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http://dx.doi.org/10.4489/KJM.20210004

Development of Cleaved Amplified Polymorphic Sequence Markers of Lentinula edodes Cultivars Sanbaekhyang and Sulbaekhyang  

Moon, Suyun (Department of Biology, Chungbuk National University)
Hong, Chang Pyo (Theragen Bio)
Ryu, Hojin (Department of Biology, Chungbuk National University)
Lee, Hwa-Yong (Department of Forest Science, Chungbuk National University)
Publication Information
The Korean Journal of Mycology / v.49, no.1, 2021 , pp. 33-44 More about this Journal
Abstract
Lentinula edodes (Berk.) Pegler, the most produced mushroom in the world, is an edible mushroom with very high nutritional and pharmacological value. Currently, interest in the protection of genetic resources is increasing worldwide, and securing the distinction between new cultivars is very important. Therefore, the development of efficient molecular markers that can discriminate between L. edodes cultivars is required. In this study, we developed cleaved amplified polymorphic sequence (CAPS) markers for the identification of L. edodes cultivars (Sanbaekhyang and Sulbaekhyang). These markers were developed from whole genome sequencing data from L. edodes monokaryon strain B17 and resequencing data from 40 cultivars. A nucleotide deletion existed in scaffold 19 POS 214449 in Sanbaekhyang (GT→G), and a single nucleotide polymorphism changed in scaffold 7 POS 215801 in Sulbaekhyang (G→A). The restriction enzymes Hha I and HpyCH4IV distinguished Sanbaekhyang and Sulbaekhyang, respectively, from other cultivars. Thus, we developed two CAPS markers for the identification of the L. edodes cultivars Sanbaekhyang and Sulbaekhyang.
Keywords
Cleaved amplified polymorphic sequence (CAPS) marker; Genome wide; Indel; Lentinula edodes; Single nucleotide polymorphism (SNP);
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