• 대한한의학회지
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• 제27권1호
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• pp.11-22
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• 2006

Objectives : In this study, the aim was to investigate the inhibitory effects of Bangpungtong-seoung-san on the obese gene and obese inhibitory about obese-mouse induced by high fat diet. Methods : In order to investigate the effects of Bangpuangtongseoung-san(BPTS) on the obese gene and obese inhibitory, C57BL/6 mice were induced by high fat diet. C57BL/6 mice were divided into three groups(normal, high fat diet with control, high fat diet with BPTS extract) and fed for 13weeks. Results : 1. The change of body weight and the final increase of body weight were decreased significantly. 2. The amount of the adipocyte in body weight was decreased significantly. 3. In primary adipocytes, ${\beta}3AR$ gene expression was increased significantly and leptin gene expression was decreased in $100{\mu}g/ml$ density significantly. 4. In primary adipocytes, the amount of TNF-${\alpha}$ was decreased in $100{\mu}g/ml$ density significantly and the amount of leptin was decreased but did not show significance. 5. In adipocytes tissue, the expression of leptin was decreased and the expression of ${\beta}3AR$ was increased. Conclusions : These results suggest that BPTS may inhibit the expression of the obese gene mad obese inhibitory about obese-mouse induced by high fat diet.

• 한국식품영양과학회지
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• 제30권2호
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• pp.325-330
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• 2001

The purpose of this study was to evaluate the obesity index and effect of dietary zinc and iron levels on serum trace minerals status in the high fat diet-induced obese rats. Male Sprague-Dawley rats were randomly assigned to control and high fat diet groups. Ten weeks later, the control and high fat diet group were rearranged into six groups by zinc and iron levels. After 16 wk serum zinc, iron, copper and manganese was analyzed. Obesity index was significantly higher in the group fed high fat diet (20% lard) than that of control group (5% corn oil). Body fat content was 12.10$\pm$4.51g/100g BW in high fat diet group and 7.64$\pm$4.18g/100g BW in control group. So, the obese rats were successfully induced by high fat diet. The trace mineral concentration of obese rats in serum were affected by zinc levels. Serum zinc concentration was increased by dietary zinc overload, whereas the iron, copper and manganese were decreased. Specially the manganese concentration was significantly affected by zinc levels. In both groups, serum trace mineral concentration was not changed significantly by the dietary iron levels. There were positive correlations between zinc, iron and manganese concentration according to dietary zinc and iron levels.

• Journal of Nutrition and Health
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• 제37권3호
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• pp.169-175
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• 2004

This study was performed to investigate the effect of acorn supplementation on the lipid profile and redox antioxidant enzyme activities in obese rat. Obesity in the rats was induced by feeding diet contained 10％ lard and 0.5％ cholesterol for 4 week. After 4 weeks, rats were divided into the following 5 groups; high fat diet (Control), high fat diet plus 10％ Acorn powder (APlO％), high fat diet plus 20％ Acorn powder (AP20％), high fat diet plus 0.2％ Acorn extract (AE0.2％), high fat diet plus 0.5％ Acorn extract (AE0.5％). Total food intake and food efficiency ratio (FER) was not significantly different by acorn powder and extract supplementation. But, body weight was decreased by 20％ acorn powder. Acorn powder and extract supplementation for 4 weeks tend to decrease total cholesterol and triglyceride level on the serum and hepatic tissue. There was no significant difference in hepatic glutathione (GSH) content among all the groups. The hepatic GST activity in acorn supplemented groups was lower than that of control. Glutathione peroxidase and catalase activities were higher in acorn supplemented groups than that of control. Hepatic TBARS levels of experimental groups were also significantly lower than that of control group. Our finding suggest that acorn powders and extract might have potential role for improving lipid profiles and antioxidant enzyme activities in obese rats.

• 대한한방소아과학회지
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• 제21권1호
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• pp.211-226
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• 2007

Objectives : These experimental studies were designed to investigate the effects of Bangpoongtongsungsankamibang on the weight and the lipid metabolism of the obese rats induced by 2% cholesterol diet. They were designed to change values in the serum and leptin levels in the rats. Materials and Methods : The serum and leptin levels were measured for a period of 8 weeks for obese SD rats induced by 2% cholesterol diet. Results : The body weight showed a tendency to decreased significantly in sample (p<0.05). The Total cholesterol and Triglyceride level significantly decreased in sample (p<0.05). The HDL Cholesterol levels significantly increased in sample (p<0.05). The LDH levels did not show a significant change in sample. The ALP levels significantly decreased in sample (p<0.05). The SGOT and SGPT level did not show a significant change in sample. The Leptin levels significantly decreased in sample (p<0.05). The average size of the epididymal fat cell significantly decreased in sample (p<0.05).

• 한국식품과학회지
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• 제52권2호
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• pp.144-148
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• 2020

Corn silk (Okmi-su) was anciently adopted as a material for tea or beverage. Corn silk extracts (CSE) contain bioactive phytochemicals such as phenolic acid, flavonoids, ascorbic acid, tannins, and glycosides. Under the impact of these functional components, CSE has benefits for antioxidation, diuresis, anti-diabetes, and dyslipidemia recovery. Nonetheless, its role in whole-body adiposity was not investigated; therefore, the effects of CSE on obesity were evaluated in high-fat diet-induced obese mice. Mice were assigned to either group (n=12); 1) normal diet (18% kcal from fat), 2) high-fat diet (45% kcal from fat, the control), 3) high-fat diet with CSE (800 mg/kg diet), and 4) high-fat diet with orlistat (500 mg/kg diet, a comparable control for weight loss). Our results showed that body weight, adiposity, and energy expenditure in obese mice were not altered by CSE. Lean body mass tended to decrease by CSE, which can be explained by stimulation of diuresis (p=0.06). In conclusion, our results suggest that dietary consumption of CSE does not influence the adiposity and underlying substrate utilization in high-fat diet-induced obese mice.

• Nutrition Research and Practice
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• 제5권1호
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• pp.11-19
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• 2011

Eotaxin is an important inflammatory chemokine in eosinophil chemotaxis and activation and, thus, is implicated in asthma. Recently, obesity was associated with an increased prevalence of asthma, but the relationship between obesity and eotaxin expression has only been partially understood in obese mice and human studies. Therefore, we studied the expression patterns of eotaxin in 3T3-L1 preadipocytes/adipocytes to determine whether eotaxin levels are influenced by body weight gain and/or reduction in diet-induced obese mice. First, we investigated eotaxin expression during differentiation in 3T3-L1 adipocytes. Then, we treated 3T3-L1 preadipoeytes/adipoeytes with tumor necrosis factor-alpha (TNF-${\alpha}$), eotaxin, interleukin (IL)-4, IL-5, or leptin. To examine the effects of weight loss in high-fat diet induced obese mice, we fed C57BL/6 mice a high-fat diet or a normal diet for 26 weeks. Then, half of the high-fat diet group were fed a normal diet until 30 weeks to reduce weight. Epididymal adipose tissue, visceral adipose tissue, serum, and bronchoalveolar fluid of mice were examined for eotaxin expression. The results showed that eotaxin expression levels increased with adipocyte differentiation and that more eotaxin was expressed when the cells were stimulated with TNF-${\alpha}$, eotaxin, IL-4, IL-5, or leptin. An in vivo study showed that eotaxin levels were reduced in visceral adipose tissues when high-fat diet fed mice underwent weight loss. Taken together, these results indicate a close relationship between eotaxin expression and obesity as well as weight loss, thus, they indirectly show a relation to asthma.

• 산업식품공학
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• 제22권4호
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• pp.358-365
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• 2018

통곡물 시리얼(Whole grain cereal, WGC)이 함유된 식이는 에너지 대사 조절에 중요한 다량영양소(macronutrients)를 제공한다. 본 연구는 고지방식이(high-fat diet, HFD)로 유발된 비만 마우스를 이용하여 WGC의 근감소성 비만 예방 효과에 대해 평가하였다. C57BL/6N 마우스에 정상식이(normal diet, ND), ND+WGC, HFD, HFD+WGC를 12주 동안 제공하였다. WGC는 체중, 식이효율, 체지방 및 지방세포의 크기를 감소시켰다. 또한, WGC는 간 무게 및 간에 축적된 지방을 감소시킴으로써 HFD에 의한 비알코올성 지방간을 개선시켰다. 더욱이, WGC는 비만 마우스 및 정상 마우스의 근육 무게 및 근력을 증가시켰다. 따라서, WGC는 지방 축적을 억제하고 근육량을 증가시키므로 근감소성 비만 예방을 위한 기능성 식품으로 사용될 수 있을 것으로 기대된다.

• 한국컴퓨터정보학회논문지
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• 제21권7호
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• pp.61-66
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• 2016

The frequency of obesity has risen dramatically in recent years but only few safe and effective drugs are currently available. In addition, obesity can induce type 2 diabetes (T2DM), hyperlipidemia and fatty liver disease. Recently, protective effect of purslane extract (PE) on obesity has been reported, but little is known about the role and mechanism of PE in obesity. This study aimed to evaluate the effect of PE on obesity and diabetes in obese mice. In addition, the effect of PE was compared with anti-obesity and diabetes drugs. High-fat diet (HFD)-induced obese mice were treated for 8 weeks with drugs as follows: PE, orlistat, metformin, voglibose or pioglitazone. While PE mixed with normal diet did not have any effects on BW in non-obese mice, PE mixed with HFD significantly reduced BW gain, insulin resistance, and glucose intolerance, without affecting food intake and appetite in obese mice. The effect was comparable to the effects of anti-obesity and diabetes drugs. Furthermore, PE significantly increased the activity of hepatocellular anti-oxidant enzymes, leading to protection of liver from oxidative stress in obese mice. These results suggest that PE treatment may be a useful tool for preventing obesity and complication of obesity.

• 한방비만학회지
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• 제21권2호
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• pp.49-58
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• 2021

Objectives: The purpose of this study was to investigate the effect of white pan bread added with Kamut (Triticum turgidum spp.) on high fat diet (HFD)-induced obese C57BL/6 mice. Methods: The white pan bread or white pan bread with Kamut (BK) were administered for 8 weeks in HFD-induced obese C57BL/6 mice. To evaluate the effect and its mechanisms of BK on obese mice, we measured body weight change, serum lipid profiles, histopathological analysis, and protein expression of CCAAT/enhancer binding protein-α (C/EBPα) in the liver. Results: Administration of BK significantly decreased body weight in HFD-induced obese mice. In addition, BK-administered group significantly reduced serum total cholesterol, glucose, and high-density lipoprotein cholesterol levels compared with the HFD-induced control group. The HFD-induced mice had damaged liver tissue and increased the size of adipose tissue, but BK-administered group attenuated liver damage and decreased the size of adipocyte. Furthermore, administration of BK significantly down-regulated C/EBPα in the liver compared with HFD-fed mice. In particular, BK-administered group has higher inhibited body weight, serum lipid profiles, and C/EBPα expressions than white pan bread-administered group. Conclusions: This study demonstrated that administration of BK attenuated HFD-induced obesity by regulation of C/EBPα than consumption of white pan bread. Therefore, BK could be developed as a bread for prevention of obesity.

• 생명과학회지
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• 제29권8호
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• pp.837-845
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• 2019

본 연구에서는 고지방식이로 유발된 비만 쥐의 골격근에서 유산소 운동과 레스베라트롤 투여가 미토콘드리아 생합성에 미치는 영향을 조사하였다. 4주령 C57BL/6의 수컷 쥐를 이용하여, 일반 식이 그룹(NC, n=10), 고지방식이 그룹(HR, n=10), 레스베라트롤 투여와 고지방식이 그룹(HRe, n=10), 유산소 운동 그룹(HE, n=10)으로 분류하였다. 유산소 운동은 16주 동안 40~60 min/day 동안 10-14m/min, 0% grade의 강도로 주당 4회 트레드밀 운동을 실시하였고, 레스베라트롤은 16주 동안 1일 1회, 주당 4회 체중 당 25 mg/kg을 투여하였다. COX-IV mRNA 발현은 NC와 HC 그룹 간에 유의한 차이가 있었으며(p<0.05), HE 그룹의 SIRT-3, $PGC-1{\alpha}$ 및 COX-IV mRNA 발현은 HC 및 HRe 그룹에 비해 유의하게 증가하였다(p<0.05). 또한, 오직 HE 그룹의 $PGC-1{\alpha}$ 및 COX-IV mRNA의 발현만이 HC 그룹에 비해 유의하게 증가하였다(p<0.05). 이상의 결과를 종합해보면, 고지방식이로 유발된 비만 쥐는 골격근에서 미토콘드리아 생합성 유전자 발현에 영향을 나타내지 않는 것으로 보인다. 하지만, 유산소 운동 훈련은 고지방식이로 유발된 비만 쥐의 골격근에서 미토콘드리아 생합성 유전자 발현을 증가시키는 것으로 나타났다. 이러한 연구 결과는 레스베라트롤 투여가 아닌 유산소 운동이 고지방식이로 유도된 쥐의 골격근에서 미토콘드리아 생합성에 긍정적인 영향을 미친다는 것을 시사한다.