Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Bioconversion Constituents of Galgeun-tang Fermented by Lactobacillus plantarum

Lactobacillus plantarum 발효에 의한 갈근탕의 생물 전환 성분 연구

  • Yang, Min-Cheol (Herbal Medicine Improvement Research Center, Korea Institute of Oriental Medicine) ;
  • Kim, Dong-Seon (Herbal Medicine Improvement Research Center, Korea Institute of Oriental Medicine) ;
  • Jeong, Sang-Won (Herbal Medicine Improvement Research Center, Korea Institute of Oriental Medicine) ;
  • Ma, Jin-Yeul (Herbal Medicine Improvement Research Center, Korea Institute of Oriental Medicine)
  • 양민철 (한국한의학연구원 신한방제제연구센터) ;
  • 김동선 (한국한의학연구원 신한방제제연구센터) ;
  • 정상원 (한국한의학연구원 신한방제제연구센터) ;
  • 마진열 (한국한의학연구원 신한방제제연구센터)
  • Received : 2011.10.13
  • Accepted : 2011.12.02
  • Published : 2011.12.30
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Abstract

Galgeun-tang (GGT) is a traditional medicinal formula that is widely prescribed to treat cold, asthma, and hives in Korea. Fermented herbal medicines can be made more effective than normal herbal medicines by increasing the absorption and bioavailability of the active compounds. In this study, we fermented Galgeun-tang to produce bioconversion constituents using Lactobacillus plantarum (GGT144), and found that four peaks were decreased, three peaks were increased and two new peaks appeared in the HPLC-DAD chromatogram. After HPLC-DAD-guided fractionation of the newly-appearing compounds (1 and 5) and the increased (6, 7, and 9) compounds, the structure of the compounds was determined using NMR and MS. Using this approach the compounds were identified to be pyrogallol (1), daidzein (5), liquiritigenin (6), cinnamyl alcohol (7), and formononetin (9), respectively. In addition, the decreased compounds were identified to be daidzin (2), liquiritin (3), ononin (4), and cinnam aldehyde (8) using HPLC-DAD analysis with standard compounds. The high performance liquid chromatography method was used to quantify the nine constituents in GGT and GGT144. All calibration curves of the standard compounds displayed excellent linearity with a $R^2$ > 0.9968.

Keywords

References

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