• 한국환경복원기술학회지
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• 제15권2호
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• pp.117-125
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• 2012

The objective of this study was to investigate the possibility of planting seedlings for quarry rehabilitation. To achieve the objective, the experiment was designed for rehabilitation of quarry with planting seedlings and seeding types. Planting seedlings were categorized as target species, accompanied species and pioneer species. The study was conducted in limestone quarry (Lafarge Halla Cement Inc.) near the Baekdudaegan Mountains at Okke, Kangwon-do. The experimental planting bed was set in 2007 and field monitoring was carried out from 2007 to 2011. As the result of experiment, it was found that the early-phase pattern for surveyed species to establish was affected by the planting and seeding types. As years after planting and seeding, the percent of plant coverage also increased up to 90%. The methods of mixed planting and seeding were good for species diversity, but the growing of seedlings were affected by seeding plants. Accompanied species and pioneer species were superior to target species during first 2 years, but target species has gained predominance during last 2 years. The quality maintenance should be carried out annually to attain the goal of rehabilitation.

• 미생물학회지
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• 제36권4호
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• pp.249-253
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• 2000

Cyanobacteria의 광합성 보조색소인 phycocyanin의 PC operon(cpc gene)을 PCR로 증폭하고, 제한효소로 처리하여 RFLP pattern을 비교하였다. Intergenic spacer sequence를 포함한 cpc gene은 실험에 사용한 cyanobacteria 균주 모두에게 증폭되었으며, 산물의 size는 약 700 bp였다. PCR산물을 5종의 제한효소로 처리한 결과 AluI, MspI, HaeIII는 같은 속내으ㅐ 균주간에 동일한 pattern을 나타내어 속 구분이 가능하였으며 CfoI은 Anabeana와 Synechocystis속의 균주간에 구별되는 양상을 나타내어 속내 균주 구별에 유용하였다. Restriction enzyme profile에 의한 phenogram에서 Anabeana, Chlorogloea, Synechyhocystis는 각각 하나의 cluster를 형성하여 cyanobacteria의 분류에 PC-IGS의 RFLP pattern이 유용함을 알 수 있었다.

• Journal of Acupuncture Research
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• 제19권1호
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• pp.225-235
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• 2002

Objective : This study was carried out to review Chang-Won Lee's theory of Sasangand acupuncture of constitution. Methods : We have investigated Chang-Won Lee's unknown literature "Medicine of sassang "(四象醫學). Results : Constitutional acupuncture is based on the diversity of human being and medically developed the response variation to disease and acupuncture. The diversity is due to the Organ's Congenital Formation Variation based on human nature and it is categorized as four from physiology, pathology, symptoms, to therapy. Chang-Won Lee's constitutional acupuncture discriminated constitution of comparison palpations of right and left both sides. He insisted that constitution only four type(Sasang), not exist man of To-Hyoung (土形人) in "young -chu"(靈樞) and his constitutional acupuncture all used Jung-kyuck (reinforcement acupuncture method) and Seong-kyuck(reducing acupuncture method) of Sa-am(舍岩)

• 한국조경학회지
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• 제21권4호
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• pp.83-99
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• 1994

To compare basic information and to inspect impacts situations between 1987 and 1992, this study was executed in Chongnung valley of Mt. Puk'an. And to provide basic informations through the change of plant species diversity, some methods were applied and analyzed such as the degree of environmental impacts and belt-transect method. The results were summarized as follows; 1. In order to find environmental impacts by visitor, the degree of environmental impacts class that was proposed by Kwon(1991) was implicated. In 4 sites, total area of over impact class 2 is 86,692$m^2$ and area of impacts class 4-6 that is impossible to recover naturally is 36,856$m^2$. Totally, the impacted area was profounded qualitatively and quantitatively against 1987's. 2. In the vegetation change by visitor's impacts, species diversity was rapidly decreased as nearer the campsite and trail. And in the basal area and coverage, lower layer species was sensitively changed. Trampling-resistent species is as following; Robinia pseudoacacia, Lespedeza cyrtobotrya, Staphylea bumalda, Clerdendron trichotomum, Stephanandra incisa, Rubus crataegifolius, Lespedeza maximowiczii, Weigela subsessilis, Rosa multiflora, Zanthoxylum schinifolium, Lindera obtusiloba, Callicarpa japonica, Sorbus alnifolia, Symplocos mongolica and so on. In the results of soil hardness test, that was decreased as far away from campsite and trail. 3. In the 4 sites selected in Chongnung valley of Puk'ansan Natioal Park, management plans was largely divided 4 category, such as trail improvement, facilities setting, ecotone vegetation establishment, and vegetation rehabilitation. And a suggestion was provided to each site.

• Journal of Information Processing Systems
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• 제13권4호
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• pp.1000-1013
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• 2017

Clustering is a NP-hard problem that is used to find the relationship between patterns in a given set of patterns. It is an unsupervised technique that is applied to obtain the optimal cluster centers, especially in partitioned based clustering algorithms. On the other hand, cat swarm optimization (CSO) is a new meta-heuristic algorithm that has been applied to solve various optimization problems and it provides better results in comparison to other similar types of algorithms. However, this algorithm suffers from diversity and local optima problems. To overcome these problems, we are proposing an improved version of the CSO algorithm by using opposition-based learning and the Cauchy mutation operator. We applied the opposition-based learning method to enhance the diversity of the CSO algorithm and we used the Cauchy mutation operator to prevent the CSO algorithm from trapping in local optima. The performance of our proposed algorithm was tested with several artificial and real datasets and compared with existing methods like K-means, particle swarm optimization, and CSO. The experimental results show the applicability of our proposed method.

• 생태와환경
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• 제54권3호
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• pp.221-228
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• 2021

Recently, environmental DNA (eDNA)-based metabarcoding approaches have been proposed to evaluate the status of freshwater ecosystems owing to various advantages, including fast and easy sampling and minimal habitat disruption from sampling. Therefore, as a case study, we applied eDNA metabarcoding techniques to evaluate the effects of an abandoned mine land located near a headwater stream of Nakdonggang River, South Korea, by examining benthic macroinvertebrate diversity and compared the results with those obtained using the traditional Surber-net sampling method. The number of genera was higher in Surber-net sampling (29) than in the eDNA analysis (20). The genus richness tended to decrease from headwater to downstream in eDNA analysis, whereas richness tended to decrease at sites with acid-sulfated sediment areas using Surber-net sampling. Through cluster analysis and non-metric multidimensional scaling, the sampling sites were differentiated into two parts: acid-sulfated and other sites using Surber-net sampling, whereas they were grouped into the two lowest downstream and other sites using eDNA sampling. To evaluate freshwater ecosystems using eDNA analysis in practical applications, it is necessary to constantly upgrade the methodologies and compare the data with field survey methods.

• International Journal of Industrial Entomology and Biomaterials
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• 제41권2호
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• pp.56-62
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• 2020

Mulberry (Morus spp. family: Moraceae) has prime importance in the sericulture industry, and its foliage is the only natural feed of the silkworm Bombyx mori L. Traditional classification methods using morphological traits were largely unsuccessful in assessing the diversity and relationships among different mulberry species because of environmental influences on the traits of interest. For these reasons, it is difficult to differentiate between the varieties and cultivars of Morus spp. In the present study, inter-simple sequence repeat (ISSR) markers were used to investigate the genetic diversity of 48 mulberry samples genotyped using nine ISSR primers. The ISSR markers exhibited polymorphisms (53.2%) among mulberry genotypes. Furthermore, similarity coefficient estimated for these ISSR markers was found to vary between 0.67 and 0.99 for the combined pooled data. The phenogram drawn using the UPGMA cluster method based on combined pooled data of the ISSR markers divided the 48 mulberry genotypes into seven major groups. No genetic association was found in the collection area, and there was a mixed pattern between the mulberry lines. The hybridization between different mulberry species is highly likely to be homogenized due to natural hybridization.

• Animal Bioscience
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• 제35권7호
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• pp.949-954
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• 2022

Objective: The present study is aimed at phenotypic characterization and mitochondrial d-loop analysis of indigenous "Diara" buffalo population, which are mostly confined to the villages on the South and North Gangetic marshy plains in the Bihar state of India. These buffaloes are well adapted and are best suited for ploughing and puddling the wet fields meant for paddy cultivation. Methods: Biometric data on 172 buffaloes were collected using a standard flexible tape measure. Animals are medium in size; the typical morphometric features are long head with a broad forehead and moderately long and erect ears. Genomic DNA was isolated from unrelated animals. The mtDNA d-loop 358-bp sequence data was generated and compared with 338 sequences belonging to riverine and swamp buffaloes. Results: Based on the mitochondrial d-loop analysis the Diara buffaloes were grouped along with the haplotypes reported for riverine buffalo. Sequence analysis revealed the presence of 7 mitochondrial D loop haplotypes with haplotype diversity of 0.9643. Five of the haplotypes were shared with established swamp breeds and with Buffalo population of Orissa in India. Conclusion: Morphometric analyses clearly shows distinguishing features like long and broad forehead which may be useful in identification. The germplasm of Diara buffalo is much adapted to the marshy banks of river Ganga and its tributaries. It constitutes a valuable genetic resource which needs to be conserved on priority basis.

• 한국멀티미디어학회논문지
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• 제24권11호
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• pp.1567-1579
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• 2021

This study describes a multicultural literacy program that can nurture multicultural people who can live in a multicultural society. A total of 10 20-hour programs were conducted for 22 youths in Busan. Through this, the current perception and acceptance of multiculturalism among young people in Busan was investigated, and a curriculum suitable for multicultural awareness, multicultural acceptance, and multicultural competency enhancement was constructed. In accordance with the goal of strengthening the multicultural competency required for multicultural activists through education, learners can exchange opinions through the activity sheet, so that learners can know the difference between themselves and others. Students could learn about each other's culture and values through cultural exchange with others, and through teachers with diverse cultures, they could learn various perspectives and cultures about culture and values. Through the results of the multicultural literacy program, I would like to suggest three methods of multicultural literacy education to strengthen multicultural competence in a multicultural society. Therefore, efforts should be made to develop various multicultural education courses to strengthen the multicultural capabilities of Koreans.

• Journal of Ginseng Research
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• 제46권1호
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• pp.91-103
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• 2022

Background: Red ginseng extract boosts immunity against inflammation and cancer in the human body. However, studies on the effects of red ginseng extract on the gut microbiome remain unexplored. Methods: In 2019, the positive effects and changes in the gut microbiome after administering 1 pack (3 g) of red ginseng extract per day to 53 adults aged 40 to 75 for 24 weeks were investigated. The gut microbial environment changes were qualitatively and quantitatively analyzed using next-generation sequencing and real-time polymerase chain reaction technology. Results: On comparing and analyzing alpha diversity and beta diversity, the microbial pattern showed significant differences (OTUs p = 0.003, chao1 p < 0.001, Bray-Curtis p = 0.001) before and after ingestion of red ginseng extract, indicating that gut microbial richness increased after ingestion. Moreover, after comparing and analyzing the gut microbiome's differences after red ginseng extract intake, significant differences were noted between three strains at the phylum level and among 57 strains at the genus level. Conclusion: This study proposes the potential use of red ginseng extract as a prebiotic after confirming its positive effects, including increasing gut microbiome richness, reducing harm to the gut microbiome, and increasing the number of some strains in the gut microbiome.