Browse > Article

Identification and Characterization of Bull Sperm Hyaluronidase Hyal5  

Kim, Ekyune (College of Pharmacy, Catholic University of Daegu)
Chang, Kyu-Tae (National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Reproductive and Developmental Biology / v.35, no.4, 2011 , pp. 491-498 More about this Journal
Abstract
During mammalian fertilization, germ cell-specific hyaluronidases, such as sperm adhesion molecule 1 (SPAM1) and hyaluronoglucosaminidase 5 (Hyal5), are important for the dispersal of the cumulus mass. In this study, we demonstrated that bull Hyal5 is a single copy gene on chromosome 4 that is expressed specifically in the testis. In addition, we expressed recombinant bull SPAM1 and Hyal5 in human embryonic kidney 293T cells and showed that these enzymes possessed hyaluronidase activity. We also demonstrated that a polyclonal antibody against bull sperm hyaluronidase inhibits sperm-egg interactions in an in vitro fertilization (IVF) assay. Our results suggested that bull Hyal5 may have a critical role in bull fertilization.
Keywords
Fertilization; Hyaluronidase; Cumulus cell mass; Zona-pellucida;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Primakoff P, Myles DG (2002): Penetration, adhesion, and fusion in mammalian sperm-egg interaction. Science 296:2183-2185.   DOI
2 Reitinger S, Laschober GT, Fehrer C, Greiderer B, Lepperdinger G (2007): Mouse testicular hyaluronidase- like proteins SPAM1 and HYAL5 but not HYALP1 degrade hyaluronan. Biochem J 401:79-85.   DOI
3 Tung KS, Primakoff P, Woolman-Gamer L, Myles DG (1997): Mechanism of infertility in male guinea pigs immunized with sprem PH-20. Biol Reprod 56:1133- 1141.   DOI   ScienceOn
4 Li MW, Yudin AI, VandeVoort CA, Sabeur K, Primakoff P, Overstreet JW (1997): Inhibition of monkey sprerm hyalurinidase activity and heterologous cumulus penetration by flavonoids. Biol Reprod 56: 1383-1389.   DOI   ScienceOn
5 Yudin AI, Cherr GN, Vandevoort CA, Overstreet JW (1998): Rearrangement of the PH-20 protein on the surface of macaque spermatozoa following exposure to anti-PH-20 antibodies or binding to zona pellucida. Mol Reprod Dev 50:207-220.   DOI   ScienceOn
6 Kim E, Lee JW, Baek DC, Lee SR, Kim MS, Kim SH, Imakawa K, Chang KT (2008): Identification of novel retromer complexes in the mouse testis. Biochem Biophys Res Commun 375:16-21.   DOI   ScienceOn
7 Kimura M, Kim E, Kang W, Yamashita M, Saigo M, Yamazaki T, Nakanishi T, Kashiwabara S, Baba T (2009): Functional roles of mouse sperm hyaluronidases, HYAL5 and SPAM1, in fertilization. Biol Reprod 81:939-947.   DOI   ScienceOn
8 Lin Y, Kimmel LH, Myles DG, Primakoff P (1993):Molecular cloning of the human and monkey sperm surface protein PH-20. Proc Natl Acad Sci USA 90:10071-10075.   DOI   ScienceOn
9 Lin Y, Mahan K, Lathrop WF, Myles DG, Primakoff P (1994): A hyaluronidase activity of the sperm plasma membrane protein PH-20 enables sperm to penetrate the cumulus cell layer surrounding the egg. J Cell Biol 125:1157-1163.   DOI   ScienceOn
10 Myles DG, Primakoff P (1997). Why did the sperm cross the cumulus? To get to the oocyte. Functions of the sperm surface proteins PH-20 and fertilin in arriving at, and fusing with, the egg. Biol Reprod 56:320-327.   DOI   ScienceOn
11 Nishimura H, Kim E, Fujimori T, Kashiwabara S, Kuroiwa A, Matsuda Y, Baba T (2002): The ADAM1a and ADAM1b genes, instead of the ADAM1 (fertilin alpha) gene, are localized on mouse chromosome 5. Gene 291:67-76.   DOI
12 Overstreet JW, Lin Y, Yudin AI, Meyers SA, Primakoff P, Myles DG, Katz DF, Vandevoort CA (1995): Location of the PH-20 protein on acrosome-intact and acrosome-reacted spermatozoa of cynomolgus macaques. Biol Reprod 52:105-114.   DOI   ScienceOn
13 Parrish JJ, Susko-Parrish JL, Leibfried-Rutledge ML, Critser ES, Eyestone WH, First NL (1986): Bovine in vitro fertilization with frozen-thawed semen. Theriogenology 25:591-600.   DOI   ScienceOn
14 Phelps BM, Primakoff P, Koppel DE, Low MG, Myles DG (1988): Restricted lateral diffusion of PH-20, a PI-anchored sperm membrane protein. Science 240: 1780-1782.   DOI
15 Baba D, Kashiwabara S, Honda A, Yamagata K, Wu Q, Ikawa M, Okabe M, Baba T (2002): Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg. J Biol Chem 277:30310-30314.   DOI
16 Bradford MM (1976): A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.   DOI   ScienceOn
17 Cherr GN, Yudin AI, Overstreet JW (2001): The dual functions of GPI-anchored PH-20: hyaluronidase and intracellular signaling. Matrix Biol 20:515-525.   DOI   ScienceOn
18 Gmachl M, Kreil G (1993): Bee venom hyaluronidase is homologous to a membrane protein of mammalian sperm. Proc Natl Acad Sci USA 90:3569- 3573.   DOI   ScienceOn
19 Huh JW, Kim YH, Kim DS, Park SJ, Lee SR, Kim SH, Kim E, Kim SU, Kim MS, Kim HS (2010): Alu-derived old world monkeys exonization event and experimental validation of the LEPR gene. Mol Cells 30:201-207.   DOI   ScienceOn
20 Hooft van Huijsduijnen R (1998): ADAM 20 and 21; two novel human testis-specific membrane metalloproteases with similarity to fertilin-alpha. Gene 206:273-282.   DOI   ScienceOn
21 Kang W, Zhou C, Koga Y, Baba T (2010): Hyaluronan- degrading activity of mouse sperm hyaluronidase is not required for fertilization? J Reprod Dev 56:140-144.   DOI   ScienceOn
22 Kim E, Baba D, Kimura M, Yamashita M, Kashiwabara S, Baba T (2005): Identification of a hyaluronidase, Hyal5, involved in penetration of mouse sperm through cumulus mass. Proc Natl Acad Sci U S A 102:18028-18033.   DOI   ScienceOn