International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Analysis of the Genetic Relationship among Mulberry (Morus spp.) Cultivars Using Inter-Simple Sequence Repeat (ISSR) Markers

  • Park, Eun-Ju (Department of Analysis & Certification, FACT) ;
  • Kang, Min-Uk (Department of Analysis & Certification, FACT) ;
  • Choi, Myoung-Seob (Department of Analysis & Certification, FACT) ;
  • Sung, Gyoo-Byung (Department of Agricultural Biology, NAS, RDA) ;
  • Nho, Si-Kab (College of Agricultural and Life Science, Kyungpook National University)
  • Received : 2020.12.07
  • Accepted : 2020.12.18
  • Published : 2020.12.31
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Abstract

Mulberry (Morus spp. family: Moraceae) has prime importance in the sericulture industry, and its foliage is the only natural feed of the silkworm Bombyx mori L. Traditional classification methods using morphological traits were largely unsuccessful in assessing the diversity and relationships among different mulberry species because of environmental influences on the traits of interest. For these reasons, it is difficult to differentiate between the varieties and cultivars of Morus spp. In the present study, inter-simple sequence repeat (ISSR) markers were used to investigate the genetic diversity of 48 mulberry samples genotyped using nine ISSR primers. The ISSR markers exhibited polymorphisms (53.2%) among mulberry genotypes. Furthermore, similarity coefficient estimated for these ISSR markers was found to vary between 0.67 and 0.99 for the combined pooled data. The phenogram drawn using the UPGMA cluster method based on combined pooled data of the ISSR markers divided the 48 mulberry genotypes into seven major groups. No genetic association was found in the collection area, and there was a mixed pattern between the mulberry lines. The hybridization between different mulberry species is highly likely to be homogenized due to natural hybridization.

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References

  1. Agarwal M, Shrivastava N, Padh H (2008) Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Rep 27, 617-631. https://doi.org/10.1007/s00299-008-0507-z
  2. Awasthi AK, Nagaraja GM, Naik GV, Kanginakudru S, Thangavelu K, Nagaraju J (2004) Genetic diversity and relationships in mulberry (genus Morus) as revealed by RAPD and ISSR marker assays. BMC Genet 5(1), 1.
  3. Camacho FJ, Liston A (2001) Population structure and genetic diversity of Botrychium pumicola (Ophioglossaceae) based on inter-simple sequence repeats (ISSR). Am J Bot 88, 1065-1070. https://doi.org/10.2307/2657089
  4. Grover A, Sharma PC (2016) Development and use of molecular markers: Past and present. Crit Rev Biotechnol 36(2), 290-302. https://doi.org/10.3109/07388551.2014.959891
  5. Gupta S, Srivastava M, Mishra GP, Naik PK, Chauhan RS, Tiwari SK, et al. (2008) Analogy of ISSR and RAPD markers for comparative analysis of genetic diversity among different Jatropha curcas genotypes. Afr J Biotechnol 7, 4230-4243.
  6. Iruela M, Rubio J, Cubero JI, Gil J, Millan T (2002) Phylogenetic analysis in the genus Cicer and cultivated chickpea using RAPD and ISSR markers. Theor Appl Genet 104, 643-651. https://doi.org/10.1007/s001220100751
  7. Kalpana D, Choi SH, Choi TK, Senthil K, Lee YS (2012) Assessment of genetic diversity among varieties of mulberry using RAPD and ISSR fingerprinting. Sci Hortic 134, 79-87. https://doi.org/10.1016/j.scienta.2011.11.002
  8. Kim HB, Seo SD, KOO HY, Seo YS, Kim SL, Sung GB (2013) Quantitative Analysis of 1-deoxynojirimycin in Mulberry Leaves, J Seric Entomol Sci 51(1), 20-29. https://doi.org/10.7852/JSES.2013.51.1.20
  9. Kondo Y (1957) Trace constituents of mulberry leaves. Nippon Sanshigaku Zasshi 26, 349-352.
  10. Lee WC, Kim AJ, Kim SY (2003) The study on the functional materials and effects of mulberry leaf. Food Sci Ind 36(3), 2-14.
  11. Machii H, Koyama A, Yamanouchi H (1999) A list of genetic mulberry resources maintained at National Institute of Sericultural and Entomological Science. Misc Publ Natl Seric Entomol Sci (Japan) 26, 1-77.
  12. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8, 4321-4325. https://doi.org/10.1093/nar/8.19.4321
  13. Nagaoka T, OgiharaY (1997) Applicability of inter-simple sequence repeat polymorphisms in wheat for use as DNA markers in comparison to RFLP and RAPD markers. Theor Appl Genet 94, 597-602. https://doi.org/10.1007/s001220050456
  14. Ryu HJ, Bae CH (2011) Genetic diversity and relationship analysis of Taraxacum officinale Weber and Taraxacum coreanum Nakai accessions based on Inter-Simple Sequence Repeats (ISSR) markers. Korean J Med Crop Sci 19, 149-156. https://doi.org/10.7783/KJMCS.2011.19.3.149
  15. Schlotterer C (2004) The evolution of molecular markers-Just a matter of fashion? Nat Rev Genet 5(1), 63-69. https://doi.org/10.1038/nrg1249
  16. Sung GB, Ryu KS, Kim HR, Nam HW, Goo TW, Cho SY (2001) Analysis of ITS nucleotide sequences in ribosomal DNA of Morus species. J Seric Entomol Sci 43, 1-8.
  17. Vijayan K, Kar PK, Tikader A, Srivastava PP, Awasthi AK, Thangavelu K, et al. (2004) Molecular evaluation of genetic variability in wild populations of mulberry (Morus serrata Roxb.). Plant Breed 123, 568-572. https://doi.org/10.1111/j.1439-0523.2004.01035.x
  18. Vijayan K, Tikader A, Kar PK, Srivastava PP, Awasthi AK, Thangavelu K, et al. (2006) Assessment of genetic relationships between wild and cultivated mulberry (Morus) species using PCR based markers. Genet Resour Crop Evol 53, 873-882. https://doi.org/10.1007/s10722-004-6148-3
  19. Williams JGK, Kubelik ARK, Rafalski JA, Tingey V (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18, 6531-6535. https://doi.org/10.1093/nar/18.22.6531
  20. Zietkiewicz E, Rafalski A, Labuda D (1994) Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20, 176-183. https://doi.org/10.1006/geno.1994.1151