• Journal of Microbiology and Biotechnology
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• 제19권2호
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• pp.155-160
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• 2009

Mast cells and basophils perform important functions as pivotal effector cells in IgE-mediated allergic reactions. KU812F cells, a human basophilic cell line isolated originally from chronic myelocytic leukemia, express a high affinity receptor of IgE, $Fc{\varepsilon}RI$. Kaempferol was extracted and isolated from a methanolic extract of flavonoid-rich Nelumbo nucifera stamens. In the present study, the inhibitory effects of kaempferol on $Fc{\varepsilon}RI$ expression in human basophilic KU812F cells was examined. Flow cytometric analysis revealed that $Fc{\varepsilon}RI$ expression on the cell surface was suppressed in a concentration-dependent manner when the cells were cultured with kaempferol. Moreover, RT-PCR analysis showed that the mRNA levels for $Fc{\varepsilon}RI$ ${\alpha}-\;and\;{\gamma}$-chains were reduced as the result of kaempferol treatment in a concentration-dependent manner. Kaempferol showed its suppressive effects on intracellular $Ca^{2+}$ concentration and histamine release from anti-$Fc{\varepsilon}RI$ ${\alpha}$-chain antibody-stimulated cells in a concentration-dependent manner. These observations indicate that kaempferol may exert antiallergic effect via down regulation of $Fc{\varepsilon}RI$ expression and degranulation.

• Journal of Ginseng Research
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• 제11권1호
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• pp.1-9
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• 1987

인삼추출물(95%, 50% 에탄올 및 수침액) ginsenoside Re 및 ginsana G115를 공식약물로 해서 thioacetamide로 intoxication 시킨 동물에 대한 in vitro, in vivo test를 통해 예방적 효과 및 치료적인 효과에 대해서 실험하였다. 항산화작용에 있어서 ginsenoside Re, 수침액, Ginsana C 115가 통계적으로 유효하였으며 zine sulfate turbidity test에서는 95% 에탄올 추출물과 ginsenoside Re가, GOT에 대해서는 Ginsana G 115, GPT에 대해서는 ginsenoside Re가 통계적인 유효성을 나타냈다. Venom toxin과 compound 48/80에 의한 mast cell의 탈과립현상에 대한 방어시험에서 50% 에탄올 추출물, 수침물, 95% 에탄올 추출물 및 Ginsana G 115가 유효하였다.

• Parasites, Hosts and Diseases
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• 제49권1호
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• pp.25-31
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• 2011

Cryptosporidium parvum is known as one of the most highly resistant parasites to gamma irradiation. To morphologically have an insight on the radioresistance of this parasite, ultrastructural changes in C. parvum sporozoites were observed after gamma irradiation using various doses (1, 5, 10, and 25 kGy) following a range of post-irradiation incubation times (10 kGy for 6, 12, 24, 48, 72, and 96 hr). The ultrastructures of C. parvum oocysts changed remarkably after a 10-kGy irradiation. Nuclear membrane changes and degranulation of dense granules were observed with high doses over 10 kGy, and morphological changes in micronemes and rhoptries were observed with very high doses over 25 kGy. Oocyst walls were not affected by irradiation, whereas the internal structures of sporozoites degenerated completely 96 hr post-irradiation using a dose of 10 kGy. From this study, morphological evidence of radioresistance of C. parvum has been supplemented.

• Journal of Applied Biological Chemistry
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• 제50권3호
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• pp.155-159
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• 2007

The water extracts of root, stem, and leaf from Korean indigenous Acanthopanax divaritacus were examined for their suppressive effects against allergic inflammations such as lipoxygenase activity, ${\beta}-hexosaminidase$ release, inflammatory cytokine production, and serum IgE level. The root extract inhibited the release of ${\beta}-hexosaminidase$, a degranulation marker, from rat basophilic leukemia cells (RBL-2H3) much more potently than the stem and leaf extracts. The root extract also significantly reduced the expression of $TNF-{\alpha}\;and\;IL-1{\beta}$ in the RBL-2H3 cells challenged with antigen. Moreover, there was a significant fall in the serum IgE level by the treatment of the root extract. Taken together, the root extract could be the most potent inhibitor of allergic inflammation, suppressing ${\beta}-hexosaminidase$ release and inflammatory cytokine expression, as well as reducing the rise of serum IgE level.

• Advances in Traditional Medicine
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• 제8권1호
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• pp.24-31
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• 2008

In the present investigation, we studied the effect of alcoholic extract of Moringa oleifera (M. oleifera) seed kernels on various experimental models of bronchial asthma. Significant (P < 0. 05) increase in preconvulsion time was observed due to pretreatment with M. oleifera when the guinea pigs were exposed to either acetylcholine (Ach) or histamine aerosol. This bronchodilating effect of M. oleifera was comparable to ketotifen fumarate. Spasmolytic effect of M. oleifera was also observed by dose dependent inhibition of ideal contractions induced by Ach, 5HT, histamine and $BaCl_2$. Alcoholic extract of M. oleifera produced significant dose dependent protection by egg albumin and compound 48/80 induced mast cell degranulation. Pretreatment with alcoholic extract of M. oleifera also decreased carrageenan induced rat paw edema, which was comparable to that of standard diclofenac sodium. Minimum inhibitory concentration for alcoholic extract of M. oleifera was low as compared to cold-water extract and hot water extract when antimicrobial activity was tested against various respiratory pathogens like Escherichia coli (E. coli), Staphylococus aureus (S. aureus) and pseudomonas aeruginosa (P. aeruginosa). Our data suggest that antiasthmatic activity of M. oleifera seed kernels may be due to its bronchodilator, anti-inflammatory, mast cell stabilization and antimicrobial activity.

• Journal of Ginseng Research
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• 제31권3호
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• pp.137-141
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• 2007

To evaluate the antiatopic effect of Korea Red Ginseng (RG, steamed root of Panax ginseng C.A. Meyer, Family Araliaceae), its inhibitory effect on passive cutaneous anaphylaxis reaction and itching in mice was measured. RG and its ingredient saponin fraction (SF) potently inhibited passive cutaneous anaphylaxis (PCA) reaction and scratching behaviors. RG at a dose of 100 mg/kg and SF at a dose of 50 mg/kg significantly inhibited the scratching frequency by 32% and 38%, respectively. RG and SF also inhibited the degranulation and protein expression of tumor necrosis factor $(TNF)-{\alpha}$ and interleukin (IL)-4 of RBL-2H3 cells induced by IgE-antign complex. However, polysaccharide fraction of RG did not inhibit it. Based on these findings, RG can improve allergic skin disorders atopic dermatitis and contact dermatitis by the regulation of $TNF-{\alpha}$, and IL-4 produced by mast cells and basophils and their membrane stabilization.

• 대한수의학회지
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• 제39권6호
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• pp.1168-1179
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• 1999

In order to study the effects of Jengjengamiyjintang on the duodenal ulcer induced by HCl-aspirin in rats, the changes of histological profiles, goblet cells(PAS-positive cells), and the distribution and frequency of cholecystokinin(CCK)-8 and serotonin-producing gastro-entero-endocrine cells were observed after oral administration of Jengjengamiyjintang. Histologically, very severe injury to duodenal epithelium were observed in control groups and these injuries were increased with time intervals. But in the Jengjengamiyjintang administrated groups, no gross lesion of ulcer were demonstrated and histologically minor injury to the mucosal epithelium were observed. PAS-positive cells were increased in the Jengjengamiyjintang administrated groups compared to that of control groups. Severe degranulation of CCK-8- and serotonin-immunoreactive cells were observed in control groups but these phenomenon was seldom in the Jengjengamiyjintang administrated groups. Serotonin-immunoreactive cells were significantly decreased in control groups but increased in Jengjengamiyjintang administrated groups compared with control groups. According to these result, it is suggested that Jengjengamiyjintang would accelerat the healing of the duodenal ulcer but the functional mechanisms were unknown.

• 한국식품영양과학회지
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• 제16권3호
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• pp.41-47
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• 1987

These studies were conducted to investigate effects of Prunus mume extract on experimentally alloxan-induced diabetes in rabbites, antibacterial activity and acute toxicity in mice ; 1. $LD_{50}$ of Prunus mume extract(P.M.E.) was 15.25g/kg intraperitoneally in mice. 2. P.M.E. showed more significant recuperative effect compared to the control group in alloxan-induced diabetes of rabbits. 1) P.M.E. 800mg/kg bodyweight exhibited more excellent hypoglycemic effect afte 6 days and adjacent to the normal level at 14th day. 2) SGPT activity was significantly decreased after 6 days, and the blood levels of total cholesterol and urea-nitrogen were significant in 800mg/kg at 6 days and 10 days respectively. 3. In antibacterial activity test P.M.E. was active at 0.195mg/kg in Staphyllococcus aureus, 3.125mg/ml in E. coli, Enterobacter cloacae, Klebsiella oxytoca, and in Pseudomonas aeruginosa at 0.391mg/ml. 4. In histological findings, the sample groups were deeply stained, fully granulated and partial degranulation of ${\beta}-cells$, and a few vacuolar and vesicular changes of cytoplasm than alloxan treated group in proportion to the sample amounts.

• 한국자원식물학회지
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• 제25권3호
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• pp.339-345
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• 2012

The marine carotenoid fucoxanthin can be found in marine brown seaweeds, macroalgae, diatoms, and microalgae, and has remarkable biological properties. Numerous studies have shown that fucoxanthin has considerable potential and promising applications in human health, but the underlying mechanisms involved in its anti-allergic activity are not fully understood. We here investigated the mechanisms by anti-allergic activity of fucoxanthin fraction from Eisenia bicyclis in immunoglobulin E-antigen complex (IgE/DNP-BSA)-stimulated RBL-2H3 mast cells. This study we found that the fucoxanthin inhibits the release of ${\beta}$-hexosaminidase and suppressed not only transcriptional activation of NF-${\kappa}B$, but also phosphorylation of ERK and JNK in IgE/DNP-BSA-treated RBL-2H3 cells. Fucoxanthin may be useful for preventing allergic diseases, including asthma and atopic dermatitis.

• 한방안이비인후피부과학회지
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• 제12권1호
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• pp.1-17
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• 1999

Shinisan has been used for treatment of allergic disease in Oriental Medicine. However, its effect in experimental models remains unknown. The mast cell plays a pivotal role in initiating allergic response by secreting intracytoplasmic granular mediators such as histamine. The present report describes an inhibitory effect of Shinisan on mast cell-mediated immediate type allergic reactions. Topical application of compound 48/80 can induce an ear swelling response in normal ($WBB6F_1-+/+$) mice but not in the congenic mast cell-deficient $WBB6F_1-W/W^v$ mice. Shinisan inhibited concentration-dependently mast cell-dependent ear swelling response induced by compound 48/80. Shinisan inhibited concentration-dependently passive cutaneous anaphylaxis induced by anti-dinitrophenyl (DNP) IgE in rats by topical application. Shinisan also inhibited concentration-dependently the histamine release from the rat peritoneal mast cells by compound 48/80 or anti-DNP IgE. Moreover, Shinisan had a significant inhibitory effect on compound 48/80-induced systemic anaphylactic reaction. These results indicate that Shinisan inhibits immediate-type allergic reactions by inhibition of mast cell degranulation in vivo and in vitro.