• 적정기술학회지
• /
• 제7권2호
• /
• pp.211-215
• /
• 2021

AIDS 환자는 전 세계에 4,000만 명인 것으로 추산되며, 주로 개발도상국에서 대부분의 환자가 발생하고 있다. AIDS의 원인 바이러스인 HIV는 혈중의 CD4+ T 세포에 감염되어 수개월 내지 10년 간의 잠복기간을 거치면서 CD4+ T 세포를 점점 파괴하여 환자의 면역기능을 저하시킨다. 이렇게 면역력이 약화된 AIDS 환자는 다양한 병증에 의해 사망에 이르게 된다. 현재의 CD4+ T 세포 수를 계측하는 방법은 통상 유세포분석(flow cytometry) 방법에 의해 시행되고 있다. 유세포분석 방법은 정확도가 높다는 장점이 있으나 숙련된 전문 인력이 필요하고 장비가 고가이기때문에 개발도상국에서는 이용하기 어렵다. 본 연구결과로 마이크로 여과기를 통해 소량인 5 ㎕ 혈액으로부터 백혈구를 포획하고 포획된 세포에서 CD4+ T 세포 및 CD8+ T 세포를 분석하여 AIDS를 검사하는 장비를 개발하였다. 현재의 검사 장비보다 저렴하고 고도의 기술이 필요하지 않으며 휴대가 간편한 획기적인 AIDS 검사 장비임을 시사한다.

• 대한세포병리학회지
• /
• 제6권1호
• /
• pp.10-17
• /
• 1995

Anaplastic carcinoma of the thyroid gland is one of the most malignant tumors. Recently, DNA ploidy measured by flow cytometry and image analysis has been suggested as an additional useful indicator of tumor behavior. Studies on the occurrence and clinical significance of DNA aneuploidy in anaplastic carcinoma of the thyroid are rare. In this study, the pattern of DNA ploidy was measured by image analysis on Papanicolaou stained slides in four cases of anaplastic carcinoma and also measured by flow cytometry using paraffin blocks in two cases. In all cases of anaplastic carcinoma, DNA aneuploidy was found by image analaysis. By flow cytometry, one case had a diploid peak and the other case had an aneuploid peak. According to the above results, we conclude that anaplastic carcinoma of the thyroid glands have a high incidence of DNA aneuploidy and image analysis using Papanicolaou stained slides is a useful method in detecting DNA aneuploidy.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권8호
• /
• pp.4671-4673
• /
• 2013

Objectives: To study variation in T lymphocyte subgoups and its clinical significance in non-small cell lung cancer (NSCLC). Methods: Levels of CD3+, CD4+, CD8+, CD4+/CD8+, NK and Treg cells in peripheral blood of NSCLC cases and healthy adults were determined by flow cytometry. Results: CD3+, CD4+ and CD4+/CD8+ ratio and NK cells in NSCLCs were decreased significantly in comparison with the control group (P < 0.01), and decreased with increase in the clinical stage of NSCLC, while CD8+ cells demonstrated no significant change (P > 0.05). Treg cells were significantly more frequent than in the control group (P < 0.01), and increased with the clinical stage of NSCLC. Conclusion: The cellular immune function of the NSCLC patients is lowered. It is important to detect change of T lymphocyte subgroups by flow cytometry for the diagnosis, treatment and prognostic assessment of NSCLC patients.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권5호
• /
• pp.2885-2889
• /
• 2013

Goniothalamin is an active compound extracted from Goniothalamus griffithii, a local plant found in northern Thailand. Goniothalamin inhibits cancer cell growth but is also toxic to normal cells. The aims of this study were to identify the cytotoxic effect of goniothalamin and the mechanism of cell death in human HL-60 and U937 cells. Cytotoxicity was determined by MTT assay and cell cycle profiles were demonstrated by staining with propidium iodide (PI) and flow cytometry. Apoptosis was confirmed by staining with annexin V-FITC/propidium iodide (PI) and flow cytometry. Reduction of mitochondrial transmembrane potential was determined by staining with dihexyloxacarbocyanine iodide and flow cytometry and expression of Smac, caspase-8 and -9 was demonstrated by Western blotting. Goniothalamin inhibited growth of HL-60 and U937 cell lines. An increase of SubG1 phase was found in their cell cycle profiles, indicating apoptosis as the mode of cell death. Apoptosis was confirmed by the flip-flop of phosphatidylserine using annexin V-FITC/PI assay in HL60 and U937 cells in a dose response manner. Furthermore, reduction of mitochondrial transmembrane potential was found in both cell types while expression of caspase-8, -9 and Smac/Diablo was increased in HL-60 cells. Taken together, our results indicate that goniothalamin-treated human leukemic cells undergo apoptosis via intrinsic and extrinsic pathways.

• 약학회지
• /
• 제44권2호
• /
• pp.149-154
• /
• 2000

Flavin mononucleotide (1,4-butanediamine) Pt(II) complex (7FMN) was synthesized and screened anticancer activity [J. Pharm. Soc. Korea 43(6),762-770 (1999)]. 7FMN have good water solubility and moderate anticancer activiy In this paper cell-cycle specificity and nephrotoxicity were studied. Interaction of DNA with cisplatin and synthesized 7FMN was analyzed by flow cytometry and showed G2 arrest in L1210 cell line. It means that cell-cycle on L1210 was inhibit in S phase by cisplatin and 7FMN. In order to biochemically analyze nephrotoxicity of cisplatin and 7FMN, after injecting each agent intraperitoneally, blood was exsanguinated after 6 hours, 1 day, 3 days and 7 days, respectively. Then, serum was separated from the blood. The serum level of BUN, creatinine and uric acid in cisplatin and 7FMN administated mice (25~35 g, ICR strain, a dose each 8,12 and 16 times of the $IC_{50}$/ value, cisplatin; 7 times) were determined by autochemistry analyzer. In cisplatinadministered mice group, BUN level was elevated than normal control group at 3rd day and repaired at 7th day. In 7FMN administrated group was not elevated. Creatinine and uric acid level were no difference with the normal control group. Therefore synthesized 7FMN is less toxic than cisplatin in nephrotoxiciaty.

• International Journal of Oral Biology
• /
• 제30권2호
• /
• pp.65-69
• /
• 2005

Periodontitis is a multi-microbial disease and the comparison of a series of periodontopathogenic and non-periodontopathogenic bacteria in terms of microbe-host interaction may provide clues to understand the microbial etiology of the disease better. When we deal with twenty different bacterial species in a study, the first technical issue is how to measure the accurate concentration and use the same number of bacterial cells. We measured bacterial concentration by enumerating bacteria stained with SYTOX green for constant time using a flow cytometer and compared the results with those obtained by plate counting. Concentrations calculated by two different methods were very close. Therefore, flow cytometric counting allowed the rapid analysis of live/dead bacteria, offering the advantage of turbidity measurement and that of colony counting together.

• 한국식품과학회지
• /
• 제38권2호
• /
• pp.290-294
• /
• 2006

본 연구에서는 위생제 처리에 의해 식품에 존재하는 식중독성미생물의 불활성화 효과를 신속하고 직접적으로 평가하는 방법을 개발하고자 하였다. 이는 위생재 처리에 의한 식중독성 미생물의 불활성화 효과를 기존의 plate count 방법으로는 많은 시간이 소요되며, 또한 균주의 특성 및 배양환경과 같은 변수 때문에 정확하게 분석하기에 어렵다는 문제점을 해결하기 위한 방안을 제시하기 위해 진행되었다. 먼저 Salmonella균은 계육 표면의 주로 모공 또는 표면의 주름진 틈사이에 오염되어 존재하는 것으로 확인할 수 있었다. 그리고 TSP 처리에 의한 Salmonellar균의 불활성화 효과를 CLSM과 flow cytometry의 다색 영상화 방법을이용하여 cell viability 염색 방법으로 평가할 수 있었다. 또한 이와 같은 방법을 이용함으로써 Salmonellar균으로 오염된 계육에 TSP 처리하였을 때, Salmonellar균이 계육의 오염부위에서 불활성화 되어 있음을 확인할 수 있었다. 이와 같은 방법으로 식품에 존재하는 식중독성 미생물에 대하여 다양한 위생제 처리에 의한 불활성화 효과를 cell viability 측면에서 직접적이면서 신속하고 명확하게 평가할 수 있음이 확인되었다고 판단된다.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
• /
• pp.151-154
• /
• 2000

배큘로바이러스와 actinomycin D에 의해 유도되는 곤충세포 apoptosis의 누에체액에 의한 저해 효과에 대해 연구하였다. 감염 전 또는 감염 중, 배양 배지에 누에체액을 첨가함으로써 배큘로바이러스에 의해 감염된 숙주 세포의 생존율을 높은 수준으로 유지시켜 주었다. 누에체액은 또한, RNA 합성 저해제인 actinomycin D에 의해 유도된 apoptosis 역시 저해시켰다. 이러한 효과들은 TUNEL assay와 flow cytometry로 확인하였다. 추가적으로, 누에체액은 감염 후 세포 내 유전자의 발현에는 영향을 미치지 않으나 바이러스 유전자의 발현을 증진시킴을 알 수 있었다.

• 한국어업기술학회:학술대회논문집
• /
• 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
• /
• pp.369-369
• /
• 2003

Hemocytes in marine bivalves play important immunological roles in discrimination, opsonization and phagocytosis of foreign materials as a defense mechanism. In this study we report the flow cytometric implications to investigate the immune parameters such as the compositional and the functional characteristics of hemocytes isolated from the Manila clams, Ruditapes philippinarum. Heterogeneity of the hemocytic cell population was determined by the forward scatter (FSC) and side scatter (SSC) cytometric profile which showed three populations: granulocytes, hyalinocytes and small agranular cells. In addition, phagocytosis rate was measured after adding fluorescent-labeled particles. The data were initially analysed for two-parameters: FSC and SSC, then the fluorescent (FL 1) frequency distribution histogram of the hemocyte population was subsequently obtained.

• Bulletin of the Korean Chemical Society
• /
• 제35권1호
• /
• pp.123-128
• /
• 2014

In this study, we have investigated the flow rate effects of Ag nanoparticle (NP) suspensions on the progress of the cell cycle by using a microfluidic image cytometry (${\mu}FIC$)-based approach. Compared with the conventional "static" exposure conditions, enhancements in G2 phase arrest were observed for the cells under continuously flowing "dynamic" exposure conditions. The "dynamic" exposure conditions, which mimic in vivo systems, induced an enhanced cytotoxicity by accelerating G2 phase arrest and subsequent apoptosis processes. Moreover, we have also shown that the increases in delivered NP dose due to the continuous supply of Ag NPs contributed dominantly to the enhanced cytotoxicity observed under the "dynamic" exposure conditions, while the shear stress caused by these slowly flowing fluids (i.e., flow rates of 6 and $30{\mu}L/h$) had only a minor influence on the observed enhancement in cytotoxicity.