생명공학회지
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• 제49권4호
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• pp.528-533
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• 2021

The microalga, Tetrathelmis tetrathele, is used in the development of products for the aquaculture, food, and nutraceutical industries. In the present study, we investigated whether the T. tetrathele ethanolic extract (TTE), which has anti-inflammatory properties, can confer protection against alopecia and improve scalp health, influence the proliferation of human keratinocytes, HaCaT cells, and human hair follicle dermal papilla cells (HFDPC), or inhibit 5α-reductase activity. We found that TTE inhibited the production of the inflammatory mediator, nitric oxide (NO), and prostaglandin E₂ (PGE₂) without cytotoxicity in LPS-stimulated RAW 264.7 cells. In addition, TTE encouraged the proliferation of HaCaT cells and HFDPC. Our results showed that TTE had anti-inflammatory activities, proliferated HaCaT cells and HFDPC, and inhibited 5α-reductase activity. Therefore, we suggest that T. tetrathele could be a potent therapeutic agent for alopecia prevention and scalp improvement.

• 디지털융복합연구
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• 제14권11호
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• pp.639-644
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• 2016

본 연구의 목적은 발광 다이오드 (LED)을 인간 피부 각질 세포에 조사 시 콜라겐, 프로 콜라겐의 증식 발현을 조사하기 위해 실시되었었다. LED 조사 시 안전하게 인간의 피부에 적용할 수 있는지 여부를 결정하기 위해, LED 조사의 증식 효과는 인간 표피 각질세포에서 MTS 분석으로 결정하였다. 470nm의 파장 조사는 세포 독성 없이 mRNA의 콜라겐의 발현, 프로 콜라겐을 증가시켰으며, 이 결과는 470nm LED 조사가 피부각질세포 증식 효과와 콜라겐 합성에 영향을 미칠 수 있음을 시사한다. 또한 LED 조사시 독성 효과는 인간 피부 섬유 아세포 (HDF)에서 MTS 분석으로 결정 한 결과 세포 증식에 독성을 나타내지 않았다. 470nm LED 조사시 시험 관내 콜라겐 합성 활동을 증가시킴으로 피부미용 및 융복합적인 부분에 활용할 수 있는 기초 자료로 활용이 가능하다고 사료된다.

• BMB Reports
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• 제48권9호
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• pp.495-500
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• 2015

Up-regulation of cell adhesion molecules and proinflammatory cytokines contributes to enhanced monocyte adhesiveness and infiltration into the skin, during the pathogenesis of various inflammatory skin diseases, including atopic dermatitis. In this study, we examined the anti-inflammatory effects of butein, a tetrahydroxychalcone, and its action mechanisms using TNF-α-stimulated keratinocytes. Butein significantly inhibited TNF-α-induced ICAM-I expression and monocyte adhesion in human keratinocyte cell line HaCaT. Butein also decreased TNF-α-induced pro-inflammatory mediators, such as IL-6, IP-10 and MCP-1, in HaCaT cells. Butein decreased TNF-α-induced ROS generation in a dose-dependent manner in HaCaT cells. In addition, treatment of HaCaT cells with butein suppressed TNF-α-induced MAPK activation. Furthermore, butein suppressed TNF-α-induced NF-kappaB activation. Overall, our results indicate that butein has immunomodulatory activities by inhibiting expression of proinflammatory mediators in keratinocytes. Therefore, butein may be used as a therapeutic agent for the treatment of inflammatory skin diseases. [BMB Reports 2015; 48(9): 495-500]

• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.271-279
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• 2007

Black rice (Oryza sativa L. var. japonica) has been used in folk medicine in Asia. To understand the effects of black rice hydrolyzed peptides (BRP) from germinated black rice, we assessed the expression levels of about 20,000 transcripts in BRP-treated HaCaT keratinocytes using human 1A oligo microarray analysis. As a result, the BRP treatment showed a differential expression ratio of more than 2-fold: 745 were activated and 1,011 were repressed. One of the most interesting findings was a 2-fold increase in hyaluronan synthase 2 (HAS2) gene expression by BRP. Semiquantitative RT-PCR showed that BRP increased HAS2 mRNA in dose-dependent manners. ELISA showed that BRP effectively increased hyaluronan (HA) production in HaCaT keratinocytes.

• Journal of Plant Biotechnology
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• 제49권4호
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• pp.316-324
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• 2022

Hair loss causes psychological stress due to its effect on appearance. Therefore, the global market for hair loss treatment products is rapidly growing. The present study demonstrated that ginseng berry-derived and sequence-modified peptides promoted the proliferation rate of dermal papilla (DP) cells and keratinocytes, in addition to having antioxidant properties. Moreover, the potential role of these ginseng berry peptides as TGF-β2 antagonists was confirmed through in silico computer docking. In addition to promoting the growth of ,the ginseng berry-derived peptides also promoted the proliferation of keratinocytes experimental Particularly, an unmodified ginseng berry-derived peptide (GB-1) and two peptides with sequence modifications (GB-2 and GB-3) decreased ROS generation and exhibited a protective effect on damaged HaCaT keratinocytes. Computer-aided peptide discovery was conducted to identify the potential interactions of important proteins with transforming growth factor-beta 2 (TGF-β2), a key protein that plays a crucial role in the human hair growth cycle. Our results demonstrated that MAGH, an amino acid sequence present in herbal supplements and plant-based natural compounds, can inhibit TGF-β2.