• 제목/요약/키워드: chemical analysis tests

검색결과 422건 처리시간 0.029초

High-Throughput In Vitro Screening of Changed Algal Community Structure Using the PhotoBiobox

  • Cho, Dae-Hyun;Cho, Kichul;Heo, Jina;Kim, Urim;Lee, Yong Jae;Choi, Dong-Yun;Yoo, Chan;Kim, Hee-Sik;Bae, Seunghee
    • Journal of Microbiology and Biotechnology
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    • 제30권11호
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    • pp.1785-1791
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    • 2020
  • In a previous study, the sequential optimization and regulation of environmental parameters using the PhotoBiobox were demonstrated with high-throughput screening tests. In this study, we estimated changes in the biovolume-based composition of a polyculture built in vitro and composed of three algal strains: Chlorella sp., Scenedesmus sp., and Parachlorella sp. We performed this work using the PhotoBiobox under different temperatures (10-36℃) and light intensities (50-700 μmol m-2 s-1) in air and in 5% CO2. In 5% CO2, Chlorella sp. exhibited better adaptation to high temperatures than in air conditions. Pearson's correlation analysis showed that the composition of Parachlorella sp. was highly related to temperature whereas Chlorella sp. and Scenedesmus sp. showed negative correlations in both air and 5% CO2. Furthermore, light intensity slightly affected the composition of Scenedesmus sp., whereas no significant effect was observed in other species. Based on these results, it is speculated that temperature is an important factor in influencing changes in algal polyculture community structure (PCS). These results further confirm that the PhotoBiobox is a convenient and available tool for performance of lab-scale experiments on PCS changes. The application of the PhotoBiobox in PCS studies will provide new insight into polyculture-based ecology.

격막형 PSD를 적용한 소형 추진기관 설계 및 시험평가 (Design, Test and Evaluation on the PSD with Thermal Barrier Type for Subscale Dual Pulse Rocket Motor)

  • 김진용;권태하;이원복;조원만;이방업;윤남균;이영우
    • 한국추진공학회지
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    • 제18권6호
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    • pp.82-87
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    • 2014
  • 이중펄스 로켓모타는 두 개의 펄스모타 사이에 격벽 또는 격막을 통하여 1단 펄스모타 연소 후 2단 펄스모타가 연소하는 방식으로 일회성 추력 방식이 아닌 효율적으로 이중추력을 조절한다. 이러한 추력 배분을 통하여 사거리와 종말속도를 증가시켜 미사일의 기동성 및 사거리를 향상시킨다. 본 연구에서는 격막 펄스분리장치를 갖는 소형 이중펄스모타에 설계하였고 지상연소시험을 통하여 특성을 분석하였다. 결과적으로 얻어진 데이터 값들은 향후 F-type 이중펄스모타의 설계에 적용가능할 것으로 판단된다.

등전환법과 등온 DSC를 이용한 고에너지 물질의 정밀 반응모델 개발 (An Extraction of Detailed Isoconversional Kinetic Scheme of Energetic Materials using Isothermal DSC)

  • 김유천;박정수;권국태;여재익
    • 한국추진공학회지
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    • 제20권2호
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    • pp.46-55
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    • 2016
  • Differential Scanning Calorimetry(DSC) 실험 데이터를 이용하여 고에너지 물질의 반응속도식을 추출해내는 이론적 방법을 제안하고 알루미늄 고함유 화약(RDX/HTPB/Al)에 대한 반응속도식 추출을 수행하였다. DSC 실험 결과는 Friedman 등전환법으로 분석되었다. 질량분율에 따른 활성화에너지와 빈도인자를 추출해 내어 반응속도식을 완성하였다. 추출된 반응속도식은 고에너지 물질의 화학반응과정을 몇 단계의 주요단계로 가정하는 형태가 아닌 전체 화학반응 과정을 나타내는 형태를 갖는다. 이는 기존의 ODTX 실험을 통해 추출되는 화학반응속도식 형태에 비해 이론적 측면과 정확성 측면에서 상당한 장점을 갖는다. 추출된 반응속도식의 검증을 위해 화학반응률 그리고 생성물 질량분율에 대해 DSC 실험과 동일한 조건하에서 전산모사를 수행하였으며 실험값과 잘 일치함을 확인하였다. 또한 완속가열 전산모사를 수행하였으며 실험결과와 비교하여 DSC 반응속도식의 전산모사에의 적용가능성을 확인하였다.

Influence of Compost Recycling and Magnesium Supplement on Physical and Chemical Traits of Animal Manure Compost

  • Lee, Jin-Eui;Kwag, Jung-Hoon;Ra, Chang-Six
    • Journal of Animal Science and Technology
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    • 제52권6호
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    • pp.513-519
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    • 2010
  • A series of experiments were performed to study the influence of the following parameters on the physical traits and composition of swine manure compost: (1) addition of magnesium (Mg) at a molar ratio of 1.2 with respect to $PO_4$, and (2) reutilization of compost containing $MgNH_4PO_4{\cdot}6H_2O$ (magnesium ammonium phosphate, MAP). Three independent batch tests were conducted for replication: batch test I-control (C) and Mg added (T), batch test II-C, T and compost recycle ($T_{R1}$), and batch test III-C, T and compost recycle ($T_{R2}$). Magnesium addition and compost reutilization had no adverse effect on the degradation of organic matter. Reuse of the compost, however, had a clear effect on the total nitrogen (TN) and total phosphorus (TP) contents in the final compost. Repeated compost reutilization as a bulking material was resulted in composts rich in N and P. Upon adding the Mg supplement to the composting materials, the ortho-phosphate (OP) to TP ratio decreased due to the MAP crystallization reaction. The decrease in the OP/TP ratio and the increase in the TP content of the compost indicate that water-soluble phosphate is converted into a slow-release phosphate by the formation of crystals during composting. X-ray diffraction analysis of the irregular shaped crystals in the compost indicated that they are MAP crystals and that the crystallization of MAP begins immediately after the addition of the Mg supplement. The Mg addition to composting materials and the reutilization of compost as a bulking material would be a practical means to conserve nutrient content.

Comparative In Vitro Biological Toxicity of Four Kinds of Air Pollution Particles

  • Shin, Han-Jae;Cho, Hyun Gi;Park, Chang Kyun;Park, Ki Hong;Lim, Heung Bin
    • Toxicological Research
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    • 제33권4호
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    • pp.305-313
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    • 2017
  • Accumulating epidemiological evidence indicates that exposure to fine air pollution particles (APPs) is associated with a variety of adverse health effects. However, the exact physiochemical properties and biological toxicities of fine APPs are still not well characterized. We collected four types of fine particle (FP) (diesel exhaust particles [DEPs], natural organic combustion [NOC] ash, synthetic organic combustion [SOC] ash, and yellow sand dust [YSD]) and investigated their physicochemical properties and in vitro biological toxicity. DEPs were almost entirely composed of ultrafine particles (UFPs), while the NOC, SOC, and YSD particles were a mixture of UFPs and FPs. The main elements in the DEPs, NOC ash, SOC ash, and YSD were black carbon, silicon, black carbon, and silicon, respectively. DEPs exhibited dose-dependent mutagenicity even at a low dose in Salmonella typhimurium TA 98 and 100 strains in an Ames test for genotoxicity. However, NOC, SOC, and YSD particles did not show any mutagenicity at high doses. The neutral red uptake assay to test cell viability revealed that DEPs showed dose-dependent potent cytotoxicity even at a low concentration. The toxicity of DEPs was relatively higher than that of NOC, SOC, and YSD particles. Therefore, these results indicate that among the four FPs, DEPs showed the highest in vitro biological toxicity. Additional comprehensive research studies such as chemical analysis and in vivo acute and chronic inhalation toxicity tests are necessary to determine and clarify the effects of this air contaminant on human health.

LT-WGS 반응을 위한 Cu/ZnO/MgO/Al2O3 촉매의 수분처리에 의한 촉매 특성 분석 (Catalytic Characteristic of Water-Treated Cu/ZnO/MgO/Al2O3 Catalyst for LT-WGS Reaction)

  • 박지혜;백정훈;조광희;;이광복
    • 한국수소및신에너지학회논문집
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    • 제30권2호
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    • pp.95-102
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    • 2019
  • In order to investigate the effect of water treatment on activity of WGS catalyst, $Cu/ZnO/MgO/Al_2O_3$ (CZMA) catalysts were synthesized by co-precipitation method. The prepared catalysts were water-treated at two different temperature (250, $350^{\circ}C$). Synthesized catalysts were characterized by using BET, SEM, $N_2O$ chemisorption, XRD, $H_2-TPR$ and XPS analysis. The catalytic activity tests were carried out at a GHSV of $28,000h^{-1}$ and a temperature range of $180-320^{\circ}C$. The reduction temperature decreased with water treatment and CZMA_250 catalyst showed the lowest reduction temperature and retained a large amount of $Cu^+$. Water-treated catalysts showed increased reactivity compared to untreated catalyst and the CZMA_250 catalyst showed higher catalytic activity on WGS reaction.

금속연료-피복재 상호확산 거동에 미치는 기상증착법의 영향 (Effect of Vapor Deposition on the Interdiffusion Behavior between the Metallic Fuel and Clad Material)

  • 김준환;이병운;이찬복;지승현;윤영수
    • 대한금속재료학회지
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    • 제49권7호
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    • pp.549-556
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    • 2011
  • This study aimed to evaluate the performance of diffusion barriers in order to prevent fuel-cladding chemical interaction (FCCI) between the metallic fuels and the cladding materials, a potential hazard for nuclear fuel in sodium-cooled fast reactors. In order to prevent FCCI, Zr or V metal is deposited on the ferritic-martensitic stainless steel surface by physical vapor deposition with a thickness up to $5{\mu}m$. The diffusion couple tests using uranium alloy (U-10Zr) and a rare earth metal such as Ce-La alloy and Nd were performed at temperatures between 660~800$^{\circ}C$. Microstructural analysis using SEM was carried out over the coupled specimen. The results show that significant interdiffusion and an associated eutectic reaction ocurred in the specimen without a diffusion barrier. However, with the exception of the local dissolution of the Zr layer in the Ce-La alloy, the specimens deposited with Zr and V exhibited superior eutectic resistance to the uranium alloy and rare earth metal.

IN-VITRO STUDY OF CO2 EXTRACT OF TERMINALIA CHEBULA IN BREAST CANCER CELL LINE MD-MBA-231

  • Chandil, Shachi;Bamoriya, Harikishan;More, D.B.
    • 셀메드
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    • 제11권3호
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    • pp.16.1-16.7
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    • 2021
  • Cancer is an abnormal growth of cells in body which leads to death. These cells are born due to imbalance in cell proliferation mechanism. In 2018, WHO released new statistics on cancer incidence, mortality, and prevalence worldwide i.e., GLOBOCAN 2018 estimates for 28 types of cancer in which more prevalence of cervix and breast cancer. According to survey, in India about 7.8 million cancer deaths and 11.5 million new cases arise in 2018, which will increase to 19.3 million new cases per year by 2025. Though breast cancer as such is not explained anywhere in Ayurvedic compendia, correlations can be done with the Stana Arbuda. Ayurveda, the ancient system of medicine came into existence 1000's of years ago with an objective of maintaining the health of people and treating diseases. Many herbs used in Ayurveda have been screened for activity against cancer and in-vitro and in-vivo studies have given promising leads. The plant, called as "Mother of Medicine", Haritaki has been extensively studied for its various ailments because of its extraordinary healing potency. Haritaki (Terminalia chebula Retz.), Family: Combretaceae have a great therapeutic value and is widely distributed in India. Dried fruit of Terminalia chebula contains high quantities phenolic compounds consist of ellagic acid, gallic acid and chebulic acid. The fruit extract of T. chebula is having different biological properties like anticancer, antioxidant, hepatic and renal protective activities etc. In this study, we focus on the use of CO2 extract of Terminalia chebula, on the breast cancer cell line MDA-MB-231. All tests proved that CO2 extract of Terminalia chebula containing active chemical component, therefore our experiment showed the positive results for CO2 extract of Terminalia chebula against breast cancer cell line cancer MDA-MB-231. The MTT assay results were used to evaluate the anti-cancer activity of the extract. The percentage of cell growth and cell viability were calculated from tabulated result values of MTT assay. Cell viability MTT assay also showed significant growth inhibition, at the same time statistical analysis of MTT assay also proved significant results.

그래핀 옥사이드 혼입 고강도 시멘트 모르타르의 Interfacial Transition Zone (ITZ) 특성에 관한 연구 (Investigation on the Characteristics of Interfacial Transition Zone (ITZ) of High-Strength Cement Mortar Incorporating Graphene Oxide)

  • 임수민;조성민;유준성;임승민;배성철
    • 한국건설순환자원학회논문집
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    • 제10권3호
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    • pp.343-350
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    • 2022
  • 최근 취성재료인 콘크리트의 강도 발현에 가장 불리하게 작용하는 영역인 골재와 시멘트 복합체 사이 Interfacial transition zone (ITZ) 성능 개선을 위해 나노 실리카, 탄소나노튜브, 그래핀 옥사이드(GO) 등 나노물질을 활용한 방안이 제시되고 있다. 나노물질 중에서 우수한 분산성을 가진 GO는 ITZ 영역에 높은 비율로 존재하는 Ca2+과 화학적 결합을 형성하여 일반강도 콘크리트 내 ITZ 성능 개선에 효과적인 것으로 보고되었다. 본 연구에서 미소수화열 분석 및 Scanning electron microscope 이미지 분석 기법을 활용하여 도출한 GO 혼입에 따른 수화 발열량 변화와 ITZ의 두께 변화 및 표준사 주변 공극 분포 변화를 통해 GO가 고강도 시멘트 모르타르 내 ITZ 특성에 미치는 영향을 조사하였다.

LPBF용 타이타늄 합금 분말의 유변특성에 대한 입자 구형도의 영향 (Effect of Particle Sphericity on the Rheological Properties of Ti-6Al-4V Powders for Laser Powder Bed Fusion Process)

  • 김태윤;강민혁;김재혁;홍재근;유지훈;이제인
    • 한국분말재료학회지
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    • 제29권2호
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    • pp.99-109
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    • 2022
  • Powder flowability is critical in additive manufacturing processes, especially for laser powder bed fusion. Many powder features, such as powder size distribution, particle shape, surface roughness, and chemical composition, simultaneously affect the flow properties of a powder; however, the individual effect of each factor on powder flowability has not been comprehensively evaluated. In this study, the impact of particle shape (sphericity) on the rheological properties of Ti-6Al-4V powder is quantified using an FT4 powder rheometer. Dynamic image analysis is conducted on plasma-atomized (PA) and gas-atomized (GA) powders to evaluate their particle sphericity. PA and GA powders exhibit negligible differences in compressibility and permeability tests, but GA powder shows more cohesive behavior, especially in a dynamic state, because lower particle sphericity facilitates interaction between particles during the powder flow. These results provide guidelines for the manufacturing of advanced metal powders with excellent powder flowability for laser powder bed fusion.