• Title/Summary/Keyword: dilution method

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Studies on the Radioimmunoassay of Human Growth Hormone - 1. Evaluation of the method of determination (사람성장(成長)홀몬의 방사면역측정(放射免疫測定)에 관한 연구 - 제I편 사람성장(成長)홀몬의 측정법에 관한 검토)

  • Lee, Young-Woo;Lee, Hong-Kyu;Koh, Chang-Soon;Lee, Mun-Ho
    • The Korean Journal of Nuclear Medicine
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    • v.6 no.1
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    • pp.17-24
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    • 1972
  • Utilizing the commercial radioimmunoassay kit, the author assayed HGH and evaluated the problems of the method. The method had the sensitivity of 0.5 mug/ml degree and could determine the plasma HGH concentration directly without the help of plasma extraction. Also it was specific for the HGH, when tested by a dilution method using test utilizing the plasma of the acromegalic patient. The author also obtained the $^{125}I$ labelled HGH of specific activity of $156.3{\mu}Ci/{\mu}g$, performing the chloramine-T method.

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Antimicrobial Effects of Lonicera japonica against Gram Positive and Gram Negative Anaerobic Bacteria

  • Rhee, Ki-Hyeong;Lee, Keyong-Ho
    • Natural Product Sciences
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    • v.17 no.1
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    • pp.23-25
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    • 2011
  • It has been shown that the butanol extract of Lonicera japonica has antimicrobial and other potentially useful biological activities. The purpose of this study was to determine the in vitro activity of Lonicera japonica compared to other antimicrobial agents against anaerobic bacteria. Specifically, the in vitro activity of the butanol extract was investigated against 104 clinical isolates of anaerobic bacteria using an agar dilution method and the results were compared to erythromycin, cefoxitin, imipenem, clindamycin, and metronidazole. It was found that Lonicera japonica and imipenem were the most active antimicrobial agents tested.

Microcrystalline Silicon for Thin Film Transistor

  • Milovzorov, D.;Kim, K.B.;Lisachenko, M.;Seo, J.W.;Lee, K.Y.;Chung, H.K.
    • 한국정보디스플레이학회:학술대회논문집
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    • 2005.07b
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    • pp.1320-1322
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    • 2005
  • Microcrystalline silicon films were deposited on glass substrate by using plasma-enhanced chemical vapor deposition (PECVD) method. The crystalline volume fraction was estimated by means of Raman spectrometer with argon laser as light source. The high hydrogen dilution of silane gas was used for increase in content of crystal silicon phase.

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Studies on manufacture system of Hwanggum(黃芩) aqua-acupuncture by Boiling water Method (전탕에 의한 약침 황금제제의 제조에 관한 연구)

  • Go, Byeong-Seop;Ju, Hye-Jeong;Ma, Jin-Yeol;Park, Gap-Ju;An, Sang-U
    • Journal of Pharmacopuncture
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    • v.1 no.1
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    • pp.77-86
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    • 1997
  • In order to study the reproducibility and effeciency of the manu-pacture system Hwanggum(黃芩) aqua-acupuncuture was prepared. The filtra-tion and dilution have been mainly employed in the study of the manupacture step. These methods were showed possibility of standardization and variety for aqua-acupuncture.

Relationship silk reelability with nonpermeative cocoon cooking after chemical treatment (화학무삼투백색자견이 사조에 미치는 영향)

  • 최병희;유시환
    • Journal of Sericultural and Entomological Science
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    • v.5
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    • pp.43-46
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    • 1965
  • This report was prepared to check silk reelalility and its quality when the cocoon was cooked in nonpermeative condition after trotted with some chemical solution. The obtained results were as followings. 1. A new chemical, Serisol, was developed by the author to help silk reelability 2. The chemical cooking method showed 0.4% increase of silk yield more than the normal silk reeling method. 3. The chemical conducted good cocoon cooking without permeative process. 4. This method showed better cleanness and neatness than the normal one. 5. This method did not damage the silk quality because of use Serisol. 6. The proper dilution of Serisol was found as 2000 times for it.

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Effect of Maunfacturing Methods on Content Uniformity of Tablets (제제방법(製劑方法)이 정제(錠劑)의 함량균일성(含量均一性)에 미치는 영향(影響))

  • Lee, Min-Hwa;Choi, Hoo-Kyun;Kim, Kil-Soo
    • Journal of Pharmaceutical Investigation
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    • v.11 no.1
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    • pp.6-14
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    • 1981
  • Safety, efficacy and reliability are the three basic criteria that define the quality of any well-designed pharmaceutical dosage form. Content uniformity directly bears on each of the three criteria defining the quality of drug product. Effect of manufacturing methods and physicochemical properties of the drug on content uniformity of tablets was investigated. Chlorpheniramine maleate and micronized salicylic acid were used as main ingredients. Three different methods for incorporation of main ingredients with diluents were solvent mixing method, geometric dilution method, and simple mixing method. The solvent mixing method was the best one of the three.

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Antimutagenic and Antibacterial Activities of Korean and American Propolis (한국산과 미국산 프로폴리스의 항돌연변이 및 항균효과)

  • Jang, Il-Woong;Park, Jeong-Seob;Kwon, Hyoung-Cheol;Jung, Mun-Yhung;Choi, Dong-Seong
    • Korean Journal of Food Science and Technology
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    • v.41 no.6
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    • pp.694-699
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    • 2009
  • The antimutagenic activities of ethanol extracts of Korean and American propolis were tested using Salmonella Typhimurium TA98 with two indirect mutagens of 3-amino-1,4-dimethyl-5H-pyrido [4,3-b]indole (Trp-P-1) and 2-aminoanthracene (2-AA) with S9 mix. Additionally, their antimicrobial activities against acne-related pathogenic strains of Propionibacterium acnes, Staphylococcus Epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa were evaluated using both paper disk method and agar dilution method. Ethanol extracts of Korean and American propolis showed strong inhibitory effects, in a dose dependant manner, against the mutagenicities induced by Trp-P-1 and 2-AA. The antimutagenic effect of ethanol extracts of Korean propolis showed significantly higher protective activity than that of American propolis against the Trp-P-1 induced mutagenicity of S. Typhimurium TA98 at the lower concentration ($1-10\;{\mu}g$), but significantly lower protective activity at the higher concentration ($50-200\;{\mu}g$). The antimutagenic effect of ethanol extract of Korean propolis showed significantly higher protective activity than that of American propolis against the 2-AA induced mutagenicity at the concentration of $1\;{\mu}g$, but significantly lower protective activity than that of the American at the higher concentration ($50-200\;{\mu}g$). Both extracts showed strong antimicrobial activities against all the acne-related pathogens tested, with minimal inhibitory concentration (MIC) values in the range $1,500-5,000\;{\mu}g/mL$.

The Influence of Ammonium-Nitrogen on Anaerobic Microorganisms in Swine Wastewater by Batch-Fermentation. (혐기성 회분식 배양에서 양돈폐수의 NH$_4$-Nitrogen이 혐기성 미생물에 미치는 영향)

  • 김연옥
    • Microbiology and Biotechnology Letters
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    • v.26 no.2
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    • pp.173-178
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    • 1998
  • This study presents the influence of ammonium-nitrogen on microorganisms in swine wastewater. For the anaerobic batch fermentation, two different methods were used. One is the dilution of wastewater with water. The other method is the elimination of ammonium-nitrogen from the wastewater. By addition of MgO into wastewater, non-soluble crystall was formed under alkaline condition as MgNH$_4$PO$_4$6$H_2O$ (MAP). The master culture was adapted in swine wastewater for more than 3 months, in water-dilution method, the dilution of wastewater with 25% water gave us the best result in efficiency of COD removal. Two hundred hours later MAP-treated wastewater showed the efficiency of the COD removal more than 80%. Under same condition obtained none MAP-treated wastewater about 50%. MAP treatment carried out the very effective anaerobic digestion with swine wastewater. The important result in this study is that the low ratio of C:N influenced on anaerobic microorganisms more than high concentration of ammonium nitrogen in swine wastewater. The struvite for the crystallforming has no toxic effect on methanogenic bacteria.

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A Study about Improvement of Multiple Odor of Public Toilet (Dong-gu, Daejeon) (공중화장실 복합악취 및 개선에 관한 연구 (대전광역시 동구))

  • Jo, Soo-Jun;Shin, Je-Youn;Oh, Sang-Soo;Yu, Dae-Yong;Lee, Sung-Jae
    • Journal of Korean Society of Environmental Engineers
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    • v.37 no.4
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    • pp.240-245
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    • 2015
  • This study aimed to provide the basic data to establish measures for the management of the public toilets and reduction of the offensive odor through the air dilution olfactory method and instrumental analysis about offensive odor dilution rate of public toilets, focusing on the Dong-gu, Daejeon Metropolitan City, Korea. The offensive odor intensities ranged from 10 degree to 669 degrees in the urinal toilets and from 31 to 464 in closet bowl toilets. Meanwhile, toilets that had offensive odor of more than 2.5, of which a civil complaint can be filed, were researched 6 (33%). Offensive odor intensities were different according to the closet bowl toilets of the flush, fermented and conventional methods and the year of their installation, but not according to the area of the toilets. As a result of the instrumental analysis targeting 6 public toilets to identify the offensive odor substance, the maximal concentration of $NH_3$ was 2.04 ppm and the average concentration was 0.67 ppm, but $H_2S$, $CH_3SH$ and $(CH_3)_3N$ were not detected. In the fermented and conventional public toilets were not soaps, air fresheners and wastebaskets. Just 50% of the flush toilets had toilet tissue.