Browse > Article
http://dx.doi.org/10.3347/kjp.2011.49.1.25

Ultrastructural Changes in Cryptosporidium parvum Oocysts by Gamma Irradiation  

Joung, Mi-Gyo (Department of Environmental and Tropical Medicine, Konkuk University School of Medicine)
Yun, Se-Jeong (Department of Environmental and Tropical Medicine, Konkuk University School of Medicine)
Joung, Mi-Joung (Department of Environmental and Tropical Medicine, Konkuk University School of Medicine)
Park, Woo-Yoon (Department of Radiation Oncology, College of Medicine, Chungbuk National University)
Yu, Jae-Ran (Department of Environmental and Tropical Medicine, Konkuk University School of Medicine)
Publication Information
Parasites, Hosts and Diseases / v.49, no.1, 2011 , pp. 25-31 More about this Journal
Abstract
Cryptosporidium parvum is known as one of the most highly resistant parasites to gamma irradiation. To morphologically have an insight on the radioresistance of this parasite, ultrastructural changes in C. parvum sporozoites were observed after gamma irradiation using various doses (1, 5, 10, and 25 kGy) following a range of post-irradiation incubation times (10 kGy for 6, 12, 24, 48, 72, and 96 hr). The ultrastructures of C. parvum oocysts changed remarkably after a 10-kGy irradiation. Nuclear membrane changes and degranulation of dense granules were observed with high doses over 10 kGy, and morphological changes in micronemes and rhoptries were observed with very high doses over 25 kGy. Oocyst walls were not affected by irradiation, whereas the internal structures of sporozoites degenerated completely 96 hr post-irradiation using a dose of 10 kGy. From this study, morphological evidence of radioresistance of C. parvum has been supplemented.
Keywords
Cryptospotidium parvum; ultrastructure; gamma irradiation;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Brandes D, Sloan KW, Anton E, Bloedorn F. The effect of x-irradiation on the lysosomes of mouse mammary gland carcinomas. Cancer Res 1967; 27: 731-746.
2 Hamberg H, Brunk U, Ericsson JL, Jung B. Cytoplasmic effects of X-irradiation on cultured cells 2. Alterations in lysosomes, plasma membrane, Golgi apparatus, and related structures. Acta Pathol Microbiol Scand A 1977; 85: 625-639.
3 Hamberg H, Edman P. Induced autophagocytosis in macrophages. Origin of the segregating membranes. Acta Pathol Microbiol Immunol Scand A 1983; 91: 1-8.
4 Somosy Z, Takats A, Bognar G, Kovacs AL, Telbisz A, Racz A, Kovacs J, Köteles GJ. X-irradiation-induced changes of the prelysosomal and lysosomal compartments and proteolysis in HT-29 cells. Scanning Microsc 1996; 10: 1079-1090; discussion 1090-1091.
5 Bozzola J, Russell L. Electron Microscopy. Boston, USA. Jones and Bartlett Publishers. 1992, p 460-461.
6 Nanduri J, Williams S, Aji T, Flanigan TP. Characterization of an immunogenic glycocalyx on the surfaces of Cryptosporidium parvum oocysts and sporozoites. Infect Immun 1999; 67: 2022-2024.
7 Petry F, Robinson HA, McDonald V. Murine infection model for maintenance and amplification of Cryptosporidium parvum oocysts. J Clin Microbiol 1995; 33: 1922-1924.
8 Sato C, Kojima K, Mishizawa K. Recovery from radiation-induced decrease in cell membrane charge by added adenosine triphosphate and its modification by colchicine or cytochalasin B. Biochem Biophys Res Commun 1975; 67: 22-27.   DOI   ScienceOn
9 McClain DE, Trypus CA, May L. Effect of gamma radiation on membrane fluidity of MOLT-4 nuclei. Radiat Res 1990; 123: 263-267.   DOI   ScienceOn
10 Montgomery PO, Karney D, Reynolds RC, McClendon D. Cellular and subcellular effects of ionizing radiations. Am J Pathol 1964; 44: 727-746.
11 Djaczenko W, Starzyk H, Rzucidlo Z. X-ray irradiation induced changes of the nuclear membrane of Kirkman-Robbins tumour cells. Experientia 1973; 29: 83-84.   DOI   ScienceOn
12 Somosy Z, Thuroczy G, Kubasova T, Kovács J, Szabo LD. Effects of modulated and continuous microwave irradiation on the morphology and cell surface negative charge of 3T3 fibroblasts. Scanning Microsc 1991; 5: 1145-1155.
13 Barham SS, Walters RA. X-irradiation-induced nuclear lesions in cultured mammalian cells: an ultrastructural analysis. Radiat Res 1978; 76: 105-126.   DOI   ScienceOn
14 Harris JR, Adrian M, Petry F. Structure of the Cryptosporidium parvum microneme: a metabolically and osmotically labile apicomplexan organelle. Micron 2003; 34: 65-78.   DOI   ScienceOn
15 Kergonou JF, Braquet M, Rocquet G. Influence of whole-body gamma irradiation upon rat liver mitochondrial fractions. Radiat Res 1981; 88: 377-384.   DOI   ScienceOn
16 Erickson GA, Koppenol WH. Effects of gamma-irradiation on isolated rat liver mitochondria. Int J Radiat Biol Relat Stud Phys Chem Med 1987; 51: 147-155.   DOI
17 Tetley L, Brown SM, McDonald V, Coombs GH. Ultrastructural analysis of the sporozoite of Cryptosporidium parvum. Microbiology 1998; 144 (Pt 12): 3249-3255.   DOI   ScienceOn
18 O'Donoghue PJ. Cryptosporidium and cryptosporidiosis in man and animals. Int J Parasitol 1995; 25: 139-195.   DOI   ScienceOn
19 Nichols GL. Food-borne protozoa. Br Med Bull 2000; 56: 209-235.   DOI   ScienceOn
20 Mac Kenzie WR, Hoxie NJ, Proctor ME, Gradus MS, Blair KA, Peterson DE, Kazmierczak JJ, Addiss DG, Fox KR, Rose JB, Davis JP. A massive outbreak in Milwaukee of Cryptosporidium infection transmitted through the public water supply. N Engl J Med 1994; 331: 161-167.   DOI   ScienceOn
21 Kato S, Jenkins MB, Ghiorse WC, Bowman DD. Chemical and physical factors affecting the excystation of Cryptosporidium parvum oocysts. J Parasitol 2001; 87: 575-581.   DOI
22 Yu JR, Park WY. The effect of gamma-irradiation on the viability of Cryptosporidium parvum. J Parasitol 2003; 89: 639-642.   DOI   ScienceOn
23 Lee SU, Joung M, Nam T, Park WY, Yu JR. Quantitative evaluation of infectivity change of Cryptosporidium parvum after gamma irradiation. Korean J Parasitol 2009; 47: 7-11.   DOI
24 DuPont HL, Chappell CL, Sterling CR, Okhuysen PC, Rose JB, Jakubowski W. The infectivity of Cryptosporidium parvum in healthy volunteers. N Engl J Med 1995; 332: 855-859.   DOI   ScienceOn
25 Lee SU, Joung M, Nam T, Park WY, Yu JR. Rejoining of gamma-ray-induced DNA damage in Cryptosporidium parvum measured by the comet assay. Exp Parasitol 2010; 125: 230-235.   DOI   ScienceOn
26 Yang S, Healey MC. The immunosuppressive effects of dexamethasone administered in drinking water to C57BL/6N mice infected with Cryptosporidium parvum. J Parasitol 1993; 79: 626-630.   DOI   ScienceOn