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Profiling of T Cell Receptor β-Chain Complimentary Determining Regions 3 Repertoire in Subarachnoid Hemorrhage Patients Using High-Throughput Sequencing

  • Kim, Bong Jun;Ahn, Jun Hyong;Youn, Dong Hyuk;Jeon, Jin Pyeong
    • Journal of Korean Neurosurgical Society
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    • v.64 no.4
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    • pp.505-513
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    • 2021
  • Objective : The adaptive immune response following subarachnoid hemorrhage (SAH) is not well understood. We evaluated and compared the T cell receptor (TCR) immune repertoire of good-grade and poor-grade SAH patients to elucidate the T cell immunology after ictus. Methods : Peripheral blood from six SAH patients was collected at two different times, admission and at the 7-day follow-up. Composition and variation of the TCR β-chain (TCRB) complimentary determining regions (CDR) 3 repertoire was examined using high-throughput sequencing; the analysis was based on sampling time and disease severity (good vs. poor-grade SAH). Results : Clonality at admission and follow-up were 0.059 (0.037-0.038) and 0.027 (0.014-0.082) (median, 25th-75th percentile). Poor-grade SAH (0.025 [0.011-0.038]) was associated with significantly lower clonality than good-grade SAH (0.095 [0.079-0.101]). Poor-grade SAH patients had higher diversity scores than good-grade SAH patients. CDR length was shorter in good-grade SAH vs. poor-grade SAH. Differences in clonotype distribution were more prominent in TCRBV gene segments than TCRBJ segments. TCRBV19-01/TCRBJ02-04 and TCRBV28-01/TCRBJ02-04 were the most increased and the most decreased V-J pairs in the 7-day follow-up compared to admission in good-grade SAH. The most increased and decreased V-J pairs in poor-grade SAH patients were TCRBV28-01/TCRBJ02-06 and TCRBV30-01/TCRBJ02-04, respectively. Conclusion : The TCRB repertoire is dynamic in nature following SAH. TCRB repertoire may facilitate our understanding of adaptive immune response according to SAH severity.

Correlation between the Korean pork grade system and the amount of pork primal cut estimated with AutoFom III

  • Park, Yunhwan;Ko, Eunyoung;Park, Kwangwook;Woo, Changhyun;Kim, Jaeyoung;Lee, Sanghun;Park, Sanghun;Kim, Yun-a;Park, Gyutae;Choi, Jungseok
    • Journal of Animal Science and Technology
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    • v.64 no.1
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    • pp.135-142
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    • 2022
  • It is impossible to know the amount of pork primal cut by pig carcass grade which is determined only by carcass weight and backfat thickness in the Korean Pig Carcass System. The aim of this study was to investigate the correlation between the pig carcass grade and the amount of pork primal cut estimated with AutoFom III. A total of 419,321 Landrace, Yorkshire, and Duroc (LYD) pigs were graded with the Korean Pig Carcass Grade System. Amounts of belly, neck, loin, tenderloin, spare ribs, shoulder, and ham were estimated with AutoFom III. Regression equations for seven primal cuts according to each grade were derived. There were significant differences among the three carcass grades due to heteroscedasticity variance (p < 0.0001). Three regression equations were derived from AutoFom III estimation of primal cuts according to carcass grades. The coefficient of determination of the regression equation was 0.941 for grade 1+, 0.982 for grade 1, and 0.993 for grade 2. Regression equations obtained from this study are suitable for AutoFom III software, a useful tool for the analysis of each pig carcass grade in the Korean Pig Carcass Grade System. The high reliability of predicting the amount of primal cut with AutoFom III is advantageous for the management of slaughterhouses to optimize their product sorting in Korea.

Exploring Learning Styles and Task Preferences of Disadvantaged Gifted Students (학년과 성에 따른 소외 영재의 학습 스타일과 과제선호도 탐색)

  • Lee, Mi-Soon
    • Journal of Fisheries and Marine Sciences Education
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    • v.26 no.2
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    • pp.296-307
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    • 2014
  • As an educational trial for pursuit of educational excellence in the disadvantaged gifted, this study was to explore learning styles and task preferences by student's grade and gender. Furthermore, this study sought to present the practical basis to develop programs for disadvantaged gifted students. Total 153 disadvantage gifted students responded items of the Learning Styles Inventory-III and the Task Preferences Scale, which responses were analyzed by student's grade and gender in using MANOVA. As the results, the 1st grade disadvantaged gifted students preferred Direct instruction, Technology, and Learning games to the higher grade level students. There were significant differences in task preferences by students' grade level. The 4th grade disadvantaged gifted students preferred Creative tasks and Difficult tasks more than other grade level students.

Daily Hassles, Bullying and Victimization : A Comparison of Grade and Gender (일상적 스트레스와 또래 괴롭힘의 가해와 피해경험 : 학년과 성별 비교)

  • Kim, Gill Im;Sim, Hee Og
    • Korean Journal of Child Studies
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    • v.23 no.5
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    • pp.139-151
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    • 2002
  • This study explored the conditions of daily hassles and the relations between daily hassles, bullying, and victimization by grade and gender. Data were collected from 530 1st-6th grade children through questionnaires. The extent of stress, bullying, and victimization of children differed by grade and gender. Males reported higher parent-, study-, and friend-related stress than females. Bullying and victimization were highly related to each other. Most bullying appeared in 3rd-6th grade children while most victimization occurred in 1st-2nd grade children. Most bully/victims were in 5th-6th grade children. More females than males were not-involved and victimized. More males than females were bullies and bully/victims. Bullies, victims, bully/victims, and not-involved children experienced different degrees of daily hassles.

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A Study of High Particle Collection Efficiency of Cyclone (고효율 사이클론의 집진효율에 관한 연구)

  • Chung, Jin-Do;Lee, Sang-Gwon;Bohnet, M.
    • Proceedings of the KSME Conference
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    • 2003.04a
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    • pp.2227-2232
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    • 2003
  • For a reasonable analysis of grade efficiency of cyclone separator, in-line measurements of particle size and particle concentration are needed. The purpose of this study is to define the correlation of measurement data and to measure grade efficiency and pressure drop. The authors adapted that the grade efficiency could be calculated directly from the counted particle numbers. The problem of collection efficiency calculation was different from counting rate of aerosol counters under the same conditions. It is important factor to use the rate of the particle counters to obtain reliable results. Therefore, the authors should use and calculate compensation factor considering sensitivity of aerosol counters, gas velocity and concentration affect higher 30% than originally measured grade efficiency. As the pressure drop and grade efficiency increase, as flow rate increase and then also pressure drop and grade efficiency increase.

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Development & Application of Ni free 4Y grade FCW specified by class society (Ni free 선급 규정 4Y grade 플럭스코어드 와이어 개발, 적용)

  • Nam Seong-Gil;Jang Tae-Won;Yun Dong-Ryeol;Han Jeong-Seok;Seon Hye-Seon
    • Proceedings of the KWS Conference
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    • 2006.05a
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    • pp.133-134
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    • 2006
  • The welding consumables which are able to be applied in shipbuilding is decided in accordance with base materials. The class societies regulate that 3Y grade welding consumables have to be used for E(H) grade steel. Welding consumables with about 1.5% Ni contents in the deposited metal have been using to weld E(H) grade steel in domestic shipbuilding companies. It was inevitable to obtain impact property of weldment at low temperature. However, when the welding consumables are used in production, there are two kinds of problems-bad workability & cost-up of welding consumables. Therefore development of Ni free welding consumables for E(H) grade steel are required & this paper will introduce the development of Ni free 4Y grade FCW.

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Validation of School Adjustment Inventory for First Grade Elementary School Students (초등학교 일학년용 학교적응 척도 타당화 연구)

  • Chi, Sung-Ae;Jung, Dae-Hyun
    • Korean Journal of Child Studies
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    • v.27 no.1
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    • pp.1-15
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    • 2006
  • The purpose of this study was to examine the reliability and validity of the School Adjustment Inventory for 1st grade elementary school students(SAI-1st Grade). The subjects of this study were 624 first grade children attending 40 elementary schools and 120 first grade teachers of G City. Data were analyzed by Factor Analysis(EFA), Confirmatory Factor Analysis(CFA), Pearson's r and Cronbach's ${\alpha}$. Results of the test on content validity as evaluated by the first grade teachers was high. It was found that the School Adjustment Inventory for 1st graders of elementary school was a reliable and valid measure for evaluating the school adjustment of first grade students.

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A Study on the Relationship of Children's Development and Book Preference (아동의 심리발달과 도서선호의 변화)

  • Han Yoon Ok
    • Journal of the Korean Society for Library and Information Science
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    • v.17
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    • pp.105-159
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    • 1989
  • The purpose of this study is (a) to identify physical and content elements of children's books that seem to interest second and fifth graders to read, and (b) to observe how the influence of those elements on children's reading interest shifts as children develop. For the purpose of this study, children's books are classified into two groups: 'books of low preference' and 'books of high preference' as determined by second graders, and fifth graders' library circulation record in the library chosen as the sample for the present study. The physical and content elements of the books belonging to these two groups are analyzed and compared. Main findings and conclusions made in this study are summarised as follows: (1) The physical elements of children's books which have significant relationship with second graders' reading preference are illustration quantity, size of types, space between lines, and size of the book. (2) The content elements of children's books which have significant relationship with second graders' reading preference are the readability level of sentences, and the sex of heroes. (3) The physical elements of children's books which have significant relationship with fifth graders' reading preference are illustration quantity, illustration styles, and size of the book. (4) The content elements of children's books which have significant relationship with fifth graders' reading preference are the readability level, the sex of heroes, subject matters, themes and the environmental settings as the background of stories. (5) Reading preference of the pupils at the second and fifth grades are influenced by the quantity of illustration. Second grade pupils prefer reading books which contain a larger quantity of illustrations, whereas 5th grade pupils prefer books which contain less quantity of illustrations. (6) The relationship between second graders' reading preference and the illustration style are rejected at the $10\%$ significance level. A strong relationship, however, appears to exist between 5th graders' reading preference and the illustration style. The 5th grade pupils prefer more realistic illustrations, while cartoons and semi-abstract drawings do not appeal to them. (7) The type size, and space between lines which have had great influence on the book preference of 2nd grade pupils influence little on the 5th grade pupils book preference. (8) Second grade pupils prefer the book of a small Size, whereas 5th grade pupils whose reading taste has developed at a primary stage prefer the book of a large size beyond a certain standard. (9) Second grade pupils prefer reading books which can be easily read, whreeas 5th grade pupils prefer reading books containing difficult sentences. (10) Second grade pupils and the 5th grade pupils both prefer books in accordance with the sex of there heroes. (11) Relationship between second graders' reading preference and the subject matters are rejected at the 10% significance level. A strong relationship, however, appears to exist between 5th graders' reading preference and the subject matter. Fifth grade pupils prefer reading books of which the themes are mystery, exploration and adventure. (12) The theme of books is not proved to relate with the book preference of the 2nd grade pupils, but it is closely related with the book preference of 5th grade pupils. Fifth grade pupils prefer reading books which contain exploratory stories. (13) Second grade pupils are not influenced by the environmental setting of the story, but 5th grade pupils prefer reading stories with the environmental setting in the air. (14) The quantity of illustrations is the element influencing most on .the book preference of the 2nd grade pupils, followed by the space between the lines, which influences least on the book preference of the 5th grade pupils. (15) The physical element influencing most on the book preference of the 2nd grade pupils is the quantity of the illustrations. The physical element which has the most strong influence on the book preference of the 5th graders is illustration styles. (16) The content elements which has much influence on the book preference of the 5th grade pupils are certain kinds of characteristics of heroes. In case of the 2nd grade pupils book preference, striking evidence of such characteristics of heroes is not traced.

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ON THE STRUCTURE OF THE GRADE THREE PERFECT IDEALS OF TYPE THREE

  • Choi, Eun-Jeong;Kang, Oh-Jin;Ko, Hyoung-June
    • Communications of the Korean Mathematical Society
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    • v.23 no.4
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    • pp.487-497
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    • 2008
  • Buchsbaum and Eisenbud showed that every Gorenstein ideal of grade 3 is generated by the submaximal order pfaffians of an alternating matrix. In this paper, we describe a method for constructing a class of type 3, grade 3, perfect ideals which are not Gorenstein. We also prove that they are algebraically linked to an even type grade 3 almost complete intersection.