• Journal of Microbiology and Biotechnology
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• v.32 no.4
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• pp.464-472
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• 2022

An endo-polygalacturonase (endo-PGase) exhibiting excellent performance during acidic fruit juice production would be highly attractive to the fruit juice industry. However, candidate endo-PGases for this purpose have rarely been reported. In this study, we expressed a gene from Penicillium oxalicum in Pichia pastoris. The recombinant enzyme PoxaEnPG28C had an optimal enzyme activity at pH 4.5 and 45℃ and was stable at pH 3.0-6.5 and < 45℃. The enzyme had a specific activity of 4,377.65 ± 55.37 U/mg towards polygalacturonic acid, and the K_m and V_max values of PoxaEnPG28C were calculated as 1.64 g/l and 6127.45 U/mg, respectively. PoxaEnPG28C increased the light transmittance of orange, lemon, strawberry and hawthorn juice by 13.9 ± 0.3%, 29.4 ± 3.8%, 95.7 ± 10.2% and 79.8 ± 1.7%, respectively; it reduced the viscosity of the same juices by 25.7 ± 1.6%, 52.0 ± 4.5%, 48.2 ± 0.7% and 80.5 ± 2.3%, respectively, and it increased the yield of the juices by 24.5 ± 0.7%, 12.7 ± 2.2%, 48.5 ± 4.2% and 104.5 ± 6.4%, respectively. Thus, PoxaEnPG28C could be considered an excellent candidate enzyme for acidic fruit juice production. Remarkably, fruit juice production using hawthorn as an material was reported for the first time.

• Korean journal of applied entomology
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• v.51 no.2
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• pp.131-140
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• 2012

The hawthorn spider mite, Tetranychus viennensis, is a pest of apples and a quarantine pest from some countries that import apples from Korea. A controlled atmosphere and temperature treatment system (CATTS) was developed as an alternative disinfestation method to methyl bromide fumigation treatment, and has been applied to control various insects and other arthropod pests on fruits. We applied CATTS to disinfect T. viennensis under conditions that were previously developed to control the peach fruit moth, Carposina sasakii. First, T. viennensis was sampled from Japanese apricot, Prunus mume, and identified by its morphological characters. In addition, both cytochrome oxidase I (COI) and internal transcribed spacer (ITS) sequences supported the morphological identification. Second, the heat-tolerant developmental stage was determined in T. viennensis. When a $46^{\circ}C$ heat treatment was applied to egg, nymph, and adult stages of T. viennensis, adults were the most tolerant stage. Third, when heat temperature was used along with 1% $O_2$ and 15% $CO_2$, the mites showed a significant increase in susceptibility to the heat treatment. Finally, CATTS at $46^{\circ}C$ with 15% $CO_2$ and 1% $O_2$ for 30 min resulted in 100% mortality of all T. viennensis development stages. These results indicated that CATTS isapplicable to disinfest T. viennensis in post-harvest apples.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.53.2-53.2
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• 2015

Fiber Bragg gratings (FBGs) are the most compact and reliable method of suppressing atmospheric emission lines in the infrared for ground-based telescopes. It has been proved that real FBGs based filters were able to eliminate 63 bright sky lines with minimal interline losses in 2011 (GNOSIS). Inscribing FBGs on multi-core fibers offers advantages. Compared to arrays of individual SMFs, the multi-core fiber Bragg grating (MCFBG) is greatly reduced in size, resistant to damage, simple to fabricate, and easy to taper into a photonics lantern (PRAXIS). Multi-mode fibers should be used and the number of modes has to be large enough to capture a sufficient amount of light from the telescope. However, the fiber Bragg gratings can only be inscribed in the single-mode fiber. A photonic lantern bi-directionally converts multi-mode to single-mode. The number of cores in MCFBGs corresponds to the mode. For a writing system consisting of a single ultra-violet (UV) laser and phase mask, the standard writing method is insufficient to produce uniform MCFBGs due to the spatial variations of the field at each core within the fiber. Most significant technical challenges are consequences of the side-on illumination of the fiber. Firstly, the fiber cladding acts as a cylindrical lens, narrowing the incident beam as it passes through the air-cladding interface. Consequently, cores receive reduced or zero illumination, while the focusing induces variations in the power at those that are exposed. The second effect is the shadowing of the furthest cores by the cores nearest to the light source. Due to a higher refractive index of cores than the cladding, diffraction occurs at each core-cladding interface as well as cores absorb the light. As a result, any core that is located directly behind another in the beam path is underexposed or exposed to a distorted interference pattern from what phase mask originally generates. Technologies are discussed to overcome the problems and recent experimental results are presented as well as simulation results.

• Transactions of the Korean Society of Mechanical Engineers
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• v.15 no.1
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• pp.229-240
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• 1991

The large camber angle theory of turbomachine blade of compressor has been developed recently for the two-dimensional flow by Hawthorn, et al. However, in the above theory it was assumed that the fluid was incompressible and inviscid, and the blades had no thickness. In this study, the flow in a blade cascade being mounted in parallel fashion with blade of arbitrary thickness is studied in order to determine the effects of the camber angle on the performance characteristic of the blade section under the consideration of compressibility and viscosity of fluid. The panel method is used for potential flow analysis. The flow in the boundary-layer is obtained by solving the integral boundary-layer structure through the laminar, transitional , and turbulent flow using the pressure field determined from the potential flow. And then the viscous-inviscid interaction scheme is used for interaction of these two flows. For the determination of the variation in the outlet fluid angle influenced by deviation in cascade flow, the superposition method which is used for single foil is introduced in this analysis. By the introduction of this method, the effects of the deviation on outlet fluid angle and the resulting fluid angle are made to adjust for oneself through the calculation. As the result of this study, the blade of large camber angle, large incidence angle, large pitch-chord ratio has large viscous and compressible effect than those of small camber angle. Lift force increase as camber angle increases, but above 60.deg. of camber angle, lift force decrease as camber angle increases. But drag force increases linearly with camber angle increases in the entire region.

• Korean Journal of Food Science and Technology
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• v.38 no.6
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• pp.779-784
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• 2006

To produce bio-active Kochujang, 1.2% of pomegranate powder, 1.0% of hawthorn berry extract powder, 0.01% of ginko biloba extract, 1.2% of red yeast rice powder, 1.2% of silk powder, 0.2% of garlic oil, 0.2% of chitosan, 1.2% of wax gourd extract powder, and 1.0% of pine mushroom were added individually with raw materials that are needed for Kochujang process. These Kochujangs were fermented for 45 days at $30^{\circ}C$, and examined for changes in their physicochemical properties. There were no significant differences in pH, acidity, formol-N content, color and total cell count by plate count agar between Kochujangs fortified individually with functional ingredients and the control Kochujang. When 0.2% of garlic oil was added, antioxidant activity of its Kochujang product increased twice as much as that of the control Kochujang. Improved ACE inhibiting activities were observed in 1.2% of red yeast rice powder. When 1.2% of silk powder or 0.01% of ginko biloba extract were added, antithrombotic abilities were remarkably enhanced up to two times of the control Kochujang.

• The Korea Journal of Herbology
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• v.32 no.1
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• pp.55-62
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• 2017

Objectives : Crataegi Fructus has been used as traditional medicines for more digestion action, amenorrhea due to blood stasis, and hyperlipemia. The aim of this study was to compare of Crataegi Fructus in South Korea collected during three years according to the standards in monographs of the Korean Pharmacopoeia Eleventh edition (KP 11). Methods : Crataegi Fructus was carried out identification test (Qualitative reaction, Thin layer chromatography), heavy metal test, and total ash registered at KP. Add to we tested loss on dry, contents of ethanol-soluble extracts, and HPLC profiling. Results : Identification test (TLC) was on comparing with ursolic acid standard solution in $R_f$ value, all samples showed red purple spot ($R_f$ value 0.9). Ursolic acid spot in $R_f$ value 0.35 showed by changing mobile phase condition. Heavy metals showed contents for Pb, Cd, As, and Hg range of 0.0 ~ 0.5 ppm, 0.0 ~ 0.2 ppm, 0.0 ~ 0.3 ppm, and 0.0 ~ 0.1 ppm. Loss on drying was ranged from 5.5 to 11.9 %, total ash was between the range 2.7 ~ 4.0 %. Contents of ethanol-soluble extracts was ranged from 17.8 to 44.9 %. The content of chlorogenic acid was ranged from 0.0 to 0.1 % based on the chlorogenic acid standard curve. Conclusion : We have verified the current specification standard of Crataegi Fructus and standard that is not set. We hope that it will help the standardization of Crataegi Fructus.

• Korean journal of food and cookery science
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• v.22 no.4 s.94
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• pp.428-437
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• 2006

To investigate the worth of herbs as functional food ingredients, the antioxidant activity of 15 kinds of herb mathanol extracts was evaluated. Green tea, chamomile, dandelion, and lemon vervena extracts, with IC$_{50}$ values of 1.45 g/100mL, 1.49 g/100mL, 1.50 g/100mL and 1.55 g/100mL, respectively, had significantly higher superoxide radical scavenging activity than any other herb extracts. Green tea and lemon vervena extracts, which had high radical scavenging activity, showed inhibition of cell proliferation in Chinese hamster lung fibroblasts (V79-4 cells). Most herb extracts, except for chamomile, fennel and dandelion enhanced cell viability against H$_2$O$_2$-induced oxidative damage in V79-4 cells. At a dose of 1600 ${\mu}$g/mL, lemon vervena, green tea, hawthorn and rosemary extracts showed a cell viability of more than 50% which was significantly higher than that of the control culture treated with only H$_2$O$_2$ Thus, the results suggest that some herb extracts exhibited a V79-4 cell protective effect. The investigation of the cellular antioxidant enzymes activities of the five selected herb extracts revealed that extracts of lemon vervena and chamomile dose-dependently increased superoxide dismutase and glutathione peroxidase activity but that this increase was not significant. In conclusion, some natural herb extracts exhibited high antioxidant activity.

• Korean Journal of Medicinal Crop Science
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• v.11 no.2
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• pp.135-142
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• 2003

This study was conducted to clarify on germination rate enhancement effect and to develope the growing seedling method on Sorbus commixta Hedl. The results were follows; The optimum temperature of stratification and germination of mountain ash was $5^{\circ}C$ the seed germination rate in wet stratification with sand at $5^{\circ}C$ for 90 days was 22.6% and the highest germination of 78.9% was observed when seed was soaked in BA 200ppm for 20 minute after stratification for 90 days at $5^{\circ}C$. The highest germination of large chinese hawthorn was 11.1% when seed was soaked BA 50ppm for 20 minute after stratification 90 days at $5^{\circ}C$. Emergence ratio in box raising seedling and pot seedling showed high, 93 and 95%. Root length and dry root weight in pot seedling for 50 days showed higher than that of direct seedling and box raising seedling, Plant height and dry leaf weight in box raising seedling showed higher than that of pot seedling and direct seedling. The growth increment of seedling was high between 40 days and 50 days after seedling. The excellent seedling of mountain ash was produced in pot seedling for 50 days.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.39 no.3
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• pp.1-14
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• 2021

This study attempted to examine the loving pigeon culture practiced in traditional gardens and to illuminate the aspect of pigeons as a landscape animal material. In order to understand the culture of enjoying old garden through pigeons, the contents were analyzed for the translated version of the old literatures and paintings. Pigeons have been used as Jeonseo-gu(傳書鳩) and also for medicinal purposes and food. Pigeons have various symbolic meanings such as abundance, hospitality, and longevity. From the Goryeo Dynasty to the early Joseon Dynasty, pigeons were raised in the palace and private garden. In the late Joseon Dynasty, temporary trend of ornamental pigeon culture occurred. Pigeons were synesthesia materials that enriched the forest. Various beautiful pigeons created a variable landscape of the primeval forest as a moving landscape material. The bell sounds that appear differently depending on the pigeon's movement led to a rich auditory experience of the landscape. The pigeon house was an ornamental element that enriched the old garden along with the pigeon. The owners of garden were involved in gardening through the act of buying a pigeon house and placing it in the garden or making a pigeon house themselves. In addition, the writers planted plum trees, peach trees, apricot trees, and hawthorn trees as a symbol of spring and a source of food for pigeons, and expressed them in poems and paintings. This study has a limitation in that the translation of the old text was used as an analysis data. The follow-up studies on specific cases of raising pigeons in the old garden, in modern and contemporary landscape spaces are urged.

• Research in Plant Disease
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• v.30 no.2
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• pp.139-147
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• 2024

Erwinia amylovora is a gram-negative plant pathogen that causes fire blight in apple and pear trees, resulting in significant damage worldwide. In this study, we monitored the emergence of fire blight from 2019 to 2023 to determine the emergence patterns and the factors affecting the outbreak of the disease. As a result of the 5-year survey on the emergence of fire blight, a total of 2,029 cases have emerged, mostly in apple trees of 1,378 cases (67.9%) followed by 645 cases (31.8%) in pear trees, and from quince, hawthorn, and mountain ash trees. Fire blight appeared in specific areas of Gyeonggi, Chungnam, Gangwon, and Chungbuk provinces in 2019, but spread to Andong and Yesan in 2021, Muju and Bonghwa in 2023. In 2020 and 2021, there were 744 and 618 cases of fire blight outbreaks, respectively, compared to other years (188-245 cases/year). Notably, 914 of these cases were observed in apple trees from May to July, with 667 cases reported in Chungju and Jecheon. The incidence of fire blight was positively correlated with the daily maximum temperatures and rainy days in January and February, as well as the rainy days in May and June. The average age of the diseased pear trees was 25 years, higher than the 10-year average age of the apple trees. This study provides fundamental information to understand the status and factors affecting the fire blight emergence in Korea. Prevention measures should be established through continuous analysis of the status of fire blight.