• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2009년도 심포지엄 및 춘계학술발표회
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• pp.397-398
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• 2009

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2008년도 추계학술발표회
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• pp.402-403
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• 2008

• 대한본초학회지
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• 제29권6호
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• pp.165-173
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• 2014

Objectives : Platycodon Root is frequently used in traditional medicine to treat inflammatory diseases of the throat. The purpose of this study was to characterize the effect of the EtOH extract of fermented Platycodon grandiflorum on the ameliorative effects on the Antipruritic Effect of atopic dermatitis mouse model induced by compound 48/80 and ovalbumin (OVA)-induced allergic responses in mice. Methods : In the present study, we examined the anti allergic effect of Platycodon grandiflorum (PR) and its fermented production (FPR) in several mouse model. We measured acute ear edema in a mouse model caused by TPA and consecutively histological change of Ear tissue was observed by hematoxylin and eosin (H&E) staining. and also Scratching behaviors by compound 48/80 was investigated. The levels of allergic mediators such as immunoglobulin (Ig) E, and anti-oxidant markers such as SOD and MDA in the sera of OVA induced allergic mice were measured by enzyme-linked immunosorbent assay. Results : FPR inhibited compoud 48/80-induced scratching behavior in mice, as well as acetic acid-induced writhing in mice. The anti-scratching behavioral effect of FPR was more potent than PR. FPR extract significantly decreased the serum levels of IgE and MDA compared with those of OVA control group. Conclusions : These results indicate that Anti allergic effect of Platycodon grandiflorum is enhanced by fermentation with Saccharomyces cerevisae and FPR may be useful for protection from the itching reactions, which are IgE-mediated representative skin allergic diseases.

• 한국약용작물학회지
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• 제19권6호
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• pp.446-455
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• 2011

Galgeun-tang (GGT) is a traditional medicinal formula that is widely prescribed to treat cold, asthma, and hives in Korea. Fermented herbal medicines can be made more effective than normal herbal medicines by increasing the absorption and bioavailability of the active compounds. In this study, we fermented Galgeun-tang to produce bioconversion constituents using Lactobacillus plantarum (GGT144), and found that four peaks were decreased, three peaks were increased and two new peaks appeared in the HPLC-DAD chromatogram. After HPLC-DAD-guided fractionation of the newly-appearing compounds (1 and 5) and the increased (6, 7, and 9) compounds, the structure of the compounds was determined using NMR and MS. Using this approach the compounds were identified to be pyrogallol (1), daidzein (5), liquiritigenin (6), cinnamyl alcohol (7), and formononetin (9), respectively. In addition, the decreased compounds were identified to be daidzin (2), liquiritin (3), ononin (4), and cinnam aldehyde (8) using HPLC-DAD analysis with standard compounds. The high performance liquid chromatography method was used to quantify the nine constituents in GGT and GGT144. All calibration curves of the standard compounds displayed excellent linearity with a $R^2$ > 0.9968.

• 한국미생물·생명공학회지
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• 제36권3호
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• pp.201-208
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• 2008

장수상황버섯(Phellinus baumii) 균사체를 이용한 한약재의 고체발효를 통한 기능성 강화 한약재 생산을 목적으로, 항산화 활성 또는 혈류 개선활성이 우수하다고 알려진 36종의 한약재를 멸균 후, 장수상황버섯 균사체를 접종하여 고체발효 가능성을 검토하였다. $2^{\circ}C$, 상대습도 90% 배양기에서 20일간 발효시킨 결과, 감국, 대추, 백부자, 신이 및 황정을 제외한 31종에서 양호한 발효가 진행되었으며, 발효 가능여부는 한약재 식물체의 사용부위의 구조적 견고성, 영양조건 및 항진균 활성 등과 연관되리라 판단된다. 한약재의 고체발효 이후, 발효전과 비교하였을 때 평균 수분함량은 $30.84{\pm}15.67%$에서 $67.21{\pm}11.43%$로 2.2배 증대되었으며, 평균 메탄올 추출효율과 평균 pH는 $13.91{\pm}12.22%$ 및 $5.06{\pm}0.87$에서 각각 $11.16{\pm}7.06%$ 및 $4.83{\pm}0.73$ 다소 감소하는 경향을 나타내었다. 각각의 메탄올 추출물을 이용한 항혈전 활성 및 항산화 활성을 측정한 결과, 골쇄보, 천련자, 도엽, 와송의 경우에는 트롬빈 저해 활성이 각각 발효전에 비하여 31.38, 23.63, 22.57, 13.85% 증가되었으며, 원지, 현삼, 백지, 향부자, 골쇄보 및 육종용에서는 DPPH 소거능이 발효전에 비해 각각 58.9, 49.8, 48.1, 32.8, 24.1, 20.7% 증가되어 장수상황버섯 균사체 발효 한약재의 긍정적 생리활성 증가를 확인하였다. 본 연구결과는 상황버섯 균사체를 이용한 한약재의 고체발효를 통해, 특정 기능성의 강화 원재료를 생산할 수 있으며, 골쇄보의 경우와 같이 항혈전 활성 및 항산화 활성이 동시에 증대된 한약재 생산이 가능함을 제시하고 있다.

• 한국식품과학회지
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• 제44권3호
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• pp.367-372
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• 2012

5종의 약용식물을 첨가한 발효홍삼(FRGM) 추출물의 항산화활성 및 ${\alpha}$-glucosidase 저해 활성을 in vitro에서 검토하였고, 또한 STZ로 당뇨를 유발시킨 흰쥐에서 FRGM 추출물의 효능을 살펴보았다. 그 결과, FRGM 추출물은 총 폴리페놀 및 총 플라보노이드 함량이 각각 $22.41{\pm}3.51{\mu}g/mg$, $16.80{\pm}4.22{\mu}g/mg$으로 존재하였고, DPPH 소거 활성을 가지고($RC_{50}=95.57{\pm}7.40{\mu}g/mL$), 또한 높은 ${\alpha}$-glucosidase 저해 활성을 나타내었다. STZ 투여에 의해 증가된 혈청 내 ALT와 AST의 활성이 FRGM 추출물의 투여로 감소하였으며, 간 조직중의 글루타치온 함량은 STZ 투여군에서는 감소되었다가 FRGM 투여시 유의적인 증가를 보였고, 지질과산화물 함량은 STZ 투여시 증가되었다가 FRGM 추출물의 투여시 유의적으로 감소되었다. 또한 간 조직중의 SOD, CAT, GP 그리고 GR의 활성도 STZ 투여로 유의적으로 감소되었다가, FRGM 추출물의 투여로 이들 항산화효소 활성이 유의적으로 증가하였다.

• 생명과학회지
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• 제20권8호
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• pp.1268-1275
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• 2010

본 연구에서는, 장수상황버섯(Phellinus baumii) 균사체를 이용한 한약재 고체발효 추출물의 암세포 성장억제 효과를 확인하고자 36종의 한약재 및 이들의 고체발효 한약재 추출물을 대상으로, 인간 대장암세포(HCT116) 성장억제능, 정상세포(HEK-293, 3T3-L1 및MC3T3-E1)에 대한 세포독성 및 인간 적혈구 용혈활성을 평가하였다. 선별된 36종의 한약재 추출물은 100 ${\mu}g/ml$ 농도에서, 사인, 천궁, 석곡, 백선피, 시체, 두충, 백과, 신이, 와송, 삼칠, 견우자, 원지 및 괄루인의 13종에서 암세포 성장률이 50% 이하를 나타내었으며, 균사체 추출물은 46.3%의 성장률을 나타내었다. 반면 25종의 발효한약재 추출물은 대부분 암세포 성장억제능의 변화가 미미하였으나, 백선피, 백과, 신이, 황부자, 산약, 현삼 및 괄루인은 암세포 증식억제능이 유의적으로 감소하였으며, 도인, 골쇄보, 구기자 및 패모에서는 암세포 성장억제능이 유의적으로 증가하였다. 특히 골쇄보, 구기자 및 패모 추출물의 대장암세포에 대한 $IC_{50}$는 각각 394, 917 및 149 ${\mu}g/ml$이었으나, 발효 후 각각 28, 85 및 80 ${\mu}g/ml$를 나타내었다. 또한 발효 골쇄보, 구기자 및 패모 추출물은 200 ${\mu}g/ml$ 농도까지 정상세포에서 미미한 세포독성을 나타내었다. 이러한 결과는 장수상황버섯 균사체를 이용한 한약재 고체발효가 새로운 항암 활성물질 개발 및 신규의 생리활성물질 생산에 이용될 수 있음을 제시하고 있다.

• 대한의생명과학회지
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• 제24권3호
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• pp.280-284
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• 2018

The incidence of cardiovascular diseases (CVDs) is increasing rapidly in developed countries, with CVDs now representing the leading cause of morbidity and mortality. Natural products and ethnomedicines have been shown to reduce the risk of CVDs. Garlic is a medicinal plant used throughout the world for its anti-inflammatory, antioxidant, and antiplatelet activities. Chitosan is a natural polysaccharide obtained from chitin, and derivatives of chitosan have been shown to inhibit platelet aggregation and adhesion. We hypothesized that fermented preparations of these products may possess stronger antiplatelet effects than the non-fermented forms owing to the increased bioavailability of the bioactive compounds produced during fermentation. Therefore, we compared these compounds via in vitro and ex vivo platelet aggregation assays by using standard light transmission aggregometry and ex vivo granule secretions from rat platelets. We found that fermented preparations exerted more potent and significant inhibition of platelet aggregation both in vitro and ex vivo. Likewise, ATP release from dense granules of platelets was also significantly inhibited in fermented preparation-treated rat platelets compared to that in non-fermented preparation-treated ones. We concluded that fermented preparations exerted more potent effects on platelet function both in vitro and ex vivo, possibly as a result of the increased bioavailability of active compounds produced during fermentation. We therefore suggest that fermented products may be potent therapeutics against platelet-related CVDs and can be used as antiplatelet and antithrombotic agents.

• 한국약용작물학회지
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• 제17권5호
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• pp.311-320
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• 2009

The low quality fresh ginseng was fermented by Phelinus linteus or Hericium erinaceum mycelium. This fermented ginseng was extracted by water at $100^{\circ}C$ or water with ultrasonification at $60^{\circ}C$. Total phenolic compounds was improved by ultrasonification extraction process, compare to conventional water extraction. All extracts enhanced the growth of human B and T cells, showing 2.68 times and 3.43 times higher, respectively, than the control. The secretion of TNF-$\alpha$ and IL-6 from human immune cells was enhanced as $3.53{\times}10^{-4}\;pg/cell$, $3.40{\times}10^{-4}\;pg/cell$ by adding H. erinaceum mycelium fermented ginseng. H. erinaceum mycelium fermented ginseng yielded higher nitric oxide production from macrophage than Lipopolysaccharides (LPS). The cytotoxicity on human normal kidney cell (HEK293) was as low as 20.5% in adding the maximum concentration of $1.0\;mg/m{\ell}$ of fermented ginseng. Generally, the extracts from ultrasonification extraction process showed 10% lower toxicity than that by conventional process. H. erinaceum mycelium fermented ginseng had the highest anticancer activity on human lung cancer and stomach cancer cells as 69.33% and 75.32%, respectively at $1.0\;mg/m{\ell}$. It can be concluded that, in general, H. erinaceum mycelium fermented ginseng has relatively better immune and anticancer activities than P. linteus fermented ginseng. Expecially, the extracts treated with ultrasonification had higher activities than that from conventional extraction process.

• 동의생리병리학회지
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• 제21권2호
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• pp.387-391
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• 2007

Recently, much attention has been paid to developing various kinds of fermented herbal extracts, a new type of traditional herbal medicine, in the field of Korean traditional medicine. The fermentation of medicinal herbs is intended to exert a favorable influence on digestability, bioavailavility and pharmacological activity of herbal extract in the gastrointestinal tract. It also produces a number of fermentation products that intensify the nutritional and pharmacological aspects of the medicinal herbs. In order to develop a functional beverage of alleviating the aftereffects of the previous drinks, the extracts (HP-1) of fermented herbal mixture, including Artemisia capillaris Thunb., Lonicera japonica Thunberg, and Hovenia dulcis Thunb., were prepared and the medicinal effect as a hangover cure was evaluated in ethanol-loaded rats. The enzyme activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase-2 (ALDH2) were analyzed by measuring the concentration of blood ethanol and acetaldehyde. The mRNA expression of ADH and ALDH2 was also investigated through RT-PCR analysis. In the HP-1-treated group, the concentration of blood ethanol was significantly reduced at one hour after loading of ethanol, as compared to that in the saline-treated group. The reduced ethanol was converted to acetaldehyde, which resulted in rapid increase in acetaldehyde concentration in an hour. Acetaldehyde was started to decrease at 5 hours after ethanol loading. It implies that HP-1 is highly effective to stimulate the activities of ADH and ALDH2. The HP-1 treatment also activated the mRNA expression of ADH and ALDH. This study suggests that fermented herbal extract, HP-1 can be used as a functional beverage of alleviating the alcohol-induced hangover symptoms by stimulating the activities and gene expression of hepatic alcohol metabolizing enzymes.