Browse > Article

Assessment of Inactivation for Salmonella spp. on Chicken Meat using Confocal Laser Microscopy and Flow Cytometry  

Jang, Keum-Il (Department of Food Science and Technology, Chungbuk National University)
Chung, Duck-Hwa (Division of Applied Life Science, Gyeongsang National University)
Ha, Sang-Do (Department of Food Science and Technology, Chung-Ang University)
Kim, Keun-Sung (Department of Food Science and Technology, Chung-Ang University)
Lee, Kyu-Ho (Department of Environmental Engineering and Biotechnology, Hankuk Univ. of Foreign Studies)
Kim, Min-Gon (Laboratory of Integrative Biotechnology, Korea research Institute of Bioscience and Biotechnology)
Kim, Cheorl-Ho (Department of Biological sciences, College of Natural Science, Sungkyunkwan University)
Kim, Kwang-Yup (Department of Food Science and Technology, Chungbuk National University)
Publication Information
Korean Journal of Food Science and Technology / v.38, no.2, 2006 , pp. 290-294 More about this Journal
Abstract
Inactivation rates of Salmonella enteritidis in vitro and in vivo were assessed using confocal microscopy and flow cytometry. S. enteritidis was inactivated with 1% (w/v) trisodium phosphate (TSP) and live cells, and inactive cells were distinguished by staining with fluorescent probe, LIVE/DEAD BacLight Bacteria Viability stain. After TSP treatment for 1 min, most of Salmonella cells changed from green (live cells) fluorescence to red (inactive cells) fluorescence, indication of effective sanitizing. Inactivation efficiency and contamination sites of S. enteritidis on chicken skin by TSP treatment were assessed using confocal laser microscopy. Precise flow cytometry histograms for viability changes of S. enteritidis. after TSP treatments were obtained. Efficiency of various sanitizer treatments on foodborne pathogens could be assessed using this method.
Keywords
Salmonella enteritidis; confocal microscopy; flow cytometry; assessment; trisodium phosphate;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Lillard HS. Role of fimbriae and flagella in the attachment of Salmonella typhimurium to poultry skin. J. Food Sci. 51: 54-56 (1986)   DOI
2 Dickson JS, Anderson ME. Microbiological decontamination of food animal carcasses by washing and sanitizing systems: a review. J. Food Prot. 55: 133-140 (1992)   DOI
3 Park DL, Rua SM Jr, Acker RF. Direct application of a new hypochlorite sanitizer for reducing bacerial contamination on foods. J. Food Prot. 54: 960-965 (1991)   DOI
4 Wang H. Slavik MF. Bacterial penetration into eggs washed with various chemicals and stored at different temperatures and times. J. Food Prot. 61: 276-279 (1998)   DOI
5 Fu AH, Sebranek JG, Murano E, Microbial and quality characteristics of pork cuts from carcasses treated with sanitizing sprays. J. Food Sci. 59: 306-309 (1994)   DOI   ScienceOn
6 Bell KY, Cutter CN, Sumner SS. Reduction of foodborne microorganisms on beef carcass tissue using acetic acid. sodium bicarbonate. and hydrogen peroxide spray washes. Food Microbiol. 14: 439-448 (1997)   DOI   ScienceOn
7 Marsh P, Morris NZ, Wellington EMH, Quantitative molecular detection of Salmonella typhimurium in soil and demonstration of persistence of an active but non-culturable population. FEMS Microbiol. Ecol. 27: 351-363 (1998)   DOI
8 Oliver JD, Oagher M, Linden K. Induction of Escherichia coli and Salmonella typhimurium into the viable but nonculturable state following chlorination of wastewater. J. Water Health 3: 249-257 (2005)   DOI
9 Wong Liong JW, Frank JF, Baily S. Visualization of eggshell membranes and their interaction with Salmonella enteritidis using confocal scanning laser microscopy. J. Food Prot. 60: 1022-1028 (1997)   DOI
10 Dickson JS, Koohmaraie M. Cell surface charge characteristics and their relationship to bacterial attachment to meat surface. Appl. Environ. Microbiol. 55: 832-836 (1989)
11 Turner K, Porter J, Pickup R, Edwards C. Changes in viability and macromolecular content of long-term batch culture of Salmonella typhimurium measured by flow cytometry. J. Appl. Microbiol. 89: 90-99 (2000)   DOI   ScienceOn
12 Chung KT, Dickson JS, Crouse JD. Attachment and proliferation of bacteria on meat. J. Food Prot. 52: 173-177 (1989)   DOI
13 Jang KI, Jeong HS, Kim CH, Kim KY. Assessment of inactivation for Campylobacter spp. attached on chicken meat. Korean J. Microbiol. Biotechnol. 33: 302-307 (2005)   과학기술학회마을
14 Muriana PM. Effect of pH and hydrogen peroxide on heat inactivation of Salmonella and Listeria in egg white. Food Microbiol, 14: 11-19 (1997)   DOI   ScienceOn
15 Kim CR, Kim KH, Moon SJ, Kim YJ, Lee YK. Microbiological and physical quality of refrigerated chicken legs treated with acetic acid. Food Sci. Biotechnol. 7: 13-17 (1998)
16 Chung KT, Dickson JS, Crouse JD, Effects of nisin on growth of bacteria attached to meat. Appl. Environ. Microbiol. 55: 1329-1333 (1989)
17 Oh JS, Shin KS, Yoon YD, Park JM. Prevalence of Campylobacter jejuni in broilers and chicken processing plants. Korean J. Food Hyg. 3: 27-36 (1988)
18 Marriott NG. Meat and Poultry Processing and Product Sanitation, pp. 230-264. In: Principles of Food Sanitation 3ed, Chapman and Hall, New York, USA (1994)
19 Krysiski EP, Brown LJ. Marchisello TJ. Effect of cleaners and sanitizers on Listeria monocytogenes attached to product contact surfaces. J. Food Prot. 55: 246-251 (1992)   DOI
20 Ren TJ, Frank JF. Susceptibility of starved planktonic and biofilm Literia monocytogenes to quaternary ammonium sanitizer as determined by direct viable and agar plate counts. J. Food Prot. 56: 573-576 (1993)   DOI
21 Breen PJ, Salari H, Compadre CM. Elimination of Salmonella contamination from poultry tissue by Cetylpyridinium chloride solutions. J. Food Prot. 60: 1019-1021 (1997)   DOI
22 Aveery SM, Buncic S. Antilisterial effects of a sorbate-nisin combination in vitro and on packaged beef at refrigeration temperature. J. Food Prot. 6: 1075-1080 (1997)
23 Mosteller TM, Bishop JR. Sanitizer efficacy against attached bacteria in a milk biofilm. J. Food Prot. 56: 34-41 (1993)   DOI
24 Vodovotz Y, Vittadini E, Coupland J, McClements DJ, Chinavhoti P. Briging the gap: use of confocal microscopy in food research. Food Technol. 50: 74-82 (1996)
25 Vignolo G, Fadda S, Kairuz MND, Holgado APDR. Oliver, G. Effects of curing additives on the control of Listeria monocytogenes by lactocin 705 in meat slurry. Food Microbiol. 15: 259-264 (1998)   DOI   ScienceOn
26 Cho JC, Kim SJ. Viable, but non-culturable state of a green fluorescent protein-tagged environmental isolate of Salmonella typhi in groundwater and pond water. FEMS Microbiol. Lett. 170: 257-264 (1999)   DOI
27 Slavik MF, Kim JW, Walker JT. Reduction of Salmonella and Campylobacter on chicken carcasses by changing scalding temperature, J. Food Prot. 58: 689-691 (1995)   DOI
28 Cutter CN, Siragusa GR, Reductions of Listera innocua and Brochhothrix thermosphacta on beef following nisin spray treatments and vacuum packaging. Food Microbiol. 13: 23-33 (1996)   DOI   ScienceOn
29 Carter NP, Meyer EW. Chapter I. Introduction to the principles of flow cytometry. pp. 1-28. In: Flow cytometry. Ormerod MG (ed). IRL Press, Oxford, NY, USA (1990)
30 Gorman BM, Sofos JN, Morgan JB, Schmidt GR, Smith GC. Evaluation of hand-trimming, various sanitizing agents, and hot water spray-washing as decontamination interventions for beef brisket adipose tissue. J. Food Prot. 58: 899-907 (1995)   DOI
31 Seong HS, Inactivation of Listeria monocytogenes in poultry meat. MS thesis, Chungbuk National University, Chungju, Korea. (2001)
32 Xiong H. Li Y, Slavik MF. Walker JT. Spraying chicken skin with selected chemicals to reduce attached Salmonella typhimurium. J. Food Prot. 61: 272-275 (1998)   DOI
33 Tamblyn KC, Conner DE. Bcatericidal activity of organic acids in combination with transdermal compounds against Salmonella typhimurium attached to broiler skin. Food Microbiol. 14: 477-484 (1997)   DOI   ScienceOn
34 Kim CR, Kim KH. Physicochemical quality and gram negative bacteria in refrigerated chicken legs treated with trisodium phosphate and acetic acid. Food Sci. Biotechnol. 9: 218-221 (2000)
35 Kim KY, Frank JF, Craven SE. Three-dimensional visualization of Salmonella attachment to poultry skin using confocal scanning laser microscopy. Lett. Appl. Microbiol. 22: 280-282 (1996)   DOI   ScienceOn
36 Haugland RP. Chapter 16. Assays for cell viability, proliferation and function. pp. 365-398. In: Handbook of fluoresecent probe and research chemicals, 6th ed, Spence MTZ (ed). Molecular Probes. OR, USA (1996)
37 Hwang CA, Beuchat LR. Efficacy of selected chemical for killing pathogenic and spoilage microorganisms on chicken skin. J. Food Prot. 58: 19-23 (1995)   DOI
38 Weaver JL. Estimation of cell viability by flow cytometry. pp. 77-83. In: Flow cytometry protocols. Jaroszeski MJ, Heller R (eds). Human Press, NJ, USA (1998)
39 Thomas CJ, McMeekin TA. Effect of water uptake by poultry tissues on contamination by bacteria during immersion in bacterial suspensions. J. Food Prot. 47: 398-402 (1984)   DOI