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http://dx.doi.org/10.4062/biomolther.2015.139

Hesperidin Attenuates Ultraviolet B-Induced Apoptosis by Mitigating Oxidative Stress in Human Keratinocytes  

Hewage, Susara Ruwan Kumara Madduma (School of Medicine, Jeju National University)
Piao, Mei Jing (School of Medicine, Jeju National University)
Kang, Kyoung Ah (School of Medicine, Jeju National University)
Ryu, Yea Seong (School of Medicine, Jeju National University)
Han, Xia (School of Medicine, Jeju National University)
Oh, Min Chang (School of Medicine, Jeju National University)
Jung, Uhee (Radiation Biotechnology Research Division, Korea Atomic Energy Research Institute)
Kim, In Gyu (Department of Radiation Biology, Environmental Radiation Research Group, Korea Atomic Energy Research Institute)
Hyun, Jin Won (School of Medicine, Jeju National University)
Publication Information
Biomolecules & Therapeutics / v.24, no.3, 2016 , pp. 312-319 More about this Journal
Abstract
Human skin cells undergo pathophysiological processes via generation of reactive oxygen species (ROS) upon excessive exposure to ultraviolet B (UVB) radiation. This study investigated the ability of hesperidin ($C_{28}H_{34}O_{15}$) to prevent apoptosis due to oxidative stress generated through UVB-induced ROS. Hesperidin significantly scavenged ROS generated by UVB radiation, attenuated the oxidation of cellular macromolecules, established mitochondrial membrane polarization, and prevented the release of cytochrome c into the cytosol. Hesperidin downregulated expression of caspase-9, caspase-3, and Bcl-2-associated X protein, and upregulated expression of B-cell lymphoma 2. Hesperidin absorbed wavelengths of light within the UVB range. In summary, hesperidin shielded human keratinocytes from UVB radiation-induced damage and apoptosis via its antioxidant and UVB absorption properties.
Keywords
Apoptosis; Antioxidant; Hesperidin; Reactive oxygen species; Ultraviolet B;
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