• Microbiology and Biotechnology Letters
• /
• v.22 no.1
• /
• pp.37-44
• /
• 1994

The homology among the genes coding for degradation of bipheny(BP) and 4-chlorobiphenyl(4CB) was comparatively analyzed by Southern hybridization in several BP/4CB degrading bacterial strains. As the hybridization results of their genomic DNAs with pcbABCD as the DNA probe, the group of Pseudomonas sp. DJ-12. P08 and P27 strain was separated by the group of P20 and P1242 strains. The P. pseudoalcaligenes KF707 showed the hybidization signal which was homologous to the group of DJ-12, but they had different restriction endonuclease sites. The pcbAB genes in pCUl recombinant plasmid from Pseudomonas sp. DJ-12 appeared to be homologous to pchAB genes in pKTF20 cloned from P. pseudoalcaligenes KF707, but the C genes in both strains were not homologous. The bphABC in pKTF20 showed the signals homologous to the cbp ACB in pAW6194 cloned from P. putida OU83, but homologous signal was not found botween the pcbABCD genes in pCUl and the cbpADCB genes in pAW6194 recombbinant plasmid.

• Bulletin of the Korean Mathematical Society
• /
• v.48 no.1
• /
• pp.129-140
• /
• 2011

The aim of this paper is to study the structure of the fundamental group of a closed oriented Riemannian manifold with positive scalar curvature. To be more precise, let M be a closed oriented Riemannian manifold of dimension n (4 $\leq$ n $\leq$ 7) with positive scalar curvature and non-trivial first Betti number, and let be $\alpha$ non-trivial codimension one homology class in $H_{n-1}$(M;$\mathbb{R}$). Then it is known as in [8] that there exists a closed embedded hypersurface $N_{\alpha}$ of M representing $\alpha$ of minimum volume, compared with all other closed hypersurfaces in the homology class. Our main result is to show that the fundamental group ${\pi}_1(N_{\alpha})$ is always virtually free. In particular, this gives rise to a new obstruction to the existence of a metric of positive scalar curvature.

• East Asian mathematical journal
• /
• v.10 no.1
• /
• pp.109-122
• /
• 1994

• Journal of the Korean Mathematical Society
• /
• v.35 no.2
• /
• pp.281-294
• /
• 1998

In this paper, we extend the G-sequence of a CW-pair to the G-sequence of a map and show the existence of a map with nonexact G-sequence. We also give an example of a finite CW-pair with nontrivial $\omega$-homology in high order.

• Korean Journal of Veterinary Research
• /
• v.43 no.2
• /
• pp.219-230
• /
• 2003

Porcine epidemic diarrhea virus(PED), a member of Coronaviridea, is the etiological agent of enteropathogenic diarrhea in swine. The purpose of this study was to investigate genetic characteristic of PEDV isolated in Korea. Nucleocapsid(N) gene and membrane (M) gene of recent Korean PEDV strains isolated in 2001 were amplified, cloned, sequenced and analyzed. N gene of seven Korean PEDV field isolates bad 94.5% to 99.4% nucleotide and 92.4% to 99.4% amino acid sequence homology each other. Nucleotide and amino acid sequences of Korean field PEDVs were different from published foreign PEDVs, showing 95.1% to 98.0% nucleotide and 93.5% to 97.6% amino acid sequence homology. By phylogenetic tree analysis on based nucleotide sequences, PEDVs were clustered into four groups. By phylogenetic tree analysis based on amino acid sequences. PEDVs were clustered into five groups. M gene of our Korean PEDV field isolates had 99.6% to 100% nucleotide and 98.7% to 100% amino acid sequence homology each other. Nuclotide and amino acid sequences of Korean field PEDVs were different from published foreign PEDVs, showing 98.5% to 98.8% nucleotide and 97.3% to 97.8% amino acid sequence homology. By phylogenetic tree analysis based on nucleotide and amino acid sequences, PEDVs were clustered into two groups which were Korean PEDV isolate group and foreign PEDV isolate group.

• Bulletin of the Korean Mathematical Society
• /
• v.33 no.2
• /
• pp.253-258
• /
• 1996

Let X be a finite connected CW-complex, $\Gamma = \pi_1(X)$ its fundamental group, $\tilde{X}$ its universal covering space. Then $\Gamma$ acts on $\tilde{X}$ by covering transformations and on the homology group $H_*(\tilde{X})$. In this note we establish the following vanishing result for the Euler characteristic $x(X)$ of X.

• Proceedings of the Korean Society for Applied Microbiology Conference
• /
• 2001.06a
• /
• pp.163-164
• /
• 2001

A number of bacterial species produce extracellular lipases. Among them, many lipase genes have been cloned and sequenced. A comparison of primary sequences revealed only very limited sequence homology among them. Based on the sequence homologies and molecular sizes (Mr), bacterial lipases were classified into four discrete groups. From soil samples taken around Taejon, five different lipase-producing bacteria were isolated; Proteus vulgaris K80, Bacillus stearothermophilus Ll, B. pumilus B26, Staphylococcus haemolyticus L62, S. aureus B56. Nucleotide sequence analysis showed that Staphylococcus lipase genes (L62 and B56) composed of pre-pro-mature parts, Bacillus lipase genes (Ll and B26) pre-mature parts, and Proteus lipase gene (K80) mature part only. In addition, the molecular sizes of their mature parts were quite different from 19,000 to 45,000. Finally, they had very little homology (less than 20％) in their amino acid sequences. Judging from the above results, lipase K80 belonged to bacterial lipase Group I, lipase L1 and lipase B26 Group III, and lipase L62 and lipase B56 Group IV. This diversity in their primary structures was also reflected in their enzymatic properties; temperature effects, pH effects, substrate specificity, detergent effects, and so on.

• Korean Journal of Mathematics
• /
• v.19 no.4
• /
• pp.409-421
• /
• 2011

We study a natural map from the braid group to the mapping class group which is called Harer map. It is rather new and different from the classical map which was studied in 1980's by F. Cohen, J. Harer et al. We show that this map is homologically trivial for most coefficients by using the fact that this map factors through the symmetric group.

• Journal of the Korean Mathematical Society
• /
• v.54 no.5
• /
• pp.1411-1440
• /
• 2017

We study free actions of finite groups on 3-dimensional nil-manifolds with the first homology ${\mathbb{Z}}^2{\oplus}{\mathbb{Z}}_p$. By the works of Bieberbach and Waldhausen, this classification problem is reduced to classifying all normal nilpotent subgroups of almost Bieberbach groups of finite index, up to affine conjugacy.

• The Korean Journal of Mycology
• /
• v.25 no.1 s.80
• /
• pp.46-67
• /
• 1997

From 1985 to 1993, we collected 20 isolates throughout Kangwon and obtained 6 isolates from other sources. A. mellea formed rhizomorph actively, and some of A. osroyae were poor in the formation of rhizomorph and some without formation of rhizomorph. A. tabescens was active in the growth of aerial mycelium and poor in the development of rhizomorph. In A. gallica, the mycelium development among the isolates were variable greatly, and especially in isolate A8(KNU-250), the mycelial development was similar to that of A. osroyae, but A8(KNU-250) showed the feature of A. gallica to change medium into brown color. In PCR-RFLP analysis of the IGS region in rDNA, the homology between each isolate in the A. mellea and A. ostoyae showed 100% homology. A. tabescens showed $0.919{\sim}0.974$ homology, and A. gallica showed $0.619{\sim}1.000$ homology. A19 and A12 showed 100% homology as the same group, but compared with other subgroups they showed less than 10% homology as $0.051{\sim}0.108$ value. In RAPD analyses, the isolates of A. mellea showed high homology among themselves as $0.983{\sim}1.000$, and A. ostoyae also showed high similarity. The homology between isolates of A. tabescens showed $0.594{\sim}0.953$ value because A. gallica showed $0.280{\sim}0.733$ value, and the variations between isolates were greater than other species. Especially, A19 and A22 were identified as new novel group which were remoted from other groups, and the homology between these two isolates showed 0.921 value, and the genetic similarity between these groups and other 4 groups showed less than 7% as $0.012{\sim}0.069$ value. Of 5 species identified in this study, 4 species were identified as A. mellea, A. ostoyae, A. tabescens, and A. gallica that were already reported ones and 1 species was suggested as a new specie in Korea.