Browse > Article

Kaempferol Isolated from Nelumbo nucifera Stamens Negatively Regulates $Fc{\varepsilon}RI$ Expression in Human Basophilic KU812F Cells  

Shim, Sun-Yup (Institute of Marine Life Science, Pukyong National University)
Choi, Jae-Sue (Division of Food Science and Biotechnology, Pukyong National University)
Byun, Dae-Seok (Division of Food Science and Biotechnology, Pukyong National University)
Publication Information
Journal of Microbiology and Biotechnology / v.19, no.2, 2009 , pp. 155-160 More about this Journal
Abstract
Mast cells and basophils perform important functions as pivotal effector cells in IgE-mediated allergic reactions. KU812F cells, a human basophilic cell line isolated originally from chronic myelocytic leukemia, express a high affinity receptor of IgE, $Fc{\varepsilon}RI$. Kaempferol was extracted and isolated from a methanolic extract of flavonoid-rich Nelumbo nucifera stamens. In the present study, the inhibitory effects of kaempferol on $Fc{\varepsilon}RI$ expression in human basophilic KU812F cells was examined. Flow cytometric analysis revealed that $Fc{\varepsilon}RI$ expression on the cell surface was suppressed in a concentration-dependent manner when the cells were cultured with kaempferol. Moreover, RT-PCR analysis showed that the mRNA levels for $Fc{\varepsilon}RI$ ${\alpha}-\;and\;{\gamma}$-chains were reduced as the result of kaempferol treatment in a concentration-dependent manner. Kaempferol showed its suppressive effects on intracellular $Ca^{2+}$ concentration and histamine release from anti-$Fc{\varepsilon}RI$ ${\alpha}$-chain antibody-stimulated cells in a concentration-dependent manner. These observations indicate that kaempferol may exert antiallergic effect via down regulation of $Fc{\varepsilon}RI$ expression and degranulation.
Keywords
Kaempferol; Nelumbo nucifera stamens; $Fc{\varepsilon}RI$; intracellular $Ca^{2+}$; histamine; KU812F;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
연도 인용수 순위
1 Amoui, M., L. Drrova, P. Tolar, and P. Draber. 1997. Direct interaction of Syk and Lyn protein tyrosine kinases in rat basophilic leukemia cells activated via type Fc epsilon receptors. Eur. J. Immunol. 27: 321-328   DOI   ScienceOn
2 Cho, E. J., T. Yokozawa, D. Y. Rhyu, S. C. Kim, N. Shibahara, and J. C. Park. 2003. Study on the inhibitory effects of Korean medicinal plants and their main compounds on the 1,1- diphenyl-2-picrylhydrazyl radical. Phytomedicine 14: 544-551
3 Fujimura, Y., H. Tachibana, and K. Yamada. 2001. A tea catechin suppresses the expression of the high-affinity IgE receptor Fc$\varepsilon$RI in human basophilic KU812 cells. J. Agric. Food Chem. 49: 2527-2531   DOI   ScienceOn
4 Hakimi, J. C., J. A. Seals, L. Kondas, W. Pettine, W. Danko, and J. Kochan. 1990. The $\alpha$ subunit of the human IgE receptor (Fc$\varepsilon$RI) is sufficient for high affinity IgE binding. J. Biol. Chem. 265: 22079-22081   PUBMED
5 Huxley, R. R. and H. A. Neil. 2003. The relation between dietary flavonol intake and coronary heart disease mortality: A meta-analysis of propoective cohort diseases. Eur. J. Clin. Nutr. 57: 904-908   DOI   ScienceOn
6 Kishi, K. 1985. A new leukemia cell line with Philadelphia chromosome characterized as basophile precursors. Leuk. Res. 9: 381-390   DOI   PUBMED   ScienceOn
7 Nadler, M. J., S. A. Matthews, H. Turner, and J. P. Kinet. 2000. Signal transduction by the high-affinity immunoglobulin E receptor Fc$\varepsilon$RI coupling form to function. Adv. Immunol. 76: 325-355   PUBMED   ScienceOn
8 Nishiyama, C., T. Yokota, K. Okumura, and C. Ra. 1999. The transcription factors Elf-1 and GATA-1 to cell-specific enhancer elements of human high affinity IgE receptor $\alpha$-chain gene. J. Immunol. 163: 623 630   PUBMED
9 Schwartz, L. B., R. A. Lewis, D. Selden, and K. F. Austen. 1981. Acid hydrolases and tryptase from secretory granules of dispersed human lung mast cells. J. Immunol. 126: 1290-1294   PUBMED   ScienceOn
10 Shore, P. A., A. Burkhalter, V. H. Cohn, and H. C. Victor. 1959. A method for the fluorometric assay of histamine in tissues. J. Pharmacol. Exp. Ther. 127: 182-186   PUBMED   ScienceOn
11 Siraganian, R. P., J. Zhang, K. Suzuki, and K. Sada. 2002. Protein tyrosine kinase Syk in mast cell signaling. Mol. Immunol. 38: 1229-1233   DOI   ScienceOn
12 Fujimura, Y., H. Tachibana, M. Maeda-Yamamoto, M. T. Miyase, M. Sano, and K. Yamada. 2002. Antiallergic tea catechin, (-)-epigallocatechin-3-O-(3-O-methyl)-gallate, suppresses Fc$\varepsilon$RI expression in human basophilic KU812 cells. J. Agric. Food Chem. 50: 5729-5734   DOI   ScienceOn
13 Lim, S. S., Y. J. Jung, S. K. Hyun, Y. S. Lee, and J. S. Choi. 2006. Rat lens aldose reductase inhibitory constituents of Nelumbo nucifera stamens. Phytother. Res. 20: 825-830   DOI   ScienceOn
14 Nishiyama, C. 2006. Molecular mechanism of allergy-related gene regulation and hematopoietic cell development by transcription factors. Biosci. Biotechnol. Biochem. 70: 1-9   DOI   ScienceOn
15 Kinet, J. P. 1999. The high affinity IgE receptor (FcεRI): From physiology to pathology. Annu. Rev. Immunol. 17: 931-972   DOI   PUBMED   ScienceOn
16 Van Bergen, P. E., P. G. Hatcher, J. J. Boon, M. E. Collinson, and J. W. De Leeuw. 1997. Macromolecular composition of the propagule wall of Nelumbo nucifera. Phytochemistry 45: 601- 617   DOI   ScienceOn
17 Metzer, H. 1991. The high affinity receptor for IgE on mast cells. Clin. Exp. Immunol. 4: 269-279
18 La Cour, B., P. Molgaard, and Z. Yi. 1995. Traditional Chinese medicine in treatment of hyperlipidaemia. J. Ethnopharmacol. 46: 125-129   DOI   ScienceOn
19 Takahashi, K., C. Nishiyama, and C. Ra. 1999. Transcriptional factors Elf-1 and GATA-1 bind to cell-specific enhancer elements of human-affinity IgE receptor alpha-chain gene. J. Immunol. 163: 623-630   ScienceOn
20 Suzuki, Y., T. Yoshimura, T. Matsui, T. Inoue, O. Niide, S. Nunomura, and C. Ra. 2003. Fc(epsilon)RI signaling of mast cells activates intracellular production of hydrogen peroxide: Role in the regulation of calcium. J. Immunol. 171: 6119-6127   PUBMED   ScienceOn
21 Drombrowicz, D., V. Flamand, K. K. Brigman, B. H. Koller, and J. P. Kinet. 1993. Abolition of anaphylaxis by targeted disruption of the high affinity immunoglobulin E receptor $\varepsilon$ chain gene. Cell 75: 969-976   DOI   PUBMED   ScienceOn
22 Yono, S., H. Tachibana, and K. Yamada. 2005. Flavones suppress the expression of the high affinity IgE receptor Fc$\varepsilon$RI in human basophilic KU812F cells. J. Agric. Food Chem. 53: 1812-1817   DOI   ScienceOn
23 Middleton, E. J. 1998. Effect of plant flavonoids on immune and inflammatory cell function. Adv. Exp. Med. Biol. 439: 175-182   PUBMED
24 Nishiyama, C., M. Hasegawa, M. Nishiyama, K. Takahashi, Y. Akizawa, T. Yokota, K. Okumura, H. Ogawa, and C. Ra. 2002. Regulation of human Fc$\varepsilon$RI $\alpha$-chain gene expression by multiple transcription factors. J. Immunol. 168: 4546-4552   DOI   PUBMED
25 Hata, D., Y. Kawakami, N. Inagaki, C. S. Lantz, T. Kitamura, W. N. Khan, et al. 1998. Involement of Bruton's tyrosine kinase in Fc$\varepsilon$RI-dependent mast cell degranulation and cytokine production. J. Exp. Med. 187: 1235-1247   DOI   PUBMED
26 Blank, U., C. Ra, L. Miller, K. White, H. Metzer, and J. P. Kinet. 1989. Complete structure and expression in transfected cells of high affinity IgE receptor. Nature 337: 187-189   DOI   ScienceOn
27 Turner, H. and J. P. Kinet. 1999. Signalling through the highaffinity IgE receptor Fc epsilonRI. Nature 25: 24-30
28 Kinet, J. P., U. Blank, A. Brini, M. H. Jouvin, H. Kuster, O. Mejan, and C. Ra. 1991. The high affinity receptor for immunoglobulin E: A target for therapy of allergic diseases. Int. Arch. Allergy Appl. Immunol. 94: 51-55   DOI   PUBMED
29 Eiseman, E. and J. B. Bolen. 1992. Engagement of the highaffinity IgE receptor activates src protein-related tyrosine kinases. Nature 355: 78-80   DOI   ScienceOn
30 Nishiyama, C., T. Ito, M. Nishiyama, S. Masaki, K. Maeda, N. Nakano, et al. 2005. GATA-1 is required for expression of Fc{epsilon} RI on mast cells: Analysis of mast cells derived from GATA-1 knockdown mouse bone marrow. Int. Immunol. 17: 847-856   DOI   ScienceOn
31 Jung, H. A., J. E. Kim, H. Y. Chung, and J. S. Choi. 2003.Antioxidant principles of Nelumbo nucifera stamens. Arch.Pharm. Res. 26: 279-285   DOI   ScienceOn
32 Kim, H. K., H. R. Park, J. S. Lee, T. S. Chung, and H. Y. Chung. 2007. Down-regulation of iNOS and TNF-alpha expression by kaempferol via NF-kappaB inactivation in aged rat gingival tissues. Biogerontology 8: 399-408   DOI   ScienceOn