• The Korean Journal of Mycology
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• v.49 no.1
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• pp.33-44
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• 2021

Lentinula edodes (Berk.) Pegler, the most produced mushroom in the world, is an edible mushroom with very high nutritional and pharmacological value. Currently, interest in the protection of genetic resources is increasing worldwide, and securing the distinction between new cultivars is very important. Therefore, the development of efficient molecular markers that can discriminate between L. edodes cultivars is required. In this study, we developed cleaved amplified polymorphic sequence (CAPS) markers for the identification of L. edodes cultivars (Sanbaekhyang and Sulbaekhyang). These markers were developed from whole genome sequencing data from L. edodes monokaryon strain B17 and resequencing data from 40 cultivars. A nucleotide deletion existed in scaffold 19 POS 214449 in Sanbaekhyang (GT→G), and a single nucleotide polymorphism changed in scaffold 7 POS 215801 in Sulbaekhyang (G→A). The restriction enzymes Hha I and HpyCH4IV distinguished Sanbaekhyang and Sulbaekhyang, respectively, from other cultivars. Thus, we developed two CAPS markers for the identification of the L. edodes cultivars Sanbaekhyang and Sulbaekhyang.

• Journal of Plant Biotechnology
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• v.48 no.1
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• pp.12-17
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• 2021

Saponarin found in young barley sprouts has a variety of beneficial biological and pharmacological properties, including antioxidant, hypoglycemic, antimicrobial, and hepatoprotective activities. Our previous work demonstrated that saponarin content was correlated with the expression levels of three biosynthetic pathway genes [chalcone synthase (HvCHS1), chalcone isomerase (HvCHI), and UDP-Glc:isovitexin 7-O-glucosyltransferase (HvOGT1)] in young barley seedlings under various abiotic stress conditions. In this study, we investigated the saponarin content and expression levels of three saponarin biosynthetic pathway genes in hulled and hulless domestic barley cultivars. In the early developmental stages, some hulled barley cultivars (Kunalbori1 and Heukdahyang) had much higher saponarin contents than did the hulless barley cultivars. An RNA expression analysis showed that in most barley cultivars, decreased saponarin content correlated with reduced expression of HvCHS1 and HvCHI, but not HvOGT1. Heat map analysis revealed both specific increases in HvCHS1 expression in certain hulled and hulless barley cultivars, as well as general changes that occurred during the different developmental stages of each barley cultivar. In summary, our results provide a molecular genetic basis for the metabolic engineering of barley plants to enhance their saponarin content.

• Journal of Practical Agriculture & Fisheries Research
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• v.20 no.1
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• pp.175-185
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• 2018

This study was conducted to evaluate the physiological and ecological characters of rice cultivars suitable for low fertilized condition. 5 rice cultivars(Jinmibyeo, Sobibyeo, Hwayeongbyeo, Nagdongbyeo and Junambyeo) were cultivated for selection under 3 different nitrogen application levels, and 1 cultivars were selected. The results obtained are summarized as follows ; High yielded rice cultivars under low N application level were Junambyeo, Jinheng and Sobibyeo. Also these cultivars were yielded highly under conventional level(11kg/10a). Milled rice yield under conventional level(11kg/10a) was positively correlated with them under low N levels. Milled rice yield was most affected by no. of grain/m². Rice cultivars that were high crop growth rate(CGR) before heading stage were Junambyeo, Sobibyeo and Nagdongbyeo. Grain filling rate was increased mostly until 20 days after heading, and decreased after this stage. Nitrogen use efficiency was higher under low N level(5.5kg/10a) than conventional level(11kg/10a). Especially, Junambyeo was most low in Apparent recovery of applied N(AR) under low N application level, but most high in Agronomic N use efficiency(ANUE). This characteristics of Junambyeo will to be useful for selection of variety suitable for growing under low fertilized condition.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.4
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• pp.249-256
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• 2007

This experiment was conducted to compare the agronomic characteristics and productivity in introduced hairy vetch cultivars and developed new Korean Hairy vetch cultivars in two areas of the experimental field of Grassland and Forage Crops Division, National Institute of Animal Science from 2005 to 2006. The experiment was arranged in randomized complete block design with three replications. A total of 11 hairy vetch cultivars used in this study including 9 introduced cultivars (Penn-02, Sander, Latigo, Welta, Ostsaat, VV4712, Minnie, Barlosa and Capello) and 2 Korean cultivars (Cold green and Cheong pa). The early flowering varieties of Minnie, Barlosa, Capello and Cold green were grown as early maturity cultivars, while the late flowering varieties of Penn-02, Sander, Latigo, Welta, Ostsaat, VV4712 and Cheong pa were grown as medium and late maturity. The winter hardiness of hairy vetch was good except for Minnie, Barlosa and Capello of early maturity cultivars. In this study, dry matter yield of VV4712 was the highest of 11 hairy vetch cultivars. And dry matter yield of medium and late maturity vetch increased in the middle region while that of early maturity increased in the south. The new Korean cultivar, Cheong pa, as a medium and late maturity hairy vetch was not bad compared to introduced cultivars and Cold green as an early maturity hairy vetch was an excellent cultivar compared to introduced cultivars in both regions. Latigo in NDF (neutral detergent fiber) and Welta in ADF (acid detergent fiber) were lower than other cultivars, while Sander in IVDMD (in vitro dry matter digestibility) and CP (crude protein) content were higher than other cultivars. The results of this experiment indicated that the productivity of hairy vetch cultivars was highest in VV4712, and Cheong pa and Cold green of new Korean cultivar were the promising cultivars of hairy vetch in Korea.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.3
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• pp.203-213
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• 2017

This study investigated the changes in yields, antioxidant compounds, and antioxidant activities among 11 adzuki bean cultivars cultivated in a paddy field with somewhat poorly drained soil. The adzuki bean cultivars were cultivated in the paddy field from 2015 to 2016 in Milyang, Gyeongsangnam, Korea. Average soil moisture content was 16.5% in 2015 and 31.0% in 2016 at the experimental site during adzuki bean cultivation. As the soil moisture content increased, most of the adzuki bean cultivars showed deceases in stem height, first setting pod node, number of pods, 100 seed weight, and yield. Chungju-pat produced the greatest yields among the 11 cultivars in 2015 and 2016, whereas Hongeon had the smallest yields. Adzuki bean yields after paddy field cultivation was lower for all cultivars than for adzuki bean yields from the same cultivars after upland field cultivation. Chungju-pat and Chilbo-pat showed the smallest decreases in yields after paddy field cultivation, whereas Hongeon and Kumsil showed the greatest yield reductions. There were no significant differences in proximate composition. Some mineral components ($P_2O_5$, Ca, and Mg) were statistically different across cultivars. Chungju-pat had the highest Ca and Mg contents, but antioxidant components (polyphenol and flavonoids) and antioxidant activities (ABTS and DPPH) were highest in Saegil and lowest in Jungbu-pat. PCA and clustering analyses, based on the growth, yield, and antioxidant component measurements, performed to identify which variables contributed the most to separating adzuki bean cultivars or to grouping cultivars with similar characteristics. These analyses showed that the antioxidant components and antioxidant activities had the most influence on grouping cultivars together. Among the 11 cultivars, Saegil was statistically different from the other cultivars, but the other 10 cultivars were not significantly different under paddy field cultivation. Soil moisture content affected adzuki bean yield and antioxidant component contents. An increase in soil moisture led to a decrease in yield, but an increase in antioxidant components. These results provide information that will improve the selection of an appropriate adzuki bean cultivar for use in paddy fields.

• Journal of the Korean Institute of Landscape Architecture
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• v.42 no.2
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• pp.77-85
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• 2014

This study was conducted to examine the morphological characteristics of those Azalea Cultivars(hereinafter, Azalea Cultivars) that are mainly used for landscaping in Korea in order to prepare classification criteria. As testing materials, major Azalea Cultivars cultivated in large quantities by its producing companies were collected. Thereafter, the qualitative and quantitative characteristics of the traits of Azalea Cultivars were investigated in the way of characteristic investigation for new cultivars of azalea used by the Korea Seed and Variety Service in order to classify them and prepare the classification criteria. Since cultivar names have not been established for Azalea Cultivars for landscaping thus far, the data were compiled using the names used by cultivating companies. According to the results, Azalea Cultivars cultivated in Suncheon, Jeonnam mainly for landscaping were 10 in number; Beni, Daewang, Three, Zasanhong, Hancheol, Sancheoljuk, Gyeobsancheoljuk, Baekcheoljuk, Akado, and Seok-am. Among them, the cultivars Beni, Daewang, and Three could not be easily distinguished from each other because they are commonly called Yeongsanhong by cultivating companies and the shapes of their leaves and flowers are similar to each other. In particular, the flower color of Beni was 'bright red', that of Daewang was 'vivid purple', and that of Three was 'bright purple'. In addition, Zasanhong and Hancheol were similar to each other in shape the degree of expression of spots on the flowers and the gloss on the front side of the leaves of Hancheol were higher and stronger compared to that of Zasanhong. Sancheoljuk flowered in early April, earlier compared to other Azalea Cultivars. Gyeobsancheoljuk is an elementary species of Sancheoljuk and it had double flowers although all other traits were similar to those of Sancheoljuk. Although Baekcheoljuk was easily distinguished because it had white flowers, its leaves were similar to those of Akado the reason why these two cultivars could not be easily distinguished from each other. The cultivar Akado flowered early May later compared to other Azalea Cultivars and its flowers were relatively large in diameter as with Baekcheoljuk and Sancheoljuk. Finally, the cultivar Seok-am was easily distinguished because it had smaller leaves compared to other cultivars and it flowered late as with the cultivar Akado.

• Horticultural Science & Technology
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• v.33 no.6
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• pp.947-954
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• 2015

To select suitable spray chrysanthemum cultivars for Agrobacterium-mediated transformation, thirty-nine (39) spray cultivars bred in the National Institutes of Korea and a standard cultivar Jinba from Japan were collected and tested for regeneration rate and Agrobacterium infection assays. MS medium with $0.5mg{\cdot}L^{-1}$ IAA and $1.0mg{\cdot}L^{-1}$ BAP was used for shoot regeneration from leaf disks and internodes. The shoot regeneration rate in leaf disks was the highest in cultivar BRM, followed by cultivars VS, WW and YTM. The cultivar JB (Jinba) used as a transformation material in previous reports ranked similarly to cultivars PK and SPP. In shoot regeneration from internodes, the shoot regeneration rate was the highest for cultivar PA, followed by cultivar WW. The infection rate of leaves and internodes of 40 chrysanthemum cultivars with agrobacterium was investigated. Cultivars WPP, YNW, VS, PP, WW, FA, PA and YMN showed the highest infection levels in leaves, whereas cultivars WPP, PA, PK and YNW had the highest infection levels in internodes. Considering all of these results, cultivars VS and WW were the most appropriate for gene transformation of chrysanthemum using leaves, while cultivar PA was for internodes.

• Horticultural Science & Technology
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• v.31 no.3
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• pp.371-379
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• 2013

Anthracnose disease caused by Colletotrichum orbiculare, induces severe damage to cucurbits worldwide. Resistance of 112 commercial cultivars of cucurbits to C. orbiculare was evaluated. Seedlings of each cultivar at 2- to 3-leaf stage were inoculated with C. orbiculare KACC 40809 by spraying spore suspension of the fungus at a concentration of $4.0{\times}10^5$ spores/mL. Among the 36 cultivars of cucumber, 'Asiastrike', 'Tongilbaedadagi', 'Daeseon', 'Cheongrokmatjjang', 'Nokyacheongcheong', and 'Asianogak' were moderately resistant and the others were susceptible. All the tested cultivars of melon (33) and watermelon (4) showed highly susceptible response to C. orbiculare. On the other hand, the squash cultivars (17) represented less susceptibility to the fungus than the other cucurbits. Of the squash cultivars, 'Gammirak' and 'Teotbat' were resistant and 12 cultivars were moderately resistant. Among the rootstocks for cucurbits, ten cultivars including 'JjeuyakaEX', 'Nunbusyeo', 'Union', 'RS111', 'Ganggeuntoza', 'Hwangjaetoza', 'NO.8', 'Shintoza', 'Bulpaetoza', and 'Newtype' showed high resistance to the anthracnose pathogen. From the results, the resistant cultivars could be used as sources of resistance to cucurbits anthracnose (C. orbiculare) in the future breeding programs.

• Korean Journal of Weed Science
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• v.17 no.3
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• pp.325-333
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• 1997

Selection of pepper (Capsicum sp.) cultivars tolerant to acetolactate synthase (ALS)-inhibiting herbicides {imazethapyr, 2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-5-ethyl-3=pyridine-carboxylic acid, and primisulfuron methyl 2-[[[[[4,6-bis(difluoromethoxy)-2-pyrimidinyl]amino] carbonyl]amino]sulfonyl]benzoate} was investigated. Pepper cultivars such as Red Top, Happy Dry, Golden Tower, and Hagyeorae showed relatively tolerant response to imazethapyr, while cultivars; Korea, Cheongyang, Oriental Glory, and Hanam were susceptible. Red Horn, Jopoong, Kwangbok, and Wangcho cultivars were tolerant to primisulfuron whereas Korea, Dahhong, Chamjoah, and Poongchon cultivars were susceptible. Determination of growth inhibition by ALS-inhibiting herbicides showed that the $I_{50}$ estimates of growth from the susceptible- and tolerant-cultivars were 0.075 and 0.20kg ai/ha for imazethapyr; 0.06 and 0.16kg ai/ha for primisulfuron, respectively. Furthermore, the $GR_{50}$ estimates of growth from the susceptible and tolerant cultivars were 0.05 and 0.20kg ai/ha for imazethapyr; 0.07 and 0.16kg ai/ha for primisulfuron, respectively. This result, based on the $GR_{50}$ and $I_{50}$ values, indicates that responses of pepper to ALS-inhibiting herbicides between tolerant- and susceptible-cultivars were different about 3- to 4-fold to imazethapyr, and 2- to 3-fold to primisulfuron.

• Journal of Plant Biotechnology
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• v.45 no.2
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• pp.90-101
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• 2018

Strawberry (Fragaria ${\times}$ ananassa) is a globally-cultivated and popular fruit crop, prized for its flavor and nutritional value. Sweetness, a key determinant of fruit quality, depends on the sugar composition and concentration. We selected eight strawberry cultivars based on the fruit soluble solids content to represent high and low sugar content groups. The average soluble solid content was $13.6^{\circ}Brix$ (Okmae, Geumsil, Aram, and Maehyang) and $2.9^{\circ}Brix$ (Missionary, Camino Real, Portola, and Gilgyung53), for the high and low sugar content groups, respectively. Sucrose was the main sugar in the cultivars with high sugar content, whereas fructose was the main component in the low sugar content cultivars. Fruit starch concentration ranged from $3.247{\pm}0.056$ to $3.850{\pm}0.055g/100g$, with a 12% higher concentration in the high sugar content cultivars. Additionally, we identified 41 sugar metabolism-related genes in Fragaria ${\times}$ ananassa and analyzed the relationship between their transcripts and the sugar accumulation in fruit. FaGPT1, FaTMT1, FaHXK1, FaPHS1, FaINVA-3, and FacxINV2-1 were highly expressed in the high sugar content cultivars, while FapGlcT, FaTMT2-1, FaPHS2-1, FaSUSY1-1, and FaSUSY1-2 were highly expressed in the low sugar content cultivars. In general, a greater number of genes encoding sugar transporters or involved in sugar synthesis were highly expressed in the high sugar content cultivars. Contrarily, genes involved in sugar degradation were preferentially transcribed in the low sugar content cultivars. Although gene expression was not perfectly proportional to sugar content or concentration, our analysis of the genes involved in sugar metabolism and accumulation in strawberries provides a framework for further studies and for the subsequent engineering of sugar metabolism to enhance fruit quality.