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Antimutagenic and Antibacterial Activities of Korean and American Propolis  

Jang, Il-Woong (Korea Health Supplements Institute, Korea Health Supplements Association)
Park, Jeong-Seob (Department of Bioprocess Engineering, Graduate School, Chonbuk National University)
Kwon, Hyoung-Cheol (Department of Radiation Oncology, Medical School, Chonbuk National University)
Jung, Mun-Yhung (Department of Restaurant Industry and Food Preperation, Woosuk University)
Choi, Dong-Seong (Department of Food and Biotechnology, Woosuk University)
Publication Information
Korean Journal of Food Science and Technology / v.41, no.6, 2009 , pp. 694-699 More about this Journal
Abstract
The antimutagenic activities of ethanol extracts of Korean and American propolis were tested using Salmonella Typhimurium TA98 with two indirect mutagens of 3-amino-1,4-dimethyl-5H-pyrido [4,3-b]indole (Trp-P-1) and 2-aminoanthracene (2-AA) with S9 mix. Additionally, their antimicrobial activities against acne-related pathogenic strains of Propionibacterium acnes, Staphylococcus Epidermidis, Staphylococcus aureus and Pseudomonas aeruginosa were evaluated using both paper disk method and agar dilution method. Ethanol extracts of Korean and American propolis showed strong inhibitory effects, in a dose dependant manner, against the mutagenicities induced by Trp-P-1 and 2-AA. The antimutagenic effect of ethanol extracts of Korean propolis showed significantly higher protective activity than that of American propolis against the Trp-P-1 induced mutagenicity of S. Typhimurium TA98 at the lower concentration ($1-10\;{\mu}g$), but significantly lower protective activity at the higher concentration ($50-200\;{\mu}g$). The antimutagenic effect of ethanol extract of Korean propolis showed significantly higher protective activity than that of American propolis against the 2-AA induced mutagenicity at the concentration of $1\;{\mu}g$, but significantly lower protective activity than that of the American at the higher concentration ($50-200\;{\mu}g$). Both extracts showed strong antimicrobial activities against all the acne-related pathogens tested, with minimal inhibitory concentration (MIC) values in the range $1,500-5,000\;{\mu}g/mL$.
Keywords
propolis; antimutagenic; indirect mutagen; antibacterial; acne;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
Times Cited By SCOPUS : 2
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1 Cizmarik J, Lahitova N. Antimutagenicity of propolis. Pharmazie 53: 883-884 (1998)
2 Takino Y, Mochida S. Propolis its chemical constituents and biological activities. Honeybee Sci. 3: 145-1449 (1982)
3 Aga H, Shibuya T, Sugimoto T, Kurimoto M, Nakajima S. Isolation and Identification of antimicrobial compounds in Brazilian propolis. Biosci. Biotech. Bioch. 58: 945-946 (1994)   DOI
4 Lee SW, Hwangbo S, Kim HJ. Antimicrobial activities of Korean propolis. Korean J. Food Sci. Anim. Resour. 22: 66-71 (2002)   과학기술학회마을
5 Park YK, Ikegaki M. Preparation of water and ethanolic extracts of propolis and evaluation of the preparations. Biosci. Biotech. Bioch. 62: 2230-2232 (1998)   DOI   ScienceOn
6 Ong TM, Mukhtar M, Wolf CR, Zeiger E. Different effects of cytochrome P450-inducers on promutagen activation capabilities and enzymatic activities of S-9 from rat liver. J. Environ. Pathol. Tox. Oncol. 4: 55-60 (1980)
7 Jang, GH, Ahn, BY, Kwon, YJ, Choi, DS. Antimutagenic effect of resveratrol on Trp P-1 in Salmonella typhimurium TA98. Korean J. Food Nutr. 14: 329-332 (2001)
8 Kwak HS, Nam CH, Kwon HS. A study on the essential oil for hair health care. Korean Public Health Res. 31: 27-36 (2005)
9 NCCLS. Performance Standards for Antimicrobial Disk Susceptibility Test. 6th ed. Approved standard, NCCLS document M2-A6, National Committee for Clinical Laboratory Standards, Wayne, PA, USA (1997)
10 Wollenweber E, Hausen BM, Greenway W. Phenolic constituents and sensitizing propwrties of propolis, poplar balsam, and balsam of Peru. Bull. Groupe Polyphenols 15: 112-120 (1990)
11 NCCLS. Methods for Dilution Antimicrobial Susceptibility Test for Bacterial that Grow Aerobically. 3th ed. Approved standard, NCCLS document M7-A3, National Committee for Clinical Laboratory Standards, Vilanova, PA, USA (1993)
12 Shigemi T, Tsutomu W, Tanaka N. Studies on the constituents of Brazilian propolis. Chem. Pharm. Bull. 47: 1388-1392 (1999)   DOI
13 Lee HJ, Bae YI, Jeong CH, Shim KH. Biological activities of various solvent extracts from propolis. Korean J. Food Nutr. 34: 1-7 (2005)   과학기술학회마을   DOI   ScienceOn
14 Burdock GA. Review of the biological properties and toxicity of bee propolis (propolis). Food Chem. Toxicol. 36: 347-363 (1998)   DOI   ScienceOn
15 Carri$\acute{e}$re V, de Waziers I, Courtois YA, Leroux JP, Beaune PH. Cytochrome P450 induction and mutagenicity of 2-aminoanthracene(2AA) in rat liver and gut. Mutat. Res. 268: 11-20 (1992)   DOI   ScienceOn
16 Rao CV, Desai D, Kaul B, Amin S, Reddy BS. Effects of caffeic acid esters on carcinogen-induced mutagenicity and human adenocarcinoma cell growth. Chem. -Biol. Interact. 84: 277-290 (1992)   DOI   ScienceOn
17 Hur YK, Kim NR, Yoon WK, Jo SK, Jung UH, Park HR. Properties of Korean propolis on the antibacterial activity and inhibition of antibiotic-resistant bacteria. Korean J. Apic. 22: 71-78(2007)
18 Ames BN, Maron DM. Revised methods for the S. typhimurium mutagenicity test. Mutat. Res. 113: 173-215 (1983)   DOI
19 Jeng SN, Shih MK, Kao CM, Liu TZ, Chen SC. Antimutagenicity of ethanol extracts of bee glue against environmental mutagens. Food Chem. Toxicol. 38: 893-897 (2000)   DOI   ScienceOn
20 Lindenfelser LA. Antimicrobial activity of propolis. Am. Bee J. 107: 90-92 (1967)   과학기술학회마을
21 Ghisalberti EL. Propolis: A review. Bee World 60: 59-84 (1979)
22 Hashimoto Y, Shudo K, Okamoto T. Activation of a mutagen, 3-amino-1-methyl-5H-pyrido[4,3-b]indole. Identification of 3-hydroxyamino-1-methyl-5H-pyrido [4,3-b]indole and its reaction with DNA. Biochem. Bioph. Res. Co. 96: 355-362 (1980)   DOI   ScienceOn
23 Walker P, Crane E. Constituents of propolis. Apodologie 18: 327-334 (1987)   DOI
24 Park HK, Kim SM, Shim CH. Antimicrobial activity of water soluble propolis. Korean J. Food Nutr. 21: 15-21 (2008)   과학기술학회마을
25 Metzner J, Bekemeier H, Paintz M, Schneidewind E. On the antimicrobial activity of propolis and propolis constituents. Pharmazie 34: 97-102 (1979)   ScienceOn
26 Son YR. Studies of the antimicrobial effect of extracts of propolis. J. Food Hyg. Saf. 18: 189-194 (2003)   과학기술학회마을
27 Greenaway W, May J, Scaysbrook T, Whatley FR. Identification by gas chromatography-mass spectrometry of 150 compounds in propolis. Z. fur Naturforsch. 46c: 111-121 (1990)