• Korean journal of applied entomology
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• v.41 no.2
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• pp.145-150
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• 2002

An exotic Chrysomelid species, Ophraella communa Lesage, which is originally distributed in North America and recently discovered in Korea, is reviewed with its morphological characteristics from egg to adult, brief biological notes, local distribution, and dispersal prospect in Korea.

• Reproductive and Developmental Biology
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• v.35 no.4
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• pp.491-498
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• 2011

During mammalian fertilization, germ cell-specific hyaluronidases, such as sperm adhesion molecule 1 (SPAM1) and hyaluronoglucosaminidase 5 (Hyal5), are important for the dispersal of the cumulus mass. In this study, we demonstrated that bull Hyal5 is a single copy gene on chromosome 4 that is expressed specifically in the testis. In addition, we expressed recombinant bull SPAM1 and Hyal5 in human embryonic kidney 293T cells and showed that these enzymes possessed hyaluronidase activity. We also demonstrated that a polyclonal antibody against bull sperm hyaluronidase inhibits sperm-egg interactions in an in vitro fertilization (IVF) assay. Our results suggested that bull Hyal5 may have a critical role in bull fertilization.

• Korean journal of applied entomology
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• v.5_6
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• pp.59-63
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• 1968

This Paper deals With the biology and the life Cycle of Eriophyes kuko KISHIDA. 1. The host of Eriophyes kuko KISHIDA is Lycium chinense MILL (Boxthorn), Solanum nigrum LINNE(Morel) and Capacium annum LINNE(Red pepper) etc. 2. Eriophyes kuko KISHIDA is migrated and propagated by physical carriers. The direction of the wind has a great influence upon migration and dispersal. 3. The number of e99s deposited by a adult female is $28.5\pm0.449$ egg period is $7.0\pm0.447$ and larval period is $4.33\pm0.667$ days respectively. 4. The size of gall diameter on the leaf of Lycium Chinense MILL after boring in each date by Eriophyes kuke KISHIDA is as follows, 1st day after boring is $290.5\pm0.3415\mu$ End 25st day after boring is $3260.5\pm0.5157\mu$ in average. 5. Adult periods are from 25 to 30 days and the egg laying period is 6 days. 6. Eriophyes kuko KISHIDA has 6 to 7 generations in a year, and hibernate they over winter in the gall as an adult. The first adult appears in late May.

• Journal of Animal Science and Technology
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• v.65 no.6
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• pp.1205-1213
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• 2023

The goals of the present study were to develop a simple method for obtain highly purified pig sperm hyaluronidase (pHyase) and to assess its activity, function, and safety. In mammals, sperm-specific glycophosphatidylinositol (GPI)-anchored Hyase assists sperm penetration through the cumulus mass surrounding the egg and aids in the dispersal of the cumulus-oocyte complex. Recently, Purified bovine sperm hyaluronidase (bHyase) has been shown to enhance therapeutic drug transport by breaking down the hyaluronan barrier to the lymphatic and capillary vessels, thereby facilitating tissue absorption. Commercially available Hyase is typically isolated from bovine or ovine; which have several disadvantages, including the risk of bovine spongiform encephalopathy, low homology with human Hyase, and the requirement for relatively complex isolation procedures. This study successfully isolated highly purified pHyase in only two steps, using ammonium sulfate precipitation and fast protein liquid chromatography. The isolated Hyase had activity equal to that of commercial bHyase, facilitated in vitro fertilization, and effectively dissolved high molecule hyaluronic acid. This simple, effective isolation method could improve the availability of pHyase for research and clinical applications.

• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.407-414
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• 2021

This study investigated the distribution and overwintering of Phanuromyia ricaniae(Hemiptera: Platygastroidea), an egg parasitoid of Ricania sublimata(Hemiptera: Ricaniidea), in order to acquire basic information for biological control. P. ricaniae is distributed in some areas of Gyeonggi, Chungcheong, Jeolla, and Gyeongnam provinces. P. ricaniae is highly prevalent in the southwestern plains, with a tendency to expand to the northeastern area of Korea in line with the dispersal of R. sublimata. The parasitism of P. ricaniae was 1-8% in the field, and the highest in Suncheon, followed by Gurye and Gongju. P. ricaniae overwintered inside the eggs of R. sublimata from September to June of the next year, and transformed into adults in June. The rapid cold-hardiness of P. ricaniae was the highest in the egg state.

• Korean Journal of Ichthyology
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• v.31 no.4
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• pp.187-194
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• 2019

This study was conducted to know the genetic diversity and population structure of Japanese halfbeak (Hyporhamphus sajori) in the Northeast Asia, using mitochondrial DNA control region. In the present study, a total of 70 individuals were collected from three locations of China (Liaoning), Korea (Tongyeong) and Japan (Wakasa Bay), and 47 individuals sequences from three locations of Japan (Wakasa Bay, Toyama Bay and Mikawa Bay) were downloaded from genbank. A total of 7 haplotypes were identified with 7 polymorphic sites from 358 bp length sequences. Haplotype and nucleotide diversity were very low and ranged from 0 to 0.295±0.156 and 0 to 0.0009±0.0011, respectively. Ancestral haplotype was shared by 94% individuals. An extremely low haplotype and nucleotide diversity, and starlike minimum spanning tree indicated that the species have undergone a recent population expansion after bottleneck. Pairwise F_ST values were low and there was no significant differences among populations suggesting a gene flow among the populations. Dispersal of the eggs with the aid of drifting seaweed and currents might be the major responsible factor for the genetic homogeneity.