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Establishment of Neospora agglutination test for serologic diagnosis of neosporosis  

Kang, Min-Soo (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
Kim, Jae-Hoon (Department of Veterinary Medicine, Cheju National University)
Hwang, Woo-Suk (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
Nam, Ho-Woo (Department of Parasitology and Catholic Institute of Parasitic Diseases, College of Medicine, Catholic University)
Youn, Hee-Jeong (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
Bae, Jong-Hee (Department of Veterinary Medicine, Cheju National University)
Kim, Dae-Yong (College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University)
Publication Information
Korean Journal of Veterinary Research / v.43, no.4, 2003 , pp. 677-681 More about this Journal
Abstract
Currently, both the indirect fluorescent antibody test (IFAT) and enzyme linked immunosorbent assay (ELISA) have been used to detect Neospora caninum antibodies. Several factors such as the buffers, the conjugate, the pattern of fluorescence, and the cross reactivity with other apicornplexan protozoan, may result in poorly correlated data. The present study was undertaken to develop and evaluate the Neospora agglutination test (NAT) for the detection and quantification of IgG antibodies to N. caninum from various animal species. Compared to the ELISA method, the NAT with a cutoff value of 1:512 gave a high index of coincidence (kappa=0.807) and no cross reactivity to Toxoplasma gondii antiserum. Hence, this NAT method, which did not require a species-specific secondary antibody and expensive tools, would be easily available for the detection of antibodies to N. caninllm of various animal species.
Keywords
Neospora caninum; Neospora agglutination test (NAT); serology; Korean dairy cattle; dog;
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