Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Comparision of Immuno Activities of Fresh Ginseng Cultured Phelinus Linteus and Hericium erinaceum Mycelium Associated with Ultrasonification Extraction.

저가 수삼을 이용한 상황과 노루궁뎅이 균사체 배양물의 면역 활성 비교

  • Ha, Ji-Hye (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Jeong, Hyang-Suk (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Oh, Sung-Ho (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Jeong, Seung-Seop (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Jeong, Myoung-Hoon (College of Bioscience and Biotechnology, Kangwon National University) ;
  • Jeong, Heon-Sang (College of Agriculture, Life & Environment Sience, Chungbuk National University) ;
  • Jung, Jae-Hyun (Department of Food and Biotechnology, Chungju National University) ;
  • Yu, Kwang-Wan (Department of Food and Biotechnology, Chungju National University) ;
  • Lee, Hyeon-Yong (College of Bioscience and Biotechnology, Kangwon National University)
  • 하지혜 (강원대학교 BT특성화학부대학) ;
  • 정향숙 (강원대학교 BT특성화학부대학) ;
  • 오성호 (강원대학교 BT특성화학부대학) ;
  • 김승섭 (강원대학교 BT특성화학부대학) ;
  • 정명훈 (강원대학교 BT특성화학부대학) ;
  • 정헌상 (충북대학교 식품공학과) ;
  • 정재헌 (충주대학교 식품생명공학과) ;
  • 유광원 (충주대학교 식품생명공학과) ;
  • 이현용 (강원대학교 BT특성화학부대학)
  • Published : 2009.10.30
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Abstract

The low quality fresh ginseng was fermented by Phelinus linteus or Hericium erinaceum mycelium. This fermented ginseng was extracted by water at $100^{\circ}C$ or water with ultrasonification at $60^{\circ}C$. Total phenolic compounds was improved by ultrasonification extraction process, compare to conventional water extraction. All extracts enhanced the growth of human B and T cells, showing 2.68 times and 3.43 times higher, respectively, than the control. The secretion of TNF-$\alpha$ and IL-6 from human immune cells was enhanced as $3.53{\times}10^{-4}\;pg/cell$, $3.40{\times}10^{-4}\;pg/cell$ by adding H. erinaceum mycelium fermented ginseng. H. erinaceum mycelium fermented ginseng yielded higher nitric oxide production from macrophage than Lipopolysaccharides (LPS). The cytotoxicity on human normal kidney cell (HEK293) was as low as 20.5% in adding the maximum concentration of $1.0\;mg/m{\ell}$ of fermented ginseng. Generally, the extracts from ultrasonification extraction process showed 10% lower toxicity than that by conventional process. H. erinaceum mycelium fermented ginseng had the highest anticancer activity on human lung cancer and stomach cancer cells as 69.33% and 75.32%, respectively at $1.0\;mg/m{\ell}$. It can be concluded that, in general, H. erinaceum mycelium fermented ginseng has relatively better immune and anticancer activities than P. linteus fermented ginseng. Expecially, the extracts treated with ultrasonification had higher activities than that from conventional extraction process.

Keywords

References

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