• Korean Journal of Heritage: History & Science
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• v.55 no.2
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• pp.186-197
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• 2022

The Daejanggakgibi Stele of Silleuksa Temple in Yeoju is a stone stele from the Goryeo Dynasty that is inscribed with various stories about the construction of Daejanggak, a place where Buddhist scriptures were kept. This stele has been maintained for a long time in a state in which discoloration of the body has occurred, and the inscription has been partially damaged due to dozens of cracks. Using non-destructive analysis methods for stone artifacts, material investigation, portable X-ray fluorescence analysis, and ultrasonic velocity analysis for the stele were performed. It was confirmed that the stele body was composed of light gray crystalline limestone, and the base stone, support stone, and cover stone were medium-grained biotite granite. Portable X-ray fluorescence analysis confirmed that iron(Fe) was an original coloring element of the stele surface. From the distribution pattern of the coloration, it can be inferred that iron-containing materials flew down from between the stele body and the cover stone. Thereafter, living organisms or organic contaminants attached to it so that yellow and black contaminants were formed. Ultrasonic diagnosis revealed that the physical property of both the front and back surfaces ranged from fresh rocks(FR) to completely weathered rocks(CW), and the average weathering index was grade 3(intermediate). However, the point where cracks developed intensively was judged to be the completely weathered stage(CW), and some cracks located in the upper and lower parts of the stele bear potentially very high risk. It is necessary to monitor the movement of these cracks and establish reinforcement measures for conservation in the future.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.18
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• pp.7849-7855
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• 2014

Purpose: To investigate the effect of deacetylase inhibitory trichostatin A (TSA) on anti HepG2 liver carcinoma cells and explore the underlying mechanisms. Materials and Methods: HepG2 cells exposed to different concentrations of TSA for 24, 48, or 72h were examined for cell growth inhibition using CCK8, changes in cell cycle distribution with flow cytometry, cell apoptosis with annexin V-FTIC/PI double staining, and cell morphology changes under an inverted microscope. Expression of ${\beta}$-catenin, HDAC1, HDAC3, H3K9, CyclinD1 and Bax proteins was tested by Western blotting. Gene expression for ${\beta}$-catenin, HDAC1and HDAC3 was tested by q-PCR. ${\beta}$-catenin and H3K9 proteins were also tested by immunofluorescence. Activity of Renilla luciferase (pTCF/LEF-luc) was assessed using the Luciferase Reporter Assay system reagent. The activity of total HDACs was detected with a HDACs colorimetric kit. Results: Exposure to TSA caused significant dose-and time-dependent inhibition of HepG2 cell proliferation (p<0.05) and resulted in increased cell percentages in G0/G1 and G2/M phases and decrease in the S phase. The apoptotic index in the control group was $6.22{\pm}0.25%$, which increased to $7.17{\pm}0.20%$ and $18.1{\pm}0.42%$ in the treatment group. Exposure to 250 and 500nmol/L TSA also caused cell morphology changes with numerous floating cells. Expression of ${\beta}$-catenin, H3K9and Bax proteins was significantly increased, expression levels of CyclinD1, HDAC1, HDAC3 were decreased. Expression of ${\beta}$-catenin at the genetic level was significantly increased, with no significant difference in HDAC1and HDAC3 genes. In the cytoplasm, expression of ${\beta}$-catenin fluorescence protein was not obvious changed and in the nucleus, small amounts of green fluorescence were observed. H3K9 fluorescence protein were increased. Expression levels of the transcription factor TCF werealso increased in HepG2 cells following induction by TSA, whikle the activity of total HDACs was decreased. Conclusions: TSA inhibits HDAC activity, promotes histone acetylation, and activates Wnt/${\beta}$-catenin signaling to inhibit proliferation of HepG2 cell, arrest cell cycling and induce apoptosis.

• Journal of Bio-Environment Control
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• v.31 no.3
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• pp.194-203
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• 2022

Cultivation of tomatoes in Korea grown in soil covers 89% of the total area for tomato cultivation. Tomatoes grown in soil often encounter various environment stresses including not only salt stress and soil-borne diseases but also cold stress in the winter season. This study was conducted to comparatively analyze the performance of rootstocks with cold stress by measuring the growth, yield, and photosynthetic efficiency in tomatoes grown in soil. The rootstocks were used 'Powerguard', 'IT173773', and '20LM' for the domestic rootstock cultivars and 'B-blocking' for a control cultivar. The tomato cultivar 'Red250' was used as the scion and the non-grafted tomatoes. Stem diameter, flowering position, leaf length, and leaf width were investigated for the growth parameters. The stem diameter of the non-grafted tomatoes decreased by 15% compared to the grafted tomatoes at 80 days after transplanting when exposed to low temperatures of 9-14℃ for 14 days. The leaf length and width of the non-grafted tomatoes were the lowest with 42.4 cm and 41.8 cm at 80 days after transplanting. The total yield per plant was the highest in tomato plants grafted on 'Powerguard' with 1,615 g and lowest in non-grafted tomatoes with 1,299 g. As the result of measuring the chlorophyll fluorescence parameters, PI_ABS and DI₀/RC, which mean the performance index and dissipated energy flux, 'Powerguard' was the highest with 3.73 in PI_ABS and the lowest with 0.34 in DI₀/RC, whereas non-grafted tomatoes was the lowest with 2.62 in PI_ABS and the highest with 0.41 in DI₀/RC at 80 days after transplanting. The stem diameter has positive correlation with PI_ABS, while it has negative correlation with DI₀/RC. The results indicate that can be analyzed by chlorophyll fluorescence parameters can be used for analyzing the differences in the growth of tomato plants grafted on different rootstocks when exposed to cold stress.

• Korean Journal of Remote Sensing
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• v.39 no.2
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• pp.169-181
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• 2023

Recently, in order to reduce carbon dioxide (CO₂) emissions, which is the main cause of global warming, Korea has declared carbon emission reduction targets and carbon neutral. Accurate assessment of regional emissions and atmospheric CO₂ concentrations is becoming important as a result. In this study, we identified the spatiotemporal differences between satellite-based atmospheric CO₂ concentration and CO₂ emissions for the Korean Peninsula region using column-averaged CO₂ dry-air mole fraction from the Orbiting Carbon Observatory-2 and emission inventory. And we explained these differences using solar-induced fluorescence (SIF), a photosynthetic reaction index according to vegetation growth. The Greenhouse Gas Inventory and Research Center (GIR) and Emissions Database for Global Atmospheric Research (EDGAR) emissions continued to increase in Korea from 2014 to 2018, but the satellite-based atmospheric CO₂ concentration decreased in 2018, respectively. Regionally, GIR and EDGAR emissions increased in 2018 in Gyeonggi-do and Chungcheongbuk-do, but satellite-based CO₂ concentrations decreased for the corresponding years. In addition, the correlation analysis between emissions and satellite-based CO₂ concentration showed a low correlation of 0.22 (GIR) and 0.16 (EDGAR) in Seoul and Gangwon-do. Atmospheric CO₂ concentrations showed a different correlation with SIF by region. In the CO₂-SIF correlation analysis for the growing season (May to September), Seoul and Gyeonggi-do showed a negative correlation coefficient of -0.26, Chungcheongbuk-do and Gangwon-do showed a positive correlation coefficient of 0.46. Therefore, it can be suggested that consideration of the CO₂ absorption process is necessary for analyzing the relationship between the atmospheric CO₂ concentration and emission inventory.

• Korean Journal of Clinical Pharmacy
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• v.1 no.1
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• pp.31-36
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• 1991

Tobramycin is one of the most frequently selected agents for pharmacokinetic drug monitoring because of its narrow therapeutic index and essential role for the management of serious infections, especially gram-negative infections. Its pharmacokinetic parameters are dependent on race, sex, age, ideal body weight. disease states, and etc. Therefore, to schedule the dosing of tobramycin, the individual pharmacokinetic parameters such as half-life and volume of distribution are needed. However, these pharmacokinetic parameters have never been reported in Koreans. The purposes of this study were to evaluate the volume of distribution of tobramycin in cancer patients who had normal renal function, to compare the mean values of Vd reported in the literature, and to compare the measured half-life with the expected half-life based on ABW, LBW, and IBW, respectively. Venous blood samples were collected just before and thirty minutes after dosing during steady state. Serum tobramycin concentrations were determined by $TD_x$ (fluorescence immunoassay). IBW were measured by the method of Devine: and LBW were measured by the method of Hallynck. Creatinine clearances (CLcr) of the patients were estimated using the Cockcroft and Gault equation. Elimination rate constants (kel) were determined using the Welling and Craig equation. Infusion rate (ko), volume of distribution (Vd), and half-life $(t_{1/2})$ were determined using the Saw chuk and Zaske equation. The volume of distribution Was $27\%$ greater than the Schentag's study (0.26 vs 0.33 l/kg), but the half-life was similar to the Levy's study. The predicted half-lives based on IBW were the closest to actual half-lives (1.85 vs 2.01 hr).

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.48 no.2
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• pp.56-60
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• 2016

Neutral or alkaline papermaking provides many advantages in paper strength and processing conditions. It also provides the opportunity of using calcium carbonate fillers in papermaking. These diverse advantages have made almost all paper machines of printing and writing papers run under neutral and alkaline conditions. On the other hand, linerboard machines, which use recycled papers as a raw material, are running under acid conditions using a rosin sizing system. Because the recycled raw materials used by the linerboard industry contain significant amounts of alkaline papers, the linerboard industry has an interest in the possibility of using the neutral or alkaline papermaking opportunity. In this study, the compatibility of the recycled linerboards under neutral or alkaline papermaking conditions was examined by recycling them under various pH conditions. The sizing degree of the papers recycled under neutral or alkaline was significantly lower than that of acid formed papers indicating that during the neutral or alkaline recycling process the rosin sized papers lost their sizing efficiency. Recycling of acid formed linerboards under neutral or alkaline conditions increased the amount of foam, and the foam contained substantial amount of solid materials derived from the acid sizing systems. Use of cationic polyelectrolytes including PEI and poly-DADMAC improved the sizing degree of the recycled papers under neutral and alkaline conditions. PEI decreased the foam generation as well while poly-DADMAC did not show any reducing effect of the foam. These results suggest that PEI forms coordinate bonds with rosin acid and precipitate them onto the surface of recycled fibers, while the reaction products between poly-DADMAC and rosin acid ions still remain water soluble under neutral or alkaline conditions.

• Korean Journal of Veterinary Research
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• v.43 no.4
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• pp.677-681
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• 2003

Currently, both the indirect fluorescent antibody test (IFAT) and enzyme linked immunosorbent assay (ELISA) have been used to detect Neospora caninum antibodies. Several factors such as the buffers, the conjugate, the pattern of fluorescence, and the cross reactivity with other apicornplexan protozoan, may result in poorly correlated data. The present study was undertaken to develop and evaluate the Neospora agglutination test (NAT) for the detection and quantification of IgG antibodies to N. caninum from various animal species. Compared to the ELISA method, the NAT with a cutoff value of 1:512 gave a high index of coincidence (kappa=0.807) and no cross reactivity to Toxoplasma gondii antiserum. Hence, this NAT method, which did not require a species-specific secondary antibody and expensive tools, would be easily available for the detection of antibodies to N. caninllm of various animal species.

• Korean Journal of Optics and Photonics
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• v.21 no.1
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• pp.6-11
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• 2010

Fluorescent photopolymer film was prepared with composition containing acrylate monomer, binder, a visible light sensitive photo initiator, and fluorescent anthracene polymer. A fluorescent grating pattern was inscribed on the photopolymer film using a 2-beam coupling method. A 514 nm laser was coupled to generate a beam-interference pattern. A highly fluorescent diffractive line pattern was formed on the fluorescent photopolymer within 30 sec. of exposure. The fluorescence intensity was highly enhanced in the patterned area, possibly due to the change in the environment of the fluorescent polymers by the photo-polymerization of monomers. Under a photo-mask, a gap electrode pattern was formed of fluorescent gratings with a sub-micron scale, which was matched well to the calculated value ($2.5\;{\mu}m$ and $0.6\;{\mu}m$) based on the refractive index of the photopolymer and beam incident angle ($3.4^{\circ}$, $15^{\circ}$) to the photopolymer surface.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.8
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• pp.4853-4858
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• 2013

Objectives: To investigate whether hypoxia has an effect on regulation of multidrug resistance (MDR) to chemotherapeutic drugs in laryngeal carcinoma cells and explore the role of hypoxia-inducible factor-$1{\alpha}$ (HIF-$1{\alpha}$). Methods: Laryngeal cancer cells were cultured under normoxic and hypoxic conditions. The sensitivity of the cells to multiple drugs and levels of apoptosis induced by paclitaxel were determined by MTT assay and annexin-V/propidium iodide staining analysis, respectively. HIF-$1{\alpha}$ expression was blocked by RNA interference. The expression of HIF-$1{\alpha}$ gene was detected by real-time quantitative RT-PCR and Western blotting. The value of fluorescence intensity of intracellular adriamycin accumulation and retention in cells was evaluated by flow cytometry. Results: The sensitivity to multiple chemotherapy agents and induction of apoptosis by paclitaxel could be reduced by hypoxia (P<0.05). A the same time, the adriamycin releasing index of cells was increased (P<0.05). However, resistance acquisition subject to hypoxia in vitro was suppressed by down-regulating HIF-$1{\alpha}$ expression. Conclusion: HIF-$1{\alpha}$ could be considered as a key regulator for mediating hypoxia-induced MDR in laryngeal cancer cells via inhibition of drug-induced apoptosis and decrease in intracellular drug accumulation.

• CELLMED
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• v.2 no.1
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• pp.11.1-11.7
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• 2012

Stachys tibetica Vatke (Lamiaceae) is an important medicinal plant in the folk medicine of Ladakh, India and Tibet for the treatment of various mental disorders. Infusion and decoction of the whole plant is used as a cup of tea for a severe fever, headaches and to relieve tension. The recent study is aimed to evaluate the preliminary pharmacognostical and phytochemical nature of Stachys tibetica Vatke. The whole plant material was subjected to successive soxhlet extraction with petroleum ether (40 - $60^{\circ}C$), chloroform, ethyl acetate, methanol and finally decocted with water to get the respective extracts. The fluorescence characteristics of the powdered materials were analysed under ultraviolet light and ordinary light. Different physicochemical parameters such as ash value, extractive value, foaming index, pH values, loss on drying and determination of foreign matter were carried out as per WHO guidelines. The total fat, flavonoid, saponin and volatile contents were also determined. Macroscopical studies revealed the authentication of the plant drug. Physicochemical parameters helped to standardize the plant material while preliminary qualitative chemical tests of different extracts showed the presence of Glycosides, Carbohydrates, Phytosterols/triterpenoids, Saponins, Fixed oils, Fats and phenols/tannins. Quantification of the total flavonoids and saponins and contents were determined as $54.66{\pm}0.58mg/g$ and $75.42{\pm}0.48mg/kg$ respectively, while the volatile and fat contents were 6.5% and 0.7% respectively. Results may lay the foundation for the standardization of the drug and discovery of new molecules from S. tibetica for the treatment of various diseases.