• Korean Journal of Agricultural Science
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• v.8 no.2
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• pp.238-243
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• 1981

This experiment was carried out to study the availability of Korean peat as a main carrier material of rhizobial inoculant, using the alfalfa strain Rhizobium meliloti M 14 which was isolated in the previous report. Modification of powdered peat with calcium carbonate and other materials was studied; inoculation of the peat with culture broth, maturation of the mixture under different conditions, and survival of the strain in the peat culture was examined. The results obtained were as follows. 1. Peat produced in Pyongtak was highly acidic, pH 3.8, and addition of calcium carbonate by 14% was required for pH adjustment to 6.4. However the amount of calcium carbonate could be reduced by 4 to 8% when carbon or charcoal was mixed with the peat. 2. Viable number of the strain reached to $7-9{\times}10^9cells/g$ after 3 days, when inoculated with the culture broth of early stationary growth phase and matured in unsteriled peat of open trays; and the number in steriled peat was $1.1-6.2{\times}10^{10}cells/g$ after 5 days, when matured in closed bottles. 3. Survival of the strain was affected markedly by storage temperature, and positive effect of D-sorbitol on the viability was recognized at elevated temperatures, when added as an additional carbon source and moistening agent. Glycerol, sorbitol, or sodium lactate was utilized by the strain as a sole source of carbon, and the decimal reduction time of viable number in the peat culture was was found to be 8 to 9 weeks at $25^{\circ}C$ when these agents were added by 0.5%.

• Journal of Korean Society of Forest Science
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• v.102 no.4
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• pp.522-529
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• 2013

Climate change will affect the physiological traits and growth of forest trees. This study was conducted to investigate the effects of an experimental warming on growth and physiological characteristics of Pinus densiflora S. et Z. seedlings. One-year-old P. densiflora seedlings were planted in control and warmed plots in April 2010. The air temperature of warmed plots was increased by $3^{\circ}C$ using infrared lamps from November 2010. We measured shoot height, root collar diameter, above and below ground biomass, chlorophyll contents and leaf nitrogen concentration from March 2011 to March 2013. Seedling height and root collar diameter showed no significant difference between warmed and control plots except for root collar diameter measured in June 2012. Seedling leaf biomass was lower in the warmed ($23.94{\pm}2.10g$) than in the control ($26.08{\pm}1.72g$) plots in 2013. Shoot to root ratio (S/R ratio) was lower in the warmed ($1.09{\pm}0.07$) than in the control ($1.31{\pm}0.10$) plots in 2013. Leaf nitrogen concentrations and chlorophyll contents were not significantly different between warmed and control plots except for leaf nitrogen concentration in 2011. Leaf C/N ratio was increased in 2012 under the warming treatment. Low growth and S/R ratio in warmed plots might be related to the higher temperature and water stress. In the future, the below-ground carbon allocation of P. densiflora might be increased by global warming due to temperature and water stress.

• Korean Journal of Weed Science
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• v.3 no.1
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• pp.23-28
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• 1983

To study ecological characteristics of Eleocharis kuroguwai occuring in Korea its propagules were collected from 6 locations from the northern part to the southern part of Korea (Chuncheon, Suweon, Iri, Jeonju, Gwangiu and Milyang) in 1981, cultured and replanted 3 times (May 20, June 5, June 20) in 1982. They flowered from August 5 to August 27 when they were planted on May 20, and from August 20 to August 27 when they were planted on Tune 20. Plant height, number of tillers and top fresh weight/$m^2$ were 50-90cm, 500-875, and 175-750g, respectively when they were planted on May 20, and 40-70cm, 250-625, 325-625g, respectively when they were planted on June 20. Number of tubers per plant were 0.98-1.98 when they were planted on May 20, and 1.81-2.87 when they were planted on June 20. Eleocharis kuroguwai from Chuncheon or Suweon was more open in plant type, shorter in plant height, narrower in diameter of pedicel and shorter in inflorescence than those from Iri, Jeonju or Gwangju. Each of the local collections may be regarded as different ecotype, based on the above differences in morphology and responses in growth and flowering to the planting dates. The results appear to imply that Eleocharis kuroguwai weeds occurring in various locations of Korea are different one another in competitive avility with rice crop.

• Journal of the Korea Organic Resources Recycling Association
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• v.14 no.1
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• pp.112-130
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• 2006

In order to improve the waste management system for the unit of small villages, we visited 14 counties nationwide and investigated the present conditions of the farming and fishing villages on waste management. First, we selected one concentrated residential type village and one separated residential type village. Then we investigated the quantities and the characteristics of the domestic wastes generated from the 2 sample villages that we chose early on. The concentration of 7 heavy metals in open burned residuals was analyzed. The results are as follows: (1) We distributed the 799 questionnaires to public servants (83 Gun, 716 Myon) and 337 questionnaires to residents. In accordance with a result of questionnaire on the Volume-Based Waste Fee System, the rate of participated questionnaire was 70.3% from public servants and 31.8% from residents. (2) From the survey on two chosen villages, the generation of municipal waste was 48.6g/person/day in A Village, 54.3g/person/day in B Village. This amount was smaller than that of national average of 1.05kg because of excluding recyclable wastes and some of incinerated combustible waste. (3) The 14 counties entered the aged society. There were 2 aging societies, 8 aged societies and 4 super-aged societies. And the average ratios of Public financial independence and waste budget of financial independence were 16.1% and 17.9% respectively. These are lower than that of national average of 57.2% and 31.4%. This indicates that these factors seemed to be operated with the hindrance insettling Volume-Based Waste Fee System in the small villages.

• Journal of Bio-Environment Control
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• v.24 no.4
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• pp.296-300
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• 2015

This study was conducted to investigate optimal crop loads of 'Jinok' grape for unheated plastic house culture. The crop loads of 'Jinok' grapes were managed to be 1.3, 1.8, 2.2, 2.4, and 2.6t per 10a from 2012 to 2014. We measured vine growth and berry qualities. Crop loads were not significantly affected on plant height, trunk diameter, shoot length, and the internode length of 'Jinok'. However, the maturation of berries was delayed when the crop load was higher. And the harvest date was earlier about three weeks in an unheated plastic house compared to in an open field. The average berry weight was decreased by the higher crop load although higher crop loads made higher yields showing the lowest weight at 342g with the treatment of 2.6t per 10a and the highest weight at 363g with the treatment of 1.3t per 10a. Also, the soluble solids content showed a tendency that higher crop loads brought to lower degree Brix. The contents of P, K, Ca, and Mg in grape leaves and shoots were not significantly different by crop loads. To sum up, when crop loads were under the 2.4t per 10a, the berries were harvested as a marketable fruit having $15^{\circ}Brix$ in the cultivar 'Jinok' grape. This result could help to increase grower's benefit having improved quality of fruit for the sustainable production by the established cultivation techniques for the newly developed cultivar 'Jinok'.

• Journal of Animal Environmental Science
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• v.2 no.1
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• pp.41-52
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• 1996

This experiment was carried out to study the effect of sprinkler and fan cooling system on the physiological parameter, milk production and milk composition for Holstein cows in hot, humid climates. Thirty cows were assigned to one of two sections of open-sided loose barn. Water nozzles of sprinkler system were spaced in line at 1.2m intervals. Forced air was provided by 85cm diameter fans at rate of 3.4㎥/sec. The results obtained from these experiments are as follows: 1. There was no significant difference in meteorological data between control and fan + sprinkler cooling system(treatment group). 2. Skin temperature and rectal temperature of the treatment group were significantly lower than those of the control group (32.96 : 39.53$^{\circ}C$ vs 34.02 : 41.21$^{\circ}C$ respectively) (P<0.05). 3. Serum cortisol concentration of the treatment group(0.90$\mu\textrm{g}$/dL) was lower than that of control group(1.44$\mu\textrm{g}$/dL)(P<0.05). 4. Milk production of cows cooled with a sprinkler and fan cooling system was significantly higher than that of no cooling system (P<0.0l). 5. Lactose, protein and solid-not-fat content of milk were not changed by the treatments. Milk fat content of the control(3.23%) was low compared with the treatment group(3.38%). Somatic cell count was reduced by 26.63% in the treatment. The results indicate that a sprinkler and fan cooling systems can provide an effective means to relieve heat stress and enhance productivity of lactating Holstein cows during hot and humid summer season.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.27 no.4
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• pp.194-210
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• 2022

The monthly inventory of dissolved inorganic carbon (C_T) and its fluxes were simulated using a box-model for the southeastern Yellow Sea, bordering the northern East China Sea. The monthly C_T data was constructed by combining the observed data representing four seasons with the data adopted from the recent publications. A 2-box-model of the surface and deep layers was used, assuming that the annual C_T inventory was at the steady state and its fluctuations due to the advection in the surface box were negligible. Results of the simulation point out that the monthly C_T inventory variation between the surface and deep box was driven primarily by the mixing flux due to the variation of the mixed layer depth, on the scale of -40~35 mol C m^-2 month^-1. The air to sea CO₂ flux was about 2 mol C m^-2 yr^-1 and was lower than 1/100 of the mixing flux. The biological pump flux estimated magnitude, in the range of 4-5 mol C m^-2 yr^-1, is about half the in situ measurement value reported. The C_T inventory of the water column was maximum in April, when mixing by cooling ceases, and decreases slightly throughout the stratified period. Therefore, the total C_T inventory is larger in the stratified period than that of the mixing period. In order to maintain a steady state, 18 mol C m^-2 yr^-1 (= 216 g C m^-2 yr^-1), the difference between the maximum and minimum monthly CT inventory, should be transported out to the East China Sea. Extrapolating this flux over the entire southern Yellow Sea boundary yields 4 × 10⁹ g C yr^-1. Conceptually this flux is equivalent to the proposed continental shelf pump. Since this flux must go through the vast shelf area of the East China Sea before it joins the open Pacific waters the actual contribution as a continental shelf pump would be significantly lower than reported value. Although errors accompanied the simple box model simulation imposed by the paucity of data and assumptions are considerably large, nevertheless it was possible to constrain the relative contribution among the major fluxes and their range that caused the C_T inventory variations, and was able to suggest recommendations for the future studies.

• Korean Journal of Environmental Agriculture
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• v.32 no.1
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• pp.42-47
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• 2013

BACKGROUND: As improved LEDs (Light Emitting Diode) industry and decreased the price of LEDs in Korea, some farmers try to using the RED LEDs in green house and open field to increase the production of crop under bad weather condition. The aim of this study is to find out the effect of RED and FAR-RED LEDs lighting on the fruit quality of twelve-year old 'Hongro'/M.26 apple during night after sunset. METHODS AND RESULTS: FAR-RED (730nm, 2 and 4 hour) and RED (620nm, 2 and 4 hour) with 20 LED/PCB were treated in orchard for 16 weeks from June 10 to October 10 in 2009 and 2010 with control as an comparison. In our experiments, leaf weight was significantly higher in RED LEDs than control, tended to be decreased as times of FAR-RED lighting increased. Fruit weight was increased more in RED LEDs than control in 2009 and 2010, but decreased in FAR-RED lighting compared to control in 2010. Firmness and Hunter's a value of fruit were increased in FAR-RED lighting with 2 and 4 h than control. Soluble solid contents were higher in 2 h RED and 2, 4 h FAR-RED LEDs compared to control in 2009, there was no significant difference in 2010. Acid contents were no difference among the treatments. CONCLUSION(S): In our results, we found that RED LEDs was more helpful to increase the fruit weight and FAR-RED LEDs promote to be higher hunter a value of fruit skin. So, we thought that it is necessary to more study if mixed of RED and FAR-RED lighting is more helpful to promote fruit quality of 'Hongro' apple than single lighting of RED or FAR-RED LEDs respectively.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.24 no.3
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• pp.185-192
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• 1991

The vertical distribution and chemical characteristics of water masses were measured along two south-north transects in the polar front region of the central Korean East Sea. In February, a thermocline was present at depth between 50m and loom at the southern sites of a landward A-transect, and its depth was gradually deepened northward. At an outside B-transect, a thermocline was observed at significantly deep depth of 300m to 400m at two northern stations(Stn. 10 and 11), though the depth of the southward stations was nearly identical to that at the northward stations on a A-transect. In September, there were vertically more various water masses, i.e. the Tsushima Warm surface water(TWSW) or more than $20^{\circ}C$, the Tsushima Middle water(TMW) with a range of $12{\~}17^{\circ}C$, the North Korea Cold Water(NKCW) with $1{\~}7^{\circ}C$ temperature, the Japan Sea Proper Water(JSPW) of less than $1^{\circ}C$, and the mixed water. The North Korea Cold Water could be distinguishable from the other waters, especially from the mixed water of the Tsushima Middle Water and the Japan Sea Proper Water by the pattern of $T-O_2$ diagram. For instance, the North Korea Cold Water had higher oxygen by $1{\~}2ml/l$ than those in the mixed water, although both the two water masses ranged $1{\~}7^{\circ}C$ in water temperature. AOU value was the highest in the JSPW and the lowest in the TWSW. Also, AOU indicated a nearly linear and negative correlation with water temperature. However, AOU data for two masses, the NKCW and the TMW, in September departed remarkably from a regression line. Moreover, the ratio of $$\Delta P/\Delta AOU)$ in September was about $0.45{\mu}g-at/ml$ and higher than the value observed in the open sea. This high value could be elucidated by two factors; intrusion of the NKCW with high oxygen and molecular diffusion of dissolved oxygen from the surface into the lower layer. AOU would be a useful tracer for water masses in the polar front area of the Korean East Sea.

• Journal of Life Science
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• v.21 no.12
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• pp.1666-1677
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• 2011

The regulation of gene expression plays an important role in cell cycle controls. In this study, a novel gene, the $mas1^+$($\underline{mi}$tosis $\underline{as}$sociated protein) gene, a homolog of human CIP29/Hcc1, was isolated and characterized from fission yeast Schizosaccharomyces pombe (S. pombe) using a gene-specific polymerase chain reaction. The isolated gene contained a complete open reading frame capable of encoding 245 amino acid residues with a typical promoter, as judged by nucleotide sequence analysis. It was also found that a PCB ($\underline{p}$ombe cell $\underline{c}$ycle $\underline{b}$ox) is located in the promoter region, which controls M-$G_1$ specific transcription in S. pombe. The quantitative analysis of the $mas1^+$ transcript against $adh1^+$ showed that the pattern of expression is similar to that of the septation index. Cytokinesis of mas1 mutant was greatly delayed at $25^{\circ}C$ and $36^{\circ}C$, and a large number of multi-septate cells were produced. The mas1 mutant had 2C, 4C and 6C DNA contents, as determined by FACS analysis. In addition, the number of multi-septate cells significantly increased. When cells were cultured in nitrogen starvation medium to increase proliferation, the abnormal phenotypes of mas1 mutant dramatically increased. These phenotypes could be rescued by an overexpression of the $mas1^+$ gene. The mas1 protein localized in the nuclei of S. pombe and human HeLa cells, as evidenced by Mas1-EGFP signals. The abnormal growth pattern and the morphology of mas1 mutant were complemented by a plasmid carrying human CIP29/Hcc-1cDNA. In addition, CIP29 /Hcc-1 transcript level increased in active cell proliferation stages in the developing mouse embryos. These results indicate that the $mas1^+$ ishomologous to the human CIP29/Hcc1 gene and is involved in cytokinesis and cell shape control.