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http://dx.doi.org/10.3839/jabc.2020.010

Photo-aging regulation effects of newly bred Green ball apple  

Lee, Eun-Ho (School of Food science & Biotechnology, Kyungpook National University)
Lee, Seung-Yeol (School of Applied Biosciences, Kyungpook National University)
Jung, Hee-Young (School of Applied Biosciences, Kyungpook National University)
Kang, In-Kyu (Department of Horticultural Science, Kyungpook National University)
Ahn, Dong-Hyun (Department of Food Science and Technology, Pukyong National University)
Cho, Young-Je (School of Food science & Biotechnology, Kyungpook National University)
Publication Information
Journal of Applied Biological Chemistry / v.63, no.1, 2020 , pp. 75-82 More about this Journal
Abstract
In this study, extracts from the Green ball apple peel (GBE) and the newly bred green ball apple from Korea showed inhibition effects on photo-aging factor regulation associated with skin aging. To investigate the inhibition effect on photo-aging factor regulation in skin, GBE was treated with UVB to induce photo-aging related factors in CCD986sk fibroblast cells. Photo-aging factor regulation effects showed that GBE inhibited UVB-stimulated matrix metalloproteinase (MMP)-1 and MMP-9 protein synthesis in collagen type I alpha 2 chain (COL1A2), MMP-1, MMP-9, and tissue inhibitors of metalloproteinase (TIMP)-1 protein expression. The expression of COL1A2 and TIMP-1 protein was significantly increased. The mRNA expression levels of COL1A2, MMP-1, MMP-9, hyaluronan synthase (HAS)2, transforming growth factor (TGF)-β, and TIMP-1 were decreased by GBE. The expression of TIMP-1 and TGF-β, which are regulators involved in matrix metalloproteinase and type I procollagen expression, was found to increase with increasing expression of COL1A2. The expression of HAS2, which is involved in the production of hyaluronic acid, one of the structural proteins constituting the skin, was also confirmed. Therefore, GBE showed excellent efficacy against photo-aging factor regulation and could be used as functional material to prevent and treat skin aging.
Keywords
CCD986sk fibroblast cells; Extracts; Green ball; Newly bred apple; Photo-aging;
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