• Journal of Veterinary Clinics
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• v.24 no.1
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• pp.51-55
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• 2007

A 13-year-old male Yorkshire terrier was Presented with Persistent weight loss anorexia and dark brown urine of 3-month duration. On physical examination, a firm oval mass was palpated at left renal region. In hematology and blood chemistry, neutrophilia, anemia, thrombocytopenia and elevation of ALKP were found. Abdominal radiography, ultrasonography and ultrasound-guided percutaneous pyelogram revealed masses originated from left kidney, mildly dilated renal Pelvis and intact ureter. Urinalysis showed hematuria and proteinuria. Because the state of dog became deteriorated during transfusion and the frail renal tumor was suspected to be the cause of inflammation and anemia, nephrectomy was performed. Renal masses, approximately $2{\times}3cm\;and\;5{\times}4cm$ respectively in size, was surrounded by swollen and congested mesentery and ascites. Metastatic lesion was not found in other organs. During recovery, the dog showed cardiopulmonary arrest and did not respond to critical care. Histologically the kidney was affected by necrotic and hemorrhagic change. This hemangiosarcoma most likely arose from the renal parenchyma resulting In diffuse lesions in the kidneys thought to be the cause of chronic anorexia and weight loss.

• Molecules and Cells
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• v.26 no.1
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• pp.26-33
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• 2008

The plasmid pJB01, a member of the pMV158 family isolated from Enterococcus faecium JC1, contains three open reading frames, copA, repB, and repC. Plasmids included in this family produce counter-transcribed RNA (ctRNA) that contributes to copy number control. The pJB01 ctRNA, a transcript which consists of 54 nucleotides (nts), is encoded on the opposite strand from the copA/repB intergenic region and partially overlaps an atypical ribosome binding site (ARBS) for repB. The ARBS is integrated by the two underlined conserved regions: 5'-TTTTTGTNNNNTAANNNNNNNNNATG-3', and the ctRNA is complementary only to the 5' conserved sequence 5'-TTTTTGT-3'. This complementary sequence is located at a distance from the terminal loop of the ctRNA secondary structure. The ctRNA structure predicted by the mfold program suggests the possible generation of a terminal and an internal hairpin loop. The amount of in vitro translation product of repB mRNA was inversely proportional to the ctRNA concentration. Mutations in the terminal and internal hairpin loops of the ctRNA had inhibitory effects on its binding to the target mRNA. We propose that the intact structures of the terminal and internal hairpin loops, respectively, play important roles in forming the initial kissing and extending complexes between the ctRNA and target mRNA and that these regulate the copy number of this plasmid.

• Proceedings of the KIEE Conference
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• 1994.07b
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• pp.1536-1539
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• 1994

The influence due to metallic particle contaminated on spacer surface is remarkable in the decreasing of dielectric strength in $SF_6$ GIS. In relation with this problem, We studied, AC flash-over voltage characteristics and breakdown mechanism are investigated under metallic particle initiated condition in $SF_6$ gas by varying the particle position, particle shape with a plane-plane electrode. The main results arc as follows 1. The small amount of the metallic particle in the gap do not make flash-over voltage to be influence, but the significant decrease of th flash-overed voltage is result in case of the big and long size of the metallic paraticle. 2. Influence of the flash-over voltage are lowest in the mid and are highest in the electrode of metallic particle position. 3. In case of the initiated metallie particle, The more the pressure are high, the more the recluced ratio of flash-over voltage are high. 4. The metallic particle shape which results in the reduced flash-over voltage forced the critical pressure to move in to the region of low pressure. 5. The existance of the metallic particle on the upper electrode side and high pressure make the decreasing ratio of flash-over voltage bigger than that of the ground side electrode.

• Microbiology and Biotechnology Letters
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• v.38 no.4
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• pp.475-480
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• 2010

Actinomycetes are Gram-positive soil bacteria and one of the most important industrial microorganisms due to superior biosynthetic capabilities of many valuable secondary metabolites as well as production of various valuable bioconversion enzymes. Among them are cytochrome P450 hydroxylase (CYP), which are hemoproteins encoded by a super family of genes, are universally distributed in most of the organisms from all biological kingdoms. Actinomycetes are a rich source of soluble CYP enzymes, which play critical roles in the bioactivation and detoxification of a wide variety of metabolite biosynthesis and xenobiotic transformation. Cyclosporin A (CyA), one of the most commonly-prescribed immunosuppressive drugs, was previously reported to be hydroxylated at the position of 4th N-methyl leucine by a rare actinomycetes called Sebekia benihana, leading to display different biological activity spectrum such as loss of immunosuppressive activities yet retaining hair growth-stimulating side effect. In order to improve this regio-selective CyA hydroxylation in S. benihana, previously-identified several secondary metabolite up-regulatory genes from Streptomyces coelicolor and S. avermitilis were heterologously overexpressed in S. benihana using an $ermE^*$ promoter-containing Streptomyces integrative expression vector. Among tested, SCO4967 encoding a conserved hypothetical protein significantly stimulated region-specific CyA hydroxylation in S. benihana, implying that some common regulatory systems functioning in both biosynthesis and bioconversion of secondary metabolite might be present in different actinomycetes species.

• Journal of Integrative Natural Science
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• v.2 no.2
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• pp.131-167
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• 2009

Global warming is regarded as one of the most critical issues that should be taken care of by the entire global community as it threatens the survival of mankind. South Korea, in particular, undergoes faster warming than the average rate of global warming. South Korea has revealed various warming rates and trends being surrounded by sea on three sides and having complex terrains dominated by mountains. The rates vary according to regions and their urbanization and industrialization. Differences also derive from seasons and weather elements. Changes to the highest, mean, and lowest temperature are also different according to the characteristics of regions and observatories, which is more apparent where the force of artificial weather applies. In an urban area, temperature gaps tend to decrease as the lowest temperature rises more than the highest temperature. Meanwhile, temperature gaps grow further in a coastal or country region where the force of artificial weather is small and the force of natural weather prevails. In this study, the investigator analyzed the changes to the weather elements of 11 observation spots that had gone through no changes in terms of observation environment since 1961, were consecutively observed, and had the quality of their observation data monitored on an ongoing basis. Using the results, I tried to identify natural and artificial causes affecting certain spots. Located on the east coast of the Asian Continent, South Korea sees weather changing very dynamically. Having huge influences on our weather, China has achieved very rapid industrialization for the last 30 years and produced more and more greenhouse gases and air pollution due to large-size development projects. All those phenomena affect our weather system in significant ways. Global warming continues due to various reasons with regional change differences. Thus the analysis results of the study will hopefully serve as basic data of weather statistics with which to set up countermeasures against climate changes.

• Journal of ILASS-Korea
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• v.5 no.3
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• pp.17-26
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• 2000

The main parameter commonly used to evaluate spray distribution is spray angle. Spray angle is important because it influences the axial and radial distribution of the fuel. Spray angles were measured and compared for the two non-air assisted injectors such as 2hole-2stream 4hole-1stream injectors used for port fuel injection gasoline engines with n-heptane as a fuel by three different measuring techniques, i.e., digital image processing, shadowgraphy, and spray patternator, respectively. Fuel was injected with the injection pressures of 0.2-0.35 MPa into the room temperature and atmospheric pressure environment. In digital image processing approach, the selection of the transmittance level is critical to obtain the edge of spray and hence to measure the spray angle. From the measurement results by the shadowgraphy technique, it is dear that the spray angle is varied during the spray injection period. The measurement results from spray patternator show that the different spray angles exist in different region. Spray angle increases with the increase in the injection pressure. it is suggested that the spray angle and stream separated angle should be specified when spray is characterized for 2hole-2stream injector, because spray angle is much different though stream separated angle is same. It was also considerably affected by the measurement techniques introduced in this experimental work. However, the optimal axial distance for measuring the spray angle seems to be at least 60-80 mm from the injector tip for two non-air assisted injectors.

• Journal of the Korean Chemical Society
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• v.34 no.6
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• pp.663-672
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• 1990

The extraction of ginseng saponins with near critical or supercritical ammonia(SCF-NH$_3$) was carried out at 80$^{\circ}C$-160$^{\circ}C$ and ammonia densities of 339.8-525 $mg/cm^3$. In order to evaluate brownish color of white ginseng extracts, a spetrophotometric method was applied in ultraviolet and visible range. The extractibilities of ginseonoside $Rb_1$, -$Rb_2$, -Rc, -Rd, -Re, -Rf, $-Rg_1$, and $-Rg_2$ were determined by high performance liquid choromatography. The best extractability was 7.36% at 133$^{\circ}C$ and 403 $\pm$ 24.605 $mg/cm^3$. In the case of the high extraction temperature, it is thought that extraction times can be reduced and the selectivity of protopanaxatriol can be increased. The brownish color of extracts is affected by temperature and extractability. When extraction temperature is between 132$^{\circ}C$ and 140$^{\circ}C$, it is shown that the range is the retrograde region where extractablility decrease with increasing temperature.

• Proceedings of the Korea Concrete Institute Conference
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• 2008.04a
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• pp.65-68
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• 2008

This paper verified the lateral resisting capacity of CFT column-RC flat plate connection in comparison with general RC column-flat plate connection and detected moment capacity and ductility capacity of connection according to lateral force-displacement ratio. We made and tested specimens which have different variables respectively and as a result derive a following conclusion. In CFT specimen a critical section was extended and initial stiffness and moment increased 35%, 25$^{\sim}$35% respectively in comparison to general RC column specimen. In all specimens generally shear governed behaviors and in CFT specimen complemented with seismic band, flexure behavior region of slab was extended and also ductility ratio and energy absorptance increased.

• Journal of the Korean Chemical Society
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• v.48 no.6
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• pp.561-567
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• 2004

The influence of ultrasound frequency, dissolved gases, and initial concentration on the decomposition of sodium dodecylbenzene sulfonate(DBS) aqueous solution was investigated using ultrasound generator with 200 W ultrasound power. The decomposition rates at three frequencies(50, 200, and 600 kHz) examined under argon atmosphere were highest at 200 kHz. The highest observed decomposition rate at 200 kHz occurred in the presence of oxygen followed by air and argon, helium, and nitrogen. The effect of initial concentration of DBS on the ultrasonic decomposition was decreased with increasing initial concentration and would depend upon the formation of micelle in aqueous solution. It appears that the ultrasound frequency, dissolved gases, and initial concentration play an important role on the sonolysis of DBS. Sonolysis of DBS mainly take place at the interfacial region of cavitation bubbles by both OH radical attack and pyrolysis to alkyl chain, aromatic ring, and headgroup.

• Steel and Composite Structures
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• v.34 no.1
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• pp.123-139
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• 2020

The tube outward local buckling of Concrete-Filled Steel Tube (CFST) beam under high compression stress is still considered a critical problem, especially for steel tubes with a slender section compared to semi-compact and compact sections. In this study, the flexural performance of stiffened slender cold-formed square tube beams filled with normal concrete was investigated. Fourteen (14) simply supported CFST specimens were tested under static bending loads, stiffened with different shapes and numbers of steel stiffeners that were provided at the inner sides of the tubes. Additional finite element (FE) CFST models were developed to further investigate the influence of using internal stiffeners with varied thickness. The results of tests and FE analyses indicated that the onset of local buckling, that occurs at the top half of the stiffened CFST beam's cross-section at mid-span was substantially restricted to a smaller region. Generally, it was also observed that, due to increased steel area provided by the stiffeners, the bending capacity, flexural stiffness and energy absorption index of the stiffened beams were significantly improved. The average bending capacity and the initial flexural stiffness of the stiffened specimens for the various shapes, single stiffener situations have increased of about 25% and 39%, respectively. These improvements went up to 45% and 60%, for the double stiffeners situations. Moreover, the bending capacity and the flexural stiffness values obtained from the experimental tests and FE analyses validated well with the values computed from equations of the existing standards.