• Animal Bioscience
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• 제34권6호
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• pp.1078-1087
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• 2021

Objective: Skeletal muscle satellite cells (SMSCs) are significant for the growth, regeneration, and maintenance of skeletal muscle after birth. However, currently, few studies have been performed on the isolation, culture and inducing differentiation of goose muscle satellite cells. Previous studies have shown that C1q and tumor necrosis factor-related protein 3 (CTRP3) participated in the process of muscle growth and development, but its role in the goose skeletal muscle development is not yet clear. This study aimed to isolate, culture, and identify the goose SMSCs in vitro. Additionally, to explore the function of CTRP3 in goose SMSCs. Methods: Goose SMSCs were isolated using 0.25% trypsin from leg muscle (LM) of 15 to 20 day fertilized goose eggs. Cell differentiation was induced by transferring the cells to differentiation medium with 2% horse serum and 1% penicillin streptomycin. Immunofluorescence staining of Desmin and Pax7 was used to identify goose SMSCs. Quantitative realtime polymerase chain reaction and western blot were applied to explore developmental expression profile of CTRP3 in LM and the regulation of CTRP3 on myosin heavy chains (MyHC), myogenin (MyoG) expression and Notch signaling pathway related genes expression. Results: The goose SMSCs were successfully isolated and cultured. The expression of Pax7 and Desmin were observed in the isolated cells. The expression of CTRP3 decreased significantly during leg muscle development. Overexpression of CTRP3 could enhance the expression of two myogenic differentiation marker genes, MyHC and MyoG. But knockdown of CTRP3 suppressed their expression. Furthermore, CTRP3 could repress the mRNA level of Notch signaling pathway-related genes, notch receptor 1, notch receptor 2 and hairy/enhancer-of-split related with YRPW motif 1, which previously showed a negative regulation in myoblast differentiation. Conclusion: These findings provide a useful cell model for the future research on goose muscle development and suggest that CTRP3 may play an essential role in skeletal muscle growth of goose.

• 유통과학연구
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• 제17권10호
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• pp.107-114
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• 2019

Purpose - This is longitudinal research which aims to investigate the meaning of greenness to consumers' behavior. Consumers adopt green marketing as a new factor in product buying and consumption and more and more consumers prefer green product and services. Consumers' green buying behavior can be different from other purchasing experiences. There would be changes in the meaning of green as time passed and it can be different from countries to other countries. This study examines focus group studies with several groups. There is a ten-year gap between 2010 focus group and 2019 focus group interviews. With this ten-year gap, we can find the change of greenness to consumers. Research design, data, and methodology - The data were collected from Turkish, Korean, Kazakhstan people. This is a cross-sectional study and focus group interview was designed. We can gain information relevant to the research problem with using focus group study and get some insights into basic needs and attitudes of green marketing. The subjects for green purchase interviewee were confined to under 40 years old's shoppers regardless of gender. The first study was investigated with several groups in 2010 and the second interview were conducted in 2019. Results - Results show that the meaning of greenness for consumer has changed over time in accordance with the growing accordance of environmental sustainability. Basically, green marketing still means valuable, natural, recycle-able, good for health, clean, smart behavior, essential benefit. The concept of greenness significantly evolved since it was investigated in 2010. It moves away from focusing on specific environmental issues to considering global sustainability issues. Especially we found that greenness can be related with globalization, higher education, social status at the 2019 interview. Conclusions - This paper attempted to confirm the green marketing is essential and expands its meaning to various aspect. Usually, we can think green marketing is everywhere, therefore, people don't care about green issues in real. But consumers are adopting green marketing more and more, it can be a means to attract potential consumers. Therefore, companies should provide enough greenness information for people and they might apply greenness communication to attract potential customers.

• 한국환경복원기술학회지
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• 제4권3호
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• pp.75-83
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• 2001

This study selected Yangpyung as a target site because Yangpyung is a area of high value blessed with well preserved natural resources and beautiful natural scene and where thoughtless development and malformed use of land are in progress under the mask of hotel, accommodations and sales facility which fits the interests of land owner. As for the method used for this study, I inquired into domestic/international instances and the concept of existing environmental indicators and assessment of suitability with a view to establish assessment indicators and pose the concept through theoretical investigation of green-network and to establish green-network. 17 articles of environmental indicators such as aspect analysis, contour, greenbelt, DEM, NDVI, nature conservation area, reservoir and area for the promotion of agriculture were chosen as a actual analysing n data for setting up assessment indicators. From the result of analysis, as anticipated, green zone in Yongmusan areas within Youngmun and Seojong areas were the center of green-network in the wide area green-network in Yangpyung and the restricted area by basin system, road and legal regulation was selected as spot area and finally arable land and reservoir were selected as base which connects core with spot.

• 한국실내디자인학회논문집
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• 제20권6호
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• pp.11-18
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• 2011

Including interior architecture, diverse fields of study for eco-friendly environment is in progress due to the world's crisis consciousness of global environment. And nevertheless, practical use of the research results are still low and systematical problems of the studies are inevitable reality. Thus, this study focused on to the enbodiment of green interior architecture study understanding the problems of precedence studies to make suggestion for the future direction and balancing green interior architecture study seeking for better motivation. Analysing results of the precedent studies indicates that the existing studies loss its diversity and concentrated in the narrow based directions. For the enbodiment of green interior architecture, not simply immerse existing planning method found from case study, the study need to perform in overall areas of green interior architecture and diversify its direction. Besides space designer, user's consciousness are also important results to form a new paradigm of eco-friendly environment. For the applications and use of the study results as the design factor in green interior architecture, first and the most important stept is to innovate attitude towards the nature and shift of consciousness through education as well as the relative studies of design and planning realm to drive both technical, and non-technical design factors are highly demanded.

• 정보관리학회지
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• 제32권3호
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• pp.99-130
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• 2015

본 연구에서는 친환경 녹색도서관을 인증함에 있어 도서관의 친환경성을 평가할 수 있는 평가지표를 개발하여 제안하고자 하였다. 이를 위해 녹색도서관 관련 문헌 및 사례조사를 통해 도서관 녹색요소를 추출하였으며, 평가요소에 대한 도서관 직원들의 인식을 설문조사하였다. 최종적으로 11개의 평가영역, 31개의 평가항목, 135개의 평가지표를 가진 녹색도서관 평가지표를 개발하였다. 11개의 평가영역은 토지이용 및 교통, 에너지 및 환경오염 방지, 재료 및 자원, 물순환 관리, 유지관리, 생태환경, 실내환경, 도서관 자원, 친환경 교육프로그램 및 캠페인, 직원 및 운영, 전산화 등이다.

• KIEAE Journal
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• 제12권1호
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• pp.45-53
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• 2012

Our society confronts a new social demand to protect the environmental equity of the people, and the green open space that is a representative public property which takes a very important role for the residents in less privileged urbane area in terms of providing amenity without requiring individual expenses additionally. This study aims to identify the current status and problems based on the environmental experiences of the actual residents, excluding the simple physical status, in relation with supplying public property of green open space, and the study put focus on the old downtown area of Jeonju-si which is a test-bed of urbane regeneration planned by the government. Residents in less privileged area evaluate the importance of green environment of park very highly as a factor to enhance their quality of life, but as they do not have well-established environmental infrastructure to back up their needs practically, they face difficulties to use such environment and show low level of satisfaction. In particular, as such characteristics appear remarkably among the least privileged urbane people in the less privileged urbane area, their dependence and expectation upon the environment of green open space is very apparent and, therefore, it is urgently needed to improve their environment for public green park area for the less privileged urbane people.

• The Journal of Asian Finance, Economics and Business
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• 제8권9호
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• pp.79-89
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• 2021

Green finance plays an important role in environmental protection missions and fighting climate change. The Environment Fund in Vietnam is the main channel of preferential capital offered to firms for environmental protection. Unfortunately, it was previously unknown which criteria influenced these companies' ability to obtain green financing. Using a survey method, we collected data through a structured questionnaire of 203 respondents that represent firms that had received concessional loans from 26 Environment Funds. A Multiple Linear Regression model was used to examine the determinants of access to concessional loans for environmental protection. We found relationships between age, size, ownership type, and industry sector, and access to green finance. Third-party guarantees were a significant factor in financing through Environment Funds. Moreover, we found commercial environmental projects face fewer green financing obstacles. Surprisingly, showing audited financial statements does not mitigate the information asymmetry between firms and these financial institutions. These findings suggest that Environment Funds should classify environmental project types to develop appropriate lending policies. In emerging markets, enterprises need to build a trusted relationship with financial institutions so that they can replace asset-based lending techniques, thereby increasing the firms' accessibility to green finance.

• Journal of Applied Biological Chemistry
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• 제63권1호
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• pp.75-82
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• 2020

본 연구에서는 한국에서 육종한 신육성품종 그린볼 사과 껍질 추출물(GBE)이 피부 광노화 인자조절에 대한 억제효과를 확인하였다. 피부에서 광노화 인자 조절에 대한 억제 효과를 확인하기 위해 CCD986sk fibrobalst cell에 UVB로 광노화를 유도시킨 후 세포에 GBE를 처리하였다. 광노화 인자 조절 효과를 측정한 결과 GBE가 COL1A2, MMP-1, MMP-9, TIMP-1 단백질 발현량에서 UVB에 의해 자극된 MMP-1, MMP-9 단백질 합성을 억제하였으며, COL1A2, TIMP-1 단백질의 경우 발현량이 유의적으로 증가하는 것을 확인하였다. Photoaging-related factors인 COL1A2, MMP-1, MMP-9, HAS2, TGF-β, TIMP-1의 mRNA 발현량을 측정한 결과 GBE에 의해 MMP-1, MMP-9 단백질 발현을 효과적으로 억제하였으며, MMPs와 type procollagen 발현에 관여하는 조절인자인 TIMP-1과 TGF-β의 발현량이 증가함에 따라 COL1A2의 생성량이 증가하는 것을 확인할 수 있었다. 또한 피부를 구성하는 구조 단백질 중 하나인 hyaluronic acid 생성에 관여하는 HAS2의 발현량 증가도 확인하였다. 따라서 GBE는 광노화의 인자 억제 조절에 대한 우수한 효능을 가졌으며, 피부 노화를 예방하는 기능성 소재로서 활용이 가능할 것으로 판단되었다.

• IMMUNE NETWORK
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• 제12권4호
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• pp.155-164
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• 2012

It is well established that blocking the interaction of EGFR with growth factors leads to the arrest of tumor growth, resulting in tumor cell death. ER414 is a human monoclonal antibody (mAb) derived by guided selection of the mouse mAb A13. The ER414 exhibited a ~17-fold lower affinity and, as a result, lower efficacy of inhibition of the EGF-mediated tyrosine phosphorylation of EGFR when compared with mAb A13 and cetuximab. We performed a stepwise in vitro affinity maturation to improve the affinity of ER414. We obtained a 3D model of ER414 to identify the amino acids in the CDRs that needed to be mutated. Clones were selected from the phage library with randomized amino acids in the CDRs and substitution of amino acids in the HCDR3 and LCDR1 of ER414 led to improved affinity. A clone, H3-14, with a ~20-fold increased affinity, was selected from the HCDR3 randomized library. Then three clones, ER2, ER78 and ER79, were selected from the LCDR1 randomized library based on the H3-14 but did not show further increased affinities compared to that of H3-14. Of the three, ER2 was chosen for further characterization due to its better expression than others. We successfully performed affinity maturation of ER414 and obtained antibodies with a similar affinity as cetuximab. And antibody from an affinity maturation inhibits the EGF-mediated tyrosine phosphorylation of EGFR in a manner similar to cetuximab.

• 한국세라믹학회지
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• 제47권5호
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• pp.457-460
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• 2010

$SmCoO_3$ system was investigated for their application to themoelectric materials. All specimens showed p-type semiconducting behavior and their electrical conductivity ($\sigma$), Seebeck coefficient (S) and power factor were measured at high temperature. And the effect of dopant ions on their thermoelectrical properties were also investigated. $Fe^{3+}$ ion doped into $Co^{3+}$ site enhanced the Seebeck coefficient and decreased the electrical conductivity simultaneously. The maximum Seebeck coefficient value for 60% doping case reached to 780 ${\mu}V$/K at $240^{\circ}C$. However $Fe^{3+}$ doped system cause an negative effect on power factor value. In case of the pure phase, the maximum Seebeck coefficient value reached to 290 ${\mu}V$/K at $240^{\circ}C$ and the maximum electrical conductivity was obtained 748 1/(ohm$\times$cm) at $960^{\circ}C$. As a result, the maximum power factor was obtained $1.49\times10^{-4}$ W/$mK^2$ at $550^{\circ}C$.