[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5483/BMBRep.2015.48.9.259

Butein, a tetrahydroxychalcone, suppresses pro-inflammatory responses in HaCaT keratinocytes

Seo, Won Yong (Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University)
Youn, Gi Soo (Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University)
Choi, Soo Young (Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University)
Park, Jinseu (Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University)

Publication Information

BMB Reports / v.48, no.9, 2015 , pp. 495-500 More about this Journal

Abstract

Up-regulation of cell adhesion molecules and proinflammatory cytokines contributes to enhanced monocyte adhesiveness and infiltration into the skin, during the pathogenesis of various inflammatory skin diseases, including atopic dermatitis. In this study, we examined the anti-inflammatory effects of butein, a tetrahydroxychalcone, and its action mechanisms using TNF-α-stimulated keratinocytes. Butein significantly inhibited TNF-α-induced ICAM-I expression and monocyte adhesion in human keratinocyte cell line HaCaT. Butein also decreased TNF-α-induced pro-inflammatory mediators, such as IL-6, IP-10 and MCP-1, in HaCaT cells. Butein decreased TNF-α-induced ROS generation in a dose-dependent manner in HaCaT cells. In addition, treatment of HaCaT cells with butein suppressed TNF-α-induced MAPK activation. Furthermore, butein suppressed TNF-α-induced NF-kappaB activation. Overall, our results indicate that butein has immunomodulatory activities by inhibiting expression of proinflammatory mediators in keratinocytes. Therefore, butein may be used as a therapeutic agent for the treatment of inflammatory skin diseases. [BMB Reports 2015; 48(9): 495-500]

Keywords

Butein; ICAM-1; Inflammation; Keratinocyte; TNF-alpha;

Citations & Related Records

Times Cited By KSCI : 4 (Citation Analysis)

Reference
Cited By KSCI

1	Kwon DJ, Bae YS, Ju SM, Youn GS, Choi SY and Park J (2014) Salicortin suppresses lipopolysaccharide-stimulated inflammatory responses via blockade of NF-κB and JNK activation in RAW 264.7 macrophages. BMB Rep 47, 318-323 DOI
2	Ha SC, Han AR, Kim DW et al (2013) Neuroprotective effects of the antioxidant action of 2-cyclopropylimino-3-methyl-1,3-thiazoline hydrochloride against ischemic neuronal damage in the brain. BMB Rep 46, 370-375 DOI
3	Youn GS, Kwon DJ, Ju SM, Choi SY and Park J (2013) Curcumin ameliorates TNF-α-induced ICAM-1 expression and subsequent THP-1 adhesiveness via the induction of heme oxygenase-1 in the HaCaT cells. BMB Rep 46, 410-415 DOI
4	Shin SY, Kim CG and Lee YH (2013) Egr-1 regulates the transcription of the BRCA1 gene by etoposide. BMB Rep 46, 92-96 DOI
5	Lau GT, Huang H, Lin SM and Leung LK (2010) Butein downregulates phorbol 12-myristate 13-acetate-induced COX-2 transcriptional activity in cancerous and non-cancerous breast cells. Eur J Pharmacol 648, 24-30 DOI
6	Jang JH, Yang ES, Min KJ and Kwon TK (2012) Inhibitory effect of butein on tumor necrosis factor-α-induced expression of cell adhesion molecules in human lung epithelial cells via inhibition of reactive oxygen species gen- eration, NF-κB activation and Akt phosphorylation. Int J Mol Med 30, 1357-1364 DOI
7	Bito T, Roy S, Sen CK and Packer L (2000) Pine bark extract pycnogenol downregulates IFN-gamma-induced adhesion of T cells to human keratinocytes by inhibiting inducible ICAM-1 expression. Free Radic Biol Med 28, 219-227 DOI
8	Albanesi C and Pastore S (2010) Pathobiology of chronic inflammatory skin diseases: interplay between keratinocytes and immune cells as a target for anti-inflammatory drugs. Curr Drug Metab 11, 210-227 DOI
9	Nickoloff BJ, Griffiths CE and Barker JN (1990) The role of adhesion molecules, chemotactic factors, and cytokines in inflammatory and neoplastic skin disease--1990 update. J Invest Dermatol 94, 151S-157S DOI
10	Dhar A, Young MR and Colburn NH (2002) The role of AP-1, NF-kappaB and ROS/NOS in skin carcinogenesis: the JB6 model is predictive. Mol Cell Biochem 234-235, 185-193 DOI
11	Köhler HB, Huchzermeyer B, Martin M, De Bruin A, Meier B and Nolte I (2001) TNF-alpha dependent NF-kappa B activation in cultured canine keratinocytes is partly mediated by reactive oxygen species. Vet Dermatol 12, 129-137 DOI
12	Young CN, Koepke JI, Terlecky LJ, Borkin MS, Boyd Savoy L and Terlecky SR (2008) Reactive oxygen species in tumor necrosis factor-alpha-activated primary human keratinocytes: implications for psoriasis and inflammatory skin disease. J Invest Dermatol 128, 2606-2614 DOI
13	Pastore S, Mascia F, Mariotti F, Dattilo C, Mariani V and Girolomoni G (2005) ERK1/2 regulates epidermal chemokine expression and skin inflammation. J Immunol 174, 5047-5056 DOI
14	Kaminska B (2005) MAPK signalling pathways as molecular targets for anti-inflammatory therapy--from molecular mechanisms to therapeutic benefits. Biochim Biophys Acta 1754, 253-262 DOI
15	Kwon DJ, Bae YS, Ju SM, Goh AR, Choi SY and Park J (2011) Casuarinin suppresses TNF-α-induced ICAM-1 expression via blockade of NF-κB activation in HaCaT cells. Biochem Biophys Res Commun 409:780-785 DOI
16	Gloire G, Legrand-Poels S and Piette J (2006) NF-κB activation by reactive oxygen species: fifteen years later. Biochem Pharmacol 72, 1493-1505 DOI
17	Yadav VR, Prasad S, Sung B and Aggarwal BB (2011) The role of chalcones in suppression of NF-κB-mediated inflammation and cancer. Int Immunopharmacol 11, 295-309 DOI
18	Cheng ZJ, Kuo SC, Chan SC, Ko FN and Teng CM (1998) Antioxidant properties of butein isolated from Dalbergia odorifera. Biochim Biophys Acta 1392, 291-299 DOI
19	Lee SH, Seo GS and Sohn DH (2004) Inhibition of lipopolysaccharide-induced expression of inducible nitric oxide synthase by butein in RAW 264.7 cells. Biochem Biophys Res Commun 323, 125-132 DOI
20	Pandey MK, Sandur SK, Sung B, Sethi G, Kunnumakkara AB and Aggarwal BB (2007) Butein, a tetrahydroxychalcone, inhibits nuclear factor (NF)-kappaB and NF-kappaBregulated gene expression through direct inhibition of IkappaBalpha kinase beta on cysteine 179 residue. J Biol Chem 282, 17340-17350 DOI
21	Gröne A (2002) Keratinocytes and cytokines. Vet Immunol Immunopathol 88, 1-12 DOI
22	Dustin ML, Singer KH, Tuck DT and Springer TA (1988) Adhesion of T lymphoblasts to epidermal keratinocytes is regulated by interferon gamma and is mediated by intercellular adhesion molecule 1 (ICAM-1). J Exp Med 167, 1323-1340 DOI
23	Sebastiani S, Albanesi C, De PO, Puddu P, Cavani A and Girolomoni G (2002) The role of chemokines in allergic contact dermatitis. Arch Dermatol Res 293, 552-559 DOI
24	Song HY, Lee JA, Ju SM et al (2008) Topical transduction of superoxide dismutase mediated by HIV-1 Tat protein transduction domain ameliorates 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice. Biochem Pharmacol 75, 1348-1357 DOI

	Jiae Kim. (2017) Brain Research Bulletin Neuroprotective effects of N-adamantyl-4-methylthiazol-2-amine against amyloid β-induced oxidative stress in mouse hippocampus / 128 , 22
	Ganesan Padmavathi. (2017) Phytomedicine Butein in health and disease: A comprehensive review / 25 , 118
13	Ganesan Padmavathi. (2015) Phytomedicine Potential of butein, a tetrahydroxychalcone to obliterate cancer / 22 (13) , 1163
11	Hyo Sang Jo. (2016) Medicinal Chemistry Research Tat-DJ-1 inhibits oxidative stress-mediated RINm5F cell death through suppression of NF-κB and MAPK activation / 25 (11) , 2589
3	Young-Su Yi. (2017) The Korean Journal of Physiology & Pharmacology JS-III-49, a hydroquinone derivative, exerts anti-inflammatory activity by targeting Akt and p38 / 21 (3) , 345
12	Yoon-Young Sung. (2016) PLOS ONE Forsythia suspensa Suppresses House Dust Mite Extract-Induced Atopic Dermatitis in NC/Nga Mice / 11 (12) , e0167687
4	Hyo Sang Jo. (2017) Biotechnology Letters Tat-DJ-1 enhances cell survival by inhibition of oxidative stress, NF-κB and MAPK activation in HepG2 cells / 39 (4) , 511
11	Hyo Sang Jo. (2016) BMB Reports Protective effects of Tat-NQO1 against oxidative stress-induced HT-22 cell damage, and ischemic injury in animals / 49 (11) , 617
	Min Jea Shin. (2016) Free Radical Biology and Medicine Tat-PRAS40 prevent hippocampal HT-22 cell death and oxidative stress induced animal brain ischemic insults / 97 , 250
5	Hyo Sang Jo. (2016) BMB Reports Transduced Tat-DJ-1 protein inhibits cytokines-induced pancreatic RINm5F cell death / 49 (5) , 297
	Wenhao Zheng. (2017) International Immunopharmacology Butein inhibits IL-1β-induced inflammatory response in human osteoarthritis chondrocytes and slows the progression of osteoarthritis in mice / 42 , 1
11	(2017) BMB Reports Protein tyrosine phosphatase PTPN21 acts as a negative regulator of ICAM-1 by dephosphorylating IKKβ in TNF-α-stimulated human keratinocytes / 50 (11) , 584
4	(2018) Pharmaceuticals Effects of Boswellia Serrata Roxb. and Curcuma longa L. in an In Vitro Intestinal Inflammation Model Using Immune Cells and Caco-2 / 11 (4) , 126